Epicuticular Leaf Wax of Euphorbia helioscopia L. (Euphorbiaceae)

1993 ◽  
Vol 48 (1-2) ◽  
pp. 5-9 ◽  
Author(s):  
Muhammad Nazir ◽  
Waqar Ahmad ◽  
Naeem Akhtar Rabi ◽  
Shafiq Ahmad Khan

The epicuticular wax of Euphorbia helioscopia was fractionated into fatty acids, hydrocarbons, wax esters, aldehydes, methyl esters, triterpenol acetates, alcohols, sterols, and polar components. The composition of the fractions was determined by GC, GC-MS, HPLC. Main components within these lipid classes are hentriacontane, wax esters C46 and C48, octacosanal, hexacosanol and octacosanol, hexadecanoic acid, and β-sitosterol. Lupeol and its acetate were also confirmed.

1987 ◽  
Vol 42 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Paul-Gerhard Gülz ◽  
Herbert Hemmers ◽  
Jutta Bodden ◽  
Franz-Josef Marner

Quantity and composition of epicuticular leaf wax of Euphorbia dendroides L. was examined. The wax contained rt-alkanes, wax esters, aldehydes, fatty acids and primary alcohols. In addition to these common epicuticular wax constituents several triterpenoids and benzoic acid esters were found. The triterpenols β-amyrin and lupeol occurred free as well as esterified with long chain fatty acids. The ketones Δ 12-oleanen-3-one and lupen-3-one have also been identified.


1987 ◽  
Vol 42 (1-2) ◽  
pp. 157-158 ◽  
Author(s):  
Thomas Vogt ◽  
Paul-Gerhard Gülz

The epicuticular wax of Cistus albanicus was investigated. Homologous series of alkanes, wax esters, alcohols and long chain fatty acids were identified. The alcohol fraction consisted of primary and most probably of secondary alcohols.


1984 ◽  
Vol 62 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Ellen Sutter

The chemical composition of epicuticular wax from cabbage plants grown in vitro was compared with that from plants grown in a greenhouse. There was a greater percentage of polar compounds (fatty acids, primary alcohols, aldehydes, and esters) and less alkanes and secondary alcohols in wax from cultured plants relative to those from greenhouse plants. Homologues in each class were more evenly distributed and had a greater range of chain lengths in wax from cultured plants. The high proportion of polar components in wax from cultured plants might be a factor in water loss when the plants are transferred to the greenhouse. Possible explanations for the altered wax composition in plants grown in vitro include increased relative humidity and decreased light intensity characteristic of in vitro conditions.


2003 ◽  
Vol 58 (7-8) ◽  
pp. 502-504 ◽  
Author(s):  
Ahmet C. Gören ◽  
Gökhan Bilsel ◽  
Mehmet Altun ◽  
Fatih Satıl

Abstract The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).


1990 ◽  
Vol 45 (7-8) ◽  
pp. 805-812 ◽  
Author(s):  
R. B. N. Prasad ◽  
Paul-Gerhard Giilz

Abstract The leaf cpicuticular waxes of beech trees (Fagus sylvatica L.) were analyzed continuously all over one vegetation period with preparations every week from April 24 to November 15. The folded leaves in buds contained hydrocarbons, wax esters, benzyl acyl esters, alcohols and fatty acids from the beginning, but not aldehydes. Aldehydes were identified only after 10 days of leaf unfolding. The biosynthesis of wax lipids was rapid in the first three to five weeks till May 30. During this time the wax lipids were doubled quantitatively and the chain length spccifity has also changed in all wax lipid classes. From June to October the composition of the wax lipid classes and also the chain length specifity remained constant with the exception of fatty acids. The biosynthesis of wax lipids was found to be very dynamic in the first five weeks and correlated with the leaf development and growth.


1995 ◽  
Vol 118 (1) ◽  
pp. 69-76
Author(s):  
JAVIER VIOQUE ◽  
JULIO E. PASTOR ◽  
EDUARDO VIOQUE
Keyword(s):  

2000 ◽  
Vol 84 (5) ◽  
pp. 781-787 ◽  
Author(s):  
Graham C. Burdge ◽  
Paul Wright ◽  
Amanda E. Jones ◽  
Stephen A. Wootton

Efficient isolation of individual lipid classes is a critical step in the analysis of plasma and lipoprotein fatty acid compositions. Whilst good separations of total lipid extracts are possible by TLC, this method is time consuming and a major rate-limiting step when processing large numbers of specimens. A method for rapid separation of phosphatidylcholine (PC), non-esterified fatty acids (NEFA), cholesterol ester (CE) and triacylglycerol (TAG) from total plasma lipid extracts by solid-phase extraction (SPE) using aminopropyl silica columns has been developed and validated. Following initial separation of polar and neutral lipids, individual classes were isolated by application of solvents with increasing polarity. Recoveries for combined plasma extraction with chloroform–methanol and SPE were (%): PC 74·2 (SD 7·5), NEFA 73·6 (sd 8·3), CE 84·9 (sd 4·9), and TAG 86·8 (sd 4·9), which were significantly greater for TAG and NEFA than by TLC (P<0·001). Both GC–flame ionisation detector and GC-MS analysis of fatty acid methyl esters demonstrated that there was no cross-contamination between lipid classes. Measurements of repeatability of fatty acid composition for TAG, PC, CE and NEFA fractions showed similar CV for each fatty acid. The magnitude of the CV appeared to be related inversely to the fractional fatty acid concentration, and was greatest at concentrations of less than 1 g/100 g total fatty acids. There was no evidence of selective elution of individual fatty acid or CE species. In conclusion, this method represents an efficient, rapid alternative to TLC for isolation of these lipid classes from plasma.


1988 ◽  
Vol 43 (1-2) ◽  
pp. 19-23 ◽  
Author(s):  
Paul-Gerhard Gülz ◽  
Jutta Bodden ◽  
Edith Müller ◽  
Franz-Josef Marner

Quantity and composition of epicuticular wax of Euphorbia aphylla were examined. The wax contained the common lipid components n-alkanes, wax esters, pr. alcohols, fatty acids and in traces aldehydes and acetates all occurring in homologous series. Additionally, several triterpenols such as β-amyrin, α-amyrin, lupeol and simiarenol were found. With the exception of simiarenol these triterpenols occurred free as well as esterified with acetic acid and fatty acids. The ketones, oleanen-3-one, ursen-3-one and lupen-3-one have also been identified


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