Formation of potato microtubers in vitro depending on temperature and light intensity

2016 ◽  
Vol 3 (3) ◽  
pp. 73-78
Author(s):  
Yu. Lavrynenko ◽  
R. Vozhegova ◽  
H. Balashova ◽  
O. Kotova ◽  
B. Kotov
Keyword(s):  
Light Intensity ◽  
Test Tube ◽  
Tuber Formation ◽  
Meristem Culture ◽  
Systematic Analysis ◽  
In Vitro Methods ◽  
Effi Ciency ◽  
Early Maturing ◽  
The Cost

Aim. To specify optimal techniques infl uencing the increase of intensity of potato tuber formation in meristem culture in vitro. Methods. Integrated use of laboratory, mathematical and statistical, calculation and compari- son methods and the method of systematic analysis. Results. The paper represents experimental data about the infl uence of temperature and light intensity on the induction of tuber formation under microclonal reproduction of the health-improved initial material. It proves that the decisive factor in the process of morphogenesis of plants in vitro of the early maturing potato variety Kobza and their productivity is temperature conditions for cultivation. Conclusions. Optimal indexes of productivity and economic effi ciency are maintained by grow- ing test-tube plants under the temperature of cultivation of 14–16 oС and the light intensity of 3,000 lux. The number of microtubers per plant was 1.2 pieces, the weight of an average microtuber was 262,0 mg, the weight of microtubers per plant was 363,7 mg, the number of microtubers weighing more than 350,0 mg was 20,9 %; the cost price of microtubers was 5,31 UAH with the profi tability of 201 %.

scholarly journals Formation of potato microtubers in vitro depending on temperature and light intensity

1970 ◽  
Vol 21 ◽  
pp. 57-61
Author(s):  
Yu. O. Lavrynenko ◽  
H. S. Balashova ◽  
V. V. Bazalii
Keyword(s):  
Light Intensity ◽  
Test Tube ◽  
Tuber Formation ◽  
Meristem Culture ◽  
Systematic Analysis ◽  
In Vitro Methods ◽  
Early Maturing ◽  
Temperature Mode ◽  
The Cost

Aim. To specify optimal techniques influencing the increase of intensity of potato tuber formation in meristem culture in vitro. Methods. Integrated use of laboratory, mathematical and statistical, calculation and comparison methods and the method of systematic analysis. Results. The paper represents experimental data about the influence of temperature and light intensity on the induction of tuber formation under microclonal reproduction of the health-improved initial material. It proves that the decisive factor in the process of morphogenesis of plants in vitro of the early maturing potato variety Kobza and their productivity is temperature conditions for cultivation. Conclusions. Optimal indexes of productivity and economic efficiency are maintained by growing test-tube plants under the temperature of cultivation of 14–16ºC and the light intensity of 3000 lux. The number of microtubers per plant was 1.2 pieces, the weight of an average microtuber was 262.0 mg, the weight of microtubers per plant was 363.7 mg, the number of microtubers weighing more than 350.0 mg was 20.9 %; the cost price of microtubers was 5.31 UAH with the profitability of 201 %. Keywords: potato, microtubers, temperature mode, light intensity, in vitro.

scholarly journals Use of growth inhibitor chlormequat chloride in potato culture in vitro

2021 ◽  
Vol 285 ◽  
pp. 03003
Author(s):  
Sergey A. Buldakov
Keyword(s):  
Nutrient Medium ◽  
Growth Inhibitor ◽  
Test Tube ◽  
Investigational Product ◽  
Chlormequat Chloride ◽  
Skoog Medium ◽  
Potato Culture ◽  
Murashige And Skoog Medium ◽  
The Cost

The publication presents data on the use of growth inhibitor chlormequat chloride in in vitro potato culture on varieties of different maturity groups: Meteor (early), Zekura (mid-early), and Northern Lights (mid-season). Five dosages of the investigational product were studied, ranging from 0.1125 to 1.8 g/l. It was found that in maximum doses of the product there was a strong inhibition of all growth processes in all varieties. The research results showed that the most optimal concentration of chlormequat chloride is 0.225 g per 1 liter of Murashige and Skoog medium. At this dose, on 30th day of cultivation, there was a decrease in the height of microplants from the control by 63.2-85.1%, in the root length - up to 15.0% and their number - up to 22.8% and an increase in the number of internodes by 6.5-22,0 % depending on the variety. The investigational product had an effect on formation of microtubers; in the Meteor variety, their largest number was 89.5% in the nutrient medium with a dose of 1.8 g/l, in the Zekura variety - 93.0% in the nutrient medium with a dose of 0.9 g/l. The new technique makes it possible to lengthen the periods between cuttings of test-tube plants by 2.3 times. This, in turn, reduces the cost of maintaining the in vitro collection material of potatoes in the summer-autumn period, and improves its quality, since each additional cutting cycle affects a more rapid degeneration of the variety. Also, microplants grown with growth inhibitor during subsequent relocation to a standard Murashige and Skoog medium did not show an aftereffect.

Plant Morphogenesis under Different Light Intensity

2018 ◽  
Vol 386 ◽  
pp. 201-206 ◽  
Author(s):  
Yuriy Nikolaevich Kulchin ◽  
Olga Valerievna Nakonechnaya ◽  
Irina Victorovna Gafitskaya ◽  
Olga Vadimovna Grishchenko ◽  
Tatyana Yuryevna Epifanova ◽  
...  
Keyword(s):  
Light Intensity ◽  
Stevia Rebaudiana ◽  
Illumination Intensity ◽  
Soil Conditions ◽  
Light Sources ◽  
Cultivation Time ◽  
Optimal Values ◽  
The Cost ◽  
Fluorescent Lighting

The innovative LED light source (Sun Box) with irradiation spectrum close to the sun spectrum in the wavelength range 440-660 nm was used in experiment for study the influence of light intensity (75, 135, 230 and 382 μmol/s*m2) on the growth and development of plants. Standard fluorescent lighting was used as a control. The experiments were carried out on plantlets ofStevia rebaudianaandSolanum tuberosum, cvs. Snegir, Rozhdestvenskiy and Kamchatskii)in vitro. The illumination intensity of 75 and 230 μmol/s*m2promoted development ofS. rebaudianaplantlets with optimal values of morphometric parameters and well developed roots, which is important for plantlet adaptation to soil conditions. ForS. tuberosumplantlets (Snegir and Rozhdestvenskiy cultivars), radiation intensity of 135 μmol/s*m2was optimal for micropropagation. The illumination intensity of 230 μmol/s*m2led to a formation of plantlets with the largest total fresh mass among experimental groups. Sun Box light with intensity of 75 μmol/s*m2could be applicated for micropropagation of these cultivars: plantlets were the highest with the largest internodes number. Thus, the plant response to different light intensity was species-spesific, and – in case of potato plantlets – cultivar-spesific. The use of artificial light sources with distinct PPFD level could be preferable forS. tuberosumandS. rebaudianaplantlet micropropagationin vitro, as it could shorten the cultivation time, accelerate cultivation time, and reduce the cost of electricity.

Methods of intensifying the tuberization process of potato seeds in vitro culture

2017 ◽  
Vol 4 (2) ◽  
pp. 37-41
Author(s):  
H. Balashova ◽  
Yu. Lavrynenko ◽  
R. Vozhehova ◽  
B. Kotov
Keyword(s):  
Experimental Data ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Early Potato ◽  
Medium Ph ◽  
In Vitro Methods ◽  
Systemic Analysis ◽  
Effi Ciency ◽  
The Impact

Aim. To determine optimal factors of impacting the tuberization intensity of the plants of mid-early potato variety Nevska in vitro. Methods. Complex application of the laboratory, mathematical-statistical, calculative- comparative methods and systemic analysis. Results. The experimental data on the impact of photoperiod and acidity of the culture medium (pH) on the tuberization induction in culture in vitro were presented. The dependence of tuberization intensity on pH of the culture medium was demonstrated. Conclusions. Optimal indices of tuberization intensity and economic effi ciency were obtained during in vitro cultivation of the plants of mid-early Nevska variety at the photoperiod of 16 hours and pH of the culture medium of 5.3: tuberization intensity was 83.0 %, the mass of the average microtuber was 612.5 mg, the mass of microtubers per one plant was 514.4 mg, the number of microtubers with the mass over 350 mg – 75.4 % with the production profi tability of 100 % and microtuber cost price of UAH 8.08 (USD 0.3).

A Comparison of the Test Tube and the Dialysis Tubing in Vitro Methods for Estimating the Bioavailability of Phosphorus in Feed Ingredients for Swine

2005 ◽  
Vol 53 (9) ◽  
pp. 3287-3294 ◽  
Author(s):  
David W. Bollinger ◽  
Atsushi Tsunoda ◽  
David R. Ledoux ◽  
Mark R. Ellersieck ◽  
Trygve L. Veum
Keyword(s):  
Test Tube ◽  
Dialysis Tubing ◽  
Feed Ingredients ◽  
In Vitro Methods

Anti-amnesic activity screening of the seed ethanol extracts of Turkish Paeonia taxa by in vitro methods

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
D Sevim ◽  
FS Senol ◽  
I Orhan ◽  
B Şener ◽  
E Kaya
Keyword(s):  
In Vitro Methods ◽  
Activity Screening ◽  
Ethanol Extracts

Analysis of antidepressant properties of some Papaver species by in vivo and in vitro methods

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
OML Bayazeid ◽  
F Yalcin ◽  
M İlhan ◽  
H Karahan ◽  
E Kupeli-Akkol ◽  
...  
Keyword(s):  
In Vitro Methods

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Effects of Some Synthetic Compounds on In Vitro Fibrinolytic Activity Measured by Different Methods and the Relevance to Activity In Vivo

1972 ◽  
Vol 28 (03) ◽  
pp. 351-358
Author(s):  
A.J Baillie ◽  
A. K Sim
Keyword(s):  
Inhibitory Activity ◽  
Substrate Concentration ◽  
Fibrinolytic Activity ◽  
Synthetic Compounds ◽  
In Vitro Methods ◽  
Narrow Concentration Range

SummaryThe activity of several synthetic compounds, rated from good to poor (or inactive) fibrinolytic activators, has been assessed by two different commonly-used in vitro methods. Compounds shown to be active over a narrow concentration range in the hanging clot test were shown to be inhibitors of plasmin and trypsin in the casein-olytic test. The inhibitory activity of these compounds was shown to increase with increasing substrate concentration and apparent activity in the hanging clot test. Possible explanations and relevance of these observations are discussed.

ECONOMIC PERSPECTIVES OF BIOTECHNOLOGIES USING IN ANIMAL FARMING

1970 ◽  
pp. 57-60
Author(s):  
I. F. Gorlov ◽  
A. A. Mosolov ◽  
G. V. Komlatskiy ◽  
M. A. Nesterenko ◽  
K. D. Nimbona ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Economic Effect ◽  
Dairy Herd ◽  
State Level ◽  
Modern Level ◽  
Economic Perspectives ◽  
The Cost ◽  
Short Time

The article presents materials on the study of the possibility of reproduction and increase in the herd of highly productive cows through the use of embryo transplantation technology. The classical (in vivo) and more modern, developing (in vitro) methods of embryotransfer, their positive and negative sides are considered in detail. The possibility of accelerating the breeding process by using the method of transplantation, in which from one cow can be obtained from 10 to 100 calves, which will allow for 4-5 years, almost any herd (of any size and breed) with the help of biotechnology to turn into a cattle-breeding enterprise of the most modern level. At the same time, heifers obtained from unproductive cows can be used as "surrogate" mothers who are transplanted with the best donor embryos, which allows to obtain a full-fledged offspring adapted to local environmental conditions. A detailed scheme of obtaining, evaluation, storage, as well as the cost and economic effect of embryo transplantation was calculated, the market was evaluated, the required annual volume of transplants and the number of donor cows for large livestock farms were determined. As a positive example of "Scientific-production enterprise "Centre of biotechnology and embryo transfer" in 2014, implemented a project for accelerated replacement and genetic improvement of the dairy herd, engraftment averaged 57-69%, and the economic effect of the enterprise from getting a single animal by the method of embryo transfer, compared with imports of similar close in quality, ranged from 60 to 100 thousand rubles on his head. It is shown that it is necessary to organize at the state level a developed service for embryo transplantation to reduce the cost of embryo transfer and the possibility of creating in a short time in the country's own highly productive breeding nucleus of dairy and beef cattle, which will reduce, and in the future completely eliminate, import dependence on cattle products.

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