scholarly journals Major Constituents and Cytotoxic Effects of Ajuga chamaecistus ssp. tomentella

2012 ◽  
Vol 67 (5-6) ◽  
pp. 275-281 ◽  
Author(s):  
Nargess Sadati ◽  
Kristina Jenett-Siems ◽  
Karsten Siems ◽  
Mohammad Reza Shams Ardekania ◽  
Abbas Hadjiakhoondi ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Cytotoxic Effects ◽  
Aerial Parts ◽  
3T3 Cell Lines ◽  
Nih 3T3 ◽  
Ht 29 ◽  
Tetrazolium Test

The n-butanolic fraction of a methanolic extract (80%) from aerial parts of Ajuga chamaecistus ssp. tomentella was analysed using different chromatographic methods. Column (CC) and high-performance liquid chromatography (HPLC) were used for isolation and purifi cation. 13C, 1H NMR, H-H COSY, HSQC, HMBC, and ESI-MS were employed for identifi cation of the compounds isolated from this fraction. The structures of the compounds were determined to be cis-melilotoside (1), trans-melilotoside (2), lavandulifolioside (3), 20-hydroxyecdysone (4), leonoside B (5), martynoside (6), ajugalactone (7), makisterone A (8), and 24-dehydroprecyasterone (9). This is the fi rst report on the presence of cis- and transmelilotoside in Ajuga species. Cytotoxic evaluation of the n-butanolic fraction, cis- and transmelilotoside against cancer (T47D, HT-29, and Caco-2) and normal (NIH 3T3) cell lines by the mitochondrial tetrazolium test (MTT) showed no cytotoxic effects up to 400 μg/mL. The results of this study suggest that melilotoside, phenylethyl glycosides, and phytoecdysteroids are the main constituents of the n-butanolic fraction of Ajuga chamaecistus ssp. tomentella

scholarly journals Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation

2023 ◽  
Vol 83 ◽  
Author(s):  
M.S. Mahmood ◽  
A. Ashraf ◽  
S. Ali ◽  
A.B. Siddique ◽  
F. Asad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Staph Aureus ◽  
E Coli ◽  
Peel Extract

Abstract Increasing trend in antimicrobial resistance and failure of chemically synthesized antibiotics lead to discover alternative methods for the treatment of bacterial infections. Various medicinal plants are in use traditionally and their active compounds can be further applied for treatment of bacterial diseases. This study was designed to determine the antibacterial activity of Punica granatum (P. granatum L.) (pomegranate) peel extract against Enterobacteriaceae [Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium) and Shigella Dysenteriae (S. Dysenteriae)] and gram-positive bacterium [Staphylococcus aureus (Staph aureus)]. Methanolic extract of P. granatum L. peel was prepared by Soxhlet apparatus method. Total flavonoid and phenolic contents from the extract were determined by High Performance Liquid Chromatography (HPLC). The antibacterial activity of P. granatum L. peel extract was evaluated through agar well diffusion method. HPLC showed the range of phenolics (gallic acid, caffeic acid, benzoic acid, cinnamic acid) and flavonoid compounds. The chemical structures of flavonoid and phenolics found in the methanolic extract of P. granatum L. peel have been reported for the first time. The methanolic peel extract (50 ul) of yellow P. granatum L. showed 26, 10, 10 and 9mm zones of inhibition (ZOI) against S. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. The methanolic extract of red P. granatum L. (100 ul) showed 27, 8, 12 and 15 mm ZOI against Staph. aureus, S. Typhimurium, S. Dysenteriae and E. coli, respectively. Highest ZOI was observed against Staph. aureus. Many of the bacteria studied in the present work may cause serious gastrointestinal infections, which can lead to hemorrhagic diarrhea in children. These infections can be life-threatening to young children and the elderly. There is an incentive to find alternative control measures, such as plant and herbal extracts, especially in lesser-developed countries where traditional antibiotics may not be readily available.

scholarly journals Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Raman Preet ◽  
Raghbir Chand Gupta
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Mobile Phase ◽  
High Performance ◽  
Isocratic Elution ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Whole Plant

During the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optimized process was used for the quantification of ten phenolic compounds. The chromatographic separation was accomplished on an Atlantis T3 column at 25°C with isocratic elution. A mixture of acetonitrile and water was used as the mobile phase at a flow rate of 0.8 mL/min. The linear regression examination data for the calibration plots displayed a good linear relationship with r2 > 0.999 in the concentration range of 2–20 µL. In the methanolic extracts of the whole plant of L. pyrotechnica, the content of caffeic acid (3.3%) was reported to be the highest concentration.

scholarly journals DISSIPAÇÃO DO HERBICIDA (R,S) HALOXIFOPE EM LATOSSOLO TROPICAL

2004 ◽  
Vol 14 ◽  
Author(s):  
MARCUS BARIFOUSE MATALLO ◽  
LUIZ CARLOS LUCHINI ◽  
FLAVIO MARTINS GARCIA BLANCO ◽  
TEREZINHA BONANHO PERES ◽  
DANIEL GUSTAVO RICHENA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Half Life ◽  
High Performance ◽  
Methanolic Extract ◽  
Tropical Soil ◽  
Soil Samples ◽  
Ultra Sound ◽  
Previously Treated ◽  
Para Determinar

Com o objetivo de estudar o comportamento do herbicida (R,S)-haloxifope em solo tropical realizouse este trabalho para determinar sua dissipação num latossolo roxo eutrófico. Para isso, amostras de solo (em triplicata) foram previamente tratadas com haloxifope metil racêmico e incubadas a 25oC por 0, 6, 10 15, 30 e 60 dias. Após cada período de incubação as amostras foram submetidas a extração em ultra-som, purificadas com diclorometano, evaporadas em rotavapor e ressuspendidas em metanol. O extrato metanólico foi analisado por Cromatografia a Líquido de Alta Eficiência (CLAE). Os dados cinéticos mostraram meia-vida inicial (t0½) de 7,38 dias, o que pode estar relacionado com a atividade microbiana de caráter enantiosseletivo no solo. (R,S)-HALOXYFOP DISSIPATION IN A TROPICAL LATOSOIL Abstract The aim of the work was to study the behavior of the herbicide (R,S)-haloxyfop in a tropical soil. This work was realized to determine its dissipitaion in an eutrophic purple latosoil. Three replicates of soil samples were previously treated with methyl racemic haloxyfop and incubated at 25ºC for 0, 6, 10, 15, 30 and 60 days. After each incubation period the samples were submited to ultra sound extraction, purified with dichoromethane, evaporated and ressuspended in methanol. The methanolic extract was analysed by High Performance Liquid Chromatography (HPLC). The kinetics data revealed initial half life (t01/2) of 7.38 days, which can probably be related to the microbial activity with enantioselective character in soil.

scholarly journals DETERMINATION OF LUTEOLIN FROM EXTRACTIONS OF Helicteres hirsuta BY HPLC

2019 ◽  
Vol 128 (1B) ◽  
pp. 43
Author(s):  
Lê Trung Hiếu ◽  
Lê Lâm Sơn ◽  
Nguyễn Minh Nhung ◽  
Hồ Xuân Anh Vũ ◽  
Trần Thị Văn Thi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Array Detector ◽  
Plant Parts ◽  
Aerial Parts ◽  
Photodiode Array Detector ◽  
Photodiode Array

<p>High performance liquid chromatography coupled with photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in extractive solutions from <em>Helicteres hirsuta</em>. Luteolin was analyzed on a RP- C18 column, using a mobile phase including: acetonitrile - 0.1% acid phosphoric (v/v), under the following conditions detecting wavelength: 347 nm; flow rate: 0.5 mL/min, and the volume of injecting sample: 10 μL. The HPLC system was carried out at ambient temperature. The method showed linearity for luteolin in the range to 0.02 from 1 mg/mL, and the recovery of luteolin was 94.07 ± 0.64. Contents of  luteolin in methanol extracts from the plant parts of <em>H. hirsuta</em> (including: branch, leaf, fruit and aerial parts) were determined with value of 49.06 ±0.46, 142.89 ±0.53, 56.61±0.62 and 91.15±0.42 µg/g, respectively.</p>

Ultra-high-performance liquid chromatography – high-resolution mass spectrometry profiling and hepatoprotective activity of purified saponin and flavonoid fractions from the aerial parts of wild spinach (Chenopodium bonus-henricus L.)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Zlatina Kokanova-Nedialkova ◽  
Paraskev Nedialkov ◽  
Magdalena Kondeva-Burdina
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Hepatoprotective Activity ◽  
Aerial Parts ◽  
High Resolution Mass ◽  
Resolution Mass

AbstractAn ultra-high-performance liquid chromatography – high-resolution mass spectrometry profiling method was used for a comprehensive study of flavonoid and saponin-rich fractions from the aerial parts of wild spinach (Chenopodium bonus-henricus L.). Thirty-six compounds, respectively, 22 saponins of eight sapogenins (phytolaccagenin, bayogenin, medicagenic acid, 2β-hydroxygypsogenin, 2β-hydroxyoleanoic acid, 2-hydroxy-30-nor-gypsogenin, 2-hydroxyakebonic acid, and akebonic acid) together with 12 flavonoid glycosides of 6-methoxykaempferol, isorhamnetin, patuletin, spinacetin as well as two ecdysteroids (20-hydroxyecdysone and polypodine B) were detected. The occurrence of sapogenins 2-hydroxy-30-nor-gypsogenin, 2-hydroxyakebonic acid, and akebonic acid in the Chenopodium genus is reported here for the first time. The flavonoid and saponin-rich fractions showed in vitro hepatoprotective and antioxidant activity comparable to those of flavonoid complex silymarin (60 μg/mL) in a model of metabolic bioactivation, induced by CCl4. All tested fractions, compared to silymarin, significantly reduced the cellular damage caused by CCl4 in rat hepatocytes, preserved cell viability and GSH level, decreased LDH leakage, and reduced lipid damage. The results showed that saponin-rich fractions F3A and F3B possessed better hepatoprotective activity than flavonoid-rich fractions (F2A and F2B). The most active was fraction F3B and this is probably due to the synergism between the saponins and some acylated flavonol glycosides found there.

scholarly journals High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract

2021 ◽  
pp. 32-40
Author(s):  
Abdullah Alamami ◽  
Fatma Elshibani ◽  
Salmin Alshalmani ◽  
Mohamed Ali Sharkasi ◽  
Naema Elremali ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Cistus Salviifolius

Aims: This research is focused on the in vitro evaluation of Cistus salviifolius L. antimicrobial properties and the determination of the contents of phenols and flavonoids. Study Design: This research is analytical study aimed to illustrate the antimicrobial properties and to analyze the methanolic extract contents of aerial parts of Cistus salviifolius L. by high-performance liquid chromatography techniques. Duration: The study was performed within six months in the Faculty of Pharmacy, Benghazy University. Methodology: Antimicrobial properties was tested against twelve organisms using Kirby-Bauer disk diffusion sensitivity test and the determination of the contents of phenols and flavonoids was evaluated by running high-performance liquid chromatography techniques. Results: The findings indicated that catechin is the most abundant flavonoid in C. salviifolius, while gallic acid was the major phenol in the methanolic extract of the plant. The results also revealed that the methanol extracts had a significant antimicrobial potential particularly against Bacillus subtilis and Escherichia coli with MIC (0.98 and 0.49) µg/ml respectively, furthermore the extract was effective against Aspergillus fumigatus with MIC 0.98 µg/ml.       Conclusion: C. salviifolius was highly rich with flavonoids and phenols and has a significant antimicrobial effect.

Sign in / Sign up

Export Citation Format

Share Document