Evaluation of Resistance, Enzymatic Response, and Phenolic Compounds in Roots of F1 Cucumber Hybrids to Fusarium Oxysporum F. Sp. Radicis-Cucumerinum

AbstractCucumber (Cucumis sativus L.) is widely cultivated in many parts of the world. Its production is significantly affected by Fusarium root and stem rot, which is caused by the fungus Fusarium oxysporum f. sp. radicis-cucumerinum, a major disease of cucumber in some regions. Most of the management programs for the control of Fusarium root and stem rot are not successful; therefore, growing resistant cultivars may be the best solution. Use of disease-resistant cultivars is a key to environmentally friendly and economically sustainable disease control in modern crop production. To find resistance sources, 10 F1cucumber hybrids were screened against F. oxysporum f. sp. radicis-cucumerinum. Total phenolic content (PC), soluble peroxidase (POX), and polyphenol oxidase (PPO) activities were evaluated at 0, 4, and 8 days after inoculation. Significant differences were observed between resistant and susceptible genotypes in increase of total PCs and enzymatic activities at fourth day after inoculation. These findings can be used in breeding programs of cucumber aiming at creating F1cultivars resistant to Fusarium root and stem rot.

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The chemical composition and in vitro digestibility of pistachio by-product

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200021268 ◽

2007 ◽

Vol 2007 ◽

pp. 223-223 ◽

Cited By ~ 5

Author(s):

Atiyeh Bohluli ◽

Abasali Naserian ◽

Reza Valizadeh ◽

Fereydon Eftekarshahroodi

Keyword(s):

Organic Matter ◽

Chemical Composition ◽

Total Phenolic Content ◽

Dry Matter ◽

Phenolic Content ◽

Total Phenolic ◽

In Vitro Digestibility ◽

Pistachio Nuts ◽

The World

Pistachio (pistachio vera) is from Anacardiaceae family. According to the FAO (2003) report, Iran is the largest pistachio producer in the world (more than 310,000 tons). The most three important exported pistachio nuts of Iran are Ohadi, Akbari and Kaleghuchi (Mohammadi, 2005). About 150,000 tons in DM of pistachio by-product (PB) is produced from dehulling process in Iran, annually. This by-product is mainly consisted of pistachio hulls (PH), and then peduncles, leaves and a little amount of mesocarp and kernels. In this experiment the chemical composition, Total Phenolic Content (TPC) and tannin amount of PB and PH of Ohadi, Kaleghuchi and white varieties were determined; also, In Vitro Dry Matter and Organic Matter Digestibility (IVDMD and IVOMD) were measured.

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Dynamics of total phenolic content in different apple tissues and genotypes: impacts and relevance for breeding programs

Scientia Horticulturae ◽

10.1016/j.scienta.2014.01.020 ◽

2014 ◽

Vol 168 ◽

pp. 58-63 ◽

Cited By ~ 6

Author(s):

D.F. Holderbaum ◽

T. Kon ◽

M.P. Guerra

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Breeding Programs

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Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [Brassica juncea (L.) Czern & Coss.]

Genes ◽

10.3390/genes12111784 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1784

Author(s):

Manjeet Singh ◽

Ram Avtar ◽

Nita Lakra ◽

Ekta Hooda ◽

Vivek K. Singh ◽

...

Keyword(s):

Oil Quality ◽

Indian Mustard ◽

Proteome Analysis ◽

Stem Rot ◽

Sclerotinia Stem Rot ◽

Breeding Programs ◽

Resistant Genotype ◽

Effective Utilization ◽

Resistant Cultivars ◽

Important Disease

Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P1, P2, F1, F2, BC1P1, and BC1P2) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding programs to develop resistant cultivars against this devastating disease.

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Quality and safety evaluation of new tomato cultivars

Italian Journal of Food Science ◽

10.15586/ijfs.v33i2.1921 ◽

2021 ◽

Vol 33 (2) ◽

pp. 35-45 ◽

Cited By ~ 1

Author(s):

Mattia Rapa ◽

Salvatore Ciano ◽

Laura Gobbi ◽

Roberto Ruggieri ◽

Giuliana Vinci

Keyword(s):

Safety Assessment ◽

Phenolic Content ◽

Safety Evaluation ◽

Data Matrix ◽

Total Phenolic ◽

Quality And Safety ◽

Multivariate Statistical ◽

Breeding Programs ◽

Organoleptic Characteristics ◽

Tomato Cultivars

Tomato (Solanum lycopersicum) is a dietary source of bioactive compounds and breeding programs continuously create new cultivars with different nutritional and organoleptic characteristics. The aim of this work is to provide a quality and safety assessment of new tomato cultivars: Bamano, Dulcemiel and Sugarland. Eight biogenic amines, total phenolic content and antioxidant activity (DPPH and ABTS assays) have been determined. Tyramine was not detected in any samples. Sugarland was characterized by a high content of serotonin (266.87 ± 11.16 mg/kg) and phenolic compounds (303.15 ± 21.62 mgGAE/kg). Moreover, multivariate statistical analyses were applied to the data matrix.

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Influence of 1-Methylcyclopropene Treatment on Postharvest Quality of Four Scab (Venturia inaequalis)-Resistant Apple Cultivars

Journal of Food Quality ◽

10.1155/2017/5951041 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Moises Zucoloto ◽

Kang-Mo Ku ◽

Moo Jung Kim ◽

Mosbah M. Kushad

Keyword(s):

Antioxidant Capacity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Venturia Inaequalis ◽

Postharvest Quality ◽

Total Phenolic ◽

Storage Duration ◽

Resistant Cultivars ◽

Golden Delicious

Scab (Venturia inaequalis) is a very serious disease for apples causing up to 80% of loss in yield but there are only a few studies on postharvest quality of scab-resistant cultivars. In this study we evaluated the effect of 1-methylcyclopropene (1-MCP) on fruit quality, total phenolic content, and antioxidant capacity after storage of four scab-resistant cultivars and compared to a standard cultivar, “Golden Delicious.” In general, ethylene production and respiration rates significantly differed among cultivars, between control and 1-MCP-treated fruits, and between storage duration regimes. 1-MCP treatment retarded fruit softening and lowered juice pH but storage effect on soluble solids and acidity depended on cultivar and 1-MCP treatment. Total phenolic content was significantly affected by storage duration and 1-MCP treatment. Antioxidant capacity of the four scab-resistant cultivars was either similar to or significantly higher than that of “Golden Delicious” with the 1-MCP-treated fruits having significantly higher antioxidant capacity than the nontreated fruits after storage. Our results clearly show that the quality of four scab-resistant cultivars was comparable to that of “Golden Delicious” and 1-MCP effect differed among cultivars. These differences need to be considered in developing storage regime to minimize quality deterioration during long-term storage.

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Environmental conditions affect phenolic content and antioxidant capacity of leaves and fruit in wild partridgeberry (Vaccinium vitis-idaea)

Botany ◽

10.1139/cjb-2016-0041 ◽

2016 ◽

Vol 94 (7) ◽

pp. 509-521 ◽

Cited By ~ 6

Author(s):

Zobayer Alam ◽

Hugo R. Morales ◽

Julissa Roncal

Keyword(s):

Antioxidant Capacity ◽

Newfoundland And Labrador ◽

Phenolic Content ◽

Total Phenolic ◽

Wild Populations ◽

Breeding Programs ◽

Pharmaceutical Ingredients ◽

The North ◽

Water Ph ◽

Selection Of

Partridgeberry (Vaccinium vitis-idaea L.) is a good source of food and pharmaceutical ingredients, for which cultivation interest is increasing in North America. Nutrition-oriented breeding programs will benefit from an understanding of how the environment affects the biochemical traits of interest in wild populations. Total phenolic content (TPC) and antioxidant capacity (AC) as measured by the ability to capture free radicals were evaluated simultaneously in leaves and fruit of 56 wild populations across Newfoundland and Labrador, Canada. We tested variation in TPC and AC as a function of eight environmental factors, which showed different effects in leaves and fruit. Contrary to our expectations, TPC was not correlated with AC in either leaves or fruit, and mean TPC and AC were higher in fruit than in leaves. We propose a series of environment-based models for the selection of wild populations. Models for fruit involved ecoregion, temperature, and coastal proximity, and explained up to 51% of variation. While leaf models included surface water pH and sensitivity to acid rain, explaining up to 31%. We conclude that wild population selection in the province should target the North Shore Forest ecoregion and warm temperatures for fruit; and regions with low water alkalinity and pH > 6.6 for leaves.

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Correlations of Morphological, Anatomical, and Chemical Features of Grape Berries with Resistance to Botrytis cinerea

Phytopathology ◽

10.1094/phyto.2003.93.10.1263 ◽

2003 ◽

Vol 93 (10) ◽

pp. 1263-1273 ◽

Cited By ~ 91

Author(s):

Franka Mlikota Gabler ◽

Joseph L. Smilanick ◽

Monir Mansour ◽

David W. Ramming ◽

Bruce E. Mackey

Keyword(s):

Botrytis Cinerea ◽

Phenolic Content ◽

Total Phenolic ◽

Berry Skin ◽

Resistant Cultivars ◽

Grape Berries ◽

Skin Protein ◽

Before And After ◽

Cell Layers ◽

Moderately Resistant

Resistance of mature berries of grapevine cultivars and selections to postharvest infection by Botrytis cinerea was assessed. Little or no resistance existed in most popular table grape Vitis vinifera cultivars, except in moderately resistant ‘Emperor’ and ‘Autumn Black’. Highly resistant grapes were V. rotundifolia, V. labrusca, or other complex hybrids. Morphological, anatomical, and chemical characteristics of 42 genetically diverse cultivars and selections with various levels of resistance to B. cinerea were examined to determine which features were associated with resistance. We quantified the (i) density of berries within a cluster; (ii) number of pores and lenticels on the berry surface; (iii) thickness and number of cell layers in the epidermis and external hypodermis; (iv) amount of cuticle and wax; (v) berry skin protein content; (vi) total phenolic content of the skin before and after B. cinerea inoculation; and (vii) catechin and trans- and cis-resveratrol contents of the skin before and after inoculation. The number of pores was negatively correlated with resistance. Highly resistant cultivars had few or no pores in the berry surface. The number and thickness of epidermal and hypodermal cell layers and cuticle and wax contents were positively correlated with resistance. Other characteristics evaluated were not associated with resistance. trans-Resveratrol and cis-resveratrol were induced by B. cinerea inoculation only in sensitive and moderately resistant cultivars and selections.

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Phenolic Profiling of Five Different Australian Grown Apples

Applied Sciences ◽

10.3390/app11052421 ◽

2021 ◽

Vol 11 (5) ◽

pp. 2421

Author(s):

Heng Li ◽

Vigasini Subbiah ◽

Colin J. Barrow ◽

Frank R. Dunshea ◽

Hafiz A. R. Suleria

Keyword(s):

Phenolic Compounds ◽

Malus Domestica ◽

Phenolic Acid ◽

Phenolic Content ◽

Antioxidant Potential ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

The World ◽

Flavonoid Quercetin

Apples (Malus domestica) are one of the most widely grown and consumed fruits in the world that contain abundant phenolic compounds that possess remarkable antioxidant potential. The current study characterised phenolic compounds from five different varieties of Australian grown apples (Royal Gala, Pink Lady, Red Delicious, Fuji and Smitten) using LC-ESI-QTOF-MS/MS and quantified through HPLC-PDA. The phenolic content and antioxidant potential were determined using various assays. Red Delicious had the highest total phenolic (121.78 ± 3.45 mg/g fw) and total flavonoid content (101.23 ± 3.75 mg/g fw) among the five apple samples. In LC-ESI-QTOF-MS/MS analysis, a total of 97 different phenolic compounds were characterised in five apple samples, including Royal Gala (37), Pink Lady (54), Red Delicious (17), Fuji (67) and Smitten (46). In the HPLC quantification, phenolic acid (chlorogenic acid, 15.69 ± 0.09 mg/g fw) and flavonoid (quercetin, 18.96 ± 0.08 mg/g fw) were most abundant in Royal Gala. The obtained results highlight the importance of Australian apple varieties as a rich source of functional compounds with potential bioactivity.

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Catechin Composition and Antioxidant Activity of Black Teas in Relation to Brewing Time

Journal of AOAC International ◽

10.5740/jaoacint.17-0235 ◽

2017 ◽

Vol 100 (6) ◽

pp. 1694-1699 ◽

Cited By ~ 15

Author(s):

Wojciech Koch ◽

Wirginia Kukula-Koch ◽

Kazimierz Głowniak

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Black Tea ◽

Total Phenolic ◽

Ionization Time ◽

Tea Infusions ◽

The World ◽

Brewing Time ◽

Ga Content ◽

Good Agreement

Abstract Black tea infusions are one of the most popular beverages across the world. Their extract composition depends on several factors, brewing time being one of the most important determinants. The aim of the present study was to determine the catechin composition of different black tea infusions using a validated LC electrospray ionization time-of-flight MS method. Additionally, total phenolic content (TPC) and antioxidant activity of infusions were evaluated using Folin–Ciocalteu reagent and stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). An optimized LC-MS method enabled the precise identification of the studied catechins [epicatechin (EC), EC gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG)] and gallic acid (GA). The major catechin in all investigated teas was EGC (25.6 mg/100 cm3 after 4 min of brewing). EC was present at the lowest concentration in all extracts. TPC and antiradical scavenging activity were in a good agreement with catechins and GA content. In general, the longer the brewing time, the higher the concentration of catechin, TPC, and antioxidant activity values. However, it should be noted that after 2 min brewing, most phenolics had already been extracted, and extract composition did not significantly change at a prolonged extraction time.

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Penentuan Kandungan Fenolik Total Liquid Volatile Matter dari Pirolisis Kulit Buah Kakao dan Uji Aktivitas Antifungi terhadap Fusarium oxysporum

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.15.1.24678.165-176 ◽

2019 ◽

Vol 15 (1) ◽

pp. 165 ◽

Cited By ~ 1

Author(s):

Mashuni Pallawagau ◽

Nur Arfa Yanti ◽

M. Jahiding ◽

La Ode Kadidae ◽

Wahyu Ahwarul Asis ◽

...

Keyword(s):

Antifungal Activity ◽

Fusarium Oxysporum ◽

Total Phenolic Content ◽

Phenolic Content ◽

Volatile Matter ◽

Total Phenolic ◽

Dilution Method ◽

Activity Test ◽

Cocoa Pod Husk

Kulit buah kakao (KBK) adalah limbah organik dari hasil olahan buah kakao yang dapat digunakan sebagai sumber senyawa aktif. Kulit kakao mengandung senyawa selulosa, hemiselulosa dan lignin yang dapat terdekomposisi menghasilkan senyawa fenolik. Penelitian ini bertujuan untuk menentukan kandungan fenolik total atau Total Phenolic Content (TPC) dari liquid volatile matter (LVM) hasil pirolisis KBK dan uji aktivitas antifungi terhadap Fusarium oxysporum. Produksi LVM dihasilkan dari metode pirolisis KBK pada suhu 500 °C. Penentuan TPC dengan metode spektrofotometri UV-Vis menggunakan pereaksi Folin- Ciocalteu (FC) pada panjang gelombang maksimum 765 nm dengan larutan standar asam galat dan diukur setelah dibiarkan selama 60 menit pada suhu kamar. Uji aktivitas antifungi terhadap F. oxysporum dilakukan dengan metode dilusi. LVM dari hasil pirolisis KBK adalah 25,80% (b/b) dengan TPC 100% sebesar 2,28 g/L. Pirolisis KBK menghasilkan LVM yang memperlihatkan aktivitas antifungi dengan konsentrasi hambat minimal (KHM) 1% (v/v) dan konsentrasi bunuh minimal (KBM) 9% (v/v). Oleh karena itu, LVM KBK memiliki potensi untuk diaplikasikan sebagai fungisida alami.Determination of The Total Phenolic Content of Liquid Volatile Matter from Cocoa Pod Husk Pyrolysis and Antifungal Activity Test Against Fusarium oxysporum. The cocoa pod husk (CPH) is an organic waste from the processed cocoa potentially used as source of active compounds. The CPH contains cellulose, hemicelluloses, and lignin compounds that can be decomposed into phenolic compounds. The present study investigated the determination of total phenolic content (TPC) of liquid volatile matter (LVM) of CPH followed by the antifungal activity test against Fusarium oxysporum. The LVM production was performed by the pyrolysis method at temperature of 500 °C. The determination of TPC was evaluated by the UV-Vis spectrophotometric method using the Folin-Ciocalteu (FC) reagent at maximum wavelength of 765 nm with the standart solution of gallic acid measured after 60 min reaction at room temperature. The antifungal activity test against F. oxysporum was carried out by dilution method. The pyrolysis of CPH produces LVM of 25.80% (b/b) contained TPC 100% of 2.28 g/L. The LVM produced in this study shows antifungal activity with a minimal inhibitory concentration (MIC) 1% (v/v) and minimum fungicidal concentration (MFC) 9% (v/v). Therefore, the LVM CPH produced can be applied as a natural fungicide.

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