scholarly journals Analysis of cooperative interactions in molecularly imprinted polymer nanoparticles

2015 ◽  
Vol 3 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Jatin Mistry ◽  
Antonio Guerreiro ◽  
Ewa Moczko ◽  
Elena Piletska ◽  
Kal Karim ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Fold Increase ◽  
Solid Support ◽  
Cooperative Binding ◽  
Support Surface ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Cooperative Interactions ◽  
High Affinity Binding Site ◽  
Number Of Binding Sites

AbstractCooperative binding is commonly observed in biological receptor systems. This study investigates whether it is possible to prepare nano-sized molecularly imprinted polymers (nanoMIPs) that show cooperative binding. NanoMIPs which exhibit cooperative binding would have increased affinity for immobilised template molecules making them useful for advanced applications in diagnostics and sensors. The use of a templatederivatised solid support provides a facile route to prepare nanoMIPs with surface imprints, and the method is ideally suited to study this topic. Although not observed during the course of this study, positive interbinding site cooperativity was hypothesised by way of an increase in the number of binding sites imprinted on the nanoMIPs, by increasing template density on the solid support surface. After synthesis, the affinity of nanoMIPs was analysed using surface plasmon resonance (SPR) technique. Under the conditions investigated, a ten fold increase in binding affinity was measured as template density was increased. SPR results could be explained by an increase in cooperative binding; however calculations showed that the increase in affinity was not significant enough to prove cooperative binding interactions. The main conclusion obtained was that MIP nanoparticles contain only one “high-affinity” binding site that interacts with immobilised template in an SPR assay.

Application progress of magnetic molecularly imprinted polymer chemical sensors in the detection of biomarkers

The Analyst ◽  
2022 ◽  
Author(s):  
Ying Wang ◽  
Xiaomin Yang ◽  
Lin Pang ◽  
Pengfei Geng ◽  
Fang Mi ◽  
...  
Keyword(s):  
Chemical Sensors ◽  
Molecularly Imprinted Polymers ◽  
Essential Role ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Magnetic Molecularly Imprinted Polymer ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Highly Sensitive ◽  
Detection Of Biomarkers ◽  
Highly Sensitive Detection

Specific recognition and highly sensitive detection of biomarkers play an essential role in identifying, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIP) have been widely used...

Preparation of carbon-based metal organic frameworks modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

2022 ◽  
Author(s):  
Xue Chen ◽  
Jinyue Chai ◽  
Baodong Sun ◽  
Xue Yang ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Metal Organic Framework ◽  
Selective Recognition ◽  
Template Molecule ◽  
Bovine Hemoglobin ◽  
Carbon Spheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Metal Organic ◽  
Carbon Based

In this study, the carbon-based Cu2+-immobilized metal-organic framework modified molecularly imprinted polymer (C@GI@Cu-MOFs@MIPs) adsorbent was prepared using bovine hemoglobin (BHb) as a template molecule with carbon spheres as carriers for...

Magnetic molecularly imprinted polymers–silver nanoparticle based micro-solid phase extraction for the determination of polycyclic aromatic hydrocarbons in water samples

RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54702-54708 ◽  
Author(s):  
Mostafa Khajeh ◽  
Marzieh Sharifirad ◽  
Mousa Bohlooli ◽  
Mansour Ghaffari-Moghaddam
Keyword(s):  
Silver Nanoparticle ◽  
Aromatic Hydrocarbons ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Magnetic Molecularly Imprinted Polymer ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Polycyclic Aromatic

In this study, an efficient and sensitive magnetic molecularly imprinted polymer–silver nanoparticle (MMIPS) system was successfully synthesized.

Recent developments in molecularly imprinted polymer nanofibers and their applications

2015 ◽  
Vol 7 (18) ◽  
pp. 7406-7415 ◽  
Author(s):  
Shabi Abbas Zaidi
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Molecularly Imprinted Polymers ◽  
Imprinted Polymer ◽  
Polymer Nanofibers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Recent Developments ◽  
Analyte Detection

Molecularly imprinted polymers (MIPs) have been potential and versatile candidates for analyte detection.

Simple and selective detection of quercetin in extracts of plants and food samples by dispersive-micro-solid phase extraction based on core–shell magnetic molecularly imprinted polymers

2018 ◽  
Vol 42 (19) ◽  
pp. 16144-16153 ◽  
Author(s):  
Arash Asfaram ◽  
Maryam Arabi ◽  
Abbas Ostovan ◽  
Hossein Sadeghi ◽  
Mehrorang Ghaedi
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Food Samples ◽  
Selective Detection ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymer ◽  
Magnetic Molecularly Imprinted Polymers

In the present study, a D-μ-SPE clean-up method was established for the analysis of quercetin in extracts of plants and food samples using a magnetic molecularly imprinted polymer as the sorbent by HPLC-UV detection.

scholarly journals Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers

2005 ◽  
Vol 30 (4) ◽  
pp. 67-73 ◽  
Author(s):  
Chin-Yin Hung ◽  
Han-Hung Huang ◽  
Ching-Chiang Hwang
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Mandelic Acid ◽  
Occupational Exposures ◽  
Bulk Polymerization ◽  
Chiral Separations ◽  
Phenyl Acetic Acid ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Chromatographic Resolution

Styrene is used in a variety of chemical industries. Environmental and occupational exposures to styrene occur predominantly through inhalation. The major metabolite of styrene is present in two enantiomeric forms, chiral R- and S- hydroxy-1-phenyl-acetic acid (R-and S-mandelic acid, MA). Thus, the concentration of MA, particularly of its enantiomers, has been used in urine tests to determine whether workers have been exposed to styrene. This study describes a method of analyzing mandelic acid using molecular imprinting techniques and HPLC detection to perform the separation of diastereoisomers of mandelic acid. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting using (+) MA, (-) MA or (+) phenylalanine, (-) phenylalanine as templates. Methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were copolymerized in the presence of the template molecules. The bulk polymerization was carried out at 4ºC under UV radiation. The resulting MIP was grounded into 25~44¼m particles, which were slurry packed into analytical columns. After the template molecules were removed, the MIP-packed columns were found to be effective for the chromatographic resolution of (±)-mandelic acid. This method is simpler and more convenient than other chromatographic methods.

Cholesterol-Imprinted Receptor Using Chitosan Derivative as the Precursor

2013 ◽  
Vol 699 ◽  
pp. 712-717
Author(s):  
Huai Min Guan ◽  
Yue Jin Tong ◽  
Jin Hua Zheng ◽  
Xiang Li
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Polar Solvent ◽  
Binding Capacity ◽  
Template Molecule ◽  
Chitosan Derivative ◽  
Amino Groups ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Protection ◽  
Selection Of

Research has been focused on the development of molecularly imprinted polymers using a chitosan derivative as the precursor. An O-acyl chitosan was synthesized by the selective protection of amino groups of chitosan in MeSO3H and was cross-linked with glutaraldehyde in the presence/ absence of template molecule, cholesterol. The effect of the degree of the acyl substitution on the selection of precursor was investigated, regarding the solubility of chitosan derivative, interaction between the precursor and imprinted molecule, and degree of the cross-linking of precursor. The rebinding experiments indicated the significant recognition for cholesterol with imprinted polymer as compared with non-imprinted polymer. It was found that a good binding capacity of the imprinted polymer towards cholesterol could be achieved in a less-polar solvent. And the O-acyl chitosan-based molecularly imprinted polymer obtained displayed good recognition selectivity for cholesterol in comparison to similarly strctural analogue, cholesterol acetate.

A chemiluminescence sensor for determination of lysozyme using magnetic graphene oxide multi-walled carbon nanotube surface molecularly imprinted polymers

RSC Advances ◽  
2016 ◽  
Vol 6 (15) ◽  
pp. 12391-12397 ◽  
Author(s):  
Yanhui Wang ◽  
Huimin Duan ◽  
Leilei Li ◽  
Xiaojiao Wang ◽  
Jianbo Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Molecularly Imprinted Polymers ◽  
High Selectivity ◽  
Imprinted Polymer ◽  
Magnetic Graphene Oxide ◽  
Molecularly Imprinted ◽  
Magnetic Graphene ◽  
Multi Walled Carbon Nanotube

In this paper, a new chemiluminescence sensor possessing high selectivity and sensitivity was established for determination of lysozyme using magnetic graphene oxide–multi-walled carbon nanotube surface molecularly imprinted polymer.

scholarly journals Phthalocyanine-Based Molecularly Imprinted Polymers as Nucleoside Receptors

2008 ◽  
Vol 2008 ◽  
pp. 1-5 ◽  
Author(s):  
Luigia Longo ◽  
Giuseppe Vasapollo
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Capacity ◽  
Zinc Phthalocyanine ◽  
Scatchard Analysis ◽  
Binding Affinities ◽  
Imprinted Polymer ◽  
Binding Ability ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Combined Use

A molecularly imprinted polymer (MIP) for tri-O-acetyladenosine (TOAA), PPM(TOAA), was prepared by the combined use of methacrylic acid (MAA) and Zn(II)tetra(4'-methacryloxyphenoxy) phthalocyanine as functional monomers. This MIP exhibited a higher binding ability for TOAA compared to the MIP prepared using only MAA, PM(TOAA), in batch rebinding tests. Scatchard analysis gave a higher association constant of PPM(TOAA) for TOAA (2.96×104 M−1) than that of PM(TOAA) (1.48×104 M−1). The MIP prepared using only the zinc-phthalocyanine, PP(TOAA), did not show any binding capacity for TOAA. This means that the phthalocyanine in the MIP contributes to higher affinities, although it barely interacts with TOAA. Since selectivity for this kind of MIPs is more important than binding affinity, the binding of TOAA and a structurally related compound, tri-O-acetyluridine (TOAU), on the polymers was investigated. Both PPM(TOAA) and PM(TOAA) exhibited binding affinities for TOAA while they did not show any binding capacity for TOAU.

scholarly journals Recent Advances in Electrosynthesized Molecularly Imprinted Polymer Sensing Platforms for Bioanalyte Detection

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1204 ◽  
Author(s):  
Robert Crapnell ◽  
Alexander Hudson ◽  
Christopher Foster ◽  
Kasper Eersels ◽  
Bart Grinsven ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Biologically Relevant ◽  
Sensing Applications ◽  
Sensing Platforms ◽  
Target Analytes ◽  
Constant Potential ◽  
Number Of Publications ◽  
Potential Methods

The accurate detection of biological materials has remained at the forefront of scientific research for decades. This includes the detection of molecules, proteins, and bacteria. Biomimetic sensors look to replicate the sensitive and selective mechanisms that are found in biological systems and incorporate these properties into functional sensing platforms. Molecularly imprinted polymers (MIPs) are synthetic receptors that can form high affinity binding sites complementary to the specific analyte of interest. They utilise the shape, size, and functionality to produce sensitive and selective recognition of target analytes. One route of synthesizing MIPs is through electropolymerization, utilising predominantly constant potential methods or cyclic voltammetry. This methodology allows for the formation of a polymer directly onto the surface of a transducer. The thickness, morphology, and topography of the films can be manipulated specifically for each template. Recently, numerous reviews have been published in the production and sensing applications of MIPs; however, there are few reports on the use of electrosynthesized MIPs (eMIPs). The number of publications and citations utilising eMIPs is increasing each year, with a review produced on the topic in 2012. This review will primarily focus on advancements from 2012 in the use of eMIPs in sensing platforms for the detection of biologically relevant materials, including the development of increased polymer layer dimensions for whole bacteria detection and the use of mixed monomer compositions to increase selectivity toward analytes.

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