Effect of voluntary exercise on intracellular signalling pathways of angiogenesis in the sciatic nerve of type 1 diabetic castrated male rats

2019 ◽  
Vol 106 (1) ◽  
pp. 39-47 ◽  
Author(s):  
SB Zanjani ◽  
L Chodari ◽  
FM Bavil ◽  
P Sadeghzadeh ◽  
P Shahabi
Keyword(s):  
Sciatic Nerve ◽  
Network Formation ◽  
Diabetic Rats ◽  
Voluntary Exercise ◽  
Male Rats ◽  
Histological Evaluation ◽  
Diabetes Group ◽  
Glucose Levels ◽  
Phosphorylated Akt

Objectives Impaired angiogenesis in sciatic nerve is a major complication of diabetic neuropathy. Protein kinase B (AKT) and extracellular signal regulated kinase (ERK) signaling pathways play critical roles during capillary-like network formation in the angiogenesis process. Methods Twenty-four adult male Wistar rats (weight: 250–300 g) were used in the research. The rats were randomly divided into four groups (n = 6): (1) diabetic (Dia), (2) diabetic + castration (Dia-Cas), (3) diabetic + exercise (Dia-E), and (4) diabetic + castration + exercise (Dia-Cas-E). Type 1 diabetes (T1D) was induced with streptozotocin (50 mg/kg). After 6 weeks, sciatic nerve was separated and used for histological evaluation and determination of phosphorylated AKT (P-AKT) and phosphorylated ERK (P-ERK) levels by ELISA method. Results Glucose levels decreased in the Dia-E group compared to the Dia-Cas group (p < 0.01). In addition, our finding shows that exercise in the Dia-Cas group diminished blood glucose levels compared to the Dia-Cas group but this effect of exercise was not significant. Voluntary exercise in the diabetic castrated group decreased P-AKT protein and increased P-ERK 1/2 protein levels in the sciatic tissue compared to the diabetes group significantly (p < 0.05). Histopathological findings showed that Dia-Cas group with 6-week exercise training significantly raised the number of microvascular density in the sciatic tissue of diabetic rats compared to the diabetic group (p < 0.05). Conclusions Voluntary exercise in diabetic rats increases angiogenesis in the sciatic nerve. The possible mechanism is the increase of P-ERK 1/2 but not P-AKT levels in the sciatic nerve of T1D rats.

scholarly journals The effect of Diplotaenia turcica root extract in streptozotocin-induced diabetic rats

2020 ◽  
Vol 45 (2) ◽  
Author(s):  
Uğur Özdek ◽  
Serkan Yıldırım ◽  
Yeter Değer
Keyword(s):  
Antioxidant Property ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
Root Extract ◽  
Diabetes Group ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Pancreatic Damage

AbstractBackgroundDiplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated.Materials and methodsIn this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests.ResultsIt was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT.ConclusionThese results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.

The ability of active fraction from Kappaphycus alvarezii (Doty) Doty Ex.P.C.Silva to Decrease Glucose Levels inhibit advanced Glycation End-Products (AGEs) Formation and RAGE (AGER) Gene Expression in Diabetic Rats

2020 ◽  
Author(s):  
Mae Sri Hartati Wahyuning ◽  
Evy - Yulianti ◽  
Sunarti - Sunarti
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Kappaphycus Alvarezii ◽  
Glycated Albumin ◽  
Diabetic Rats ◽  
Male Rats ◽  
Diabetic Rat ◽  
Active Fraction ◽  
Glucose Levels ◽  
Significant Difference

Abstract Background. Kappaphycus alvarezii (Doty) Doty ex P.C.Silva is a widely used seaweed that has antioxidant and antiglycation activities. The purpose of this study was to examine the ability of active fraction from Kappaphycus alvarezii to decrease glucose level and inhibit glycation process. Methods. This study used bioassay-guided fractionation through three stages of the extraction, partition, and fractionation processes that were monitored using Thin Layer Chromatography and BSA-Glucose test. Inhibition of glycation was known by calculating percentage of inhibition and IC50. Selected active fraction was used for in vivo tests using 24 Wistar male rats. Measurement of glucose levels used GOD-PAP method, while levels of glycated albumin (GA) and Nε- (carboxymethyl) lysine (CML) were measured using ELISA. Analysis of RAGE gene expression used qPCR. Results Glycation test showed a significant difference (p < 0.05) between all treatments. Chloroform extract showed higher percentage of inhibition (62.4 ± 3.45%) with lower IC50 (0.33 ± 0.01 mg/ml) compared to methanol extract (0.52 ± 0.03 mg/ml). Methanol-soluble extracts had a higher percentage of inhibition (51.10 ± 1.64%) with IC50 0.45 ± 0.05 mg/ml compared to methanol-insoluble extract (1.25 ± 0.05 mg/ml). Fraction II had a higher percentage of inhibition (53.37 ± 1.92%) with IC50 0.12 ± 0.01 mg/ml compared to other fractions. Selected active fraction reduced blood glucose by 1.3% and 5.2% and CML levels by 50.6% and 42.4% at concentrations of 0.17 and 0.255 mg/ml in diabetic rats. RAGE gene expression was lower in the diabetic rat groups treated with active fraction compared to untreated diabetic group. Conclusions The active fraction has ability for reducing blood glucose, antiglycation, or reducing CML levels, and RAGE gene expression.

scholarly journals Can troxerutin pretreatment prevent testicular complications in prepubertal diabetic male rats?

2020 ◽  
Vol 54 (2) ◽  
pp. 85-95
Author(s):  
Afsaneh Ghadiri ◽  
Fariba Mirzaei Bavil ◽  
Gholam Reza Hamidian ◽  
Hajar Oghbaei ◽  
Zohreh Zavvari Oskuye ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Blood Glucose ◽  
Serum Insulin ◽  
Insulin Level ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Male Wistar Rats ◽  
Apoptosis Process

AbstractObjective. The vast majority of type 1 diabetes leads to a higher prevalence of reproductive system’s impairments. Troxerutin has attracted much attention owing to its favorable properties, including antihyperglycemic, anti-inflammatory, and antiapoptotic effects. This investigation was proposed to evaluate whether pretreatment with troxerutin could prevent apoptosis-induced testicular disorders in prepubertal diabetic rats.Methods. Fifty prepubertal male Wistar rats were randomly allocated into five groups: control (C), troxerutin (TX), diabetic (D), diabetic+troxerutin (DTX), and diabetic+insulin (DI). Diabetes was induced by 55 mg/kg of streptozotocin applied intraperitoneally. In TX and DTX groups, 150 mg/kg troxerutin was administered by oral gavage. Diabetic rats in DI group received 2–4 U NPH insulin subcutaneously. Troxerutin and insulin treatments were begun immediately on the day of diabetes confirmation. After 30 days, the testicular lipid peroxidation and antioxidant activity, apoptosis process, and stereology as well as serum glucose and insulin levels were assessed.Results. The results showed that diabetes caused a significant increase in the blood glucose, the number of TUNEL positive cells and tubules, and the malondialdehyde level as well as a significant decrease in serum insulin level compared to controls. The stereological analysis also revealed various alterations in diabetic rats compared to controls. Troxerutin treatment improved these alterations compared to the diabetic group.Conclusion. Troxerutin-pretreatment may play an essential role in the management of the type-1 diabetes-induced testicular disorders by decreasing blood glucose and modulating apoptosis.

scholarly journals Evaluation of ameliorative effect of sodium nitrate in experimental model of streptozotocin-induced diabetic neuropathy in male rats

2019 ◽  
Vol 53 (1) ◽  
pp. 14-25 ◽  
Author(s):  
Hajar Oghbaei ◽  
Mohammad Reza Alipour ◽  
Gisou Mohaddes ◽  
Gholam Reza Hamidian ◽  
Rana Keyhanmanesh
Keyword(s):  
Serum Insulin ◽  
The Body ◽  
Male Rats ◽  
Diabetes Group ◽  
Insulin Levels ◽  
Nitrate Treatment ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ameliorative Effect ◽  
Male Wistar Rats

AbstractObjective. Diabetes induces sensory symptoms of neuropathy as positive (hyperalgesia), negative (hypoalgesia), or both.Methods. In the present study, fifty male Wistar rats were allocated to five groups: control, control+nitrate, diabetes, diabetes+insulin, and diabetes+nitrate. Thirty days after diabetes confirmation, insulin (2–4 U/day) was injected subcutaneously in diabetes+insulin group and nitrate (100 mg/l) was added into drinking water of the control+nitrate and diabetes+nitrate groups for a period of 2 months. In order to assess the mechanical and thermal algesia, tail immersion, hot plate, and von Frey tests were performed. The serum insulin levels were determined with insulin ELISA Kit. Serum level of NOx was determined by the Griess method.Results. Both thermal and mechanical nociceptive thresholds showed a significant decrease (p<0.05) which was followed by a significant increase (p<0.01) in the thermal nociceptive threshold in the diabetes group. Chronic nitrate or insulin treatment led to a significant decrease (p<0.01) in blood glucose levels, as well as a significant (p<0.05) increase in the body weight and serum NOx. Moreover, nitrate treatment significantly increased serum insulin levels (p<0.001) compared to the other groups.Conclusion. Chronic nitrate treatment modified the thermal and mechanical sensitivities in diabetic animals.

Dioscorea dumetorum-Fed Rats Exhibited Decreased Body Weight, Blood Glucose, and Insulin in STZ-induced Diabetes

2014 ◽  
Vol 4 (2) ◽  
pp. 87 ◽  
Author(s):  
Henrietta Aritetsoma Ogbunugafor ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Chinwe Nonyelum Ezekwesili ◽  
Chike Samuel Okafor ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Feed Intake ◽  
Weight Reduction ◽  
Blood Glucose Concentration ◽  
Insulin Level ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Preventive measures that could slow down the rising incidences of diabetes mellitus are essential. The use of neglected local foods, which have effects on this chronic disease beyond basic nutrition as dietary controls, is desirable.Objective: The effect of Dioscorea dumetorum (Kunth) Pax (Dioscoreaceae) feed on satiety, weight, blood glucose, and insulin levels were investigated in streptozotocin-induced diabetic rats.Methods: Twenty adult male rats in four groups of five were used for the experiment. Three groups – D. dumetorum, glibenclamide, and standard pellet-fed rats were induced with diabetes by i.p. administration of 50mg kg-1 streptozotocin, while the fouth group (?) served as a non-diabetic control. D. dumetorum was fed at 15g daily for ten days before induction, and after induction, feeding continued. Glibenclamide was orally administered 5mg kg-1 daily. Both the untreated and non-diabetic rats were kept on standard rat pellets. Feed intake, weight, and blood glucose concentration were monitored daily, while insulin level was measured on day two and day six after inductions. Results: Average feed intake for non-diabetic rats was 15g for D. dumetorum per day, which dropped to 10.3g after induction of diabetes. Weight of normal non-diabetic rats consistently increased (142.61 ± 4.37g – 169.43 ± 8.61g) for the duration (17 days) of the experiment. The D. dumetorum-fed rats showed weight reduction of 5.4%, glibenclamide 4.0%, and untreated diabetic 6.15%. Non-diabetic rats blood glucose levels ranged between 70 to 100mg dL-1. Streptozotocin (STZ) (i.p.) administration increased blood glucose levels from 370% to 626% in the rats. D. dumetorum-fed rats showed reduced (p<0.05) blood glucose levels of 22.6%. Glibenclamide had 5.5% reduction (p<0.05). Insulin was absent in D. dumetorum-fed rats, whereas 0.95ng ml-1 of insulin was detected in glibenclamide-administered rats. These quantities were lower (p<0.001) than 1.40ng ml-1 in the non-diabetic rats. Conclusion: This study revealed that D. dumetorum tuber caused decreased hunger, weight reduction, and displayed hypoglycemic property in diabetic rats, even after heat treatment. Its probable mechanism of anti-hyperglycemic activity might not be through increased insulin secretion.Key words: D. dumetorum, streptozotocin-induced diabetes, weight, blood glucose, insulin.

Gender-Specific differences in pulsed magnetic field exposed to diabetic neuropathic rats

2019 ◽  
Author(s):  
Iþýl Öcal ◽  
M. Bertan Yýlmaz ◽  
Aykut Pelit ◽  
Fatma Çoban ◽  
Bora Taºtekin ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Weight Loss ◽  
Type 1 Diabetes ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Pulsed Magnetic Field ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Mechanical Threshold

Type-1 diabetes mellitus is an insulin-dependent autoimmune disease, which is very common in the human populations regardless of gender. The aim of this study was to evaluate the effects of pulsed magnetic field (PMF), a non-invasive procedure, on male and female rats with type 1 diabetes, particularly on weight loss ratios, blood glucose levels and diabetic neuropathy. Before, the experimental groups were divided into three groups as control (C (F or M), diabetes (DM (F or M), controlled diabetes (DM(F)-INS or DM(M)-INS) groups according to their sex differences, then these experimental groups were exposed to magnetic field effect (PMF). The rats in the PMF groups were exposed to the pulsed magnetic field at 50 Hz (1.5 mT intensity) for 1h/5days/month. The body weights and blood glucose levels were measured once a week over a month. Female and male diabetic rats developing diabetic neuropathy were evaluated with thermal (thermal latency) and dynamic (mechanical threshold) plantar tests. After six-weeks of PMF treatment, the weight loss rate and increased blood glucose levels due to diabetes reversed in both female and male diabetic rats upon PMF treatment (p less than 0.05). In diabetic neuropathic female and male rats, the thermal latency values increased, while the mechanical threshold values decreased. The reduction in diabetic neuropathic rats were statistically significant in diabetic rats (p less than 0.05). The increased or decreased mechanical threshold and thermal latency values in diabetic neuropathic rats were statistically significant in only male diabetic rats (p less than 0.05). Our studies may imply that the effect of PMF in neuropathic pain is gender dependent further inferring that hormonal mechanisms were also important in PMF dependent regulation.

Possible role of blood insulin levels on glutathione S-transferase activities from different tissues of male rats

1990 ◽  
Vol 68 (2) ◽  
pp. 170-173 ◽  
Author(s):  
Cristina E. Carnovale ◽  
Juan A. Monti ◽  
Viviana A. Catania ◽  
Maria C. Carrillo
Keyword(s):  
Diabetic Rats ◽  
Male Rats ◽  
Blood Levels ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Insulin Levels ◽  
Blood Insulin ◽  
Glucose Levels

The activity of in vitro glutathione S-transferase towards 1-chloro-2,4-dinitrobenzene was examined in liver, renal cortex, and small intestine (duodenum, jejunum, ileum) after the in vivo treatment of male Wistar rats with streptozotocin or alloxan. The studies were performed at 2, 10, 24, and 48 h and 7 and 15 days after streptozotocin treatment or 24 and 48 h after alloxan treatment. The results indicated that while the blood levels of insulin–glucose did not show variations, there were no alterations of the glutathione S-transferase activity in the tissues tested. On the other hand, when the treatments caused modifications on blood insulin–glucose levels, there were changes of glutathione S-transferase activity in all tissues (except in the ileum) in such a way that a direct relationship between plasma insulin levels and glutathione S-transferase activity could be demonstrated. These results were also confirmed through insulin administration to control and diabetic rats. The data demonstrate a possible regulation of glutathione S-transferase activity by blood insulin and (or) glucose levels in the tissues tested.Key words: insulin, glutathione S-transferase, streptozotocin, alloxan.

scholarly journals EFFECT OF BLACK RICE BRAN EXTRACT (Black Rice Bran) TO DECREASE DECREASE OF GLUCOSE LEVEL IN DIABETIC RATS

2018 ◽  
Vol 14 (1) ◽  
pp. 8-13
Author(s):  
Novita Sari ◽  
Arifah Sri Wahyuni
Keyword(s):  
Rice Bran ◽  
Negative Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Sprague Dawley ◽  
Black Rice ◽  
Rice Bran Extract ◽  
Glucose Levels ◽  
Black Rice Bran ◽  
Decrease Decrease

Black rice bran is one of processed foods derived from plants and has a decreasing effect of blood glucoselevels. Major component of anthocyanin in black rice bran that functions in lowering blood glucoseconcentration is cyanidin 3-glucoside. Fifteen male rats Sprague-Dawley strain were selected randomly anddivided into 5 groups with the same number in each groups. These groups were consisted of normal control,negative control, and black rice bran extract treatment with dose of 50, 100 and 200 mg/kg of bodyweight(b.w.). Each of them were induced to experience diabetes by alloxan 150 mg/kg of b.w. Measurement ofblood glucose levels was done by using visible spectrophotometer at 546 nm. The samples were collectedbefore and after alloxan injection, and after 4, 7, and 10 days later to observe the changes of blood glucoselevels. The results showed that black rice bran at 200 mg/kg of b.w. was able to lower the glucose levels,which amounted for 131,33 ± 8,08 mg/dL after 10 days of injection.

scholarly journals The Aerobic Training and Berberine Chloride Intervention on Pancreatic Tissue Antioxidant Enzymes and Lipid Peroxidation in Type 1 Diabetic Rats

2020 ◽  
Author(s):  
Javad Ramezani ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Aerobic Training ◽  
Diabetic Control ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
P Value ◽  
Berberine Chloride

Objective: Aerobic training and berberine chloride include antioxidant characteristics. In this study, aerobic training and berberine chloride intervention on the activity of antioxidant enzymes and lipid peroxidation of pancreatic tissue were investigated in type 1 diabetic male wistar rats. Materials and Methods: In the current study, 56 Wistar male rats were randomly assigned to seven different groups (n= 8), these groups include healthy control, diabetic control, Berberine-treated diabetes (15&30 mg/kg), aerobic training-treated diabetes, Diabetes treated with Berberine and aerobic training (15&30 mg/kg). The aerobic training schedule consisted of 6 weeks treadmills with a frequency of 5 sessions per week. The Berberine was also fed a specific dose every day and a half before training. The superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde were assessed using ELISA method. Results: The results showed that aerobic training, as well as the intervention of Aerobic Training and Berberine chloride, had a significant effect on the increase of antioxidant enzymes SOD and CAT in the pancreatic tissue groups (P-value< 0.05), but did not significantly affect the GPX level (P-value> 0.05). There was a significant decrease in MDA level in all treatment groups (P-value< 0.05). In diabetic groups that received both treatments at the same time, the MDA level more decreased (P-value< 0.0005). Conclusion: The aerobic training and berberine chloride concurrent intervention have a greater effect on the antioxidant enzymes in the pancreatic tissue of diabetic specimens. Therefore, it is recommended that aerobic training be done with berberine chloride.

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