Prevalence and antimicrobial resistance of Arcobacter butzleri and Arcobacter cryaerophilus isolates from retail meat in Lower Silesia region, Poland

2015 ◽  
Vol 18 (1) ◽  
pp. 63-69 ◽  
Author(s):  
I. Zacharow ◽  
J. Bystroń ◽  
E. Wałecka-Zacharska ◽  
M. Podkowik ◽  
J. Bania
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Chicken Meat ◽  
Significant Incidence ◽  
Arcobacter Butzleri ◽  
Contaminated Food ◽  
Retail Meat ◽  
Human Poisoning ◽  
Meat Samples ◽  
Lower Silesia

AbstractArcobacter butzleriandA. cryaerophilusare considered potential foodborne pathogens. Consumption ofArcobacter-contaminated food is regarded the most likely source of human poisoning. We investigated the prevalence and antimicrobial resistance ofArcobacterisolates in 210 retail meat samples. Seventy-nineA. butzleriand 6A. cryaerophiluswere isolated from pork, beef and chicken meat. Incidence ofA. butzleriwas found to be the highest in chicken meat (83%). Less ofA. butzleriwas isolated from beef (16%) and pork (14%). Most of theA. butzleriisolates were resistant to β-lactams, like ampicillin (85%), amoxicillin with clavulonic acid (63%), cefotaxime (66%) and macrolides, i.e., erythromycin (62%). In contrast, all except oneA. cryaerophilusisolates were susceptible to erythromycin. Tetracycline and aminoglycosides showed the highest efficacy againstA. butzleriandA. cryaerophilussince almost 80% of their population was susceptible to these agents. All, except oneA. cryaerophilusand the majority ofA. butzleriisolates (70%) were susceptible to fluoroquinolones. The incidence of multiresistant isolates was found in forty two (53%)A. butzleri, and one (16%)A. cryaerophilusisolates. EightA. butzleriisolates were resistant to all antimicrobials tested. These results indicate significant incidence of potential foodborne zoonotic agents, i.e.A. butzleriandA. cryaerophilusincluding multiresistant isolates in retail meat in Poland.

scholarly journals Integrated surveillance for antimicrobial resistance in Salmonella from clinical and retail meat sources reveals genetically related isolates harboring quinolone and ceftriaxone resistant determinants

2021 ◽  
Author(s):  
Nkuchia M M’ikanatha ◽  
Xin Yin ◽  
Sameh W Boktor ◽  
Lisa A Dettinger ◽  
Deepanker Tewari
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Antimicrobial Agents ◽  
Significant Proportion ◽  
Resistance Mechanisms ◽  
Health Concern ◽  
Fluoroquinolone Resistance ◽  
Drug Classes ◽  
Retail Meat ◽  
Meat Samples

Abstract Background Antimicrobial resistance in foodborne pathogens, including non-typhoidal Salmonella (NTS), is a public health concern. Pennsylvania conducts integrated surveillance for antimicrobial resistance in NTS from human and animal sources. Methods During 2015-2017, clinical laboratories submitted 4,478 NTS isolates from humans and 96 isolates were found in 2,520 retail meat samples. One hundred and nine clinical isolates that shared pulsed-field gel electrophoresis patterns with meat isolates and all strains from meat samples were tested for susceptibility to antimicrobial agents. Six clinical and 96 NTS isolates from meat sources (total 102) were analyzed by whole-genome sequencing (WGS). Results 28 (25.7 %) of the 109 clinical NTS and 21 (21.9%) of strains from meat sources had resistance to ≥3 antimicrobial drug classes (MDR). Sixteen (15.7%) of the 102 isolates analyzed by WGS had resistance mechanisms that confer resistance to expanded-spectrum cephalosporins (ESCs), such as ceftriaxone. We identified a bla CTX-M-65 in two S. Infantis isolates from clinical and three S. Infantis isolates from meat sources. These five bla CTX-M-65 -positive S. Infantis strains carried ≥5 additional resistance genes plus a D87Y mutation in gyrA that encodes fluoroquinolone resistance. WGS showed that isolates from patients and meat samples were within ≤10 and ≤5 alleles for S. Infantis and S. Reading, respectively. Conclusions A significant proportion of NTS isolates from human and animal sources were MDR and 16% had genetic mechanisms that confer resistant to ceftriaxone. These results emphasize need for integrated surveillance in healthcare and agricultural settings.

scholarly journals Antimicrobial Susceptibility Pattern of Gram-Negative Bacterial Isolates from Raw Chicken Meat Samples

2019 ◽  
Vol 6 ◽  
pp. 89-95
Author(s):  
Neha Gautam ◽  
Rojan Poudel ◽  
Binod Lekhak ◽  
Milan Kumar Upreti
Keyword(s):  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Chicken Meat ◽  
Food Microbiology ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Kathmandu Valley ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Meat Samples

Objectives: This research aims to study the microbial quality of chicken meat available in retail shop of Kathmandu Valley. Methods:  This Study was conducted from June to December 2018 in three different districts of Kathmandu Valley. Samples were collected in sterile plastic bags, labeled properly and stored in an icebox and transported to the Food Microbiology laboratory of Golden Gate International College.  During sample preparation, 25 grams of each sample was taken and transferred to sterile flasks containing 225 ml of buffered peptone water. Potential pathogenic Gram-negative bacteria were isolated by using respective selective media and identified by biochemical test. Antibiotic susceptibility profile of isolates was carried out by Kirby-Bauer disc diffusion method according to CLSI 2017 guideline. Results: Of total 81 chicken meat samples processed; 201 Gram negative bacteria were isolated.  E. coli (100%) was the dominant Gram-negative isolates, followed by Citrobacter spp (62.96%), Pseudomonas spp (40.74%), Proteus spp (19.75%), Salmonella spp (16.04%) and Klebsiella spp (8.64%) respectively. No any multidrug isolates were detected. Conclusion: The study showed that the raw chicken meat samples of Kathmandu valley was highly contaminated with Gram negative potential pathogenic bacteria. Antimicrobial resistance pattern shown by the isolates may indicates that there is not overuse of drug in animals and the less chance of risk of increasing antimicrobial resistance.

scholarly journals Antimicrobial Resistance Pattern of Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 420 ◽  
Author(s):  
Mst. Sonia Parvin ◽  
Sudipta Talukder ◽  
Md. Yamin Ali ◽  
Emdadul Haque Chowdhury ◽  
Md. Tanvir Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Biochemical Properties ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Isolation And Identification ◽  
E Coli ◽  
Encoding Genes

Escherichia coli is known as one of the most important foodborne pathogens in humans, and contaminated chicken meat is an important source of foodborne infection with this bacterium. The occurrence of extended-spectrum β-lactamase (ESBL)-producing E. coli (ESBL-Ec), in particular, in chicken meat is considered a global health problem. This study aimed to determine the magnitude of E. coli, with special emphasis on ESBL-Ec, along with their phenotypic antimicrobial resistance pattern in frozen chicken meat. The study also focused on the determination of ESBL-encoding genes in E. coli. A total of 113 frozen chicken meat samples were purchased from 40 outlets of nine branded supershops in five megacities in Bangladesh. Isolation and identification of E. coli were done based on cultural and biochemical properties, as well as PCR assay. The resistance pattern was determined by the disc diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% of samples were positive for E. coli, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively, while only 11.6% were resistant to 3–5 classes. Possible extensive drug resistance (pXDR) was found in 2.3% of isolates. High single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim–sulfamethoxazole, and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for the blaTEM gene. However, the blaSHV and blaCTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates carried the blaCTX-M-1 gene. This study provided evidence of the existence of MDR and pXDR ESBL-Ec in frozen chicken meat in Bangladesh, which may pose a risk to human health if the meat is not properly cooked or pickled raw only. This emphasizes the importance of the implementation of good slaughtering and processing practices by the processors.

Prevalence, Antibiotic Resistance, and Molecular Characterization of Salmonella Serovars in Retail Meat Products

2011 ◽  
Vol 74 (1) ◽  
pp. 161-166 ◽  
Author(s):  
JI-YEON HYEON ◽  
JUNG-WHAN CHON ◽  
IN-GYUN HWANG ◽  
HYO-SUN KWAK ◽  
MOO-SANG KIM ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Molecular Subtypes ◽  
Meat Products ◽  
Chicken Meat ◽  
Source Tracking ◽  
Traditional Markets ◽  
Retail Meats ◽  
Retail Meat

The prevalence of Salmonella was determined in chicken meat (n = 26), beef (n = 49), and pork (n = 56) collected from wholesale markets, retail stores, and traditional markets in Seoul, South Korea, in 2009. Antibiotic resistance was assessed, and the molecular subtypes of Salmonella isolates were ascertained using an automated repetitive sequence–based PCR (rep-PCR) system (DiversiLab). A total of 18 Salmonella strains were isolated from 17 of 131 samples: 16 strains from each of 16 samples and 2 strains from the same pork sample. The prevalence of Salmonella from the retail meats was 2.0% in beef, 8.9% in pork, and 42.3% in chicken meat. Among 10 different serotypes, Salmonella enterica Panama was recovered from a beef sample, and Salmonella London and Salmonella Montevideo were the predominant serotypes from pork and chicken meat, respectively. The highest antibiotic resistance observed was to erythromycin (100%) followed by streptomycin (22.2%) and tetracycline and chloramphenicol (16.7%). Of the 18 isolates, 5 (27.8%) were resistant to two or more antibiotics, and 1 isolate from chicken meat was resistant to eight antibiotics, including cephalosporins. Differentiation between all of the Salmonella isolates except between Salmonella Montevideo and Salmonella London was successfully performed with the automated rep-PCR system, indicating that it can be added to the toolbox for source tracking of foodborne pathogens associated with outbreaks.

scholarly journals Prevalence and antimicrobial resistance of Salmonella serotypes isolated from retail chicken meat and giblets in Iran

2015 ◽  
Vol 9 (05) ◽  
pp. 463-469 ◽  
Author(s):  
Hamid Reza Sodagari ◽  
Zohreh Mashak ◽  
Amir Ghadimianazar
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Chicken Meat ◽  
Resistance Testing ◽  
Retail Markets ◽  
Predominant Type ◽  
Amoxicillin Clavulanic Acid ◽  
Resistant Strains ◽  
Resistance Rates ◽  
Retail Chicken Meat

Introduction: Salmonella is one of the major foodborne pathogens responsible for outbreaks of foodborne illness in humans worldwide. Methodology: A total of 560 samples of chicken meat and giblets were collected from retail markets for Salmonella identification, serotyping, and antimicrobial resistance testing. Results: Salmonella was detected in 19.8% of samples. Among the five serotypes identified, S. Thompson was the predominant type (48.7%). High antimicrobial resistance rates were observed to nalidixic acid (92.8%), tetracycline (81%), trimethoprim (68.4%), sulfamethoxazole / trimethoprim (61.2%), streptomycin (56.7%), and kanamycin (36.9%). Although resistance to chloramphenicol (3.6%), amoxicillin-clavulanic acid (5.4%), and ampicillin (11.7%) was detected, none of the isolates were resistant to ceftazidime, ceftriaxone, cefotaxime, ciprofloxacin, colistin, gentamicin, nor imipenem. Conclusions: Restrictions on the irrational use of antibiotics in humans and animals are suggested for the reduction of resistant strains.

Prevalence and Multidrug Resistance Pattern of Salmonella Carrying Extended-Spectrum β-Lactamase in Frozen Chicken Meat in Bangladesh

2020 ◽  
Vol 83 (12) ◽  
pp. 2107-2121
Author(s):  
MST. SONIA PARVIN ◽  
MD. MEHEDI HASAN ◽  
MD. YAMIN ALI ◽  
EMDADUL HAQUE CHOWDHURY ◽  
MD. TANVIR RAHMAN ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Policy Makers ◽  
Disk Diffusion Test ◽  
Extended Spectrum ◽  
Meat Samples

ABSTRACT Salmonella is an important foodborne pathogen that causes public health problems globally, and the increase of antimicrobial resistance in Salmonella has intensified the problem. Chicken meat is an important reservoir and disseminator of Salmonella to humans. This study aimed at estimating the burden of Salmonella carrying extended-spectrum β-lactamase (ESBL) and their antimicrobial resistance pattern in 113 domestic frozen chicken meat samples purchased from supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of β-lactamase–, and plasmid-mediated quinolone resistance–encoding genes. All samples were analyzed for the presence of Salmonella using selective media and PCR assay. Antimicrobial susceptibility test was done by disk diffusion test, and ESBL screening was performed by double-disk synergy tests. Resistance genes were detected using multiplex PCR. Of samples, 65.5% were positive for Salmonella spp., and, of these, 58.1% isolates were ESBL producers. All the isolates were multidrug resistant (MDR): 40.5% were resistant to both three to five and six to eight antimicrobial classes; 17.6% were resistant to 9 to 11 classes, and 1.4% isolates to 12 to 15 classes. The highest rates of resistance were observed against oxytetracycline (100%), followed by trimethoprim-sulfamethoxazole (89.2%), tetracycline (86.5%), nalidixic acid (83.8%), amoxicillin (74.3%), and pefloxacin (70.3%). Notably, 48.6% of isolates demonstrated resistance to imipenem. One (1.4%) isolate was possibly extensively drug resistant. All the isolates were positive for the blaTEM gene, 2.7% were positive for blaCTX-M-1, and 20.3% for blaNDM-1. The prevalence of qnrA and qnrS genes was 4.1 and 6.8%, respectively. This study shows that ESBL-producing Salmonella are widespread in frozen chicken meat in Bangladesh, which puts greater responsibility on food processors and policy makers to ensure food safety. HIGHLIGHTS

scholarly journals Antimicrobial Resistance of Campylobacter Isolates from Retail Meat in the United States between 2002 and 2007

2010 ◽  
Vol 76 (24) ◽  
pp. 7949-7956 ◽  
Author(s):  
S. Zhao ◽  
S. R. Young ◽  
E. Tong ◽  
J. W. Abbott ◽  
N. Womack ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Relatedness ◽  
Ground Beef ◽  
Sampling Period ◽  
The United States ◽  
Health Concern ◽  
Chicken Breast ◽  
Contamination Rate ◽  
Retail Meat ◽  
Meat Samples

ABSTRACT The emergence of antimicrobial resistance in Campylobacter spp. has been a growing public health concern globally. The objectives of this study were to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Campylobacter spp. recovered by the National Antimicrobial Resistance Monitoring System (NARMS) retail meat program. Retail meat samples (n = 24,566) from 10 U.S. states collected between 2002 and 2007, consisting of 6,138 chicken breast, 6,109 ground turkey, 6,171 ground beef, and 6,148 pork chop samples, were analyzed. A total of 2,258 Campylobacter jejuni, 925 Campylobacter coli, and 7 Campylobacter lari isolates were identified. Chicken breast samples showed the highest contamination rate (49.9%), followed by ground turkey (1.6%), whereas both pork chops and ground beef had <0.5% contamination. The most common resistance was to doxycycline/tetracycline (46.6%), followed by nalidixic acid (18.5%), ciprofloxacin (17.4%), azithromycin and erythromycin (2.8%), telithromycin (2.4%), clindamycin (2.2%), and gentamicin (<0.1%). In a subset of isolates tested, no resistance to meropenem and florfenicol was seen. C. coli isolates showed higher resistance rates to antimicrobials, with the exception of doxycycline/tetracycline, than those seen for C. jejuni. Pulsed-field gel electrophoresis (PFGE) fingerprinting resulted in 1,226 PFGE profiles among the 2,318 isolates, with many clones being widely dispersed throughout the 6-year sampling period.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

scholarly journals Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

2020 ◽  
Author(s):  
Thang Nam Nguyen ◽  
Diep Thi Khong ◽  
Ha Viet Le ◽  
Hoa Thi Tran ◽  
Quang Ngoc Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Contamination ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Meat Sample ◽  
E Coli ◽  
Drug Resistant Bacteria ◽  
The Status ◽  
Retail Meat ◽  
Meat Samples

Abstract The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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