scholarly journals Rapid detection and identification of dioscorine compounds in Dioscorea hispida tuber plants by LC-ESI-MS

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 5999-6011
Author(s):  
Zatil Hazrati Kamaruddin ◽  
S. M. Sapuan ◽  
Mohd Zuhri Mohamed Yusoff ◽  
Ridhwan Jumaidin
Keyword(s):  
Relative Abundance ◽  
Rapid Detection ◽  
Water Solution ◽  
Peninsular Malaysia ◽  
Ionization Mass ◽  
Distilled Water ◽  
Nacl Solution ◽  
Esi Ms ◽  
Tropical Area ◽  
Detection And Identification

Studies have revealed that Dioscorea hispida tubers contain a poisonous substance called alkaloid dioscorine. The method for removing alkaloid dioscorine in Dioscorea hispida is explored in this research through a soaking process. The tubers were peeled, washed, sliced, and soaked for 5 days in either 1.0 M sodium chloride (NaCl) or distilled water. The aim of this study was to firstly identify the amount of toxic dioscorine remaining after soaking for 5 days, and then determine the best solution for removing dioscorine compounds in the tubers that were obtained from a tropical area in Peninsular Malaysia. The liquid chromatography electrospray ionization mass spectrometric (LC-ESI-MS) systems were used to identify the presence of alkaloid dioscorine compounds within Dioscorea hispida tubers. The analysis showed that no dioscorine compounds were present in day 5 for samples soaked in the NaCl solution. However, the relative abundance in the distilled water solution at day 5 was 281000, indicating a 95% decrease of the relative abundance value of the dioscorine compounds. This work aimed to determine the minimum days needed to remove the poisonous element before Dioscorea hispida tubers could be used for food consumption or for any other applications.

scholarly journals Abberant Immunoglobulin G Glycosylation in Rheumatoid Arthritis by LTQ-ESI-MS

2020 ◽  
Vol 21 (6) ◽  
pp. 2045 ◽  
Author(s):  
Zhipeng Su ◽  
Qing Xie ◽  
Yanping Wang ◽  
Yunsen Li
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immunoglobulin G ◽  
Ion Trap ◽  
Ionization Mass ◽  
Healthy Controls ◽  
Esi Ms ◽  
Aberrant Glycosylation ◽  
Detection And Identification ◽  
Highly Sensitive

Aberrant glycosylation has been observed in many autoimmune diseases. For example, aberrant glycosylation of immunoglobulin G (IgG) has been implicated in rheumatoid arthritis (RA) pathogenesis. The aim of this study is to investigate IgG glycosylation and whether there is an association with rheumatoid factor levels in the serum of RA patients. We detected permethylated N-glycans of the IgG obtained in serum from 44 RA patients and 30 healthy controls using linear ion-trap electrospray ionization mass spectrometry (LTQ-ESI-MS), a highly sensitive and efficient approach in the detection and identification of N-glycans profiles. IgG N-glycosylation and rheumatoid factor levels were compared in healthy controls and RA patients. Our results suggested that total IgG purified from serum of RA patients shows significantly lower galactosylation (p = 0.0012), lower sialylation (p < 0.0001) and higher fucosylation (p = 0.0063) levels compared with healthy controls. We observed a positive correlation between aberrant N-glycosylation and rheumatoid factor level in the RA patients. In conclusion, we identified aberrant glycosylation of IgG in the serum of RA patients and its association with elevated levels of rheumatoid factor.

scholarly journals Interactions between hydro-soluble degradation products from a radio-oxidized polyesterurethane and Eu(III) in contexts of repositories for low and intermediate level radioactive waste

2020 ◽  
Vol 108 (5) ◽  
pp. 383-395 ◽  
Author(s):  
Elodie Fromentin ◽  
Diane Lebeau ◽  
Alexandre Bergounioux ◽  
Muriel Ferry ◽  
Pascal E. Reiller
Keyword(s):  
Degradation Products ◽  
Water Solution ◽  
Gas Chromatography Mass Spectrometry ◽  
Luminescence Spectroscopy ◽  
Ionization Mass ◽  
Solution Ph ◽  
Time Resolved ◽  
Dioic Acid ◽  
Esi Ms ◽  
Complexation Reactions

AbstractThe complexation of Eu(III) by hydro-soluble degradation products (HDPs) from a radio-oxidized polyesterurethane is investigated. The polyesterurethane Estane 5703® (PURe) is radio-oxidized at 1000 kGy with γ-rays at room temperature. The polymer is then hydrolysed by a simplified artificial cement pore water solution (pH 13.3) for 31 days at 60 °C. The HDPs within the leachate are characterized using gas chromatography-mass spectrometry, ionic chromatography, and TOC analyser. The complexation of Eu(III) is studied by time-resolved luminescence spectroscopy (TRLS). The main HDPs are adipic acid – hexane-1,6-dioic acid – and butane-1,4-diol. Unlike HDPs from non-irradiated PURe, the HDPs from 1000 kGy γ-irradiated PURe do form complexes with Eu(III) at pH 13.3. Neither adipate nor butane-1,4-diol are responsible for this complexation. The existence of several types of complexes is evidenced by TRLS and electrospray ionization mass spectroscopy (ESI-MS): complexation reactions and operational constants are proposed. The complexes formed at high pH (from 10 to 13) are different from the lower pH complexes. The lower pH complexes are studied by ESI-MS and two ligands are identified: adipate and an oligomer.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

scholarly journals A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B1 in apples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhenlong Guo ◽  
YiFei Su ◽  
Kexin Li ◽  
MengYi Tang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Limit Of Detection ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Ionization Mass ◽  
Limit Of Quantification ◽  
Visible Absorption ◽  
Esi Ms ◽  
Relative Standard ◽  
Ft Ir

AbstractThe development of detecting residual level of abamectin B1 in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5′-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B1 in apples. The TPB molecule has been characterized by ultraviolet–visible absorption spectrometry, 1H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectra. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L−1 and 4.4 μg L−1, respectively. The mechanism of this probe to identify abamectin B1 was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B1 in apples was achieved. Good reproducibility was present based on relative standard deviation of 2.2%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B1 of this fluorescent probe demonstrated reasonable sensitivity, specificity and selectivity. The results indicate that the octopus-like azobenzene fluorescent probe can be expected to be reliable for evaluating abamectin B1 in agricultural foods.

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