Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean

Drought and heat are among the major abiotic stresses that affect soybean crops worldwide. During the current investigation, the effect of drought, heat, and drought plus heat stresses was compared in the leaves of two soybean varieties, Surge and Davison, combining 2D-DIGE proteomic data with physiology and biochemical analyses. We demonstrated how 25 differentially expressed photosynthesis-related proteins affect RuBisCO regulation, electron transport, Calvin cycle, and carbon fixation during drought and heat stress. We also observed higher abundance of heat stress-induced EF-Tu protein in Surge. It is possible that EF-Tu might have activated heat tolerance mechanisms in the soybean. Higher level expressions of heat shock-related protein seem to be regulating the heat tolerance mechanisms. This study identifies the differential expression of various abiotic stress-responsive proteins that regulate various molecular processes and signaling cascades. One inevitable outcome from the biochemical and proteomics assays of this study is that increase of ROS levels during drought stress does not show significant changes at the phenotypic level in Davison and this seems to be due to a higher amount of carbonic anhydrase accumulation in the cell which aids the cell to become more resistant to cytotoxic concentrations of H2O2.

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Lipidome analysis of Symbiodiniaceae reveals possible mechanisms of heat stress tolerance in reef coral symbionts

Coral Reefs ◽

10.1007/s00338-019-01865-x ◽

2019 ◽

Vol 38 (6) ◽

pp. 1241-1253 ◽

Cited By ~ 5

Author(s):

S. Rosset ◽

G. Koster ◽

J. Brandsma ◽

A. N. Hunt ◽

A. D. Postle ◽

...

Keyword(s):

Climate Change ◽

Heat Stress ◽

Heat Tolerance ◽

Lipid Composition ◽

Elevated Temperatures ◽

Reef Coral ◽

Membrane Lipid ◽

Steady Increase ◽

Heat Stress Response ◽

Photosynthetic Organisms

Abstract Climate change-induced global warming threatens the survival of key ecosystems including shallow water coral reefs. Elevated temperatures can disrupt the normal physiological functioning of photosynthetic organisms by altering the fluidity and permeability of chloroplast membranes that is defined and regulated by their lipid composition. Since the habitat-forming reef corals rely on the obligatory symbiosis with dinoflagellates of the family Symbiodiniaceae, their heat stress response can be expected to be strongly influenced by the symbiont's lipid metabolism. However, in contrast to the steady increase in the knowledge of the functioning of coral symbionts at the genomic and transcriptomic level, the understanding of their membrane lipid composition and regulation in response to temperature stress is lagging behind. We have utilised mass spectrometry-based lipidomic analyses to identify the key polar lipids that form the biological membranes of reef coral symbionts, comparing the thermotolerant species Durusdinium trenchii with the thermosensitive taxon Cladocopium C3, both hosted by Acropora valida. Our results indicate that the superior thermotolerance D. trenchii inside the host corals could be achieved through (1) the amount and saturation of sulfoquinovosyldiacylglycerols, in particular through putative photosystem II interactions, (2) the increased digalactosyldiacylglycerol to monogalactosyldiacylglycerol ratio with the potential to stabilise thylakoid membranes and integrated proteins, and (3) the chaperone-like function of lyso-lipids. Thereby, our study provides novel insights into the heat tolerance of coral symbionts, contributing to the understanding of the potential of coral reef ecosystems to respond and adjust to heat stress events that are becoming more frequent due to climate change. Finally, our identification of multiple mechanisms of heat tolerance in Symbiodiniaceae furthers the knowledge of the general stress physiology of photosynthetic organisms.

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iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

10.21203/rs.3.rs-46970/v1 ◽

2020 ◽

Author(s):

Jing Wang ◽

Chengliang Liang ◽

Sha Yang ◽

Jingshuang Song ◽

Xuefeng Li ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Heat Tolerance ◽

Proteomic Analysis ◽

Molecular Mechanisms ◽

Negative Impact ◽

Growth And Yield ◽

Vegetable Crops ◽

Heat Stress Response ◽

Quantitative Proteomic Analysis

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1704189114 ◽

2017 ◽

Vol 114 (40) ◽

pp. E8528-E8536 ◽

Cited By ~ 23

Author(s):

Kyoko Morimoto ◽

Naohiko Ohama ◽

Satoshi Kidokoro ◽

Junya Mizoi ◽

Fuminori Takahashi ◽

...

Keyword(s):

Transcription Factors ◽

Heat Stress ◽

Target Genes ◽

Active Form ◽

E3 Ligase ◽

Protein Stabilization ◽

Regulatory Domain ◽

Heat Stress Response ◽

Element Binding Protein ◽

Drought And Heat Stress

DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A (DREB2A) acts as a key transcription factor in both drought and heat stress tolerance in Arabidopsis and induces the expression of many drought- and heat stress-inducible genes. Although DREB2A expression itself is induced by stress, the posttranslational regulation of DREB2A, including protein stabilization, is required for its transcriptional activity. The deletion of a 30-aa central region of DREB2A known as the negative regulatory domain (NRD) transforms DREB2A into a stable and constitutively active form referred to as DREB2A CA. However, the molecular basis of this stabilization and activation has remained unknown for a decade. Here we identified BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors of the Cullin3 (CUL3)-based E3 ligase, as DREB2A-interacting proteins. We observed that DREB2A and BPMs interact in the nuclei, and that the NRD of DREB2A is sufficient for its interaction with BPMs. BPM-knockdown plants exhibited increased DREB2A accumulation and induction of DREB2A target genes under heat and drought stress conditions. Genetic analysis indicated that the depletion of BPM expression conferred enhanced thermotolerance via DREB2A stabilization. Thus, the BPM-CUL3 E3 ligase is likely the long-sought factor responsible for NRD-dependent DREB2A degradation. Through the negative regulation of DREB2A stability, BPMs modulate the heat stress response and prevent an adverse effect of excess DREB2A on plant growth. Furthermore, we found the BPM recognition motif in various transcription factors, implying a general contribution of BPM-mediated proteolysis to divergent cellular responses via an accelerated turnover of transcription factors.

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A conserved HSF:miR169:NF-YA loop involved in tomato and Arabidopsis heat stress tolerance

10.1101/2021.01.01.425064 ◽

2021 ◽

Author(s):

Sombir Rao ◽

Chandni Bansal ◽

Celine Sorin ◽

Martin Crespi ◽

Saloni Mathur

Keyword(s):

Transcriptional Regulation ◽

Transcription Factors ◽

Heat Stress ◽

Stress Tolerance ◽

Heat Tolerance ◽

Critical Role ◽

Negative Regulator ◽

Virus Induced Gene Silencing ◽

Heat Stress Response ◽

Heat Stress Tolerance

AbstractHeat stress transcription factors (HSFs) and miRNAs regulate different stress and developmental networks in plants. Regulatory feedbacks are at the basis of these networks. Here, we report that plants improve their heat stress tolerance through HSF-mediated transcriptional regulation of MIR169 and post-transcriptional regulation of NF-YA transcription factors. We show that HSFs recognize tomato and Arabidopsis MIR169 promoters using yeast-one-hybrid/ChIP-qPCR. Silencing tomato HSFs using virus induced gene silencing (VIGS) reduced Sly-MIR169 levels and enhanced Sly-NF-YA9/A10 target expression. Further, Sly-NF-YA9/A10-VIGS knock-down tomato plants and Arabidopsis plants overexpressing At-MIR169d or At-nf-ya2 mutants showed a link with increased heat tolerance. In contrast, Arabidopsis plants overexpressing At-NF-YA2, or those expressing a non-cleavable At-NF-YA2 form (miR169d-resistant At-NF-YA2) as well as plants inhibited for At-miRNA169d regulation (miR169d mimic plants) were more sensitive to heat stress, highlighting NF-YA as negative regulator of heat tolerance. Furthermore, post-transcriptional cleavage of NF-YA by elevated miR169 levels resulted in alleviating the repression of heat stress effectors HSFA7a/b in tomato and Arabidopsis revealing a retroactive control of HSFs by the miR169:NF-YA node. Hence, a regulatory feedback loop involving HSFs, miR169s and NF-YAs plays a critical role in the regulation of heat stress response in tomato and Arabidopsis plants.

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Studies on the drought and heat stress response of green bean ( Phaseolus vulgaris L.) varieties under phytotronic conditions

Acta Agronomica Hungarica ◽

10.1556/aagr.56.2008.3.8 ◽

2008 ◽

Vol 56 (3) ◽

pp. 321-328 ◽

Cited By ~ 2

Author(s):

E. Nemeskéri ◽

J. Remenyik ◽

M. Fári

Keyword(s):

Heat Stress ◽

Control Level ◽

Water Soluble ◽

Green Beans ◽

Water Supplies ◽

Heat Stress Response ◽

Antioxidant Content ◽

Short Period ◽

Drought And Heat Stress ◽

Optimum Water

The drought tolerance of six green-and yellow-podded varieties of green beans with different genetic backgrounds was tested in the phytotron. During the week prior to flowering the plants were kept either at 25/15°C (day/night) or at high temperature (30/15°C), with RH 75% and optimum water supplies. The heat-stressed plants were then divided into three groups; the first was returned to the control (25/15°C) chamber (RH 75%, optimum water supplies), while the second and third were exposed to mild drought stress (RH 60%, 50% water) at temperatures of 30/15°C and 35/25°C, respectively, throughout the flowering period.The varieties survived the short period of heat stress (30/15°C) prior to flowering without damage provided the temperature during flowering was reduced to 25/15°C and the water supplies were optimum. There was a sharp increase in the carotene level in the leaves of drought-stressed plants when the temperature during flowering was 30/15°C, but in plants exposed to 35/25°C during flowering the level dropped to near the control level. The latter group exhibited considerable damage, with a reduction in the water-soluble antioxidant content (ACW: antioxidant capacity of water-soluble substances) and the chlorophyll b content compared with the control.The antioxidant content (ACW) in the dark green leaves of green-podded varieties was lower than in the yellow-podded varieties and did not change as the result of drought and heat stress. In yellow-podded varieties, however, there was a significant decline in ACW in response to stress. Differences between the varieties in their adaptability to drought and heat could be detected as changes in the chlorophyll and carotene contents of the leaves even at 30/15°C.

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Differential Responses of Hybrid Bluegrass and Kentucky Bluegrass to Drought and Heat Stress

HortScience ◽

10.21273/hortsci.43.7.2191 ◽

2008 ◽

Vol 43 (7) ◽

pp. 2191-2195 ◽

Cited By ~ 10

Author(s):

Eleni M. Abraham ◽

William A. Meyer ◽

Stacy A. Bonos ◽

Bingru Huang

Keyword(s):

Heat Stress ◽

Heat Tolerance ◽

Transpiration Rate ◽

Soil Depth ◽

Poa Pratensis ◽

Kentucky Bluegrass ◽

Optimal Temperature ◽

Turf Quality ◽

Differential Responses ◽

Drought And Heat Stress

This study was designed to investigate differential responses of hybrids from Texas bluegrass (Poa arachnifera Torr.) × Kentucky bluegrass (Poa pratensis L.) (KBG) and KBG genotypes to drought and heat stress. Plants of two hybrids, ‘845’ and ‘BDF’, and two KBG genotypes (‘Midnight’ and ‘C-74’) were grown under optimal temperature (22/18 °C) and well-watered (control) or unwatered (drought) or superoptimal temperatures (35/30 °C; heat stress) conditions for 35 days in growth chambers. Under optimal conditions, the two hybrids and two KBG genotypes were not significantly different in turf quality, leaf photochemical efficiency expressed as chlorophyll fluorescence ratio (Fv/Fm), leaf net photosynthetic rate (Pn), transpiration rate, water use efficiency (WUE), root dry matter, or root viability. The results suggest that the interspecific hybridization resulted in similar growth and physiological traits in the hybrid bluegrass as in a turf-type species under optimal temperature and irrigation regimes. Under drought stress, all these parameters were comparable to those for KBG ‘Midnight’, but significantly higher than the corresponding parameters for KBG ‘C-74’. Under heat stress, both hybrids had significantly higher turf quality, Fv/Fm, Pn, transpiration rate, WUE, root dry weight in deeper soil depth (40 to 60 cm), and root viability in the upper 40-cm layer compared with both KBG genotypes. These results suggested that hybrid bluegrass exhibited improvement in drought and heat tolerance, particularly in comparison with KBG ‘C-74’, but to a great extent for heat tolerance. The maintenance of higher transpiration and photosynthesis, WUE, and root viability was associated with the improvement in heat tolerance in hybrid bluegrass.

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