scholarly journals Fluorescein as a diagnostic marker of bladder ruptures: an experimental study on rabbit model

2016 ◽  
Vol 60 (2) ◽  
pp. 213-217
Author(s):  
Özgür Aksoy ◽  
Başak Kurt ◽  
Celal Şahin Ermutlu ◽  
Kürşat Çeçen ◽  
Sadık Yayla ◽  
...  
Keyword(s):  
Experimental Study ◽  
Fluid Flow ◽  
New Zealand ◽  
Diagnostic Marker ◽  
Abdominal Cavity ◽  
Rabbit Model ◽  
Intestinal Content ◽  
Bladder Rupture ◽  
Fluorescein Dye ◽  
New Zealand Rabbits

Abstract Introduction: The aim of this study was to investigate fluorescein use in the diagnosis of bladder ruptures in rabbits as an experimental model. Material and Methods: The study was conducted on male New Zealand rabbits divided into a retrograde fluorescein group (n = 8) and an intravenous (IV) fluorescein group (n = 8). Following general anaesthesia, 10 mL of 10% fluorescein dye (sodium fluoresceine powder) was administered via ureterorenoscope to the bladder of the first group, and 0.5 mL of 10% fluorescein was administered intravenously to the second group. Then, the bladder was viewed through the cystoscope by urethral aspect. After experimental bladder perforation, groups were comparatively evaluated by paracentesis and laparotomy. Results: Following IV injection of fluorescein dye, the bladder veins were stained green within 10 s and then fluorescein mixed with urine flowed into bladder lumen. The green fluid flow was observed in the abdominal cavity after the perforation of the bladder in both groups. Conclusion: Fluorescein can be used as a marker in diagnosis of bladder ruptures. If there is no bleeding or intestinal content in the abdominal cavity, although a smoky yellow-green image is observed, bladder rupture can be suspected.

scholarly journals Rabbit Model of Retinoblastoma

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Shin Jeong Kang ◽  
Hans E. Grossniklaus
Keyword(s):  
Drug Delivery ◽  
New Zealand ◽  
Blood Vessels ◽  
Rabbit Model ◽  
Fundus Images ◽  
Retinoblastoma Cells ◽  
Pathologic Features ◽  
Human Retinoblastoma ◽  
New Zealand Rabbits ◽  
Subretinal Injection

We created a rabbit model of retinoblastoma and confirmed the tumor clinically and histopathologically. Seventeen New Zealand rabbits were immunosuppressed with cyclosporin A at doses of 10–15 mg/kg. At day 3, the animals received a 30 μl subretinal injection of cultured WERI retinoblastoma cells. Digital fundus images were captured before euthanasia, and the eyes were submitted for histopathology. Retinoblastoma cells grew in all the inoculated eyes and established a tumor under the retina and/or in the vitreous. New blood vessels in the tumor were observed starting at week 5. Cuffs of viable tumor cells surrounded the blood vessels with regions of necrosis present at 70–80μm from nutrient vessels. Occasional tumor seeds in the vitreous histologically exhibited central necrosis. This rabbit model demonstrated similar fundus appearance and pathologic features to human retinoblastoma and may be used as a model to test various routes of drug delivery for retinoblastoma.

scholarly journals Remote Subcutaneous Needling to Suppress the Irritability of Myofascial Trigger Spots: An Experimental Study in Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Zhonghua Fu ◽  
Yueh-Ling Hsieh ◽  
Chang-Zern Hong ◽  
Mu-Jung Kao ◽  
Jaung-Geng Lin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Experimental Study ◽  
New Zealand ◽  
Random Sampling ◽  
Rabbit Skeletal Muscle ◽  
Degree Of Reduction ◽  
Electrophysiological Effects ◽  
New Zealand Rabbits

Objective. To obtain electrophysiological effects of Fu’s subcutaneous needling (FSN) on needling distance by assessment of endplate noise (EPN) recorded from the myofascial trigger spots (MTrSs) in rabbit skeletal muscle.Method. Eighteen New Zealand rabbits weighing 2.5–3.0 kg were randomly divided into two groups as follows: proximal needling (PN) group and distal needling (DN) group. The needling procedure followed the instructions described by the inventor of FSN, including needling insertion and swaying movement. The amplitudes of EPN on the MTrS region of BF muscle were recorded as an index of MTrS irritability. Random sampling of EPN tracings were taken for further analyses before, during, and after FSN treatment.Results. In PN and DN groups, the trends of EPN amplitude alterations were similar at conditions before, during, and after FSN treatment. The degree of reduction in the EPN amplitude in PN group was significantly higher than that in DN group. There were no significant changes in EPN amplitudes in the MTrS of contralateral BF without FSN intervention either in DN or PN group.Conclusion. The irritability of proximal MTrSs could be modulated after ipsilateral FSNs. The placement of FSN may affect the effectiveness of suppression of irritability of MTrSs.

scholarly journals Effects of omega 3 on doxorubicin-induced cardiotoxicity in an experimental rabbit model

2021 ◽  
Vol 10 (13) ◽  
pp. e60101320993
Author(s):  
Jéssica Cristina de Barros ◽  
Amanda Garcia Pereira ◽  
Marcela Aldrovani Rodrigues ◽  
Daniel Paulino Junior
Keyword(s):  
Single Dose ◽  
New Zealand ◽  
Healthy Adult ◽  
Rabbit Model ◽  
Volume Density ◽  
Omega 3 ◽  
Transverse Area ◽  
Acute Cardiotoxicity ◽  
Different Doses ◽  
New Zealand Rabbits

To evaluate the effects of the use of omega 3 at different doses, following the response through stereological and morphometric evaluations in doxorubicin-induced acute cardiotoxicity in an experimental rabbit model. Thirty healthy adult New Zealand rabbits were divided into six groups with different doses of omega 3 treatment, GT125, GT500 and GT 2000, receiving doses of 125mg / kg, 500mg / kg and 2000mg / kg, respectively. The induction of acute cardiotoxicity with doxorubicin was treated for six days prior to intravenous administration of 10mg / kg single dose. Then, the animals were euthanized and myocardial samples were collected for stereological and morphometric evaluations. The stereological evaluation showed an increase in the transverse area of ​​cardiomocytes between the SHAM and GT2000 groups and an increase in the interstitial collagen volume density between the SHAM and GT500 and SHAM and GT2000 groups. The omega 3 500mg/kg dose has the potential to attenuate the fibrotic effects of doxorubicin use and the use of a single dose of 10mg/kg doxorubicin can be used as an acute dilated cardiomyopathy induction protocol in an experimental model using rabbits.

Evaluation of the Extent of Vessel Avulsion Injury: An Experimental Study Using a Rabbit Model

2018 ◽  
Vol 35 (04) ◽  
pp. 294-298
Author(s):  
Muhammet Uraloğlu ◽  
Alper Ural ◽  
İsmail Saygın ◽  
Murat Livaoğlu ◽  
Naci Karaçal
Keyword(s):  
Experimental Study ◽  
Rabbit Model ◽  
T Test ◽  
Vascular Endothelial ◽  
Avulsion Injury ◽  
The Mean ◽  
The Difference ◽  
Extent Of Damage ◽  
Paired T Test ◽  
New Zealand Rabbits

Background Determining the extent of damage in vascular avulsion type injuries still represents a challenge for the microsurgeon. Excision of the damaged section is critical for the success of anastomosis. The purpose of this study was to determine which among vascular endothelial and adventitia damage findings is most effective in determining the extent of avulsion injury. Methods Varying degrees of avulsion were applied to the aorta of 12 (n = 12) adult female New Zealand rabbits. Avulsion was first determined using adventitial findings and then with endothelial findings. The definitive extent of damage was determined histopathologically. Results The mean area of the histopathological sections was 16.7 ± 6.9 mm. The extent of damage measured from the adventitia was 15.8 ± 7.9 mm (the difference in histopathological examinations was not significant, p = 0.590, paired t-test), while the extent of damage from the lumen was 13.3 ± 8.2 mm (the difference in histopathological examinations was significant p = 0.004, paired t-test). Conclusion Excision based on adventitia findings is more effective in case of avulsion injury.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

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