scholarly journals Remineralizing Effect of Xilitol, Juniperus Communis and Camellia Sinensis Added to a Toothpaste: An In Vitro Study

2018 ◽  
pp. 97-105
Author(s):  
Sandra I. Jiménez-Gayosso DDS ◽  
Edith Lara-Carrillo DDS, MSc, PhD ◽  
Rogelio J. Scougall-Vilchis DDS, MSc, PhD ◽  
Raúl A. Morales-Luckie BS Chem, PhD ◽  
Carlo E. Medina Solís DDS, MSc ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Pearson Correlation ◽  
In Vitro Study ◽  
Control Group ◽  
Sem Images ◽  
Juniperus Communis ◽  
Pearson Correlation Test ◽  
Pre Treatment ◽  
The One

The aim of this study was to explore the remineralizing effect of toothpastes based on Xilitol, Camellia Sinensis and Juniperus Communis. An in vitro experimental study was carried out in an 18 human premolars sample, which were treated with one of the 3 evaluated toothpastes and a control fluoride one. The atomic percentages of Ca and P were evaluated by Energy Dispersive X-ray Spectroscopy (EDS). In addition, the enamel surface of treated teeth was visualized by Scanning Electron Microscopy (SEM). The evaluations were carried out in three stages: pre-treatment, after an artificial demineralization process and after the treatment with the toothpastes. In the statistical analysis, the one-way ANOVA and Pearson correlation tests were used. Xilitol and Juniperus Communis showed the greatest gain of P ions compared to the control group (p<0.01). As for Ca, the group treated with xylitol-based toothpaste showed more ion gain compared with the control group (p<0.01). In the Pearson correlation test between Ca and P, statistically significant correlations were observed in all groups (p<0.01), ranging between r=0.7413 (Xylitol Group) and r=0.9510 (Control Group). We concluded that Xylitol paste showed the highest remineralizing property, both in the EDS analysis and in the SEM images.

Comparative evaluation of shear bond strength of composite to dentin in presence of different dental liners: An in-vitro study

2021 ◽  
Author(s):  
Ladan Ranjbar Omrani ◽  
Saba Tohidkhah ◽  
Elham Ahmadi ◽  
Mahdi Abbasi ◽  
Reza Morvaridi Farimani
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Control Group ◽  
The One ◽  
Post Hoc

Abstract Background: The aim of the current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

scholarly journals Is it necessary to pre-treat Dentine before GIC Restorations? Evidence from an in Vitro Study.

2020 ◽  
Vol 33 (1) ◽  
pp. 27-32
Author(s):  
Julio Bassi ◽  
Tamara Tedesco ◽  
Daniela Raggio ◽  
Ana Maria Santos ◽  
Renata Bianchi ◽  
...  
Keyword(s):  
Phosphoric Acid ◽  
Polyacrylic Acid ◽  
Treatment Approach ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Acid Group ◽  
Control Group ◽  
Bonding Performance ◽  
Pre Treatment

The aim of this study was to assess the influence of different pre-treatment approaches on glass ionomer cement (GIC) bond strength (BS) to dentine. Sixty bovine incisors were allocated into six groups according to substrate - sound or caries-affected dentine; and substrate pre-treatment approach - no conditioning (control), polyacrylic acid for 10 s and phosphoric acid for 7 s. Teeth in the caries-affected dentine group were previously submitted to cariogenic pH-cycling challenge. After dentine pre-treatment, according to experimental groups, polyethylene tubes were placed on flat dentine surfaces and filled with GIC. Teeth were stored in distilled water for 24 h at 37 °C and then submitted to microshear test (0.5 mm/min). Failure pattern analysis was performed under stereomicroscope (400x). Data were analysed using two-way ANOVA and Tukey's test (α=5%). Statistically significant differences were found for the pre-treatment approach, regardless of substrate (p<0.001). The polyacrylic acid group and control group had similar BS values, and were both better than the phosphoric acid group. In general, GIC had better bonding performance in sound dentine than in caries-affected dentine. In conclusion, dentine pre-treatment with polyacrylic acid did not improve the performance of GIC restoration on clinically relevant substrates.

Comparative Evaluation of Shear Bond Strength of Composite to Dentin in Presence of Calcium Releasing Liners: An In-Vitro Study

2021 ◽  
Author(s):  
Saba Tohidkhah ◽  
Elham Ahmadi ◽  
Mahdi Abbasi ◽  
Reza Morvaridi Farimani ◽  
Ladan Ranjbar Omrani
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Control Group ◽  
The One ◽  
Post Hoc

Abstract Background: The aim of current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

scholarly journals Effect of high-power diode laser on radical surfaces exposed as dental tubules obliteration: in vitro study

2021 ◽  
Vol 10 (9) ◽  
pp. e42410918049
Author(s):  
Roberta Janaína Soares Mendes ◽  
Guilherme Silva Furtado ◽  
Nayanna Matos Sousa ◽  
Daniele Meira Conde Marques ◽  
Rafael Soares Diniz ◽  
...  
Keyword(s):  
Diode Laser ◽  
High Power ◽  
In Vitro Study ◽  
Control Group ◽  
Sem Images ◽  
Human Teeth ◽  
Dentin Tubules ◽  
Power Diode ◽  
High Power Diode Laser

The aim of this study was to find adequate parameters of high-power diode laser for obliteration of dental tubules. Newly extracted human teeth (molars) were used for the research that were treated with high-power diode laser and then evaluated by scanning electron microscopy. Roots of 10 healthy teeth were used and prepared in 40 dentin blocks and dividing them into 4 groups: Control Group; G1, G2, G3 (groups treated with high-power diode laser), varying power, energy and application time. The images were evaluated randomly by 2 blinded, calibrated examiners who attributed scores to each image with a level of significance of 5%. The null hypothesis there was no difference between the groups tested regarding of the obliteration of the dentinal tubules. Considering the scores used for analyzing the SEM images, statistically significant differences were observed between the control and all the experimental groups (p < 0,05). However, the irradiated (experimental) groups showed no statistically significant differences between them, because at all the tested parameters the dentin tubules were shown to be obliterated (p < 0.05). The efficacy can be completed in the obliteration of tubules and interruption of fluid movement within the dentin tubules with treatment of the exposed surface with the high-power diode laser; however, the parameters of power and energy lowest showed better results.

Comparative evaluation of shear bond strength of composite to dentin in presence of different dantal liners: An in-vitro study

2021 ◽  
Author(s):  
Ladan Ranjbar Omrani ◽  
Saba Tohidkhah ◽  
Elham Ahmadi ◽  
Mahdi Abbasi ◽  
Reza Morvaridi Farimani
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Control Group ◽  
The One ◽  
Post Hoc

Abstract Background: The aim of the current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

An In Vitro Evaluation of the Effect of Dimethyl Sulfoxide Pre-Treatment on the Bond Strength of Fiber Post

2021 ◽  
Vol 13 (4) ◽  
pp. 258-262
Author(s):  
Reshma Rajasekhar ◽  
Sooraj S ◽  
Midhula Sathyan ◽  
Varsha Maria Sebastian
Keyword(s):  
Bond Strength ◽  
Dimethyl Sulfoxide ◽  
In Vitro Study ◽  
Treated Group ◽  
Control Group ◽  
Significant Difference ◽  
Pre Treatment ◽  
Group B ◽  
Post Space

Background: The purpose of this in vitro study was to evaluate the efficacy of dimethyl sulfoxide pre-treatment of dentin on the bond strength of fiber post. Methods: Twenty single rooted mandibular premolar teeth were decoronated and shaping and cleaning was done till Protaper gold F3. After obturation, the teeth were sealed using type II GIC and stored in the distilled water for one week. Post space preparation was done using peeso reamer to a depth of 12 mm with 5 mm of remaining gutta percha in the apical portion of all samples and post space was etched with 37% phosphoric acid. The specimens were divided into two groups with 10 teeth each in group A (DMSO group) and group B (Control). In the group A, the post space was treated with 1 mL of 50% v/v dimethyl sulfoxide (DMSO) and in group B (control), the post space was treated with 1 mL of distilled water for 60 seconds with the help of paper points. Etch and rinse resin cement (Variolink II, IvoclarVivadent) was used following the manufacturer’s instructions. Fibre posts were coated with cement and placed into the post space and light cured. Push out strength of samples was performed using universal testing machine. Results: Statistical analysis of the data showed no significant difference in push out bond strength between the control group and pre-treated group. Conclusion: This in vitro study showed that there was no significant difference in the immediate bond strength scores of 50% v/v dimethyl sulfoxide pre-treated group when compared to control group. Keywords: Dentin, Dimethyl sulfoxide, Dowel, Resin cements

scholarly journals Effect of Three Different Remineralizing Agents on Enamel Caries Formation – An in vitro Study

2015 ◽  
Vol 12 (1) ◽  
pp. 16-20 ◽  
Author(s):  
N Sathe ◽  
RVS Raju Chakradhar ◽  
V Chandrasekhar
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Calcium Content ◽  
Control Group ◽  
Sem Images ◽  
Group Iii ◽  
Enamel Caries ◽  
Group I ◽  
Tooth Mousse

Background Caries process is not a static one, but is dynamic with interspersed periods of demineralization and remineralization of enamel, intimately related and occurs episodically based upon the presence of cariogenic bacteria in dental plaque and the availability of refined carbohydrates for fermentation to organic acids.Objectives Early enamel caries could be reversed with avoidance of frank cavitation. The main objective of this study is to check whether enamel demineralization can be prevented by using the remineralizing agents.Method Forty freshly extracted human central incisors were selected and stored in saline at normal temperature. A window of 3 X 3mm enamel was created and all the specimens were then randomly divided into 4 groups of 10 each. Group I- teeth received no treatment, Group II- teeth treated with Acidulated Phosphate Flouride gel, Group III- teeth treated with Tooth Mousse Plus, Group IV- teeth treated with Remin+. Samples in all the groups were kept in artificial saliva for 24 hours and subjected to modified Ten Cate’s solution at an acidic pH of 3.5 for 10 days. The samples were sectioned and subjected to SEM evaluation.Result Scanning Electron Microscope(SEM) images showed decrease in pore volume of the enamel in all the treatment groups compared to the control group indicating increase in resistance to demineralization in acidic pH.Conclusion The three groups of remineralizing agents, Acidulated Phosphate Fluoride gel, Tooth Mousse Plus and Remin + showed significant increase in fluoride content and negligible increase in calcium content indicating there is remineraliztion.Kathmandu University Medical Journal Vol.12(1) 2014: 16-20

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Reverse torque values of abutment screws after dynamic loading: The effects of sealant agents and the taper of conical connections

2020 ◽  
Author(s):  
Arda Ozdiler ◽  
suleyman dayan ◽  
Burc Gencel ◽  
Gulbahar Isık-Ozkol
Keyword(s):  
Dynamic Loading ◽  
Antibacterial Agent ◽  
In Vitro Study ◽  
Control Group ◽  
Silicone Sealant ◽  
Reverse Torque ◽  
The Stability ◽  
Torque Values ◽  
Chlorhexidine Gel

This in vitro study evaluated the influence of taper angles on the internal conical connections of implant systems and of the application of chlorhexidine gel as an antibacterial agent or a polyvinyl siloxane (PVS) sealant on the reverse torque values of abutment screws after dynamic loading. The current study tested four implant systems with different taper angles (5.4°, 12°, 45°, and 60°). Specimens were divided into three groups: control (neither chlorhexidine gel filled nor silicone sealed), 2% chlorhexidine gel-filled or silicone-sealed group, and group subjected to a dynamic load of 50 N at 1 Hz for 500,000 cycles prior to reverse torque measurements. Quantitative positive correlation was observed between the taper angle degree and the percentage of tightening torque loss. However, this correlation was significant only for the 60° connection groups except in the group in which a sealant was applied ( p = 0.013 for the control group, p = 0.007 for the chlorhexidine group). Percentages of decrease in the torque values of the specimens with silicone sealant application were significantly higher compared with both the control and chlorhexidine groups ( p = 0.001, p = 0.002, p = 0.001, and p = 0.002, respectively, according to the increasing taper angles); the percentage of decrease in torque values due to chlorhexidine application was statistically insignificant when compared with the control group. The application of gel-form chlorhexidine as an antibacterial agent does not significantly affect the stability of the implant–abutment connection under dynamic loads. PVS sealants may cause screw loosening under functional loads.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

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