Sanitary parasitological and sanitary–bacteriological condition of the soil in the astrakhan region

2021 ◽  
Vol 23 (3) ◽  
pp. 35-40
Author(s):  
Rudolf S. Arakelian ◽  
Svetlana A. Shemetova ◽  
Olga A. Vanyukova ◽  
Gennadiy L. Shendo ◽  
Nina V. Polyanskaya ◽  
...  

This article analyzes the sanitaryparasitological and sanitarybacteriological state of the soil of the Astrakhan region for 2015 to 2019. In total, 7587 soil samples taken from various environmental objects were studied, and 18.197 studies have been performed. Of the samples, 333 (4.4%) samples did not meet the hygiene standards. For parasitic purity, 4566 (60.2%) soil samples were examined, and 9132 (50.2%) studies have been performed. The largest number of soil samples were collected and examined in 2015 and 2016, which amounted to 943 (20.7%) in 2015 and 1046 (22.9%) in 2016. The number of samples that do not meet hygienic standards was 62 (6.6%) in 2015 and 91 (8.7%) in 2016. In 2015, the positive findings were helminthiasis caused by unfertilized eggs of Ascaris lumbricoides (4 [0.5%]), eggs of Toxocara canis (37 [3.9%]), and larvae of Strongyloides stercoralis (21 [2.2%]). For microbiological indicators, the largest number of soil samples were collected and examined in 2019 and amounted to 694 (23.0%), of which positive findings in the form of common coliform bacteria of Escherichia coli amounted to six (0.9%). Studies for bacteriological indicators in 2016 have used 662 (21.9%) samples, which is 0.9 times less than the samples taken in 2019. The sanitary condition of the soil of the Astrakhan region remains stably tense, as evidenced by positive findings in the form of eggs and larvae of helminths, as well as pathogenic bacteria of Escherichia coli. The presence of ascarid eggs, toxocars, and strongylid larvae in the soil indicates contamination of these objects with the feces of infected people and animals. The presence of coliform bacteria of Escherichia coli in the soil also indicates contamination with the feces of sick people.

2020 ◽  
Vol 76 (4) ◽  
pp. 117-123
Author(s):  
V.A. Irdeeva ◽  
◽  
R.S. Arakelyan ◽  
Kh.M. Galimzyanov ◽  
M.V. Bogdanyunc ◽  
...  

The parasitic contamination of the soil of rural areas of the Astrakhan region for the presence of eggs and larvae of helminths and cysts of pathogenic intestinal protozoa was studied. The highest number of unsatisfactory soil samples was observed in the Limansky district of the Astrakhan region – 12.2 %, when dead larvae of Strongyloides stercoralis and unfertilized eggs of Askaris lumbricoides were found. The presence of Toxocara canis eggs and dead Strongyloides stercoralis larvae in the soil indicates contamination of this object with the feces of invasive animals. The presence of unfertilized eggs of Askaris lumbricoides in soil samples taken from playgrounds in rural areas of the Astrakhan region indicates that these objects are contaminated with the feces of infected people. Contamination of soil in rural areas of the Astrakhan region eggs and larvae of helminths, in the Krasnoyarsk area above 4.1 times higher than in lymans'ke and 6.7 times compared to Kamyzyaksky


Author(s):  
Viktoria Aleksandrovna Irdeeva ◽  
Rudolf Sergeevich Arakelyan ◽  
Gennadiy Leonidovich Shendo ◽  
Nina Vitalievna Polyanskaya ◽  
Tatyana Vasilievna Nikeshina ◽  
...  

The article presents material on the sanitary and parasitological state of environmental objects of the Astrakhan region for 2010–2019 on the example of studying samples of soil, water, manure and flushes from solid household surfaces. A total of 80962 samples were collected and examined during the analyzed period. The number of samples that did not meet the standard indicators was 1.2 % (951 samples). The total share of soil samples in the structure of all studies of environmental objects was 13.2 % (10,692 samples). In addition to the soil, manure samples from farms were studied. In total, 0.04 % (33 samples) of manure were examined during the analyzed period, of which 24.2 % (8 samples) were positive findings in the form of Strongyloides stercoralis larvae. These manure samples were collected only in 2010. The share of water samples was 7.5 % (6033 samples). Also conducted research on samples of swabs taken from household hard surfaces. A total of 64,204 washout samples (79.3 %) were selected and examined, of which 0.02 % (12 samples) were positive. The result of this work were the results: sanitary-parasitological status of water and soil Astrakhan region remain strained, as evidenced by positive findings in the form of eggs and larvae of helminths and cysts of intestinal pathogenic protozoa.


2016 ◽  
Vol 5 (3) ◽  
pp. 157-164
Author(s):  
Wiwid Widyaningsih ◽  
Supriharyono Supriharyono ◽  
Niniek Widyorini

ABSTRAK Kali Wiso merupakan sungai yang berada di tengah kota Jepara. Perairan ini menjadi tempat pembuangan limbah-limbah secara langsung. Limbah tersebut diantaranya limbah domestik, limbah pasar, limbah kapal, serta limbah TPI. Berdasarkan masukan limbah tersebut menjadikan muara ini tercemar. Perairan yang tercemar dapat dilihat dari pengamatan secara fisika, kimia, maupun biologis. Kondisi perairan yang tercemar secara biologis dilihat dari keberadaan bakteri patogen yang ada di perairan. Indikator bakteri yang digunakan yaitu bakteri coliform, karena sifatnya yang berkorelasi positif dengan bakteri patogen lainnya. Pemanfaatan perairan ini digunakan untuk kegiatan pelabuhan, tempat bersandar kapal nelayan, serta kegiatan perikanan yang ada di sekitar perairan Jepara. Oleh karena itu perlu diketahui kepadatan bakteri coliform sehingga dapat bermanfaat sesuai dengan peruntukannya. Tujuan dari penelitian ini adalah untuk mengetahui total bakteri coliform serta mengetahui adanya bakteri Escherichia coli. Penelitain ini dilakukan pada bulan Maret 2016 di Muara Kali Wiso dengan dua kali pengulangan dalam kondisi pasang dan surut. Metode yang digunakan yaitu survei dengan teknik sampling purposive sampling. Metode analisa laboratorium yang digunakan berdasarkan SNI -01-2332-1991. Kepadatan bakteri coliform pada perairan muara Kali Wiso yaitu >110.000 sel/100ml dan bakteri Escherichia coli sebesar >110.000 sel/100ml. Pada kondisi pasang dan surut kepadatan bakteri coliform dan Escherichia coli memiliki nilai perikaraan yang sama, namun tidak menandakan bahwa total bakteri keduanya sama. Kepadatan bakteri coliform dan Escherichia coli telah melebihi batas kriteria mutu air yang telah ditetapkan. Keberadaan bakteri patogen ini bisa mengkontaminasi biota-biota yang ada di perairan. Sehingga jika biota tersebut dikonsumsi oleh manusia bisa menyebabkan gangguan kesehatan secara tidak langsung. Kata kunci: Muara Kali Wiso; Bakteri Coliform; Bakteri Escherichia coli ABSTRACT Kali Wiso is the river in the middle of Jepara. This river receives wastes disposal from surrounding across. The waste including domestic waste, market waste, ship waste, and waste from fish market. Based on the inputs of the waste that made the estuary polluted. Polluted waters can be seen from the observation of physical, chemical, and biological. The conditions of the waters which biologically polluted are recognized from the pathogenic bacteria existing in these waters. The indicator of bacteria used, namely coliform bacteria, because of its positive correlation with other pathogenic bacteria. The utilization of these waters is used for the activities of the port, fishing pout, and fishing activities in the waters around Jepara. Therefore, its important to know the density of coliform bacteria so that can be advantageous according to its purpose. The purpose of this study to determine total of coliform bacteria and the existence of Escherichia coli bacteria. This research conducted in March 2016 at Kali Wiso estuary with on the condition of ups and downs with two repetitions. The method used is a survey with purposive sampling technique. Laboratory analysis method used by ISO -01-2332-1991. The density of coliform bacteria in the waters of the Kali Wiso estuary is >110.000 cells/100ml and Escherichia coli bacteria is >110.000 cells/100ml. On the condition of ups and downs density of coliform bacteria and Escherichia coli have the same approximate value, but it does’nt signify that the total of bacteria both are the same. The density of coliform bacteria and Escherichia coli have exceeded the water quality criteria that have been set. The existence of these pathogenic bacteria can contaminate the biota in aquatic. Therefore, this biotics are consumed by humans, it can cause health problem indirectly. Keywords: Kali Wiso Estuary; Coliform Bacteria; Escherichia coli Bacteria


Jurnal BIOMA ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 90 ◽  
Author(s):  
Inggit Saridewi ◽  
Arief Pambudi ◽  
Yulia Fitria Ningrum

The increasing human activity makes the preference for fast food increases. However, some people do not pay attention to the hygiene conditions of food processed from food stalls. Food handlers, equipment utilization, food processing, clean water, and the packaging are the critical points of bacterial contamination. Escherichia coli is a bacterium that usually used as the indicator of food hygiene. The objective of this study is to examine the contamination of coliform bacteria, especially E. coli at two hospital cafeteria by using MPN method and questionnaire regarding the implementation of the basic principles of hygiene. Stages of tests performed that are the presumption test, confirmation test, complementary test, gram stain test, biochemical test IMViC and supported by a questionnaire. From the two locations tested, some samples showed positive result in a presumption test and confirmation test but negatively complementary to biochemical test. This indicates that the sample does not contain E. coli bacteria in food, but there is the possibility of Citrobacter. The negative results of the IMVIC test showed that it is possible bacteria found in the presumption test and confirmation test not E. coli and non-pathogenic bacteria. Based on the results of the questionnaire, most of restaurant owner has understood to served food. Food at the hospital X and Y cafetaria are safe to consume because it has a negative E.coli.


Author(s):  
Svetlana Aleksandrovna Shemetova ◽  
Tatyana Vasilievna Nikeshina ◽  
Nina Vitalievna Polyanskaya ◽  
Gennadiy Leonidovich Shendo ◽  
Rudolf Sergeevich Arakelyan ◽  
...  

The article presents the material on the sanitary-parasitological state of water bodies of the Astrakhan region for 2011–2020. In total, during the analyzed period, laboratory studies were conducted on 77,017 samples taken from environmental objects (soil, water, flushes from solid household surfaces). The share of water samples in the structure of general sanitary and parasitological studies of the environment was 7.6 % (5858 samples), including unsatisfactory samples was 3.0 % (175 samples). The structure of positive findings in water samples was represented by 6 nosological forms of helminthiasis — 88.0 % (154 samples) and 3 nosoforms of protozoa — 12.0 %. Water samples were taken from various sources in Astrakhan and the Astrakhan region and examined for the presence of eggs and larvae of helminths, cysts of pathogenic intestinal protozoa and oocysts of cryptosporidia: water from open reservoirs, centralized water supply, swimming pools, waste water, water from wells and bottled water. As a result of the conducted studies, the results were obtained, indicating that the parasitic contamination of water bodies of the Astrakhan region remains very intense, as evidenced by positive findings in the form of eggs and larvae of helminths and cysts of pathogenic intestinal protozoa; the presence in the studied water samples of eggs and larvae of helminths and pathogenic protozoa cysts, evidence of the contamination of these objects feces of infected people (eggs broad tapeworm, Ascaris, opisthorchis, pinworms, Giardia cysts, amoebas and blastocyst) and animals (egg opistorchis, Toxocara, larvae strongylid); the presence of positive findings in the sewage samples indicates poor disinfestation data objects; the presence of parasitic agents in water samples taken from open reservoirs indicates that they are contaminated with the feces of infected animals and people, or that the object is contaminated with sewage and / or sewage.


1994 ◽  
Vol 30 (8) ◽  
pp. 295-302 ◽  
Author(s):  
N. Mezrioui ◽  
B. Oudra ◽  
K. Oufdou ◽  
L. Hassani ◽  
M. Loudiki ◽  
...  

The stabilization pond is one of the more important biological wastewater treatment systems, applied in many countries. An experiment treating wastewater by stabilization ponds under the arid climate of Marrakesh (Morocco) has been underway since 1985. The experimental installation, made from two lined stabilization ponds, received domestic sewage which carried not only the organic load but also a significant bacterial load and other microorganisms. In this new habitat, the cells' bacterial behaviour was affected by various physico-chemical and biological factors. It appears that in such treatment system, known for excessive algal production, the microalgae has evidently an influence on bacterial growth. In this paper, we proposed to appreciate how microalgae essentially: Chlorella (Chlorophyta), Synechococcus andSynechocystis (Cyanobacteria), can affect the behaviour, survival and temporal evolution of Escherichia coli and Vibrio cholerae. In wastewater stabilization ponds of Marrakesh high levels of V. cholerae and low concentrations of coliform bacteria were noted during summer periods. This period coincided with a bloom of picocyanobacteria associated with a weak relative abundance of Chlorella. Some interactions tests were carried out with these bacteria and these algae, using a treated wastewater batch culture. Results show that the green algae reduces V. cholerae (pathogenic bacteria) abundances more than E. coli (fecal contamination bacteria) where as better survival of this pathogenic bacteria was noted in presence of Cyanobacteria. The die-off of E. coli appears to be more reduced in presence of Cyanobacteria than Chlorella. Furthermore, the alkaline pH seems to present a more bactericidal effect on E. coli than on V. cholerae. Thus, the Cyanobacteria blooms, associated with a weak percentage of Chlorella abundance, occurring periodically during summer in sewage stabilization ponds of Marrakesh, will be considered as one of the major factors leading to high levels of V. cholerae and low abundances of fecal coliform bacteria during the hot period.


2018 ◽  
Vol 51 (2) ◽  
pp. 134
Author(s):  
N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽  
A. ABRAHIM (Α. ΑΜΠΡΑΧΙΜ) ◽  
E. THEOLOGIDOU (Ε. ΘΕΟΛΟΓΙΔΟΥ) ◽  
Pr. KARAIOANNOGLOU (ΠΡ. ΚΑΡΑΪΩΑΝΝΟΓΛΟΥ) ◽  
A. KANSOUZIDOU (Α. ΚΑΝΣΟΥΖΙΔΟΥ)

A survey was conducted to evaluate the microbiological quality of sliced, vacuumpacked, surimi-based imitation crab at the retail level, during a 3-month storage period. The roll shaped product imported from France, was sliced and packed in a fishery processing plant in the region of Thessaloniki. Twenty five samples were examined each month, and a total of 75 samples were examined during the three month storage period of the product. Psychrotropic, lactic acid and coliform bacteria, Escherichia coli I, yeasts, Staphylococcus aureus, Salmonella spp., Listeria spp. and Escherichia coli Θ157:Η7, were used as microbial indices, to evaluate the microbiological quality of the product. The following conclusions were derived from the evaluation of the results of the microbiological examinations. 1) The microbiological condition of the crab meat analogs examined on the 15th day of storage, was found satisfactory, since microbial populations were very low, while pathogenic bacteria were not detected in any of the examined samples. 2) The overall microbiological quality of the samples examined at the end of the second month of storage, was also good, since psychrotrophic and lactic acid bacteria exceeded 1Θ7 log cfu/g only in one sample), while coliform bacteria were not detected in 60% of the samples and Escherichia coli I was detected only in one sample. The mean value of the yeasts was 2.84 log cfu/g, while molds were not detected in any of samples examined. 3) The microbiological quality of the samples examined at the end of the three months storage period was not satisfactory, since the microbial indices in the majority of the samples (80%), were rather high. S. aureus, Salmonella spp, Listeria s pp. and E. coli 0157:H7 were not detected in any sample, during the three month storage period.


2021 ◽  
Vol 23 (1) ◽  
pp. 163-168
Author(s):  
Rudolf S. Arakelyuan ◽  
Khalil M. Galimzyanov ◽  
Anna E. Maslyaninova ◽  
Tatyuna M. Deeva

The sanitary and parasitological state of environmental objects of the Astrakhan region is analyzed. It was found that out of 689 samples taken and studied from various environmental objects, 114 (16,5%) did not meet sanitary and parasitological standards. The share of soil samples taken was 554 (80,4%), of which 107 (19,3%) did not meet sanitary and parasitological standards. In 83 (15%) cases, living larvae of Strongyloides stercoralis were found, in 12 (2,1%) fertilized eggs of Ascaris lumbricoides, in 10 (1,8%) eggs of Toxocara canis, in 1 (0,2%) sample mixed invasions were noted: fertilized eggs of Ascara canis of 126 (18,3%) washes from hard surfaces, 4 (8,6%) were unsatisfactory (in 7,35% of cases, fertilized eggs of Ascaris lumbricoides were found and in 1,25% eggs of Enterobius vermicularis. Dead larvae of Anisakis simplex were found in 3 (0,4%) fish samples examined. All 6 (0,9%) river water samples complied with sanitary and parasitological standards. The presence of living strongilid larvae and toxocar eggs in the soil indicates its contamination with faeces of infected animals. The presence of people or about flooding or flooding of these objects sewer drains allows to assume existence of oosperms of ascarids in the soil about her pollution by excrements. The presence of eggs of ascarids and pinworms on various household surfaces indicates non-compliance with the rules of personal hygiene of persons infected with ascarids and/or pinworms who use these household appliances. The presence of aniacid larvae in the internal organs of fish is not a contraindication for its sale to the population.


2011 ◽  
Vol 74 (9) ◽  
pp. 1543-1546 ◽  
Author(s):  
SHIGENOBU KOSEKI ◽  
YASUKO MIZUNO ◽  
SUSUMU KAWASAKI ◽  
KAZUTAKA YAMAMOTO

No information has been available on the prevalence of pathogens in fresh produce in Japan. In the present study, information was collected on the occurrence of contamination by Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes in iceberg lettuce in a Japanese retail store. A total of 419 samples of lettuce that had been harvested in different districts and/or by different producers from July 2008 to March 2009 were examined. A multiplex PCR method was used to simultaneously identify the three bacterial pathogens. No pathogenic bacteria, including Salmonella, E. coli O157:H7, and L. monocytogenes, were detected from any of the samples with this highly sensitive and validated procedure. The aerobic bacteria plate counts and coliform bacteria counts in lettuce throughout the examination period did not show any seasonal trends, and the numbers were comparable to those reported by others from around the world. Based on the results of this study, we concluded that none of the three major pathogens were present in this limited survey of iceberg lettuce sold by a retailer in Japan.


2021 ◽  
Vol 38 (2) ◽  
pp. 126-132
Author(s):  
Viktoria A. Irdeeva ◽  
Svetlana A. Shemetova ◽  
Rudolf S. Arakelyuan ◽  
Gennadyi L. Shendo ◽  
Nina V. Polyanskaya

Objective. We studied the contamination of the soil with helminth-protozoan infestations in Astrakhan region for 20102019. Materials and methods. The research work was carried out in the Laboratory of Bacteriological and Parasitological Research of the Center for Hygiene and Epidemiology in the Astrakhan Region in 20102019. A total of 10 692 soil samples were examined, and 11 384 studies were performed. The number of samples, which did not meet the sanitary and parasitological indicators was 7.0 % (753 samples). Eggs and larvae of helminths, as well as cysts of pathogenic intestinal protozoa were found in these samples. Results. During the analyzed period, 86 491 samples of sanitary and parasitological studies were selected and examined in the Astrakhan region, of which 93.6 % (80 962 samples) were samples taken from various environmental objects (water, soil, manure, flushes from solid household surfaces). The total share of soil samples in the structure of all studies of environmental objects was 13.2 % (10 692 samples). Conclusions. Parasitic contamination of the soil remains tense, as evidenced by positive findings in the form of eggs and larvae of helminths, as well as cysts of pathogenic intestinal protozoa. The presence of toxocara eggs and strongylid larvae in the soil indicates contamination of this object with the feces of infected animals. The presence of eggs of ascarids, opisthorchis, pygmy tapeworm and cysts of dysentery amoeba in the soil may indicate that this object is contaminated with the feces of persons with parasitic infestation, or about accidents on the sewer network and underflooding of soil sampling sites.


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