scholarly journals Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

2016 ◽  
Vol 56 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Jahanshir Amini ◽  
Zahra Agapoor ◽  
Morahem Ashengroph
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Mycelial Growth ◽  
Biochemical Characterization ◽  
Culture Method ◽  
Biocontrol Agents ◽  
Dual Culture ◽  
Bacterial Strains ◽  
Chickpea Wilt

AbstractIn this study, about 112 isolates ofStreptomyceswere isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused byFusarium oxysporumf. sp.ciceris in vitrousing plate assay and selected for further studies. The selected strains were identified asStreptomycesspp. based on morphological and biochemical characterization as well as 16S rDNA sequences analysis. Our results assigned them to strains related to genus ofStreptomyces.In vitro, antagonistic effects ofStreptomycesstrains against the disease were evaluated through the dual-culture method, volatile and non-volatile metabolites, siderophore, protease and chitinase production. All bacterial strains inhibited mycelial growth of the pathogen ranging from 26 to 44.2% in dual culture assay. The non-volatile extract of five of theStreptomycesstrains inhibited more than 50% growth of the pathogen, whereas volatile compounds were less effective on mycelial growth inhibition (20.2 to 33.4%). The ability of the biocontrol agents to produce siderophore and protease were varied, whereas, production of chitinase was detected for all strains. Results of the greenhouse assay indicated that all biocontrol agents reduced disease severity (ranging from 38.7 to 54.8%). Accordingly, strain KS62 showed higher control efficacy (54.8%). In addition, the biomass of chickpea plants (plant height and dry weight) significantly increased in plants treated withStreptomycesstrains compared to non-bacterized control. The results of this study showed that it may be possible to manage chickpea Fusarium wilt disease effectively by usingStreptomycesspecies, as biocontrol agents. Therefore, evaluating their efficiency under field conditions is needed.

scholarly journals SCREENING OF PEAS (PISUM SATIVUM) VARIETIES/ LINES AGAINST FUSARIUM WILT (FUSARIUM OXYSPORUM .SP.PICI) AND IN VITRO EVALUATION OF FUNGICIDES AGAINST MYCELIAL GROWTH OF PATHOGEN

2019 ◽  
Vol 31 (1) ◽  
pp. 89-96
Author(s):  
Syed K. Husnain ◽  
Sabir H. Hussain ◽  
Muhammad Atiq ◽  
Nasir A. Rajput ◽  
Waseem Abbas ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Mycelial Growth ◽  
Plant Disease ◽  
Plant Mortality ◽  
Food Legumes ◽  
Pisum Sativum L ◽  
Chickpea Wilt

Twenty Peas (Pisum sativum L.) varieties/ lines were evaluated against Fusarium wilt caused by Fusarium oxysporum .Sp.pici by sowing them in sick plot during the year of 2016-17 at the Plant Pathology Research Institute, Faisalabad. Each cultivar/line was planted in a single row of three meter length, with plant to plant and row to row distances of 15cm and 30 cm respectively and replicated thrice by following Randomized Complete Block Design (RCBD). Out of these twenty varieties/ lines 13 including check variety Olympia were found highly susceptible ranging from 53.2 to 83.5% plant mortality. Six varieties/lines were susceptible ranging from 30.3 to 44.1 % plant mortality. Only a single variety Garrow performed as moderately resistant by showing 21% plant mortality in the field. Efficacy of five fungicides against Fusarium oxysporum .Sp.pici, at various concentrations was evaluated in-vitro and significant variations among treatments was observed. In general there was a significant decrease in mycelial growth of the fungus with an increase in concentration of fungicides. Tilt (Propiconazol),( Daconil (Chlorothalonil) and Crest (Carbendazim) were the most effective fungicides in inhibiting the growth of the fungus in descending order. The Tilt almost 90% inhibited the growth @ 50µg/ml concentration, Daconil and Crest exhibited intermediate effectiveness. Topsin-M (Thiophanate-methyl) and Score (Difenoconazole) were the least effective fungicides.Ahmad, M. A., S. M. Iqbal, N. Ayub, Y. Ahmad and A. Akram. 2010. Identification of resistant sources in chickpea against Fusarium wilt. Pak. J. Bot, 42: 417-426.Borum, D. E. and J. Sinclair. 1968. Evidence for systemic protection against Rhizoctonia solani with vitavax in cotton seedlings. Phytopathology, 58: 976-&.Davies, D., G. Berry, M. Heath and T. Dawkins. 1985. Pea (Pisum sativum L.). Grain Legume Crops. Collins, London, UK: 147-198.Food and Agriculture Organization of the United Nations. 2011Hagedorn, D. 1984. Compendium of pea diseases. 57 p. Am. Phytopathol. Soc., St. Paul, Minnesota, USA.Hannan, A., S. T. Sahi, I. Ahmed and A. A. Choudhry. 2014. Differential impact of Fusarium oxysporum f. sp. pisi on resistance source of pea genotypes and its chemical management. Pakistan Journal of Phytopathology, 26: 91-96.Haware, M. P. 1978. Eradication of Fusarium oxysporum f. sp. ciceri Transmitted in Chickpea Seed. Phytopathology, 68: 1364.Haware, M.P. and Nene, Y.L., 1982. Races of Fusarium oxysporum f.sp. ciceri. Plant disease, 66 (9), pp.809-810.Hulse, J. H. 1994. Nature, composition, and utilization of food legumes. Expanding the Production and Use of Cool Season Food Legumes. Springer Netherlands, pp. 77-97.Ilyas, M., M. Iqbal and K. Iftikhar. 1992. Evaluation of some fungicides against Fusarium oxysporum f. sp. ciceris and chickpea wilt. Pakistan Journal of  Phaytopahtology, 4: 5-8.Iqbal, S. M. 2005. Screening of chickpea genotypes for resistance against Fusarium wilt. Mycopath (Pakistan).Javaid, I. A., A. Ghafoorm and R. Anwar. 2002. Evaluation of local and exotic pea Pisum sativum germplasm for vegetable and dry grain straits. Pak. J. Bot, 34: 419-427.Khan, I., S. Alam and A. Jabbar. 2002. Selection for resistance to Fusarium wilt and its relationship with phenols in chickpea.Khan, S. A., A. Awais, N. Javed, K. Javaid, A. Moosa, I. U. Haq, N. A. Khan, M. U. Chattha and A. Safdar. 2016. Screening of pea germplasm against Fusarium oxysporum f. sp. pisi and invitro management through chemicals. Pakistan Journal of Phytopathology, 28: 127-131.Khokhar, M. 2014. Production status of major vegetables in Pakistan, their problems and suggestions. Agric. Corner, 9.Kraft, J. M. 1994. Fusarium wilt of peas (a review). Agronomie, 14: 561-567.Maitlo, S., R. Syed, M. Rustamani, R. Khuhro and A. Lodhi. 2014. Comparative efficacy of different fungicides against fusarium wilt of chickpea (Cicer arietinum L.). Pakistan Journal of Botany, 46: 2305-2312.McPhee, K. 2003. Dry pea production and breeding. Food, Agri Environ, 1: 64-69.Nawab, N. N., G. M. Subhani, K. Mahmood, Q. Shakil and A. Saeed. 2008. Genetic variability, correlation and path analysis studies in garden pea (Pisum sativum L.). J. Agric. Res, 46: 333-340.Nene, Y., M. Haware and M. Reddy. 1981. Chickpea diseases: resistance-screening techniques.Pande, S., J. N. Rao and M. Sharma. 2007. Establishment of the Chickpea Wilt Pathogen Fusarium oxysporum f. sp. ciceris in the Soil through Seed Transmission. The Plant Pathology Journal, 23: 3-6.Persson, L., L. Bødker and M. Larsson-Wikström. 1997. Prevalence and pathogenicity of foot and root rot pathogens of pea in Southern Scandinavia. Plant Disease, 81: 171-174.Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics, a biometrical approach. McGraw-Hill Kogakusha, Ltd.Sundar, A. R., N. Das and D. Krishnaveni. 1995. In-vitro antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian Journal of Plant Protection, 23: 152-155.Vyas, S. C. 1984. Systemic fungicides. Systemic fungicides.  

scholarly journals Exploration of The Biocontrol Potential of Halophilic Bacteria Against Sclerotium Oryzae and Rhizoctonia Solani

2021 ◽  
Author(s):  
Yalavarthi Nagaraju ◽  
Mahadeva swamy ◽  
R.C. Gundappagol ◽  
Nagaraj M Naik ◽  
K. Narayana Rao
Keyword(s):  
Plant Pathogens ◽  
Halophilic Bacteria ◽  
Hydrolytic Enzymes ◽  
Pseudomonas Stutzeri ◽  
Culture Method ◽  
Biocontrol Agents ◽  
Dual Culture ◽  
Bacterial Strains ◽  
16S Rrna Analysis

Abstract There is a pressing need for biocontrol agents to control soil borne plant pathogens through biocontrol agents. The focus of this research was on the interactions of halophilic bacterial isolates with the phytopathogenic fungi such as Sclerotium oryzae and R. solanii. The biocontrol ability of fifty bacterial strains isolated from saline soils of the Koppal (Gangavathi) and Raichur districts was assessed in vitro. Eight isolates, HB-10, HB-17, HB-28, HB-30, HB-39, HB-48, HB-49 and HB-50 showed strong antifungal activity against S. oryzae and R. solani in dual culture method. These prospective isolates were identified as Bacillus cereus (HB-48 and HB-49), Bacillus albus (HB-17), Bacillus safensis (HB-28), Staphylococcus xylosus (HB-39), Lysinibacillus sphaericus (HB-50) and Pseudomonas stutzeri (HB-10 and HB-30) based on 16S rRNA analysis. We found a link between isolates producing HCN, siderophore and hydrolytic enzymes and the biocontrol function of isolates. The isolate B. albus (HB-17), produced both catechol and hydroximate type of siderophores, confirmed in Arnow’s and Csaky’s tests. All isolates were tested for drug resistance and were shown to be immune to penicillin, oflaxacin and vancomycin. In addition, these isolates were investigated at three NaCl concentrations (3, 6 and 10 % w/v) for their plant growth promoting attributes viz., phosphorous, and zinc solubilization and potassium release. The solubilization zones of zinc carbonate, zinc oxide and phosphorous were within the range of 5.20 to 9.0 mm, 6.06 to 13.20 mm, 6.30 to 9.70 mm respectively, at 6 % NaCl concentration.

Construing the Role of Plant Extracts, Fungicides and Bio-agents in Ameliorating Fusarium Wilt Management in Chickpea

2021 ◽  
Author(s):  
Annie Khanna ◽  
Kushal Raj ◽  
Pankaj Kumar
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Seed Yield ◽  
Plant Extracts ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Field Conditions ◽  
Dual Culture ◽  
Chickpea Wilt

Background: Chickpea (Cicer arietinum L.) is the most important leguminous crop around the world. Fusarium wilt incited by Fusarium oxysporum f. sp. ciceris is a major biotic constraint in chickpea production. The present investigation was undertaken to evaluate the efficacy of plant extracts, fungicides and bio-agents against Fusarium oxysporum f. sp. ciceris under in vitro and field conditions. Methods: Plant extracts and fungicides were evaluated using poison food technique while antagonistic activity of bio-agents was studied using dual culture technique under in vitro conditions. Field trials were conducted to evaluate the efficacy of different plant extracts, fungicides and bio-agents against Fusarium wilt at Experimental Area of Plant Pathology, CCS HAU, Hisar.Result: Among thirteen plant extracts evaluated in vitro, neem leaves extract was found to be the most effective in inhibiting mycelial growth of F. oxysporum f. sp. ciceris followed by datura leaves’ and garlic cloves’ extract which were statistically at par. Out of six plant extracts tested against Fusarium wilt under field conditions, seed treatment with extracts of neem and datura leaves at 10% concentration were most effective and curtailed the wilt incidence by 39.02 and 34.14% along with 7.55 and 6.83% enhanced seed yield. Among fungicides, carbendazim 50 WP was the most toxic fungicide in restricting colony diameter of the pathogen with the least EC50 and EC90 values of 1.28 and 15.24 ppm a.i. followed by azoxystrobin 23 SC with corresponding values of 1.57 and 49.16 ppm a.i., respectively. Seed treatment with carbendazim 50 WP and azoxystrobin 23 SC were superior over other treatments and provided 88.41 and 85.98% reduction in disease incidence along with 12.85 and 10.99% higher seed yield over control. Among the bio-agents tested, T. viride and T. harzianum gave the best results in suppressing the pathogen growth in vitro and in minimizing the disease incidence coupled with improvement in seed yield under field conditions. The present study has provided chemical and non-chemical measures for integrated management of chickpea wilt.

scholarly journals Role of Biocontrol Agents in Management of Corm Rot of Saffron Caused by Fusarium oxysporum

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1398
Author(s):  
Vishal Gupta ◽  
Krishna Kumar ◽  
Kausar Fatima ◽  
Vijay Kumar Razdan ◽  
Bhagwati Charan Sharma ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Growth Promotion ◽  
Disease Incidence ◽  
Biocontrol Agents ◽  
Crocus Sativus ◽  
First Year ◽  
Dual Culture ◽  
Maximum Reduction

Saffron (Crocus sativus L.) is considered as one of the most expensive spices. Fusarium corm rot of saffron, caused by Fusarium oxysporum, is known to cause severe yield losses worldwide. In the present study, efficacy of biocontrol agents (Trichoderma asperellum, Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas putida, Bacillus stratosphericus, Bacillus pumilus, and Bacillus subtilis) along with a chemical fungicide, carbendazim, was evaluated for managing the corm rot of saffron. Under in vitro conditions, using dual culture and poison food techniques on potato dextrose agar, T. asperellum and carbendazim significantly reduced the mycelial growth of the pathogen F. oxysporum, with the inhibition of 62.76 and 60.27%, respectively, compared with control. Under field conditions, dipping of saffron corms in carbendazim and T. asperellum exhibited maximum reduction of 82.77 and 77.84%, respectively, in the disease incidence, during the first year of experiment. However, during the second year, maximum reduction in the incidence of corm rot (68.63%) was recorded with the T. asperellum. Moreover, the population density of F. oxysporum was also significantly reduced by 60 and 80.19% while using T. asperellum after 75 and 260 days of sowing of saffron corms, compared to its population before planting of corms. In case of growth promotion traits, such as sprouting and flowering, biocontrol treatments reduced the number of days (average) of sprouting and flower emergence after sowing, compared to control.

scholarly journals MEKANISME PARASITISME TRICHODERMA HARZIANUM TERHADAP FUSARIUM OXYSPORUM PADA SEMAI ACACIA MANGIUM

2015 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Susanti Tasik ◽  
Siti Muslimah Widyastuti ◽  
Harjono .
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Acacia Mangium ◽  
Culture Method ◽  
Inhibition Mechanism ◽  
Dual Culture ◽  
In Planta ◽  
Know How ◽  
Soil Borne Fungi

Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%).

scholarly journals Antagonisme Fungi Endofit Daun Jarak Pagar (Jatropha curcas) terhadap Fusarium oxysporum C2 Penyebab Busuk Umbi pada Bawang Merah in Vitro

2020 ◽  
Vol 4 (1) ◽  
pp. 47
Author(s):  
Fauziyyah Nahdah ◽  
Noorkomala Sari ◽  
Akhmad Rizali ◽  
Rabiatul Wahdah
Keyword(s):  
Fusarium Oxysporum ◽  
Jatropha Curcas ◽  
Linear Trend ◽  
Culture Method ◽  
Fungal Endophyte ◽  
Dual Culture ◽  
Antimicrobial Substances ◽  
Spss Software

<p class="Abstract">Basal plate rot is a major disease on shallot caused by <em>Fusarium oxysporum</em>. Endophytic fungi is promising to use as antagonist agent to the pathogen. Endophyte is microbes that are living in plant cells and have an asymptomatic characteristic. Nowadays, fungal endophyte is believed to produce antimicrobial substances similar with their plant host's natural product. <em>Jatropha curcas</em> is one of the plants containing secondary metabolites that have antifungal activities. The research aimed to study the ability of endophyte from <em>Jatropha curcas</em> to inhibit the growth of <em>Fusarium oxysporum</em>. The dual culture method was used in this research and the data were analyzed by SPSS software. This antagonism test was conducted by 9 isolates endophyte and each plate consisted of 3 replicates. The result revealed endophyte fungal obtaining 9 isolates with the radial growth of 4,5 cm/2 days. Endophytes of <em>Jatropha curcas</em> L. were able to inhibit the growth of <em>Fusarium oxysporum</em> C2. The percentage of inhibition of <em>Fusarium oxysporum </em>causing of root blight diseases was controlled by up 38.27 - 74.48%. The highest percentage of inhibition is gained by B4b and the lowest of it is A2b. Our observations showed that each endophyte has a consistent linear trend. B4b still leaded as the highest strength to inhibit the growth of pathogen on the monitoring of 3, 5, and 7 days. Moreover, the ability of fungi endophyte from <em>Jatropha curcas</em> as antagonist agent to <em>Fusarium oxysporum</em> needs to be further examined by the in vivo method.</p>

scholarly journals IN VITRO EFFICACY OF PGPR PSEUDOMONAS FLUORESCENS AGAINST FUSARIUM WILT OF TOMATO CAUSED BY FUSARIUM OXYSPORUM F.SP. LYCOPERSICI

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
K. Vignesh ◽  
K. Rajamohan ◽  
P. Balabaskar ◽  
R. Anandan
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Fusarium Wilt ◽  
Critical Role ◽  
Inhibition Zone ◽  
Antagonistic Effect ◽  
Culture Technique ◽  
Dual Culture ◽  
Percent Inhibition

Tomato (Solanum lycopersicum L.) is one of the most important, commercial and widely grown vegetable crop in the world. Tomato plays a critical role in nutritional food requirements, income and employment opportunities for the people. However, its production is threatened by the Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici and production losses between 30%to40%. In the present investigation an attempt has been made to study the in vitro efficacy of Pseudomonas fluorescens against Fusarium oxysporum f.sp. lycopersici. The antagonistic effect of Pseudomonas fluorescens were observed by the Dual culture technique and Agarwell method under the in vitro conditions.Among the ten isolates of Pseudomonas fluorescens, isolate Pf5 found to show the maximum percent inhibition over control (58.75%) and least mycelial growth (37.12mm) in dual culture technique against Fusarium oxysporum f.sp. lycopersici. In Agar well method isolate Pf5 proved out the maximum inhibition zone (17.47mm)against Fusarium oxysporumf.sp. lycopersici and percent inhibition over control (80.97%) at 30% concentration level.

scholarly journals Eco Friendly Management of Fusarium Wilt of Chickpea with Botanicals and Bio Agents in In-vitro

2020 ◽  
pp. 192-196
Author(s):  
K. L. Nandeesha ◽  
Shalini N. Huilgol ◽  
Geeta D. Goudar
Keyword(s):  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
Total Yield ◽  
Infected Plant ◽  
Cassia Tora ◽  
Cicer Arietinum L ◽  
Cent Concentration ◽  
Chickpea Wilt ◽  
Environment Condition

Chickpea (Cicer arietinum L.) is one of the most important pulse crop grown all over India. Chickpea wilt caused by Fusarium oxysporum f. sp. ciceri is one of the major disease on chickpea in Northern Karnataka, which is soil and seed borne. Heavy inoculum in the soil and favorable environment condition results in the death of infected plant and therefore total yield loss.In this study, three antagonists, and seven botanicals were studied against Fusarium oxysporum f. sp. ciceri causing chickpea wilt. In vitro studies found that among the botanicals, turmeric rhizome extract gave maximum per cent inhibition of mycelial growth (26.73%) and least per cent inhibition of mycelial growth (9.96%) was observed in cassia tora at 15 per cent concentration. Among the antagonists, Trichoderma harzianum was effective in per cent inhibition of Fusarium oxysporum f. sp. ciceri with (76.47%) and Pseudomonas fluorescens found least effective in per cent mycelial inhibition with (34.41%).

scholarly journals In vitro antifungal activity of endophytic bacteria isolated from date palm (Phoenix doctylifera L.) against fungal pathogens causing leaf spot of date palm

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
H. H. Al-Nadabi ◽  
N. S. Al-Buraiki ◽  
A. A. Al-Nabhani ◽  
S. N. Maharachchikumbura ◽  
R. Velazhahan ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Endophytic Bacteria ◽  
Mycelial Growth ◽  
Fungal Pathogens ◽  
Date Palm ◽  
Dual Culture ◽  
Bacterial Endophytes ◽  
Bacterial Strains ◽  
Culture Filtrates

Abstract Background Date palm ((Phoenix doctylifera L.) suffers from several fungal diseases. The endophytic microorganisms present in higher plants generally offer protection to their host plants against invading phytopathogenic fungi and bacteria. In the present study, endophytic bacteria associated with date palm leaves were isolated and their in vitro antagonistic potential against fungal pathogens causing leaf spots in date palm was demonstrated. Results Endophytic bacteria were isolated from date palm leaves of 3 different cultivars viz., Nighal, Khalas and Khinaizi and evaluated for their inhibitory activity against leaf spot pathogens of date palm viz., Fusarium solani, Alternaria sp., Nigrospora sp., Thieloviopsis sp., Curvularia subpapendrofii and Tilletiopsis minor using an in vitro dual culture assay. Of the 24 endophytic bacterial strains tested, the endophytes designated B1, B7, B8 and B9 obtained from cv. Nighal showed inhibitory activities (more than 55% mycelial growth inhibition) against F. solani and Alternaria sp. None of the bacterial endophytes inhibited the growth of other fungal pathogens tested. These antagonistic bacterial strains were identified as Pantoea septica on the basis of 16S rRNA gene sequence analysis. The hyphae of F. solani and Alternaria sp. exhibited morphological abnormalities such as shrinkage and disintegration when grown in the presence of antagonistic bacterial endophytes. The cell-free culture filtrates of the bacterial endophytes caused inhibition of mycelial growth and induced leakage of electrolytes from the mycelia of F. solani and Alternaria sp. This is the first study that describes inhibition of the date palm pathogens F. solani and Alternaria sp. by P. septica. Conclusion Endophytic Pantoea septica strains isolated from date palm leaves inhibited the mycelial growth of F. solani and Alternaria sp. and induced morphological changes in their mycelia. The culture filtrates of these bacterial strains also inhibited the mycelial growth and caused leakage of electrolytes from the mycelia of F. solani and Alternaria sp. These promising bacterial strains can be exploited as biocontrol agents to control F. solani and Alternaria sp.-induced leaf spot diseases of date palm.

scholarly journals ANTAGONISTIC TEST OF RIAU LOCAL FUNGAL ISOLATES AGAINST SOME PATHOGENIC IN CULTIVATED PLANTS

2019 ◽  
Vol 12 (2) ◽  
pp. 124-132
Author(s):  
Nia Safitri ◽  
Atria Martina ◽  
Rodesia Mustika Roza
Keyword(s):  
Fusarium Oxysporum ◽  
Crop Production ◽  
Plant Pathogens ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Cultivated Plants ◽  
Dual Culture ◽  
Trichoderma Sp

Tanaman budi daya merupakan tanaman yang sering diserang oleh cendawan pathogen, sehingga mengakibatkan penurunan populasi dan produksi tanaman. Pengendalian hayati dengan cendawan antagonis merupakan salah satu metode yang paling efektif dan lebih ramah lingkungan dalam menekan pertumbuhan patogen tanaman. Penelitian ini bertujuan untuk menguji aktivitas antagonis cendawan isolat lokal Riau terhadap beberapa cendawan patogen pada tanaman budi daya. Uji antagonis dilakukan secara in vitro dengan metode dual culture menggunakan lima belas cendawan isolat lokal Riau terhadap Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus dan Colletotrichum sansevieria. Hasil penelitian menunjukkan bahwa Trichoderma sp. PNE 4 memiliki aktivitas antagonis tertinggi dan isolat FER C1 serta isolat LLB07 hanya memiliki aktivitas antagonis yang tinggi dalam menekan pertumbuhan cendawan patogen. Trichoderma sp. PNE 4 mampu menghambat pertumbuhan miselium F. oxysporum sebesar 85,30%, G. Philippii (100%), G. boninense (100%), dan C. sansevieria (100%). Isolat FER C1 hanya menghambat R. Microporus (50,39%) dan isolat LLB07 menghambat G. philippii (52,20%). Trichoderma sp. PNE 4 merupakan cendawan uji yang terpilih sebagai cendawan antagonis, karena memiliki kemampuan daya hambat  >70%.Abstract Cultivated plants are often attacked by pathogenic fungi resulting in a decline of population and crop production. Biocontrol with antagonistic fungi is one of the most effective and environmentally friendly methods in suppressing the growth of plant pathogens. This study aims to examine the antagonistic activity of local isolates fungi Riau against some pathogenic fungi on cultivated plants. The antagonistic test was performed in vitro by dual culture method using fifteen local isolates fungal Riau against Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus and Colletotrichum sansevieria. The results showed that Trichoderma sp. PNE 4 isolate exhibited highest activites and  FER C1 and LLB07 isolates exhibited high activities suppressed the growth of the fungal pathogen. Trichoderma sp. PNE 4 isolate inhibited mycelial growth F. oxysporum (85.30%), G. philippii (100%), G. boninense (100%) and C. sansevieria (100%). FER C1 isolate only inhibited R. microporus (50.39%), and LLB07 isolate inhibited G. philippii (52.20%). Trichoderma sp. PNE 4 isolate is test isolates as fungal antagonistic.

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