Regional and State Needs in Sampling and Analytical Methodology

Background: Metabolomics is one of the main areas to understand cellular process at molecular level by analyzing metabolites. In recent years metabolomics has been emerged as key tool to understand molecular basis of disease, find diagnostic and prognostic biomarkers, and develop new treatment opportunities and drug molecules. Objective: In this study, an untargeted metabolite and lipid analysis were performed to identify potential biomarkers on premature ovarian insufficiency plasma samples. 43 POI subject plasma samples were compared with 32 healthy subject plasma samples. Methods: Plasma samples were pooled and extracted using chloroform:methanol:water (3:3:1 v/v/v) mixture. Agilent 6530 LC/MS Q-TOF instrument equipped with ESI source was used for analysis. A C18 column (Agilent Zorbax 1.8 μM, 50 x 2.1 mm) was used for separation of metabolites and lipids. XCMS, an “R software” based freeware program, was used for peak picking, grouping and comparing the findings. Isotopologue Parameter Optimization (IPO) software was used in order to optimize XCMS parameters. The analytical methodology and data mining process were validated according to the literature. Results: 83 metabolite peaks and 213 lipid peaks were found to be in semi-quantitatively and statistically different (fold change >1.5, p <0.05) between the POI plasma samples and control subjects. Conclusion: According to the results, two groups were successfully separated through principal component analysis. Among the peaks, phenyl alanine, decanoyl-L-carnitine, 1-palmitoyllysophosphatidylcholine and PC(O-16:0/2:0) were identified through auto MS/MS and matched with human metabolome database and proposed as plasma biomarker for POI and monitoring the patients in treatment period.

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Calcium/Copper alginate framework doped with CuO nano-particles as a novel adsorbent for micro-extraction of benzodiazepines from human serum

Current Pharmaceutical Analysis ◽

10.2174/1573412916666200210150914 ◽

2020 ◽

Vol 16 ◽

Author(s):

Nadereh Rahbar ◽

Fatemeh Ahmadi ◽

Zahra Ramezani ◽

Masoumeh Nourani

Keyword(s):

Human Serum ◽

High Performance ◽

Limit Of Detection ◽

Solid Phase ◽

Nano Particles ◽

Limit Of Quantification ◽

Analytical Methodology ◽

Fiber Fabrication ◽

Relative Standard ◽

Green Procedure

Background: Sample preparation is one of the most challenging phases in pharmaceutical analysis, especially in biological matrices, affecting the whole analytical methodology. Objective: In this study, a new Ca(II)/Cu(II)/alginate/CuO nanoparticles hydrogel fiber (CCACHF) was synthesized through a simple, green procedure and applied for fiber micro solid phase extraction (FMSPE) of diazepam (DIZ) and oxazepam (OXZ) as model drugs prior to high-performance liquid chromatography-UV detection (HPLC-UV). Methods: Composition and morphology of the prepared fiber were characterized and the effect of main parameters on the fiber fabrication and extraction efficiency have been studied and optimized. Results: In optimal conditions, calibration curves were linear ranging between 0.1–500 µg L−1 with regression coefficients of 0.9938 and 0.9968. Limit of detection (LOD) (S/N=3) and limit of quantification (LOQ) (S/N=10) of the technique for DIZ and OXZ were 0.03 to 0.1 µg L−1. Within-day and between-day relative standard deviations (RSDs) for DIZ and OXZ were 6.0–12.5% and 3.3–9.4%, respectively. Conclusion: The fabricated adsorbent has been substantially employed to extraction of selected benzo-diazepines (BZDs) from human serum real specimens and the obtained recoveries were also satisfactory (82.1-109.7%).

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El Papel de Humbodt en el Origen y Desarrollo de la Geografia Moderna

GEOgraphia ◽

10.22409/geographia2002.48.a13430 ◽

2009 ◽

Vol 4 (8) ◽

pp. 7

Author(s):

Héctor F. Rucinque e Wellington Jiménez

Keyword(s):

Key Words ◽

Founding Father ◽

Analytical Methodology ◽

Alexander Von Humboldt ◽

Scientific Exploration ◽

History Of Geography ◽

Tropical America ◽

History Of ◽

Scholarly Production ◽

Modern Geography

RESUMO Por lo general, los historiadores de la ciencia reconocen la importaocia de Alexander von Humboldt en el desarrollo de la geografía moderna, si bien tal contribución especializada no es claramente desglosada de su multifacética producción científica. Con ocasión del bicentenario de su viaje a la América tropical, el papel de Humboldt en la formulación de las bases de una metodología analítica para la investigación geográfica, y su monumental trabajo sustantivo, lo mismo que su penetrante permanencia e inspiración en la tradición geográfica, deben acreditarse como justificación amplia y suficiente para su título de padre fundador de la geografía científica. Epígrafes: Humboldt, historia de la geografía, geografía moderna, metodología geográfica, exploración científica.ABSTRACT Alexander von Humboldt’s contributions to the development of modern geography are generally ackoowledged by historians of science, though not always stated precisely out of his many-sided scholarly production. On the occasion of the Bicentennial of his voyage to tropical America, Humboldt’s role in setting forth the foundation of an analytical methodology for geography as well as for his monumental substantive work, along with his pervasive and inspiring perrnanence in the geographical tradition, must be recognized as ample justification tu his title as founding father of scientific geography. Key words: Humboldt, history of geography, modern geography, geagraphical methodology, scientific exploration.

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Educational Pursuits

10.1093/oso/9780198800149.003.0002 ◽

2017 ◽

Author(s):

Howell A. Lloyd

Keyword(s):

Principal Components ◽

Ongoing Debate ◽

Analytical Methodology ◽

Jean Bodin ◽

University Of Paris ◽

The University ◽

Educational Pursuits

The chapter opens with a brief description of Paris at around the time of Bodin’s arrival. It notes the location of the Carmelite house, near the colleges of the University of Paris, and specifies intellectual influences at work there, both humanist and scholastic. They included ongoing debate over key philosophical, theological, and jurisprudential issues to consideration of which Bodin would have been exposed. They also included debates over the proper use of language, over modes of literary presentation, and over analytical methodology. The contribution of Pierre de La Ramée (Ramus) to these debates is examined, and the principal components of his celebrated ‘method’ are identified. Attention is drawn to the continued importance of Aristotle in these areas of thought and instruction. Finally, the question is examined whether the ‘Jehan Baudin’ arrested and imprisoned in 1548 as a suspect heretic was in fact the Carmelite novice, Jean Bodin of Angers.

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Yaw Galloping of a TLWP Platform Under High Speed Currents by Analytical Methods and its Comparison With Experimental Results

Volume 1: Offshore Technology ◽

10.1115/omae2017-61909 ◽

2017 ◽

Author(s):

Francisco Lamas ◽

Miguel A. M. Ramirez ◽

Antonio Carlos Fernandes

Keyword(s):

High Speed ◽

Production Systems ◽

Experimental Tests ◽

Experimental Results ◽

Analytical Methodology ◽

New Approach ◽

Laboratory Facilities ◽

Polynomial Curve ◽

Computational Fluid Dynamics Cfd ◽

Cfd Analyses

Flow Induced Motions are always an important subject during both design and operational phases of an offshore platform life. These motions could significantly affect the performance of the platform, including its mooring and oil production systems. These kind of analyses are performed using basically two different approaches: experimental tests with reduced models and, more recently, with Computational Fluid Dynamics (CFD) dynamic analysis. The main objective of this work is to present a new approach, based on an analytical methodology using static CFD analyses to estimate the response on yaw motions of a Tension Leg Wellhead Platform on one of the several types of motions that can be classified as flow-induced motions, known as galloping. The first step is to review the equations that govern the yaw motions of an ocean platform when subjected to currents from different angles of attack. The yaw moment coefficients will be obtained using CFD steady-state analysis, on which the yaw moments will be calculated for several angles of attack, placed around the central angle where the analysis is being carried out. Having the force coefficients plotted against the angle values, we can adjust a polynomial curve around each analysis point in order to evaluate the amplitude of the yaw motion using a limit cycle approach. Other properties of the system which are flow-dependent, such as damping and added mass, will also be estimated using CFD. The last part of this work consists in comparing the analytical results with experimental results obtained at the LOC/COPPE-UFRJ laboratory facilities.

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Comparison of 2 glucose analytical methodologies in immature Kemp’s ridley sea turtles: dry chemistry of plasma versus point-of-care glucometer analysis of whole blood

Journal of Veterinary Diagnostic Investigation ◽

10.1177/10406387211001830 ◽

2021 ◽

pp. 104063872110018

Author(s):

Justin R. Perrault ◽

Michael D. Arendt ◽

Jeffrey A. Schwenter ◽

Julia L. Byrd ◽

Kathryn A. Tuxbury ◽

...

Keyword(s):

Whole Blood ◽

Point Of Care ◽

Sea Turtles ◽

Sample Volume ◽

Specific Reference ◽

Volume Data ◽

Analytical Methodology ◽

Glucose Determination ◽

Kemp's Ridley Sea Turtles ◽

Kemp's Ridley

Blood glucose measurements provide important diagnostic information regarding stress, disease, and nutritional status. Glucose analytical methodologies include dry chemistry analysis (DCA) of plasma and point-of-care (POC) glucometer analysis of whole blood; however, these 2 methods differ in cost, required sample volume, and processing time. Because POC glucometers use built-in equations based on features of mammalian blood to convert whole blood measurements to plasma equivalent units, obtained glucose data must be compared and validated using gold-standard chemistry analytical methodology in reptiles. For in-water, trawl-captured, immature Kemp’s ridley sea turtles ( Lepidochelys kempii) from Georgia, USA, we observed significant, positive agreement between the 2 glucose determination methods; however, the glucometer overestimated glucose concentrations by 1.4 mmol/L on average in comparison to DCA and produced a wider range of results. The discordance of these results suggests that POC glucometer glucose data should be interpreted in the context of methodology- and brand-specific reference intervals along with concurrent packed cell volume data.

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A simple analytical methodology for platinum nanoparticles control in complex clinical matrices via SP-ICP-MS

Talanta ◽

10.1016/j.talanta.2021.122370 ◽

2021 ◽

pp. 122370

Author(s):

Sergio Fernández-Trujillo ◽

María Jiménez-Moreno ◽

Ángel Ríos ◽

Rosa del Carmen Rodríguez Martín-Doimeadios

Keyword(s):

Platinum Nanoparticles ◽

Analytical Methodology ◽

Icp Ms

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