Intra- and interbreed genetic heterogeneity and divergence in four commercial pig breeds based on microsatellite markers

In recent years, there has been an increasing amount of attention paid to the genetic health of domesticated animals and its relationship with the level of inbreeding and genetic diversity. At the same time, insufficient attention is still paid to the study of intrabreed genetic diversity and intrabreed stratification. The main goal of our work was to analyze the intra- and interbreed genetic diversity of commercial pig breeds on the basis of DNA microsatellite (MS-DNA) polymorphism. In total, the work used data for 3,308 pigs, which represented 11 herds. The animals belonged to four commercial pig breeds – Duroc (DR), Yorkshire (YR), Landrace (LN) and Large White (LW). 12 microsatellite loci recommended by ISAG-FAO and arranged in one multiplex panel (S0101, S0155, S0228, S0355, S0386, SW24, SW240, SW72, SW857, SW911, SW936, SW951) were used as DNA markers. When analyzing the intra- and interbreed variability of 11 herds, we found that all studied breeds significantly differed in terms of the proportion of both rare and the most common alleles. At the same time, the noted differences were determined, first of all, by the variability between individual herds within their breed. The location of herd centroids is random and is not consistent with their breed affiliation at all. When individuals belonging to the same breed are combined, the centroids of pig breeds in the space of first two axes from a Principal Coordinate Analysis form two clusters. The first one contains the only red pig breed (DR) used in the analysis, while the second one contains white pig breeds. In six pig herds the Ne estimates were below 50 inds., in two herds they were in the range of 50–100 inds., and finally in three herds the Ne estimates exceeded 100 inds. The analysis of the genetic variability of pigs of four commercial breeds showed that the high level of interbreed differences is caused, first of all, by the high variability among pig herds within each studied breed. Such intrabreed stratification can be formed due to the manifestation of many causes: different genetic basis of the founders of intrabreed genealogical groups, geographical isolation, different directions of selection within individual herds, exchange of animals between separate herds, the use of inbreeding in the practice of selection together with isolation, etc. Important consequences of intrabreed stratification are an increase in the level of interherd diversity (which is not lower than the level of interbreed diversity) against the background of a decrease in variability within individual herds, as well as a significant deficit of heterozygotes and an increase in the role of negative genetic and demographic processes. Thus, the existence of genetic heterogeneity within commercial pig breeds should be considered as an essential element in the history of their formation and breeding.

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Phylogeographic pattern of Jenynsia multidentata(Cyprinodontiformes: Anablepidae) in the southern boundary of the Brazilian Subregion, Argentina

Neotropical Ichthyology ◽

10.1590/s1679-62252013000300001 ◽

2013 ◽

Vol 11 (3) ◽

pp. 477-486 ◽

Cited By ~ 2

Author(s):

María Cecilia Bruno ◽

Jorge Rafael Casciotta ◽

Adriana Edith Almirón ◽

Marta Susana Lizarralde

Keyword(s):

Genetic Diversity ◽

Wide Distribution ◽

Geologic History ◽

Drainage Basins ◽

Phylogeographic Pattern ◽

Southern Boundary ◽

Paleoclimatic Changes ◽

Demographic Processes ◽

History Of ◽

Jenynsia Multidentata

The Atlantic drainage basins are located in the southern Pampean area, in the southernmost part of the Brazilian subregion. Tectonic and paleoclimatic phenomena, occurring during the Quaternary, have currently isolated these basins from the present hydrographic system. Their ichthyofaunal composition is similar to that of hydric systems located further northward. Jenynsia multidentata is a species with wide distribution in the Atlantic drainage basins, as well as in those Northern basins. Here we investigated the phylogeographic pattern of Jenynsia multidentataand analyzed its relationships with the paleoclimatic and geologic history of the region. The analysis of the population's assemblage showed moderate genetic diversity, demographic equilibrium and marked genetic structure in the populations that occupy the extremes of the distributional range. The populations in the center of the range did not present genetic diversity, sharing a single haplotype. These results allow inferring that the presence of Jenynsia multidentata in the area results from historical demographic processes that are independent but complementary. In turn, these processes could arise from paleoclimatic changes occurred during the Quaternary.

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Genetic Diversity and Population Structure of Endangered Catfish Rita rita (Hamilton, 1822) Revealed by Heterologous DNA Microsatellite Markers

Asian Fisheries Science ◽

10.33997/j.afs.2021.34.2.007 ◽

2021 ◽

Vol 34 (2) ◽

Author(s):

MUHAMMAD FORHAD ALI ◽

◽

MD. RAFIQUL ISLAM SARDER ◽

MOHAMMAD MATIUR RAHMAN ◽

MD. FAZLUL AWAL MOLLAH ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Gene Diversity ◽

Polymorphic Information Content ◽

Genetic Difference ◽

Information Index ◽

Hardy Weinberg Equilibrium ◽

High Gene ◽

High Level ◽

Dna Microsatellite

Genetic information is essential for conservation and future aquaculture development of the endangered catfish Rita rita (Hamilton, 1822). Two hundred catfish, R. rita, 50 from four rivers, the Old Brahmaputra, Jamuna, Meghna and Kangsa were collected and analysed to evaluate the genetic diversity and population structure using five microsatellite primers (Cba06-KUL, Cba08-KUL, Cba09-KUL, Phy03-KUL and Phy07-KUL). Four of the five amplified loci were found polymorphic (P95) in all the populations and 46 alleles were recorded with 9 to 14 alleles per locus. Differences were observed in the total number of alleles ranging from 41 to 44, effective number of alleles from 29.96 to 37.46, observed heterozygosity from 0.57 to 0.76, Shannon’s information index from 2.09 to 2.30 and polymorphic information content from 0.84 to 0.88 among the four populations. Results exposed the highest levels of genetic diversity in the Meghna population while the lowest in the Kangsa population of R. rita. All the populations were significantly deviated (P < 0.001) from the Hardy-Weinberg equilibrium for all the loci. Nei’s genetic distance between populations ranged 0.007 to 0.017 with low overall genetic difference FST = 0.011 and high gene flow Nm = 24.333, indicating that R. rita populations were not subdivided. This study revealed a high level of gene diversity with deficiency in genetic heterogeneity in all the populations of R. rita, emphasising natural management, conservation and rehabilitation measures of this species.

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The Genetic Structure of Five Pig Breeds Maintained in Poland

Annals of Animal Science ◽

10.1515/aoas-2016-0006 ◽

2016 ◽

Vol 16 (4) ◽

pp. 1019-1027 ◽

Cited By ~ 1

Author(s):

Tomasz Szmatoła ◽

Katarzyna Ropka-Molik ◽

Mirosław Tyra ◽

Katarzyna Piórkowska ◽

Kacper Żukowski ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Information ◽

Allelic Richness ◽

Microsatellite Data ◽

Large White ◽

Model Based Clustering ◽

Pig Breeds ◽

Small Admixture ◽

High Degree ◽

Mcmc Approach

Abstract The aim of this study was to evaluate genetic variability of five pig populations maintained in Poland with the usage of model based clustering method (MCMC approach) on microsatellite data. A high degree of genetic diversity with the values corrected for sample sizes was observed for four breeds: 0.740 for Polish Landrace, 0.697 for Pietrain, 0.692 for Polish Large White and 0.688 for Puławska. Duroc breed, however, was characterized by the lowest genetic diversity (0.589), allelic richness and number of effective alleles. As for Structure software results, each breed was represented by their own cluster while maintaining a possible small admixture of other breeds. The results obtained suggest a moderate gene flow between breeds, especially between Polish Landrace and Polish Large White has occurred. The lowest admixture was presented for Duroc breed, which confirms its high purity. The presented results can be used to study the structure and as a genetic information for the preservation and further genetic improvement of the pig breeds maintained in Poland. It seems also advisable to conduct further studies with a larger number of microsatellites and by analysis of mitochondrial DNA.

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A Confrontation between Two Doctrines: The Birth of Struggle for Hegemony in Hebrew Children's Literature during the 1930s and 1940s

International Research in Children s Literature ◽

10.3366/ircl.2008.0003 ◽

2008 ◽

Vol 1 (2) ◽

pp. 139-155 ◽

Cited By ~ 2

Author(s):

YAEL DARR

Keyword(s):

Children's Literature ◽

Educational System ◽

Single Channel ◽

Children’S Literature ◽

The Political ◽

Literary Creation ◽

History Of ◽

High Level ◽

First Time ◽

Literary Quality

This article describes a crucial and fundamental stage in the transformation of Hebrew children's literature, during the late 1930s and 1940s, from a single channel of expression to a multi-layered polyphony of models and voices. It claims that for the first time in the history of Hebrew children's literature there took place a doctrinal confrontation between two groups of taste-makers. The article outlines the pedagogical and ideological designs of traditionalist Zionist educators, and suggests how these were challenged by a group of prominent writers of adult poetry, members of the Modernist movement. These writers, it is argued, advocated autonomous literary creation, and insisted on a high level of literary quality. Their intervention not only dramatically changed the repertoire of Hebrew children's literature, but also the rules of literary discourse. The article suggests that, through the Modernists’ polemical efforts, Hebrew children's literature was able to free itself from its position as an apparatus controlled by the political-educational system and to become a dynamic and multi-layered field.

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Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

Agricultural science and practice ◽

10.15407/agrisp3.02.042 ◽

2016 ◽

Vol 3 (2) ◽

pp. 42-48

Author(s):

V. Balatsky ◽

I. Bankovska ◽

A. Saienko

Keyword(s):

Gene Polymorphism ◽

Leptin Receptor ◽

Receptor Gene ◽

Intramuscular Fat ◽

Large White ◽

Pig Breeds ◽

Lepr Gene ◽

Large White Breed ◽

White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

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The way of value of Correlation of genomic DNA microsatellite loci and live weight of Chukchi reindeer

Genetika i razvedenie zhivotnyh ◽

10.31043/2410-2733-2020-3-27-32 ◽

2020 ◽

pp. 27-32

Author(s):

V. Dodokhov ◽

N. Pavlova ◽

T. Rumyantseva ◽

L. Kalashnikova

Keyword(s):

Microsatellite Markers ◽

Agricultural Production ◽

Live Weight ◽

Biodiversity Loss ◽

Tribal Community ◽

High Germination ◽

Wide Range ◽

The Mean ◽

High Level ◽

Dna Microsatellite

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

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CONNECTION g.307 G > A SNP POLYMORPHISM OF THE ALPHA-FUKOZYLTRASFERAZA 1 GENE WITH ECONOMIC-USEFUL TRAITS IN THE LARGE WHITE PIG BREED

Animal Breeding and Genetics ◽

10.31073/abg.54.17 ◽

2017 ◽

Vol 54 ◽

pp. 134-140

Author(s):

H. S. Rudoman ◽

V. M. Balatsky ◽

V. Y. Nor ◽

V. O. Vovk

Keyword(s):

Genetic Structure ◽

Information Content ◽

Analysis Of Variance ◽

Farm Animals ◽

Daily Weight Gain ◽

Fixation Index ◽

Its Sequencing ◽

Large White ◽

Stage Of Development ◽

High Level

One of the top priorities at the present stage of development of pig breeding remains the development of a set of measures aimed at increasing the resistance animals to various diseases, especially – to colibacteriosis .One of the recent and effective approaches to prevent colibacillosis is using markers of selection; it involves pig genotyping by genome locuses. Chosen locuses are associated with animal sensitivity to the disease and selection of the results of genotyping of animals with increased resistance. Due to researches, one of such locuses is alpha-fukozyltrasferаza 1 gene (FUT1). Gene FUT1 is located in chromosome 6. As a result of its sequencing in the swine breeds of Large White and Swedish Landrace, single-nucleotide polymorphism (g.307 G > A SNP) has been detected. AA genotype determines the resistance of animals to colibacteriosis, while AG and GG genotypes are susceptible to this disease. According to the results of previous studies, the positive effect of allele A was determined not only on the resistance of pigs to colibacteriosis, but also on indicators of fattening and meat productivity and on reproductive performance. In Ukraine, the studies of polymorphism FUT1 g.307 G > A SNP were held fragmentedly and only on certain populations of Ukrainian Meat and Large White breed but without establishing its association with the indicators of productivity of pigs. The aim of our work was to study the genetic structure of Ukrainian Large White breeds, type 1 and the establishment of association. g. 307 G > A SNP FUT1 gene with pigs indicators of productivity. For research the 96 samples of hair were used. DNA isolation from samples with biomaterial were carried out using ion exchange resin Chelex-100. Genotyping was performed by PCR-RFLP by method of Jorgensen et al. (2006). Using DNA analysis of this breeds locus FUT1 was determined by genetic structure. Allele frequency of allele G (0,573) and allele A (0,427) was established The distribution of genotype frequencies was not statistically significantly different from the theoretically expected, calculated by the Hardy-Weinberg criterion. Thus, according to the locus of FUT1 g.307 G > A, the breeds investigated are in a state close to the genetic equilibrium. The negative value of the fixation index by the locus FUT1 g.307 G > A indicates an excess of heterozygotes in the population, and hence the lack of targeted selection for this marker. The valuation was performed by calculating the PIC (polymorphic information content) – information content of polymorphism marker. In the analyzed pig herd for this marker, the PIC has an average value (0,367), which indicates the high level of polymorphism of the given locus and is favorable for the possibility of conducting a search for links between individual genotypes and indicators of productivity. To establish the association g.307 G > A SNP FUT1 gene with indicators of animal productivity, a one-way analysis of variance of the experimental data was used. Based on the results of a one-way analysis of variance, a significant effect of the genotypes of the FUT1gene (g.1849 G > C) on the indicator of the average daily weight gain (р ≤ 0,001), the thickness of the bacon at the level of the VI-VII vertebrae (p ≤ 0.01), reaching live weight of 100 kg (р ≤ 0,001) and breeding index of fattening qualities (р ≤ 0,01) was found. The parameter of the influence of the genetic factor on the test feature was 28,54%, 10,4%, 33,4% and 8,8% respectively. According to the investigated indicators of productivity, animals with genotype AA, which determines resistance to colibacteriosis, dominated the animals with genotypes GG and AG. Based on the results of our research and pre-published data, the multiple effect of the g.307 G > A SNP FUT1 gene is observed, which is associated with the indicators of productivity, which again confirms the polygenicity of the quantitative trait loci of farm animals. Taking into account a high level of polymorphism of the investigated gene and reliable associations of genotypes with indicators of productivity found, it can be recommended to carry out breeding of pigs using genetic information of the g.307 G > A SNP gene FUT1.

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A New Step in the Study of the History of the Russian Prosecutor’s Office

Rossijskoe Pravo. Obrazovanie, Praktika, Nauka ◽

10.34076/2410-2709-2019-3-94-98 ◽

2019 ◽

pp. 94-98

Keyword(s):

Russian Federation ◽

Russian Empire ◽

Practical Significance ◽

History Of ◽

The Russian Federation ◽

The Russian Empire ◽

High Level ◽

The University

The paper is a review on the textbook by A. V. Yeremin, «The History of the National Prosecutor’s office» and the anthology «The Prosecutor’s Office of the Russian Empire in the Documents of 1722–1917» (authors: V. V. Lavrov, A. V. Eremin, edited by N. M. Ivanov) published at the St. Petersburg Law Institute (branch) of the University of the Prosecutor’s office of the Russian Federation in 2018. The reviewers emphasize the high relevance and high level of research, their theoretical and practical significance. The textbook and the anthology will help the students increase their legal awareness, expand their horizons.

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“Ukrayinskyi Prapor” (1923—1932s): the Berlin period of the newspaper of the President of the Ukrainian National Council

Proceedings of Research and Scientific Institute for Periodicals ◽

10.37222/2524-0331-2019-9(27)-2 ◽

2019 ◽

pp. 13-33

Author(s):

Nadiia Kulesha

Keyword(s):

National Policy ◽

Editorial Staff ◽

National Council ◽

Soviet Rule ◽

Editorial Content ◽

Paper Record ◽

Science And Culture ◽

History Of ◽

High Level ◽

Mainstream Press

The centenary of the Ukrainian Revolution (1917―1921s) made relevant the interest to the developments and the personalities of that time, specifically, to the personality of the President of the ZUNR, Petrushevych, Yevhen. The newspaper «Ukrayinskyi Prapor» founded in 1919 in Vienna, throughout its existence, was considered as an official print organ of the Dictator (i.e., Y. Petrushevych). The Vienna period of this publication lasted from August 1919 to mid-November 1923. From the end of November 1923 till April 1932, the paper was published in the capital of the Weimar Republic, Berlin. It was the only newspaper of the Ukrainian emigration published for the longest time in interwar Germany. It was an example of a socio-political periodical. There collaborated outstanding editors and publicists. The pages of this paper record the history of the diplomatic struggle of the West Ukrainian foreign representatives for the liberation of the Eastern Galicia from the protectorate of Poland and the restoration of Ukrainian statehood. Its materials documented the course of the occupation of the Eastern Galicia by Poland and the process of «Polonization» of the Ukrainian population of that region. The article explores the Berlin period of existence of the magazine. Specifically, it studies the changes in the ideological line of the magazine, more specifically, its pro-Soviet editorial orientation because of the illusions about the transformation of the national policy of the Soviet rule in Ukraine, especially during the period of Ukrainization. Then the traditional headings of the magazine were joined by the publications with positive coverage of the flourishing Ukrainianization in Soviet Ukraine. The newspaper also actively reacted to the SVU (Union for Liberation of Ukraine) trial in Kharkiv, justifying the position of the Soviet authorities. The paper’s editorial staff were well-known figures of Ukrainian politics, science, and culture: Yu. Bachynsky, O. Hrytsai, A. Zhuk, M. Lozynsky, R. Perfetsky, and others. They provided a high level of editorial content with high-quality, multifaceted texts. We conclude that in terms of the editorial content and formal aspects, the newspaper «Ukrayinskyi Prapor» matched the standards of the European mainstream press of that time.

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