Studies on the drought and heat stress response of green bean ( Phaseolus vulgaris L.) varieties under phytotronic conditions

2008 ◽  
Vol 56 (3) ◽  
pp. 321-328 ◽  
Author(s):  
E. Nemeskéri ◽  
J. Remenyik ◽  
M. Fári
Keyword(s):  
Heat Stress ◽  
Control Level ◽  
Water Soluble ◽  
Green Beans ◽  
Water Supplies ◽  
Heat Stress Response ◽  
Antioxidant Content ◽  
Short Period ◽  
Drought And Heat Stress ◽  
Optimum Water

The drought tolerance of six green-and yellow-podded varieties of green beans with different genetic backgrounds was tested in the phytotron. During the week prior to flowering the plants were kept either at 25/15°C (day/night) or at high temperature (30/15°C), with RH 75% and optimum water supplies. The heat-stressed plants were then divided into three groups; the first was returned to the control (25/15°C) chamber (RH 75%, optimum water supplies), while the second and third were exposed to mild drought stress (RH 60%, 50% water) at temperatures of 30/15°C and 35/25°C, respectively, throughout the flowering period.The varieties survived the short period of heat stress (30/15°C) prior to flowering without damage provided the temperature during flowering was reduced to 25/15°C and the water supplies were optimum. There was a sharp increase in the carotene level in the leaves of drought-stressed plants when the temperature during flowering was 30/15°C, but in plants exposed to 35/25°C during flowering the level dropped to near the control level. The latter group exhibited considerable damage, with a reduction in the water-soluble antioxidant content (ACW: antioxidant capacity of water-soluble substances) and the chlorophyll b content compared with the control.The antioxidant content (ACW) in the dark green leaves of green-podded varieties was lower than in the yellow-podded varieties and did not change as the result of drought and heat stress. In yellow-podded varieties, however, there was a significant decline in ACW in response to stress. Differences between the varieties in their adaptability to drought and heat could be detected as changes in the chlorophyll and carotene contents of the leaves even at 30/15°C.

scholarly journals BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis

2017 ◽  
Vol 114 (40) ◽  
pp. E8528-E8536 ◽  
Author(s):  
Kyoko Morimoto ◽  
Naohiko Ohama ◽  
Satoshi Kidokoro ◽  
Junya Mizoi ◽  
Fuminori Takahashi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Target Genes ◽  
Active Form ◽  
E3 Ligase ◽  
Protein Stabilization ◽  
Regulatory Domain ◽  
Heat Stress Response ◽  
Element Binding Protein ◽  
Drought And Heat Stress

DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A (DREB2A) acts as a key transcription factor in both drought and heat stress tolerance in Arabidopsis and induces the expression of many drought- and heat stress-inducible genes. Although DREB2A expression itself is induced by stress, the posttranslational regulation of DREB2A, including protein stabilization, is required for its transcriptional activity. The deletion of a 30-aa central region of DREB2A known as the negative regulatory domain (NRD) transforms DREB2A into a stable and constitutively active form referred to as DREB2A CA. However, the molecular basis of this stabilization and activation has remained unknown for a decade. Here we identified BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors of the Cullin3 (CUL3)-based E3 ligase, as DREB2A-interacting proteins. We observed that DREB2A and BPMs interact in the nuclei, and that the NRD of DREB2A is sufficient for its interaction with BPMs. BPM-knockdown plants exhibited increased DREB2A accumulation and induction of DREB2A target genes under heat and drought stress conditions. Genetic analysis indicated that the depletion of BPM expression conferred enhanced thermotolerance via DREB2A stabilization. Thus, the BPM-CUL3 E3 ligase is likely the long-sought factor responsible for NRD-dependent DREB2A degradation. Through the negative regulation of DREB2A stability, BPMs modulate the heat stress response and prevent an adverse effect of excess DREB2A on plant growth. Furthermore, we found the BPM recognition motif in various transcription factors, implying a general contribution of BPM-mediated proteolysis to divergent cellular responses via an accelerated turnover of transcription factors.

scholarly journals Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean

2016 ◽  
Vol 2016 ◽  
pp. 1-23 ◽  
Author(s):  
Aayudh Das ◽  
Moustafa Eldakak ◽  
Bimal Paudel ◽  
Dea-Wook Kim ◽  
Homa Hemmati ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Tolerance ◽  
Carbon Fixation ◽  
Calvin Cycle ◽  
Proteome Analysis ◽  
Tolerance Mechanisms ◽  
Heat Stress Response ◽  
Proteomic Data ◽  
Biochemical Analyses ◽  
Drought And Heat Stress

Drought and heat are among the major abiotic stresses that affect soybean crops worldwide. During the current investigation, the effect of drought, heat, and drought plus heat stresses was compared in the leaves of two soybean varieties, Surge and Davison, combining 2D-DIGE proteomic data with physiology and biochemical analyses. We demonstrated how 25 differentially expressed photosynthesis-related proteins affect RuBisCO regulation, electron transport, Calvin cycle, and carbon fixation during drought and heat stress. We also observed higher abundance of heat stress-induced EF-Tu protein in Surge. It is possible that EF-Tu might have activated heat tolerance mechanisms in the soybean. Higher level expressions of heat shock-related protein seem to be regulating the heat tolerance mechanisms. This study identifies the differential expression of various abiotic stress-responsive proteins that regulate various molecular processes and signaling cascades. One inevitable outcome from the biochemical and proteomics assays of this study is that increase of ROS levels during drought stress does not show significant changes at the phenotypic level in Davison and this seems to be due to a higher amount of carbonic anhydrase accumulation in the cell which aids the cell to become more resistant to cytotoxic concentrations of H2O2.

scholarly journals Tolerance of combined drought and heat stress is associated with transpiration maintenance and water soluble carbohydrates in wheat grains

2020 ◽  
Author(s):  
Abdeljalil El Habti ◽  
Delphine Fleury ◽  
Nathaniel Jewell ◽  
Trevor Garnett ◽  
Penny J. Tricker
Keyword(s):  
Heat Stress ◽  
Water Use ◽  
Vapour Pressure Deficit ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Plant Water ◽  
Plant Water Use ◽  
Whole Plant ◽  
Water Soluble Carbohydrates ◽  
Drought And Heat Stress

AbstractWheat (Triticum aestivum L.) production is increasingly challenged by simultaneous drought and heatwaves. We assessed the effect of both stresses combined on whole plant water use and carbohydrate partitioning in eight bread wheat genotypes that showed contrasting tolerance. Plant water use was monitored throughout growth, and water-soluble carbohydrates (WSC) and starch were measured following a three-day heat treatment during drought. WSC were predominantly allocated to the spike in modern Australian varieties, whereas the stem contained most WSC in older genotypes. Combined drought and heat stress increased WSC partitioning to the spike in older genotypes but not in the modern varieties. Glucose and fructose concentrations in grains measured 12 days after anthesis were associated with final grain weight in the main spike. At the whole plant level, combined drought and heat stress differentially altered daily water use and transpiration response to vapour pressure deficit during grain filling, compared to drought only. Final grain yield was increasingly associated with aboveground biomass and total water use with increasing stress intensity. Ability to maintain transpiration, especially following combined drought and heat stress, appears essential for maintaining wheat productivity.One sentence summaryHigher yield following drought and heat stress in wheats that maintain transpiration and have higher water-soluble carbohydrates content in grains.

Single Gene Consistently Associated with Heat Stress Response in Three Distinct Chicken Lines

2017 ◽  
Author(s):  
Xi Lan ◽  
John C. F. Hsieh ◽  
Carl J. Schmidt ◽  
Qing Zhu ◽  
Susan J. Lamont
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Single Gene ◽  
Heat Stress Response

The Heat Stress Response and Diabetes: More Room for Mitochondrial Implication

2016 ◽  
Vol 22 (18) ◽  
pp. 2619-2639 ◽  
Author(s):  
Biljana Miova ◽  
Maja Dimitrovska ◽  
Suzana Dinevska-Kjovkarovska ◽  
Juan V. Esplugues ◽  
Nadezda Apostolova
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Stress Response

scholarly journals Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bin Huang ◽  
Zhinuo Huang ◽  
Ruifang Ma ◽  
Jialu Chen ◽  
Zhijun Zhang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Shock ◽  
Gene Family ◽  
Regulatory Elements ◽  
Moso Bamboo ◽  
Naphthalene Acetic Acid ◽  
Plant Responses ◽  
Heat Stress Response ◽  
Regulatory Pathways

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

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