scholarly journals Feed Restriction and Muscle Fibre Characteristics of Pectoralis Major in Broiler Chickens

2017 ◽  
Vol 48 (1) ◽  
pp. 8-12
Author(s):  
D. Chodová ◽  
E. Tůmová
Keyword(s):  
Muscle Fibre ◽  
Broiler Chickens ◽  
Pectoralis Major ◽  
Feed Restriction ◽  
Muscle Fibres ◽  
Cross Sectional ◽  
Fibre Area ◽  
Histological Characteristics ◽  
Musculus Pectoralis Major ◽  
Fibre Characteristics

Abstract The aim of the study was to evaluate the effect of one-week quantitative feed restriction (in days 8-14 of age) on histological characteristics of musculus pectoralis major in broiler chickens during the fattening period. Cockerels of Ross 308 (1215 chicken) were divided into 3 groups: ad libitum fed (AL; 3 × 135), restricted 80% of AL (R80; 3 × 135), and with limited feed intake 65% of AL (R65; 3 × 135). Eight cockerels per group were slaughtered in weekly intervals from day 14 of age till the end of experiment at 35 days to determine changes in the muscle fibre characteristics. The number of muscle fibres in pectoralis major decreased (P ≤ 0.001) with increasing age. Fibre cross sectional area was s ignificantly (P ≤ 0.027) affected by the interaction of group and age. At the end of feed restriction at 14 days all groups had similar fibre area (519-539 μm2), the differences between groups were observed at 35 days of age with the largest fibre area in R65 chickens (2296 μm2), while R80 did not differ from AL (1728 μm2 and 1667 μm2). There was no effect of feeding regime on giant muscle fibre incidence.

scholarly journals Effect of synbiotic dietary supplementation on histological and histopathological parameters of Pectoralis major muscle of broiler chickens

2018 ◽  
Vol 63 (No. 7) ◽  
pp. 263-271 ◽  
Author(s):  
J. Bogucka ◽  
D.M. Ribeiro ◽  
R.P.R. Costa ◽  
M. Bednarczyk
Keyword(s):  
Muscle Fibre ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Fibre Diameter ◽  
Pectoralis Major ◽  
Muscle Fibre Types ◽  
Control Group ◽  
Muscle Fibres ◽  
Histopathological Changes ◽  
The Impact

Bioactive substances may constitute an alternative to antibiotics. Probiotics are mixtures of microorganisms that enhance the effectiveness and yield of nutrient absorption by competing for the substrate against pathogens that may cause intestinal infections. Prebiotics are organic substances which include complex carbohydrates and have an influence on the growth and activity of desirable intestinal microflora. Synbiotics are a combination of both of them. The aim of this study was to analyze the impact of synbiotics added to commercial feed on Pectoralis major muscle microstructure in broiler chickens. Research material consisted of 240 one-day-old Ross 308 female chicks. Birds were divided into 2 groups, 12 broilers each (replicated 10 times). The control group (C) was fed a commercial diet, and the SYN group was fed the same diet with added synbiotic: 0.8% of prebiotic RFO (raffinose family oligosaccharides) extracted from lupin seeds and 1% of probiotic Lavipan<sup>®</sup>. Birds were slaughtered at day 42. Immediately after slaughter, samples of the Pectoralis major muscle for histological analysis were taken and frozen in liquid nitrogen. The staining procedures performed were: hematoxylin and eosin staining to evaluate fibre diameter and histopathological changes, oil red staining to determine intramuscular fat content, NADH-TR (tetrazolium reductase) activity to distinguish muscle fibre types and alkaline phosphatase reaction for counting the number of capillaries. The tendency to reduced muscle fibre diameter in chickens supplemented with synbiotics indicates an increase in muscle fibre density, which gives meat a more delicate structure. When assessing the occurrence of histopathological changes, significantly more fibre splitting was observed in the control group. Moreover, the positive effect of feed supplementation with synbiotics on muscle microvascularization could have an important practical meaning, because low physical activity of chickens during rearing may lead to ischemic muscles, increased changes in the structure of muscle fibres, and reduction of meat quality.

scholarly journals SLCV–a supervised learning—computer vision combined strategy for automated muscle fibre detection in cross-sectional images

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7053
Author(s):  
Anika Rettig ◽  
Tobias Haase ◽  
Alexandr Pletnyov ◽  
Benjamin Kohl ◽  
Wolfgang Ertel ◽  
...  
Keyword(s):  
Computer Vision ◽  
Supervised Learning ◽  
Muscle Fibre ◽  
Cross Sections ◽  
Reconstruction Error ◽  
Muscle Fibres ◽  
Dice Similarity Coefficient ◽  
Cross Sectional ◽  
Staining Methods ◽  
Combined Strategy

Muscle fibre cross-sectional area (CSA) is an important biomedical measure used to determine the structural composition of skeletal muscle, and it is relevant for tackling research questions in many different fields of research. To date, time consuming and tedious manual delineation of muscle fibres is often used to determine the CSA. Few methods are able to automatically detect muscle fibres in muscle fibre cross-sections to quantify CSA due to challenges posed by variation of brightness and noise in the staining images. In this paper, we introduce the supervised learning-computer vision combined pipeline (SLCV), a robust semi-automatic pipeline for muscle fibre detection, which combines supervised learning (SL) with computer vision (CV). SLCV is adaptable to different staining methods and is quickly and intuitively tunable by the user. We are the first to perform an error analysis with respect to cell count and area, based on which we compare SLCV to the best purely CV-based pipeline in order to identify the contribution of SL and CV steps to muscle fibre detection. Our results obtained on 27 fluorescence-stained cross-sectional images of varying staining quality suggest that combining SL and CV performs significantly better than both SL-based and CV-based methods with regards to both the cell separation- and the area reconstruction error. Furthermore, applying SLCV to our test set images yielded fibre detection results of very high quality, with average sensitivity values of 0.93 or higher on different cluster sizes and an average Dice similarity coefficient of 0.9778.

Comparisons of meat quality and muscle fibre characteristics on multiple pig breeds and sexes using principal component analysis

2018 ◽  
Vol 58 (11) ◽  
pp. 2091 ◽  
Author(s):  
Jun-Mo Kim ◽  
Sang-Hoon Lee ◽  
Youn-Chul Ryu
Keyword(s):  
Muscle Fibre ◽  
Meat Quality ◽  
Principal Component ◽  
Muscle Fibres ◽  
Drip Loss ◽  
Quality Traits ◽  
Pig Breeds ◽  
Meat Quality Traits ◽  
Fibre Characteristics

Comprehensive understanding of the effect of pig breed and sex on meat quality and post-mortem muscle conditions will be necessary to estimate meat quality post mortem. In this study, we applied multiple regression and principal component analysis (PCA) using conventional meat quality traits and muscle fibre characteristics on six pig breeds and different sexes. A total of 1374 pigs from six breeds [Berkshire, Duroc, Landrace, Meishan, Yorkshire, and LYD (Landrace × Yorkshire sows with Duroc as terminal sires) crossbreed] belonging to three sexes (male, castrated male and female) were used in this study. Most of the muscle fibre characteristics showed moderate to high significant correlations with conventional meat quality traits. In particularly, the cross-sectional area (CSAF) of muscle fibres was strongly correlated with muscle pH values, water-holding capacity values and lightness (0.10 < |r| <0.26 and P < 0.01). Multiple regression analysis by general linear model revealed that the effects of breed were significant on muscle pH24h, lightness, drip loss, and marbling score (P < 0.01), whereas sex had no significant effect on meat quality. In the muscle fibre characteristics, the effects of breed and sex were significant on composition of area and density of muscle fibres (P < 0.05). We observed that the Meishan breed was clearly inferior to other breeds, whereas the LYD or Berkshire breeds showed better meat quality and muscle fibre properties. Moreover, we observed clear separations between pig breeds based on the PCA, whereas the sexes in each breed presented a continuum cluster. In summary, we suggest that muscle pH24h, lightness, and drip loss are conventional meat quality traits that can be considered as quantitative factors important in estimating ultimate meat quality of pigs along with the CSAF of muscle fibres; we conclude that Meishan pigs have low meat quality, whereas Berkshire and LYD crossbred pigs have higher quality of pork.

The Effect of Knee Injury on the Number of Muscle Fibres in the Human Quadriceps Femoris

1982 ◽  
Vol 62 (2) ◽  
pp. 227-234 ◽  
Author(s):  
A. Young ◽  
I. Hughes ◽  
J. M. Round ◽  
R. H. T. Edwards
Keyword(s):  
Knee Injury ◽  
Quadriceps Muscle ◽  
Cross Sectional Area ◽  
Thigh Muscle ◽  
Muscle Fibres ◽  
Sectional Area ◽  
Cross Sectional ◽  
Fibre Area ◽  
The Cross ◽  
Fibre Atrophy

1. By means of ultrasound scanning, bilateral measurements of the cross-sectional area of the quadriceps muscle group were made in 14 young adults with unilateral thigh muscle wasting after knee injury. Needle biopsy specimens from the lateral mass of the muscle were used to estimate the myofibre cross-sectional area for both quadriceps of each subject. 2. The cross-sectional area of the quadriceps of each patient's injured limb was always smaller than that of the contralateral muscle. The wasting was largely localized to the quadriceps, with relative sparing of the other thigh muscles. 3. None of the biopsies showed any abnormality apart from a reduction in fibre size. In each case, the injured limb's reduced quadriceps cross-sectional area was associated with a reduced mean fibre area. 4. The ratio of the cross-sectional area of a muscle to its mean fibre area is a function of the number of fibres it contains. The ratio varied considerably from patient to patient but there was close agreement between the values obtained for the two limbs of each patient. 5. The quadriceps wasting produced by knee injury was due to muscle fibre atrophy. There was no evidence for a change in the number of fibres in the muscle.

The effects of age at slaughter, genotype and finishing system on the biochemical properties, muscle fibre type characteristics and eating quality of bull beef from suckled calves

1998 ◽  
Vol 66 (2) ◽  
pp. 341-348 ◽  
Author(s):  
C. A. Maltin ◽  
K. D. Sinclair ◽  
P. D. Warriss ◽  
C. M. Grant ◽  
A. D. Porter ◽  
...  
Keyword(s):  
Muscle Fibre ◽  
Fibre Type ◽  
Biochemical Properties ◽  
Cross Sectional Area ◽  
Muscle Fibres ◽  
Sectional Area ◽  
Cross Sectional ◽  
Percentage Area ◽  
Slow Twitch ◽  
Intramuscular Fat Content

AbstractMuscle fibre characteristics and biochemical properties of muscle recovered from young bulls of two genotypes (Aberdeen Angus × and Charolais ×), reared on two different diets (silage-based and barley-based) and slaughtered at varying ages between 10 and 19 months of age were established. These analyses were restricted to samples ofm. longissimus lumborum (LI) recovered at 48 h post mortem, vacuum packed and stored at 2°C for 14 days. Biochemical measurements included intramuscular fat content, intramuscular collagen content and its solubility, haem pigment concentration, sarcomere length and myofibril fragmentation. Muscle fibre type was classified according to the contractile nature of thefibres and their metabolic properties.Intramuscular fat content increased (P < 0·01) with age at slaughter and at a fixed age was greater for Angus × than Charolais × bulls (211·5 v. 295·8 mg/g dry matter, P < 0·01). Total intramuscular collagen and its solubility tended to decrease with age (P < 0·01). Differences in haem pigment concentration in samples of LI were detected between genotype (3·99 v. 3·59 mg/g for Angus × and Charolais × bulls; P × 0·01) and diet (3·97 v. 3·62 mg/g for bulls given barley and silage; P < 0·01), and increased with age at slaughter (P < 0·01). There was a significant increase in eye muscle cross-sectional area with increasing slaughter date (P < 0·01) and this was paralleled by an increase in cross-sectional area of individual muscle fibres (P < 0·001). Differences in cross-sectional area of individual muscle fibres between genotype and diet were small and inconsistent. Charolais × bulls had a greater percentage area of fast twitch glycolytic fibres than Angus × bulls (54·3 v. 49·3%; P < 0·01) and a smaller percentage area of slow twitch oxidative fibres (15·8 v. 18·9%; P < 0·05). Beef tenderness was positively correlated (r = 0·48; P < 0·01) with the frequency of slow twitch oxidative fibres and negatively correlated (r = -0·38; P < 0·05) with the frequency offast twitch glycolytic fibres.

Local differences in myotendinous junctions in axial muscle fibres of carp (Cyprinus carpio L.)

1996 ◽  
Vol 199 (4) ◽  
pp. 825-833
Author(s):  
I L Y Spierts ◽  
H A Asker ◽  
I H C Voss ◽  
J W M Osse
Keyword(s):  
Cyprinus Carpio ◽  
Myotendinous Junction ◽  
Muscle Fibres ◽  
Cross Sectional ◽  
Stress Load ◽  
Fibre Area ◽  
Axial Muscle ◽  
Carp Cyprinus Carpio ◽  
The Difference ◽  
Myotendinous Junctions

We studied the myotendinous junctions of anterior and posterior red and white axial muscle fibres of carp using stereology. In posterior axial muscle fibres of swimming fish, stress (load on the myotendinous junction) must be higher than in anterior fibres as posterior fibres have a longer phase of eccentric activity. As we expected the magnitude of the load on the junction to be reflected in its structure, we compared the interfacial ratio, the ratio between the area of the junctional sarcolemma and the cross-sectional fibre area, of these muscle fibres. This ratio differed significantly between the investigated groups, with red fibres and posterior fibres having the larger ratios. The higher interfacial ratio of posterior myotendinous junctions is in accordance with the proposition mentioned above. The difference between myotendinous junctions of red and white fibres is probably related to a difference in the duration of the load on the junction.

scholarly journals Effect of housing system and genotype on rabbit meat quality

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 190-199 ◽  
Author(s):  
D. Chodová ◽  
E. Tůmová ◽  
M. Martinec ◽  
Z. Bízková ◽  
V. Skřivanová ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Muscle Fibre ◽  
Meat Quality ◽  
Housing System ◽  
Muscle Fibres ◽  
Physical Parameters ◽  
Type I ◽  
Guard Hair ◽  
Lower Weight ◽  
Fibre Characteristics

The effect of the housing system on the carcass characteristics, physical parameters of meat quality, fatty acid composition, and muscle fibre characteristics was studied in some Czech breeds. Ninety-six rabbits from seven different breeds of Czech genetic resources (Moravian Blue, Czech White, Czech Solver, Czech Spotted, Moravian White of Brown Eye, Czech Gold, and Czech Black Guard Hair) and one rabbit commercial hybrid (Hyplus), kept in two housing systems: intensive system (wire-net cages) or alternative (straw-bedded pen), were slaughtered at the age of 91 days. Alternatively housed rabbits had lower weight at slaughter, lower weight of loin, of hind legs meat, and of renal fat than rabbits from cages. The interactions between housing system and genotype were reflected significantly in pH value, and lightness and yellowness of biceps femoris. The highest (P &le; 0.047) pH was observed in Hyplus (6.68) from cages, while the lowest value was noted in Moravian White of Brown Eye (6.26). The significantly (P &le; 0.010) lightest meat was detected in Czech Solver (60.93) and the darkest in Czech Gold (47.81). Alternatively reared rabbits showed significantly (P &le; 0.001) lower monounsaturated fatty acids (MUFA) (26.63%) and higher (P &le; 0.001) polyunsaturated fatty acids (PUFA) (36.73%) contents than rabbits from cages (36.94% MUFA and 26.23% PUFA). The alternatively housed group had also higher n-3 and n-6 PUFA contents and higher PUFA : SFA ratio than the intensively housed one. Significant interactions (P &le; 0.001) were observed in cross sectional area (CSA), diameter, and perimeter of muscle fibres of type I. The largest (P &le; 0.001) CSA of type I muscle fibre had Czech Black Guard Hair from cages (2573.1&nbsp;&mu;m<sup>2</sup>), while in pens this breed exhibited the smallest CSA (1219.6 &mu;m<sup>2</sup>), diameter (38.68 &mu;m), and perimeter (130.2 &mu;m). Fibre type distribution was not affected by any of the monitored parameters. The effect of interactions of the housing system and genotype was manifested mainly in physical and muscle fibre characteristics. &nbsp;

scholarly journals Composition and cross-sectional area of muscle fibre types in relation to daily gain and lean and fat content of carcass in Landrace and Yorkshire pigs

1996 ◽  
Vol 5 (6) ◽  
pp. 593-600 ◽  
Author(s):  
Marita Ruusunen ◽  
Marja-Liisa Sevon-Aimonen ◽  
Eero Puolanne
Keyword(s):  
Muscle Fibre ◽  
Live Weight ◽  
Carcass Composition ◽  
Cross Sectional Area ◽  
Muscle Fibre Types ◽  
Muscle Fibres ◽  
Type I ◽  
Sectional Area ◽  
Cross Sectional ◽  
Yorkshire Pigs

The muscle fibre-type properties of longissimus were compared between Landrace and Yorkshire breeds and between the sexes in an attempt to shed light on the relationship of these histochemical parameters to animal growth and carcass composition. Muscle fibres were classified into three groups, type I, type lIA and type 11B, using the myosin ATPase method. At a given live weight, the cross-sectional area of type I fibres (CSA1) was smaller (p

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