Effects of seed storage conditions on seed water uptake, germination and vigour in Pinus dabeshanensis, an endangered pine endemic to China

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

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Annual dormancy cycles in Lesquerella fendleri (Brassicaceae) seeds stored under both field and laboratory conditions

Canadian Journal of Botany ◽

10.1139/b99-142 ◽

2000 ◽

Vol 77 (11) ◽

pp. 1648-1654

Author(s):

Laura A Hyatt ◽

Ann S Evans ◽

Carol C Baskin

Keyword(s):

Seed Storage ◽

Storage Conditions ◽

Perennial Species ◽

Lesquerella Fendleri ◽

Laboratory Conditions ◽

Fluctuating Environments ◽

Growth Chambers ◽

Field And Laboratory Conditions ◽

Dormancy Cycles ◽

Over Time

Patterns of germination over time were investigated in a short-lived desert perennial species, Lesquerella fendleri (Gray) S. Wats. Field-collected seeds were either buried in the field in cloth bags or stored in a glass jar under laboratory conditions. Regular germination tests were conducted under a range of alternating temperatures (buried seeds) or under differing water regimes (laboratory-stored seeds). Testing revealed Lesquerella fendleri to have an annual dormancy-nondormancy cycle, which was manifest regardless of seed storage conditions, suggesting that cycles may be partially endogenously regulated. Increasing seed germinability in field-stored seeds (maximum of 30% in year 1 and 95% in year 2) leads to the formation of a seed bank for at least 1 year and enhances the possibility of spreading germination events through time. This allows parent plants to maximize fitness in randomly fluctuating environments. The existence of two seedling flushes in the field and single germinability peaks in growth chambers suggests that dormancy cycling may support the formation of a metapopulation with subpopulations which are, although physically intermingled, genetically distinct.

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High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

Mediterranean Botany ◽

10.5209/mbot.69341 ◽

2021 ◽

Vol 42 ◽

pp. e69341

Author(s):

Miguel Ángel González Pérez ◽

Nereida Cabrera-García ◽

Isabel Cayon-Fernández

Keyword(s):

Seed Bank ◽

Germination Rate ◽

Genetic Material ◽

Natural Populations ◽

Seed Viability ◽

Seed Storage ◽

Botanical Garden ◽

Storage Conditions ◽

Seed Banks ◽

Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

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Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration

Plant Methods ◽

10.1186/s13007-019-0541-6 ◽

2019 ◽

Vol 15 (1) ◽

Cited By ~ 1

Author(s):

Shidong Yue ◽

Yu Zhang ◽

Yi Zhou ◽

Shaochun Xu ◽

Shuai Xu ◽

...

Keyword(s):

Seed Storage ◽

Storage Conditions ◽

Seed Vigor ◽

Ex Situ ◽

Zostera Japonica ◽

Storage Method ◽

Seagrass Meadows ◽

Long Term Storage ◽

Term Storage

Abstract Background Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as – 5 °C. However, our results indicated that reduced storage temperature to 0 °C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 °C under a salinity of 40–60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 °C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 ± 16.8%. Conclusion Our experiments demonstrated that seeds stored at 0 °C under a salinity of 40–60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

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Analysis of seed-bank data confirms suitability of international seed-storage standards for the Australian flora

Australian Journal of Botany ◽

10.1071/bt06038 ◽

2007 ◽

Vol 55 (1) ◽

pp. 18 ◽

Cited By ~ 30

Author(s):

Andrew D. Crawford ◽

Kathryn J. Steadman ◽

Julie A. Plummer ◽

Anne Cochrane ◽

Robin J. Probert

Keyword(s):

Western Australia ◽

Seed Bank ◽

Seed Storage ◽

Storage Conditions ◽

International Standards ◽

Australian Flora ◽

Western Australian

The suitability of applying international standards for long-term seed storage to the Australian flora was examined by reviewing seed-storage data from the Western Australian Threatened Flora Seed Centre. The 375 collections examined represented 176 taxa from 44 genera and 16 families. A high proportion of collections, representative of some of the most common genera in Australia, maintained viability in the short (<5 years) and medium (5–12 years) term. Declines in germination were evident for a small number of collections, representing 10 taxa, stored for 5–12 years. However, many of the declines were collection-specific and other collections of the same taxon did not decline. Five taxa showing germination declines were represented by a single collection so it was not possible to determine whether the germination decline was directly related to the taxa, storage conditions or to other factors. Only the closely related Stylidium coroniforme and S. amabile exhibited germination declines in all collections, but cut tests of seeds remaining from germination testing indicated that viability of the collections had not declined, just the proportion to germinate; for these species a change in germination conditions is a more likely explanation for the reduction in germination. The results illustrate the successful application of these seed-storage standards to threatened flora in Western Australia and highlight their suitability for the Australian flora.

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Evaluation of seed storage conditions on the growth of rough lemon (citrus jambhiri) rootstock seedlings

Nigerian Journal of Horticultural Science ◽

10.4314/njhs.v9i1.3380 ◽

2005 ◽

Vol 9 (1) ◽

Author(s):

RKA Egharevba ◽

M Aiyeduyoni

Keyword(s):

Seed Storage ◽

Storage Conditions ◽

Citrus Jambhiri ◽

Rough Lemon

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Effects of Shell and Low Moisture Content on Peanut Seed Longevity

Peanut Science ◽

10.3146/pnut.29.2.0008 ◽

2002 ◽

Vol 29 (2) ◽

pp. 122-125 ◽

Cited By ~ 4

Author(s):

N. Kameswara Rao ◽

D. V. S. S. R. Sastry ◽

P. J. Bramel

Keyword(s):

Moisture Content ◽

Low Cost ◽

Seed Storage ◽

Cost Effective ◽

Storage Conditions ◽

Medium Term ◽

Short Term ◽

Effective Strategies ◽

Arachis Hypogaea L ◽

High Germination

Abstract Peanut (Arachis hypogaea L.) germplasm accessions in ICRISAT genebank are conserved as pods under medium-term conditions (4 C and 30% RH). Pod storage requires far greater space than seed storage and is more likely to be expensive, especially in a controlled environment. With the objective to evolve cost effective strategies for conservation, the survival of in-shell and shelled seeds of two peanut cultivars, ICGS 76 (virginia bunch) and JL24 (spanish), was studied under three different storage conditions— ambient (20-40 C and 30-80% RH), short term (23-25 C and 40-50% RH), and medium term (4 C and 30% RH). In-shell seeds had marginally greater longevity than shelled seed in all storage conditions. The differences in time for regeneration of in-shell and shelled seeds stored under medium term conditions were estimated to be less than 4 mo for both the cultivars. Because of the much reduced volume required for storage and the insignificant differences in regeneration interval, conservation of shelled seeds would be highly cost-effective under the controlled environmental conditions, as compared to in-shell seeds. Since storage at very low moisture contents was suggested as a simple and low cost option for conservation of seed lots required for short-term use, the longevity of peanut seeds (cv. ICGS 76) hermetically sealed with 3.6% moisture content was studied in comparison with seeds held at 5.8% moisture. The studies showed that peanut seeds hermetically stored at room temperature (23-25 C) with low moisture content (below 4%) could retain high germination (> 85%) for up to 8 yr.

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Standardization of seed storage conditions in chilgoza pine (Pinus gerardiana Wall.): an endangered pine of Hind Kush Himalaya

Trees ◽

10.1007/s00468-013-0868-y ◽

2013 ◽

Vol 27 (5) ◽

pp. 1497-1501 ◽

Cited By ~ 2

Author(s):

A. R. Malik ◽

G. S. Shamet ◽

Jitendra S. Butola ◽

G. M. Bhat ◽

A. A. Mir ◽

...

Keyword(s):

Seed Storage ◽

Storage Conditions ◽

Pinus Gerardiana

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Prediction and Determination of Water Uptake in Packaged Yerba Mate

Food Science and Technology International ◽

10.1177/1082013204041877 ◽

2004 ◽

Vol 10 (1) ◽

pp. 35-40 ◽

Cited By ~ 4

Author(s):

L. A. Ramallo ◽

O. A. Albani

Keyword(s):

Water Vapour ◽

Water Uptake ◽

Transmission Rate ◽

Storage Conditions ◽

Yerba Mate ◽

Water Vapour Transmission Rate ◽

Experimental Values ◽

Temperature Water ◽

Good Agreement

Permeability of different packaging materials and the water uptake were studied in stored yerba mate at different conditions of humidity and temperature. Water vapour transmission rate (WVTR) was evaluated using the quasi-isostatic method. During storage at high relative humidity conditions, WVTR values were of 500 gm2/day for paper and ranged from 16 to 22 gm2/day for laminated films. Values of package permeability and data of food isotherms were used to calculate the variation of internal water vapour activity and to predict the yerba mate water uptake. The predicted and experimental values were in good agreement. For similar high humidity storage conditions, yerba mate packaged with paper reached critical humidity content (9.5% d.b.) at the second day of storage whereas for the laminate films the humidity content stood below the critical value until a month of exposure.

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Seed and Embryo Germination in Ardisia crenata

Journal of Botany ◽

10.1155/2012/679765 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Takahiro Tezuka ◽

Hisa Yokoyama ◽

Hideyuki Tanaka ◽

Shuji Shiozaki ◽

Masayuki Oda

Keyword(s):

Seed Germination ◽

Seed Coat ◽

Critical Factor ◽

Seed Storage ◽

Storage Conditions ◽

Embryo Germination ◽

Long Time ◽

Dry Conditions ◽

Temperature Water ◽

Good Germination

Ardisia crenata is an evergreen shrub with attractive bright red berries. Although this species is usually propagated by seed, the seeds take a long time to germinate with conventional sowing methods. We investigated the germination capacity of seeds and embryos collected in different months and the effects of seed storage conditions, germination temperature, water permeability of the seed coat, and the endosperm on seed germination. Seeds and embryos collected in late September or later showed good germination rates. Seeds germinated more rapidly after longer periods of storage at low temperature (approximately 5°C), and those stored in dry conditions showed lower emergence frequency than those stored in wet conditions. Seeds germinated at 15–30°C, but not at 5–10°C. Removal of the seed coat enhanced water uptake and seed germination. Seeds with various proportions of the removed seed coat were sown on a medium supplemented with sucrose. The germination frequency increased as the size of the remaining endosperm decreased. However, the opposite results were obtained when seeds were sown on a medium without sucrose. We concluded that the optimal temperature of 25°C is the most critical factor for seed germination in A. crenata.

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