Neuroprotective effects of the Chrysophyllum perpulchrum extract against an Alzheimer-like rat model of β amyloid1-40 intrahippocampal injection

Abstract Objective Alzheimer’s disease (AD) is a threatening disease for African populations in the upcoming years because of the increase in their expectancy of life. Here, we investigated whether natural products from Chrysophyllum perpulchrum as catechin and two dimeric procyanidins (catechin + hexose) could prevent progression of oxidative stress and cognitive changes using an AD-like rat model induced by Aβ1-40 injection into the hippocampal CA1 subfield. Methodology Adult male Wistar rats were either microinjected with 1% ammonia as a vehicle (10 µL) or aggregated Aβ1-40 at 10 µg bilateral hippocampus. On the 14th day of post-surgery, some Aβ rats were treated with melatonin (10 mg/kg i.p.) or with the Chrysophyllum perpulchrum extract (300 mg/kg p.o.), and some sham-operated rats received the extract alone. Cognitive abilities were tested with Y-maze, object recognition test and Morris Water Maze. Oxidative stress markers as well as the level of activated microglial cells were assayed in the brain. Results Aβ rats exhibited significant deficits of recognition memory and spatial learning. This was associated with an increase of microglia Iba 1 immunoreactivity as well as nitric oxide (NO), malondialdehyde and superoxide dismutase levels but not to the thiol content in the hippocampus, prefrontal cortex and septum of AD-like rats. The Chrysophyllum perpulchrum extract treatment mitigated Aβ-induced cognitive impairments and reversed microglia overactivation and subsequent generation of oxidative stress markers. Interestingly, the neuroprotective actions of the Chrysophyllum perpulchrum extract seem to be comparable to the control drug melatonin used albeit with some more beneficial effects. Conclusion These findings are preliminary and should be strengthened by more pharmacological studies of bioactive compounds of Chrysophyllum perpulchrum before being proposed as a promising drug against AD.

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Glycyrrhizic acid ameliorates submandibular gland oxidative stress, autophagy and vascular dysfunction in rat model of type 1 diabetes

Scientific Reports ◽

10.1038/s41598-021-04594-w ◽

2022 ◽

Vol 12 (1) ◽

Author(s):

Saad Mohamed Asseri ◽

Nehal M. Elsherbiny ◽

Mohamed El-Sherbiny ◽

Iman O. Sherif ◽

Alsamman M. Alsamman ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Model ◽

Glycyrrhizic Acid ◽

Type I Diabetes ◽

Vascular Dysfunction ◽

Type I ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Ameliorative Effect ◽

Related Proteins

AbstractThe burden of diabetes mellitus (DM) and associated complications is increasing worldwide, affecting many organ functionalities including submandibular glands (SMG). The present study aims to investigate the potential ameliorative effect of glycyrrhizic acid (GA) on diabetes-induced SMG damage. Experimental evaluation of GA treatment was conducted on a rat model of type I diabetes. Animals were assigned to three groups; control, diabetic and GA treated diabetic groups. After 8 weeks, the SMG was processed for assessment of oxidative stress markers, autophagy related proteins; LC3, Beclin-1 and P62, vascular regulator ET-1, aquaporins (AQPs 1.4 and 5), SIRT1 protein expressions in addition to LC3 and AQP5 mRNA expressions. Also, parenchymal structures of the SMG were examined. GA alleviated the diabetes-induced SMG damage via restoring the SMG levels of oxidative stress markers and ET-1 almost near to the normal levels most probably via regulation of SIRT1, AQPs and accordingly LC-3, P62 and Beclin-1levels. GA could be a promising candidate for the treatment of diabetes-induced SMG damage via regulating oxidative stress, autophagy and angiogenesis.

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The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals

Scientific Reports ◽

10.1038/s41598-020-72098-0 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Mluleki Luvuno ◽

Andile Khathi ◽

Musa V. Mabandla

Keyword(s):

Oxidative Stress ◽

Cognitive Function ◽

Glucose Tolerance ◽

Interleukin 1Β ◽

Oral Glucose ◽

Regular Exercise ◽

Oxidative Stress Markers ◽

Object Recognition Test ◽

Dopamine Concentration ◽

Stress Markers

Abstract Changes associated with cognitive function in the high-fat high-carbohydrate diet-induced prediabetes animal model and effect of exercise remain unclear. Rats were randomly assigned to the following groups (n = 6): non-diabetic (ND), prediabetic (PD), intermittent exercising PD (PD + IE) and regular exercising PD (PD + RE). After exercise cessation, oral glucose tolerance (OGT), Novel Object Recognition Test (NORT) and Morris-Water Maze (MWM) tests were performed to assess cognitive function. After sacrifice, malonaldehyde, glutathione peroxidase, interleukin-1β and dopamine concentration in the prefrontal cortex (PFC) and hippocampus were measured. Impaired OGT response in PD animals was accompanied by poor performance on behavioural tasks. This was associated with increased oxidative stress markers and impaired dopamine neurotransmission as evidence by elevated dopamine concentration in the PFC and hippocampal tissue. Improved OGT response by exercise was coupled with improved performance on behavioural tasks, oxidative stress markers and increased interleukin-1β concentration. In regular exercise, this was further coupled with improved dopamine neurotransmission. Cognitive function was affected during prediabetes in animals. This was partly due to oxidative stress and impaired dopamine neurotransmission. Both intermittent and regular exercise improved cognitive function. This was partly mediated by improved glucose tolerance and oxidative stress as well as a subclinical increase in interleukin-1β concentration. In regular exercise, this was further mediated by improved dopamine neurotransmission.

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The relationship of oxidative stress markers and parameters of myocardial function in a rat model of cardiotoxicity

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2014.10.794 ◽

2014 ◽

Vol 75 ◽

pp. S42 ◽

Cited By ~ 1

Author(s):

Premysl Mladenka ◽

Tomáš Filipský ◽

Michal Ríha ◽

Jaroslava Vávrová ◽

Magdalena Holecková ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Model ◽

Myocardial Function ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Relationship Of ◽

The Relationship

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Multi-organ Toxicity Attenuation by Cerium Oxide and Yttrium Oxide Nanoparticles: Comparing the Beneficial Effects on Tissues Oxidative Damage Induced by Sub-acute Exposure to Diazinon

Pharmaceutical Nanotechnology ◽

10.2174/2211738508666200808135226 ◽

2020 ◽

Vol 8 (3) ◽

pp. 225-238

Author(s):

Mona Navaei-Nigjeh ◽

Marzieh Daniali ◽

Mahban Rahimifard ◽

Mohammad R. Khaksar

Keyword(s):

Oxidative Stress ◽

Cerium Oxide ◽

Metal Oxide Nanoparticles ◽

Radical Scavenging ◽

Oxide Nanoparticles ◽

Oxidative Stress Markers ◽

Beneficial Effects ◽

Stress Markers ◽

Kidney Liver ◽

The Brain

Background: Excessive use of diazinon, as an organophosphate pesticide (OP), contributes to cytotoxic and pathologic cellular damage and, in particular, oxidative stress. However, metal-oxide nanoparticles (NPs), such as cerium oxide (CeO2) and yttrium oxide (Y2O3), with the property of free radical scavenging demonstrated beneficial effects in the alleviation of oxidative stress biomarkers. Objective: The aims of this study include evaluating beneficial effects of CeO2 NPs, Y2O3 NPs, and their combination against diazinon-induced oxidative stress in different tissues of brain, heart, lung, kidney, liver, and spleen. Methods: Eight randomized groups of 6 adult male Wistar rats were formed. Each group of rats administered a different combination of diazinon, CeO2 and Y2O3 NPs daily and levels of oxidative stress markers, such as reactive oxygen species (ROS), lipid peroxidation (LPO), total thiol molecules (TTM) and total anti-oxidant power (TAP) and catalase enzyme, were measured after 2 weeks of the treatment. Results: Measurements of the mentioned markers in the brain, heart, lung, kidney, liver, and spleen showed that the administration of NPs could significantly alleviate the oxidative stress induced by diazinon. However, the findings of this study illustrated that the combination of both CeO2 and Y2O3 NPs led to a better reduction in oxidative stress markers. Conclusion: Sub-acute exposure of diazinon in rats led to increased levels of oxidative stress markers in pivotal tissues such as the brain, heart, lung, kidney, liver, and spleen. CeO2 and Y2O3 NPs neutralize the oxidative stress to compensate diazinon-induced tissue damages. Lay Summary: Organophosphate pesticides (OPs), which are mainly used for pest control, are responsible for the entry of pesticides into the human food cycle. Organophosphate such as diazinon increases the molecular biomarkers of oxidative stress inside the cells of vital tissues such as the heart, liver, lungs, etc. Metal oxide nanoparticles (NPs) such as cerium oxide (CeO2) and yitrium oxide (Y2O3) can have free radical scavenging potential under oxidative stress and through various mechanisms. Although these nanoparticles reduce oxidative stress, it should be borne in the design of the study that additional doses of these substances reverse the beneficial effects.

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Early Changes in Oxidative Stress Markers in a Rat Model of Acute Stress: Effect of l-carnitine on the Striatum

Basic & Clinical Pharmacology & Toxicology ◽

10.1111/j.1742-7843.2011.00691.x ◽

2011 ◽

Vol 109 (2) ◽

pp. 123-129 ◽

Cited By ~ 18

Author(s):

Luis A. Méndez-Cuesta ◽

Berenice Márquez-Valadez ◽

Verónica Pérez-De la Cruz ◽

Perla D. Maldonado ◽

Ricardo A. Santana ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Model ◽

Acute Stress ◽

Stress Effect ◽

Oxidative Stress Markers ◽

Stress Markers

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Spironolactone and Dimethylsulfoxide Effect on Glucose Metabolism and Oxidative Stress Markers in Polycystic Ovarian Syndrome Rat Model

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0033-1363685 ◽

2014 ◽

Vol 122 (03) ◽

pp. 154-162 ◽

Cited By ~ 9

Author(s):

A. Dăneasă ◽

C. Cucolaș ◽

M. Furcea ◽

P. Bolfa ◽

S. Dudea ◽

...

Keyword(s):

Oxidative Stress ◽

Glucose Metabolism ◽

Rat Model ◽

Polycystic Ovarian Syndrome ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress ◽

Ovarian Syndrome

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Effect of complete and partial capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.20.405 ◽

2019 ◽

Vol 20 (4) ◽

pp. 29-36

Author(s):

Yomna Khater ◽

Awad Rizk ◽

Mohamed Saad ◽

Adel Zaghloul

Keyword(s):

Oxidative Stress ◽

Renal Function ◽

Serum Creatinine ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Post Surgery ◽

Renal Function Tests ◽

And Oxidative Stress

Objective: To compare the effect of complete and partial renal capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury. Design: Randomized controlled experimental study. Animals: A 60 Spraque-dawely rats weighing 180 ± 50 g. Procedures: Rats were divided into 3 groups in triplicate (6 each). In addition, 6 rats were subjected to blood and renal tissues sampling for estimation of normal parameters. Group 1 (Positive control): ischemia reperfusion (IR) injury; Group 2: Complete capsulotomy + I R; Group 3: Partial capsulotomy + IR. Six rats from each group were sacrified at 2, 7 and 14 days post- surgery. Results: The complete capsulotomy induced a significant decrease in the serum creatinine at 2 and 7 days post- capsulotomy in comparison with partial capsulotomy (P < 0.05), whereas at 14 days, the partial capsulotomy induced the significant decrease (P < 0.05). Complete capsulotomy showed a significant improvement in creatinine clearance in comparasion with partial capsulotomy at 2, 7 and 14 days post- surgery (p<0.05). At 2 and 7 days, BUN of IR+ Capsulotomy group showed a significant decrease (P < 0.05) compared to the other groups, while at 14 days partial capsulotomy, the serum BUN reached to the normal value. Serum sodium level showed a significant decrease (P < 0.05) at 2 days after partial capsulotomy, and at 14 days after complete capsulotomy (P < 0.05). Nitric oxide level in IR + partial capsulotomy group showed a significant decrease at 7 and 14 days (P < 0.05). Results of MDA of IR+ partial capsulotomy groups showed a significant decrease (P < 0.05) compared to the IR+ compete capsulotomy groups at 2,7 and 14 days. Conclusion and clinical relevance: The partial capsulotomy ameliorates could improve serum creatinine, BUN and could lower the oxidative stress at 14 days. Partial capsulotomy could also improve the renal tissues at both short and long-term. So this study indicates the importance of the presence of intact renal capsule for ischemic acute kidney injury.

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