scholarly journals CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OIL OF THE Toona sureni (Blume) Merr

2015 ◽  
Vol 3 (1) ◽  
pp. 90
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Germacrene D ◽  
Total Oil

 ABSTRACT The essential oil composition of the Toona sureni (Blume) Merr leaf was analyzed by GC-MS. More than 68 peaks, representing 99.99% of total oil, forty three components were identified, this represents 80.65% of the total oil component. The major components were α-terpinene (9.58%), α-copaene (8.39%), bicyclogermacrene (7.61%), δ-cadinene (6.65%), β-elemene (4.88%), germacrene-D (4.65%), δ-selinene (3.58%), caralene (3.10%), β-caryophyllene (2.88%), α-cubebene (2.82%), δ-gurjunene (2.20%), and (-)-isoledene (2.05%). The antibacterial activity of the essential oil of Toona sureni (Blume) Merr leaf was evaluated using disk diffusion method. The oil was effective on the inactivation of Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Keywords: Toona sureni (Blume) Merr, antibacterial activity, GC-MS, essential oil

scholarly journals Essential Oil Composition and Antibacterial Activity of Hyptis colombiana from the Venezuelan Andes

2015 ◽  
Vol 10 (10) ◽  
pp. 1934578X1501001
Author(s):  
Mayalin Flores ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Maria Eugenia Lucena ◽  
Alfredo Usubillaga
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Sea Level ◽  
Essential Oil Composition ◽  
Minor Constituent ◽  
Oil Composition ◽  
Aerial Parts ◽  
Germacrene D

Hyptis colombiana is an aromatic shrub native to the Colombian and Venezuelan Andes. Aerial parts were collected in Mérida State at about 3100 m above sea level in February 2005, and May and October 2006. The essential oil was found to contain germacrene D and β-caryophyllene as main constituents (about 50%). The February 2005 and October 2006 oils were found to have antibacterial activity against Staphylococcus aureus, but not the May 2006 oil, probably due to the lack of some minor constituent.

scholarly journals Essential Oil Composition and Antibacteral Activity of Vismia Baccifera Fruits Collected from Mérida, Venezuela

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Janne Rojas ◽  
Alexis Buitrago ◽  
Luis Rojas ◽  
Antonio Morales ◽  
María Lucena ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Mass Spectra ◽  
Essential Oil Composition ◽  
Gram Negative Bacteria ◽  
Oil Composition ◽  
Gram Negative

The essential oil from the fruits of Vismia baccifera Triana & Planch. (Gutttiferae), collected in June 2009, was analyzed by GC/MS. A yield of 0.6% oil was obtained by hydrodistillation. Twenty-seven components were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components were trans-cadin-1,4-diene (36.6%), cis-cadin-1,4-diene (18.8%) and β-caryophyllene (11.9%). The essential oil showed a broad spectrum of antibacterial activity against the important human pathogenic Gram-positive and Gram-negative bacteria Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25992), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 23357) with MIC values ranging from 9 to 37 μg/mL.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

scholarly journals Essential Oil Composition and Antibacterial Activity of Monticalia greenmaniana (Asteraceae)

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
José Cárdenas ◽  
Janne Rojas ◽  
Luís Rojas-Fermin ◽  
María Lucena ◽  
Alexis Buitrago
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Mass Spectra ◽  
Essential Oil Composition ◽  
Gram Negative Bacteria ◽  
Oil Composition ◽  
Aerial Parts ◽  
Germacrene D ◽  
Leaf Oil ◽  
Leaf Essential Oil

The essential oils from fresh aerial parts of Monticalia greenmaniana (Hieron) C. Jeffrey (Asteraceae) collected in March, were analyzed by GC/MS. Oil yields (w/v) of 0.1% (flowers), 0.07%, (stems) and 0.1% (leaves) were obtained by hydrodistillation. Thirteen, sixteen and eighteen components, respectively, were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components of the flower and stem oils were 1-nonane (38.8% flowers; 33.5% stems), α-pinene (29.0% flowers; 14.8% stems) and germacrene D (15.6% flowers; 18.6% stems). However, in the leaf oil, germacrene D was observed at 50.7%, followed by β-cedrene at 8.4 %. The leaf essential oil showed a broad spectrum of antibacterial activity against the important human pathogenic Gram-positive and Gram-negative bacteria Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 25955) with MIC values ranging from 75 to 6000 ppm.

scholarly journals Antibacterial Activity and Essential Oil Composition of Satureja spicigera from Iran

2009 ◽  
Vol 64 (1-2) ◽  
pp. 20-24 ◽  
Author(s):  
Fereshteh Eftekhar ◽  
Fereshteh Raei ◽  
Morteza Yousefzadi ◽  
Samad Nejad Ebrahimi ◽  
Javad Hadian
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Resistant Organism ◽  
Disc Diffusion ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Full Flowering

The aerial parts of Satureja spicigera were collected at full flowering stage at Gazvin, Iran. The essential oil was isolated by hydrodistillation and analyzed by a combination of capillary GC and GC-MS. Fourteen compounds were identified, of which carvacrol (53.74%) and thymol (36.03%) were the main constituents, representing 99.12% of the total oil. The in vitro antibacterial activity of the essential oil was determined against six ATCC standard bacterial strains (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) using disc diffusion as well as measurement of minimum inhibitory concentrations. The disc diffusion results and MIC values indicated high inhibitory activity against the test bacteria. The most susceptible organisms were the Gram-positive B. subtilis and S. aureus followed by E. faecalis, usually resistant to most common antibiotics. Among the Gram-negative bacteria, E. coli and K. pneumoniae were highly sensitive to the different oil concentrations in the disc diffusion method. Finally, P. aeruginosa, a highly resistant organism to most antibiotics, showed moderate susceptibility to Satureja spicigera essential oil.

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN SPONS Phyllospongia lamellose SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 252
Author(s):  
Sela S Lempoy ◽  
Widya A Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Isolation And Purification ◽  
Esherichia Coli ◽  
Identification Stage

ABSTRACT Sponges are one source of producing bioactive components from the sea. Bacteria Symbiosis with sponges are thought to have the potential to produce bioactive compounds that have been isolated from sponges. One of the potential of bioactive compounds which have been found and developed from sponges was antibacterial. The aim of this study was to determine the antibacterial activity of bacteria associated with Phyllospongia lamellose sponges against pathogenic bacteria namely Staphylococcus aureus and Esherichia coli and then proceed with identification of biochemistry in isolates which showed the greatest inhibitory activity. Three isolates of sponges symbiont bacteria were obtained through the isolation and purification stage which were then followed by testing of antibacterial activity with paper disk diffusion method. The antibacterial test results showed the diameter of the inhibitory zone against Staphylococcus aureus, were: SL1(8.67 mm), SL2 (9.33 mm) and SL3(9.00 mm) categorized as medium. Whereas the inhibiotion zone shown on Esherichia coli bacteria is also classified as medium, were: SL1(9.67 mm), SL2 (9.00 mm) and SL3 (9.33 mm). The three isolates continued to the identification stage biochemically. Each isolation was assumed as follows: Desulfotomaculum (SL1), Brochothrix (SL2) and Sulfidobacillus (SL3). Keyword         :Phyllospongia lamellose, isolation, biochemically identification ABSTRAK Spons merupakan salah satu sumber penghasil komponen bioaktif yang berasal dari laut. Bakteri yang bersimbiosis dengan spons diduga memiliki potensi dalam menghasilkan senyawa-senyawa bioaktif yang telah diisolasi dari spons. Potensi senyawa bioaktif yang telah ditemukan dan dikembangkan dari spons salah satunya ialah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri yang berasosiasi dengan spons Phyllospongia lamellose terhadap bakteri patogen yakni Staphylococcus aureus dan Escherichia coli dan kemudian dilanjutkan dengan identifikasi secara biokimia pada isolat yang menunjukkan daya hambat terbesar. Diperoleh 3 isolat bakteri simbion spons melalui tahap isolasi dan purifikasi yang kemudian dilanjutkan dengan pengujian aktivitas antibakteri melalui metode difusi kertas cakram. Hasil uji antibakteri menunjukkan diameter zona hambat terhadap bakteri Staphylococcus aureus yaitu: SL1(8.67 mm), SL2(9.33 mm) dan SL3 (9.00 mm) termasuk dalam kategori sedang. Sedangkan zona hambat yang ditunjukkan terhadap bakteri Escherichia coli juga tergolong kategori sedang yaitu: SL1(9.67 mm), SL2(9.00 mm) dan SL3 (9.33 mm). Ketiga isolat dilanjutkan ke tahap identifikasi secara biokimia. Masing-masing isolat diduga sebagai berikut: Desulfotomaculum (SL1), Brochothrix (SL2) dan Sulfidobacillus (SL3). Kata kunci          :Phyllospongia lamellose, isolasi, identifikasi biokimia.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

scholarly journals Cedrus libani ve Pinus nigra subsp. pallasiana’nın Uçucu Yağlarının Kimyasal Bileşimi, Antimikrobiyal Aktivitesi ve Flavonoid İçeriklerinin Belirlenmesi

2020 ◽  
Vol 8 (8) ◽  
pp. 1747-1754
Author(s):  
Ayşe Nur Demirci ◽  
Nazan Çömlekçioğlu ◽  
Ashabil Aygan
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pinus Nigra ◽  
Essential Oil Composition ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disc Diffusion Method ◽  
Cedrus Libani ◽  
Germacrene D

Essential oil composition, antimicrobial activity and flavonoid contents of leaf-fruits of Cedrus libani and Pinus nigra subsp. pallasiana were determined with GC-MS, disc diffusion method and HPLC in three different period. When the essential oil composition of leaf and fruits of P. nigra ssp pallasiana collected in April, July and September, major components were α-pinene, β-pinene, limonene, β-caryophyllene ve germacrene-D. On the other hand, additionally to these contents, myrcene and α–terpineol were also detected in C. libani. According to HPLC analysis, rutin, quercetin, kaempferol, naringin and resveratrol flavonoids were detected in different proportions. While rutin (154.33 µg g-) and resveratrol (20.02 µg g-) has the highest ratio in C. libani, quercetin (9.65 µg g-) and naringin (9.31 µg g-) were detected in P. nigra subsp. pallasiana along with rutin (39.66 µg g-). According to the antimicrobial activity results the essential oils of C. libani obtained in April has produced higher activity than that of July and September. On the contrary, the essential oils from P. nigra subsp. pallasina have produced the best antimicrobial activity on September compared to April and July. As a result, C. libani and P. nigra subsp. pallasiana essential oils have a composition showing antimicrobial activity and their harvesting season should be determined for the best and effective content.

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