scholarly journals Disinvasive action of aldehyde and chlorine disinfectants on the test-culture of Toxocara canis eggs

2020 ◽  
Vol 10 (4) ◽  
pp. 175-183 ◽  
Author(s):  
A.P. Paliy ◽  
N.V. Sumakova ◽  
K.O. Rodionova
Keyword(s):  
Nonionic Surfactants ◽  
Consumption Rate ◽  
Sandy Loam ◽  
Effective Means ◽  
Toxocara Canis ◽  
Environmental Objects ◽  
Test Culture ◽  
Soil Disinfection ◽  
Didecyldimethylammonium Chloride ◽  
Test Cultures

Disinfection of environmental objects with highly effective disinfectants is a reliable and effective means of preventing the occurrence of outbreaks of infectious and parasitic diseases. The purpose of our work was to determine the disinfection properties of modern disinfectants based on the test culture of Toxocara canis helminths and to establish the optimal modes of their use. It has been proven that an aldehyde disinfectant containing didecyldimethylammonium chloride (2.25%), benzalkonium chloride (8.0%), glutaraldehyde (15.0%), phosphoric acid, nonionic surfactants, water exhibits disinvasive activity against test cultures of Toxocara canis eggs at a concentration of 2.0-4.0% at a temperature of 20±0.5°C and an exposure of 3-24 hours, and the ovocidal efficiency is from 90.60% to 99.70%. Aldehyde disinfectant can be used for disinfection of soil (black earth, sandy loam, loamy) contaminated with Toxocara canis eggs, at a concentration of 4.0% at 6:00 exposure and a consumption rate of 3000 cm3/m2. Chlorine agent, contains dichlorantin, dimethylhydantoin (12.4-16.4%), dispersant (9.0-12%), nonionic surfactants, corrosion inhibitor, filler exhibits disinvasive activity against Toxocara canis test culture in a concentration of 3.0-4.0% with an exposure of 3-24 hours, and the ovocidal efficiency in this case ranges from 97.40% to 98.82%. The chlorine agent is effective for soil disinfection only at a depth of up to 2 cm at a concentration of 4.0% at a consumption rate of 1000 cm3/m2 and an exposure time of 24 hours.

APPLICATION OF THE DISINFECTANT «ANOLYTE ANK-SUPER» FOR DISINFECTION OF SLAUGHTERHOUSES AND PRIMARY PROCESSING OF CATTLE

2018 ◽  
Vol 1 (1) ◽  
pp. 38-43
Author(s):  
M. P. Butko ◽  
◽  
P. A. Popov ◽  
S. V. Lemyaseva ◽  
D. A. Onischenko ◽  
...  
Keyword(s):  
Consumption Rate ◽  
Drug Consumption ◽  
Meat Processing ◽  
Laboratory Studies ◽  
Primary Processing ◽  
E Coli ◽  
Test Culture ◽  
Processing Plants ◽  
Positive Effect ◽  
Test Cultures

The article presents the results of laboratory studies on the development of disinfection regimes of the slaughterhouses and primary processing of cattle and the testing results of their efficiency in production conditions. The results of the research and production tests established that Anolyte ANK-super provides 100% disinfection of premises of slaughter and primary processing of cattle at meat processing plants and slaughterhouses. So determined, that the positive effect of disinfection of surfaces under control of the test cultures S. aureus and E. coli was achieved at the drug consumption rate 0,3…0,5 l/m2 and exposition of 90 minutes and under control of the test culture of B. cereus at the drug consumption rate of 0,5 l/m2, but exposition was 120 minutes. Materials of the conducted research allowed us to develop the «Technology of the disinfectant Anolyte ANK-super for veterinary-sanitary treatment of premises of slaughter and primary processing of cattle at meat processing plants and slaughterhouses».

scholarly journals Эффективность применения селенитов в период вегетации для обогащения чеснока селеном

2020 ◽  
Author(s):  
А.V. Polykov ◽  
T.V. Alekseeva
Keyword(s):  
Consumption Rate ◽  
Sandy Loam ◽  
Leaf Growth ◽  
Humus Content ◽  
Weather Conditions ◽  
Daily Consumption ◽  
Open Ground ◽  
Loam Soil ◽  
Sodium And Potassium ◽  
Root Treatment

Цель исследования усовершенствование способа обогащения растений чеснока селеном до уровня суточной нормы потребления путем обработки вегетирующих растений растворами селенитов натрия и калия в сочетании с поверхностно-активными веществами и диметилсульфоксидом. Исследования проведены во ВНИИО филиале ФГБНУ ФНЦО, в условиях открытого грунта в 20162018 годах, на супесчаной почве, содержание гумуса в которой составляло 3,413,44, рНKCl 6,7, степень обеспеченности питательными веществами: фосфором высокая (содержание P2O5 в слое 020 см составляло 22,7824,62 мг (по Чирикову) калием низкая, содержание К2О в слое 20 см 10,3817,88 мг (по Масловой). Для улучшения минерального питания растений осенью под основную обработку в почву вносили калий хлористый в дозе 50 г/м2, в период вегетации проводили подкормки растений: первую, в фазе начала интенсивного роста листьев аммиачной селитрой 30 г/м2, вторую, через две недели после первой сульфатом калия 50 г/м2. Погодные условия в течение периода исследований были благоприятны для выращивания чеснока. Поливы проводили по мере подсыхания верхнего слоя почвы. Исследования проведены на чесноке озимом сорта Гладиатор. Схема опытов предполагала предпосадочное замачивание зубков в течение 30 мин. и последующую двукратную обработку вегетирующих растений 0,1-ным раствором селенита натрия и калия в сочетании с поверхностно активными веществами (1) и диметилсульфоксидом (1): первый раз в фазе начала интенсивного роста листьев и второй через три недели. Установлено, что некорневая обработка растений селенитом натрия была в 2,3 раза, а селенитом калия в 5,8 раза эффективнее, чем корневая. Сделан вывод о том, что луковицы чеснока, полученные, в результате некорневой обработки растений 0,1-ным раствором селенита натрия и калия в сочетании с поверхностно активными веществами и диметилсульфоксидом содержат в 145,3169,8 раза больше селена, чем контроль, что позволяет получить зубки, содержащие селен в концентрации 6,837,98 мг/кг. Суточная норма потребления чеснока составляет примерно 10 г, средняя потребность в селене составляет от 30 до 70 мкг. Учитывая, что содержание селена в обогащенной продукции составит 68,3 79,8 мкг/10 г чеснока предложенный способ обогащения позволяет в полной мере удовлетворить суточную потребность организма человека в этом микроэлементе.The aim of investigation was to improve the method of enrichment of garlic plants with selenium to the level of daily consumption rate by treatment of vegetative plants with solutions of sodium and potassium selenites in combination with surfactants. The investigations were carried out at ARRIVG branch of FSCVG, in open ground in 20162018, on sandy loam soil with humus content of 3.413.44, pHKCl 6.7, content of phosphorus (P2O5) in the layer 020 cm was 22.7824.62 mg (Chirikov) content of potassium (K2O) 10.3817.88 mg (Maslova). To improve mineral nutrition of plants fall under the basic treatment in the soil was added potassium chloride at a dose of 50 g/m2 during the growing season were plant nutrition: first, in the early phase of intensive growth of leaves ammonium nitrate 30 g/m2, the second, two weeks after the first potassium sulphate and 50 g/m2. Weather conditions during the period of growth were favorable for growing garlic. Watering was carried out at the periods where the top layer of soil was dried. Studies were carried out on variety Gladiator of winter garlic. The experimental design included soaking the cloves for 30 minutes before planting, and subsequent double treatment of vegetating plants with 0.1 solution of sodium and potassium selenite in combination with surfactants (1) and dimethyl sulfoxide (1): the first time in the phase of the beginning of intense leaf growth and the second time after 3 weeks. As a result of the investigations it was found that non-root treatment of plants with solutions containing selenium was more effective than root treatment. Non-root top dressing with sodium selenite was in 2.3 times, and potassium selenite in 5.8 times more effective than root. It is concluded that garlic bulbs obtained as a result of non-root treatment of plants with 0.1 solution of sodium and potassium Selenite in combination with surfactants and dimethylsulfoxide contain 145.3169.8 times more selenium than control, which allows to obtain teeth containing selenium in a concentration of 6.837.98 mg/kg. The daily consumption rate of garlic is approximately 10 g, the average need for selenium element is from 30 to 70 micrograms. Because of content of selenium in enriched products consisted of 68.3-79.8 mcg/10 g of garlic, the proposed method of enrichment allows to fully satisfy the daily need of the human body at this microelement.

LABORATORY TESTS OF DISINFECTANT «MONOCHLORIDE (IODINE CHLORIDE) 2%»

2018 ◽  
Vol 1 (4) ◽  
pp. 27-33
Author(s):  
N. I. Popov ◽  
◽  
A. V. Suvorov ◽  
S. M. Lobanov ◽  
S. A. Michko ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Laboratory Tests ◽  
Consumption Rate ◽  
Laboratory Work ◽  
Laboratory Studies ◽  
E Coli ◽  
Iodine Chloride ◽  
Test Objects ◽  
Test Cultures

The article describes the results of laboratory tests of the effectiveness of the disinfectant Monochloride (Iodine chloride) 2%. Laboratory studies were carried out on test objects and test surfaces contaminated with test cultures of microorganisms, which included museum cultures of E. coli (E. coli 1257), S. aureus 209-P, mycobacteria (pcs. B5), and spores (B. cereus pieces. 96). Disinfection of test objects was carried out by the method of irrigation at a consumption rate of 0,25...0,3 l/ m2 with disinfection of smooth surfaces and 0,5 l/m2, with disinfection of rough surfaces. The treatment was performed twice with an interval of 60 minutes. Our work established that the Monochloride (Iodine chloride) 2% has a high disinfectant activity against Escherichia coli, Staphylococcus aureus, mycobacteria and spores. On the basis of the laboratory work, this tool can be recommended for production tests at veterinary surveillance facilities.

scholarly journals Synthesis and Antimicrobial Activity of 4-Arylthio- and 4 Alkylthiofunctionalized Pyrazolo[1,5-a]pyrazines

2019 ◽  
Vol 85 (1) ◽  
pp. 58-66
Author(s):  
Yevhenii Hrynyshyn ◽  
Hanna Musiichuk ◽  
Olena Komarovska-Porokhnyavets ◽  
Oksana Is’kiv ◽  
Nataliia Moskalenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Mass Spectra ◽  
Structural Modification ◽  
Bromoacetic Acid ◽  
Phosphorus Pentasulfide ◽  
First Case ◽  
Test Culture ◽  
Exocyclic Methylene ◽  
Derivatives Of ◽  
Test Cultures

The reaction of pyrazolo[1,5-a]pyrazine-4(5H)ones with phosphorus tribromoxide in boiling benzene yielded 4-bromopyrazolo[1,5-a]pyrazines, and the thionation with phosphorus pentasulfide in pyridine at 90 °C led to pyrazolo[1,5-a]pyrazine-4(5H)thiones. The synthesized bromine derivatives are electrophilic, and thiones are nucleophilic substrates. Their subsequent structural modification in the first case was carried out by interaction with thiophenols, and in the second case was conducted with functional halogenoalkanes. It was shown that bromides react with substituted thiophenols in dimethylformamide in the presence of potassium carbonate at 90 °C to form 4-arylthiopyrazolo[1,5-a]pyrazines with yields of 65–83 %. 4-S-methyl-functionalized derivatives of pyrazole[1,5-a]pyrazines with yields of 60–78 % were easily obtained by the alkylation of pyrazole[1,5-a]pyrazin-4(5H)thiones with a-bromoketones, bromoacetic acid, ethyl bromoacetate and bromoacetonitrile in the K2CO3—DMF system at room temperature. The composition of all synthesized compounds is in agreement with the results of elemental analysis and mass spectra. Their structure is confirmed by NMR 1H and 13C spectra. In particular, in the NMR 1H spectra of 4-arylthiopyrazolo[1,5-a]pyrazines, in addition to the characteristic signals of the pyrazole and pyrazine nuclei, signals of protons of thioaryl substituents are present in the range of 7.04 –8.05 ppm, and in NMR spectra of the 1H 4-S-methylfunctionalized derivatives of pyrazole[1,5-a]pyrazines signals of exocyclic methylene protons are present at 4.11– 5.02 ppm. Promising derivatives with antibacterial activity against the test cultures S. aureus (MIC = 7.8 g/mL), M. luteum (MIC = 3.9 g/mL), and antifungal activity against the test culture of fungus A. niger (MIC = 7.8 g/mL) were determined among 4-S-substituted pyrazole[1,5-a]pyrazines as a result of studies of the antimicrobial activity.

EFFECTISAN FOR DISINFECTION OF OBJECTS OF VETERINARY SUPERVISION

2018 ◽  
Vol 1 (2) ◽  
pp. 36-41
Author(s):  
D. I. Udavliev ◽  
◽  
A. M. Abdullaeva ◽  
S. S. Shikhov ◽  
N. E. Vanner ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Quality Control ◽  
Mycobacterium Tuberculosis ◽  
Consumption Rate ◽  
High Antimicrobial Activity ◽  
E Coli ◽  
Selection Of Cultures ◽  
Selection Of ◽  
Test Cultures

The article provides information about the effectiveness of the drug is Effectisan in the laboratory. As a result of the conducted researches it is established that the tested preparation possesses a certain high antimicrobial activity. The drug (in vitro) is effective at concentrations from 0.2 to 1.0% against E. coli (pcs 1257), St. aureus (pcs 209-P), Mycobacterium B-5, Bac. cereus (pcs 96). With wet disinfection at a concentration of 0.5% and a consumption rate of 350 ml/m2, the drug has a pronounced disinfection activity against surfaces made of various materials, the quality control of disinfection in which is carried out by the presence or absence of growth in the washouts of test cultures E. coli, S. aureus. At a concentration of 2.0% and the rate of 350 ml/m2 Effectisan has a pronounced disinfectant activity during processing objects and for quality control of disinfection by selection of cultures of Mycobacterium tuberculosis (pcs-5).

Sanitary parasitological and sanitary–bacteriological condition of the soil in the astrakhan region

2021 ◽  
Vol 23 (3) ◽  
pp. 35-40
Author(s):  
Rudolf S. Arakelian ◽  
Svetlana A. Shemetova ◽  
Olga A. Vanyukova ◽  
Gennadiy L. Shendo ◽  
Nina V. Polyanskaya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Strongyloides Stercoralis ◽  
Toxocara Canis ◽  
Soil Samples ◽  
Coliform Bacteria ◽  
Infected People ◽  
Environmental Objects ◽  
Sanitary Condition ◽  
Eggs And Larvae

This article analyzes the sanitaryparasitological and sanitarybacteriological state of the soil of the Astrakhan region for 2015 to 2019. In total, 7587 soil samples taken from various environmental objects were studied, and 18.197 studies have been performed. Of the samples, 333 (4.4%) samples did not meet the hygiene standards. For parasitic purity, 4566 (60.2%) soil samples were examined, and 9132 (50.2%) studies have been performed. The largest number of soil samples were collected and examined in 2015 and 2016, which amounted to 943 (20.7%) in 2015 and 1046 (22.9%) in 2016. The number of samples that do not meet hygienic standards was 62 (6.6%) in 2015 and 91 (8.7%) in 2016. In 2015, the positive findings were helminthiasis caused by unfertilized eggs of Ascaris lumbricoides (4 [0.5%]), eggs of Toxocara canis (37 [3.9%]), and larvae of Strongyloides stercoralis (21 [2.2%]). For microbiological indicators, the largest number of soil samples were collected and examined in 2019 and amounted to 694 (23.0%), of which positive findings in the form of common coliform bacteria of Escherichia coli amounted to six (0.9%). Studies for bacteriological indicators in 2016 have used 662 (21.9%) samples, which is 0.9 times less than the samples taken in 2019. The sanitary condition of the soil of the Astrakhan region remains stably tense, as evidenced by positive findings in the form of eggs and larvae of helminths, as well as pathogenic bacteria of Escherichia coli. The presence of ascarid eggs, toxocars, and strongylid larvae in the soil indicates contamination of these objects with the feces of infected people and animals. The presence of coliform bacteria of Escherichia coli in the soil also indicates contamination with the feces of sick people.

scholarly journals Disinvasive efficacy of chlorine-based preparations of domestic production for eggs of nematodes of the species Aonchotheca bovis parasitizing in sheep

2018 ◽  
Vol 1 (2) ◽  
pp. 15-18 ◽  
Author(s):  
V. Melnychuk ◽  
I. Yuskiv
Keyword(s):  
Effective Means ◽  
Destructive Effect ◽  
Domestic Production ◽  
Non Invasive ◽  
Invasive Test ◽  
Test Culture ◽  
Nematode Eggs ◽  
Egg Shell ◽  
High Level

The paper presents the results of experimental researches on the determination of the disinvasion efficiency of chlorinated chemical preparations of domestic production of “Brovades-plus”, “Bi-dez” and “Dezsans” of NPF “Brovafarma” (Ukraine). The conducted researches on the basis of the parasitology laboratory found that the studied disinfectants possess disinvasive properties in respect of non-invasive test culture of eggs of nematodes of the species Aonchotheca bovis (son Capillaria bovis) López-Neyra, 1947, isolated from gonads of females of worms. The dissimilar stability of eggs of capillaries to the effect of the tested agents in vitro is proved. The most effective disinfectant in the case of eggs of capillaries of the species A. bovis was the preparation “Dezsan”. The high level of its disinvasive efficacy (DE – 91.14–100.0%) was registered at the application of the preparation in 1.0–2.0% of the exposure concentration for 10–60 minutes. The chemicals “Bi-dez” and “Brovadez-plus” proved to be less effective in the relatively non-invasive test culture of A. bovis eggs. The high level of efficiency (DE –92.41–100.00%) of the “Bi-dez” was achieved with its use at concentrations of 1.5% (exposure of 30 and 60 min) and 2.0% (exposure of 10–60 minutes) The “Brovadez plus” preparation in vitro proved to be the least effective means of non-invasive test culture of nematode eggs of the species A. bovis, since a high level of disinvasive efficacy (DE – 100.00%) was achieved only with the use of 2.0% solution per exposure 10–60 min. It was established that the experimentally tested chlorine-based means of domestic production resulted in the death of eggs in the culture in due to the destructive effect of the components of the preparations on the shell, the caps of eggs of the capillaries, as well as on the germ in the middle of the egg. In experimental cultures treated with the means, the destruction of the shell of the egg in the location of the caps, the exit of morulae outside, the cessation of development or shrinkage of the embryo and deformation of the egg shell were recorded.

scholarly journals Бактерицидні та дезінфікуючі властивості деззасобу «Алкісепт–100»

2017 ◽  
Vol 19 (73) ◽  
pp. 61-65
Author(s):  
O.L. Tishyn ◽  
G.T. Kopijchuk ◽  
R.V. Khomiak ◽  
O.V. Khyrivskyy ◽  
M.M. Danko
Keyword(s):  
Cell Death ◽  
Production Environment ◽  
Protein Activity ◽  
Vegetative Cells ◽  
Spore Form ◽  
E Coli ◽  
Organic Impurities ◽  
Working Solution ◽  
Didecyldimethylammonium Chloride ◽  
Test Cultures

The results of research bactericidal properties of new domestic disinfectant «Alkisept–100» that based on a glutaraldehyde, didecyldimethylammonium chloride, alkyldimethylbenzylammonium chloride and aminotrismethylenephosphonic acid. Was found bactericidal dilution (BD), bactericidal concentration (BC), phenol coefficient (FC) and protein index (PI). Most susceptible to the effect of disinfectant was test-cultures of P. vulgaris, where cell death came on the 10 and 30 minutes exposures at concentrations of 0.26 and 0.18%, respectively. The action of disinfection preparation for gram-positive bacteria S. aureus, vegetative cells of B. subtilis vegetative cells and cultures of S. typhimurium test-cell death drew under these conditions at concentrations of 0.72 and 0.52%, respectively. Gram-negative bacteria E. coli were more susceptible to disinfectant and cell death drew for these exposures at concentrations of 0.52 and 0.37 %, respectively. BD of this disinfectat were 1.96 and 1.4 higher times for E. coli and S. aureus, respectively, from BD phenol, and in the presence of protein activity investigated disinfection preparation is reduced to 2.74 times. The efficacy of disinfection preparation of microorganisms on surfaces in the decontamination of test objects. It is established that test cultures of E. coli and S. aureus (without organic impurities) at 0.1% concentration of disinfectant is ineffective. For spore form of B. subtilis disinfection preparation concentration at 4.0%. Is ineffective At the 1.0–1.5% and higher concentrations is effective for surface treatment of wood, metal and tile that are contaminated with organic substances and objects subject to veterinary controls, and for elimination spore forms of microorganisms at its working concentration of desinfectant should be 5.0% or higher, the exposure in 120 minutes or more. Proved that the disinfectant «Alkisept–100» in a production environment at a concentration of 1.0% by wet surface irrigation facilities for animals and working solution 0.25–0.3 liters per 1 square meter with an exposure of 2 hours, showing high disinfectant properties.

scholarly journals SCREENING OF PHYTOTOXIC EFFECT OF PESTICIDE BI-58 WITH USE OF ALLIUM CEPA L. AND ZEA MAYS L.

2015 ◽  
Vol 4 (2) ◽  
pp. 133-136
Author(s):  
Dilyara Enverovna Emirova
Keyword(s):  
Zea Mays ◽  
Allium Cepa ◽  
Negative Impact ◽  
Test Culture ◽  
Allium Cepa L ◽  
Phytotoxic Effect ◽  
Sprouted Seeds ◽  
Dose Dependent ◽  
Test Plants ◽  
Test Cultures

We carried out the comparative analysis of phytotoxic effect of BI-58 pesticide on morphometric indicators of roots of seeds of Zea mays L. and Allium cepa L. Results of the conducted research showed that BI-58 in the range of the studied concentrations (0,05 - 0,4 ml/l) had the negative impact on sprouts of Allium cepa and Zea mays' seeds which is shown in inhibition of a root gain and viability of seeds. This influence had the expressed dose-dependent character: increase of concentration of the tested preparation caused decrease in the studied indicators in both test cultures. The dose of 0,05 ml/l of phytotoxic action didn't render on sprouts of Allium cepa and Zea mays. It should be noted the concentration of BI-58 recommended for application (0,1 ml/l) had the expressed phytotoxic effect on test plants. It was established sprouts of Zea mays were more sensitive to toxic effect of pesticide, than Allium cepa. On the basis of these data this dose at cultivation of the tested cultures was recommended not to use. The further increase in concentration of BI-58 (0,2 and 0,4 ml/l) caused the interfaced increase of phytotoxic effect on Самарский научный вестник. 2015. № 2(11) 136 Д.Э. Эмирова СКРИНИНГ ФИТОТОКСИЧЕСКОГО ДЕЙСТВИЯ ПЕСТИЦИДА БИ-58 ... the tested cultures. Length of roots of Allium sera decreased by 1,5 times at concentration of 0,2 ml/l and by 40,66% - at decrease in quantity of the sprouted seeds. The increase in concentration of a preparation (0,4 ml/l) caused decrease in length of roots of sprouts of the studied test culture and oppression of their viability. In particular, length of roots of Allium sera in this option of research decreased by 1,8 times in comparison with control, a viability indicator - by 46,16%. Pesticide had similar impact on Zea mays. In particular, at a dose of BI-58 of 0,2 ml/l length of backs of Zea mays decreased by 45,1%, a viability indicator - for 25,3% in comparison with control. The increase in concentration of the studied preparation twice (0,4 ml/l) caused decrease of the morforometric roots indicators for 52,1%, decrease in viability - for 28,8% in comparison with control option.

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