Differential expression of mRNA of the vitamin D receptor, 1[alpha]-hydroxylase 25-hydroxy vitamin D and estrogen receptors in human explants and cell cultures of thyroid cancer and normal tissues

2016 ◽  
Author(s):  
Dalia Somjen ◽  
Orli Sharon ◽  
Esther Knoll ◽  
Abraham Aizic ◽  
David Fliss ◽  
...  
Keyword(s):  
Vitamin D ◽  
Thyroid Cancer ◽  
Estrogen Receptors ◽  
Differential Expression ◽  
Cell Cultures ◽  
Vitamin D Receptor ◽  
Normal Tissues ◽  
25 Hydroxy Vitamin D ◽  
Human Explants

scholarly journals 25-Hydroxy Vitamin D, Vitamin D Receptor and Toll-like Receptor 2 Polymorphisms in Spinal Tuberculosis

Medicine ◽  
2016 ◽  
Vol 95 (17) ◽  
pp. e3418 ◽  
Author(s):  
Ajay Panwar ◽  
Ravindra Kumar Garg ◽  
Hardeep Singh Malhotra ◽  
Amita Jain ◽  
Arvind Kumar Singh ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Spinal Tuberculosis ◽  
Toll Like Receptor ◽  
25 Hydroxy Vitamin D ◽  
Toll Like Receptor 2

scholarly journals VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 954 ◽  
Author(s):  
Leonardo P. Stuchi ◽  
Márcia Maria U. Castanhole-Nunes ◽  
Nathália Maniezzo-Stuchi ◽  
Patrícia M. Biselli-Chicote ◽  
Tiago Henrique ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Differential Expression ◽  
Cancer Progression ◽  
Tissue Samples ◽  
Protein Levels ◽  
Normal Tissues ◽  
Thyroid Papillary ◽  
Related Transcription Factor ◽  
Thyroid Papillary Cancer ◽  
Endothelial Growth Factor

Deregulation of VEGFA (Vascular Endothelial Growth Factor A) and NFE2L2 (Nuclear Factor (Erythroid-derived 2)-Like 2), involved in angiogenesis and oxidative stress, can lead to thyroid cancer progression. MiR-17-5p and miR-612 are possible regulators of these genes and may promote thyroid disorders. In order to evaluate the involvement of VEGFA, NFE2L2, hsa-miR-17-5p, and hsa-miR-612 in thyroid pathology, we examined tissue samples from colloid goiter, papillary thyroid cancer (PTC), and a normal thyroid. We found higher levels of VEGFA and NFE2L2 transcripts and the VEGFA protein in goiter and PTC samples than in normal tissue. In the goiter, miR-612 and miR-17-5p levels were lower than those in PTC. Tumors, despite showing lower VEGFA mRNA expression, presented higher VEGFA protein levels compared to goiter tissue. In addition, NRF2 (Nuclear Related Transcription Factor 2) protein levels in tumors were higher than those in goiter and normal tissues. Inhibition of miR-17-5p resulted in reduced NFE2L2 expression. Overall, both transcript and protein levels of NFE2L2 and VEGFA were elevated in PTC and colloid goiter. Hsa-miR-612 showed differential expression in PTC and colloid goiter, while hsa-miR-17-5p showed differential expression only in colloid goiter, suggesting that hsa-miR-17-5p may be a positive regulator of NFE2L2 expression in PTC.

scholarly journals BsmI polymorphism in the vitamin D receptor gene is associated with 25-hydroxy vitamin D levels in individuals with cognitive decline

2018 ◽  
Vol 76 (11) ◽  
pp. 760-766 ◽  
Author(s):  
Ana Carolina R. de Oliveira ◽  
Carolina A. Magalhães ◽  
Cristina M. G. Loures ◽  
Vanessa G. Fraga ◽  
Leonardo C. de Souza ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cognitive Decline ◽  
Vitamin D Receptor ◽  
Gene Polymorphisms ◽  
Vdr Gene ◽  
Bsmi Polymorphism ◽  
Vitamin D Levels ◽  
Vdr Gene Polymorphisms ◽  
Significant Difference ◽  
25 Hydroxy Vitamin D

ABSTRACT Elderly people are at a high risk of developing vitamin D (VitD) deficiency due to both decreased intake and cutaneous synthesis. Most of the biological actions of VitD are mediated by the vitamin D receptor (VDR), which is present in neurons and glial cells of the hippocampus, and in the cortex and subcortical nuclei, essential areas for cognition. It is known that VDR gene polymorphisms may decrease the VDR affinity for VitD. Objective: The present study aimed to investigate the influence of VitD levels on cognitive decline in patients with dementia due to Alzheimer's disease (AD, n = 32) and mild cognitive impairment (MCI, n = 15) compared to cognitively healthy elderly (n = 24). We also evaluated the association of VDR gene polymorphisms with cognitive disturbance. Methods: Four polymorphisms on the VDR gene were studied, namely, BsmI, ApaI, FokI and TaqI, by polymerase chain reaction-restriction fragment length polymorphism. Serum levels of 25-hydroxy vitamin D (25(OH)D) were determined by high performance liquid chromatography. Results: No significant difference in 25(OH)D levels or genotypic/allelic frequencies was observed between the groups. Deficiency of 25(OH)D was more frequently observed in women. The AA/AG genotypes of the BsmI polymorphism was associated with sufficient 25(OH)D levels, while the GG genotype of this same polymorphism was associated to insufficient levels in the cognitively-impaired group (individuals with AD or MCI). Conclusions: The data obtained do not confirm the relationship between reductions of VitD levels, polymorphisms in the VDR gene, and altered cognitive function in this sample. However, the data indicate that BsmI polymorphism in the VDR gene is associated with the VitD levels in individuals with cognitive decline.

scholarly journals Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells

2020 ◽  
Vol 10 (8) ◽  
pp. 545 ◽  
Author(s):  
Tsung-Ming Hu ◽  
Hsiang-Sheng Chung ◽  
Lieh-Yung Ping ◽  
Shih-Hsin Hsu ◽  
Hsin-Yao Tsai ◽  
...  
Keyword(s):  
Vitamin D ◽  
Valproic Acid ◽  
Differential Expression ◽  
Vitamin D Receptor ◽  
Ribosome Biogenesis ◽  
Molecular Mechanisms ◽  
Neuroblastoma Cells ◽  
Absolute Quantitation ◽  
Signaling Systems ◽  
Isobaric Tags

Valproic acid (VPA) is a multifunctional medication used for the treatment of epilepsy, mania associated with bipolar disorder, and migraine. The pharmacological effects of VPA involve a variety of neurotransmitter and cell signaling systems, but the molecular mechanisms underlying its clinical efficacy is to date largely unknown. In this study, we used the isobaric tags for relative and absolute quantitation shotgun proteomic analysis to screen differentially expressed proteins in VPA-treated SH-SY5Y cells. We identified changes in the expression levels of multiple proteins involved in Alzheimer’s disease, Parkinson’s disease, chromatin remodeling, controlling gene expression via the vitamin D receptor, ribosome biogenesis, ubiquitin-mediated proteolysis, and the mitochondrial oxidative phosphorylation and electron transport chain. Our data indicate that VPA may modulate the differential expression of proteins involved in mitochondrial function and vitamin D receptor-mediated chromatin transcriptional regulation and proteins implicated in the pathogenesis of neurodegenerative diseases.

Plasma 25-hydroxy vitamin D concentrations, vitamin D receptor genotype and breast cancer risk in a UK Caucasian population

2005 ◽  
Vol 41 (8) ◽  
pp. 1164-1169 ◽  
Author(s):  
Lorraine C. Lowe ◽  
Michelle Guy ◽  
Janine L. Mansi ◽  
Clare Peckitt ◽  
Judith Bliss ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vitamin D ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Vitamin D Receptor ◽  
Caucasian Population ◽  
25 Hydroxy Vitamin D

scholarly journals Differential expression of Vitamin D binding protein in thyroid cancer health disparities

Oncotarget ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 596-607
Author(s):  
Brittany Mull ◽  
Ryan Davis ◽  
Iqbal Munir ◽  
Mia C. Perez ◽  
Alfred A. Simental ◽  
...  
Keyword(s):  
Vitamin D ◽  
Health Disparities ◽  
Thyroid Cancer ◽  
Differential Expression ◽  
Binding Protein ◽  
Vitamin D Binding Protein ◽  
Cancer Health Disparities
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