Effects of seed quality on the proportion of seed transmission forSugarcane mosaic virusin maize

2011 ◽  
Vol 39 (2) ◽  
pp. 257-266 ◽  
Author(s):  
L. Li ◽  
X. Wang ◽  
G. Zhou
Keyword(s):  
Seed Quality ◽  
Seed Transmission

scholarly journals Role of Soybean mosaic virus–Encoded Proteins in Seed and Aphid Transmission in Soybean

2013 ◽  
Vol 103 (9) ◽  
pp. 941-948 ◽  
Author(s):  
Sushma Jossey ◽  
Houston A. Hobbs ◽  
Leslie L. Domier
Keyword(s):  
Mosaic Virus ◽  
Seed Quality ◽  
Soybean Mosaic Virus ◽  
Seed Transmission ◽  
Aphid Transmission ◽  
Plant Introduction ◽  
Sequence Motif ◽  
Coding Region ◽  
Encoded Proteins ◽  
Seed Transmissibility

Soybean mosaic virus (SMV) is seed and aphid transmitted and can cause significant reductions in yield and seed quality in soybean (Glycine max). The roles in seed and aphid transmission of selected SMV-encoded proteins were investigated by constructing mutants in and chimeric recombinants between SMV 413 (efficiently aphid and seed transmitted) and an isolate of SMV G2 (not aphid or seed transmitted). As previously reported, the DAG amino acid sequence motif near the amino terminus of the coat protein (CP) was the major determinant in differences in aphid transmissibility of the two SMV isolates, and helper component proteinase (HC-Pro) played a secondary role. Seed transmission of SMV was influenced by P1, HC-Pro, and CP. Replacement of the P1 coding region of SMV 413 with that of SMV G2 significantly enhanced seed transmissibility of SMV 413. Substitution in SMV 413 of the two amino acids that varied in the CPs of the two isolates with those from SMV G2, G to D in the DAG motif and Q to P near the carboxyl terminus, significantly reduced seed transmission. The Q-to-P substitution in SMV 413 also abolished virus-induced seed-coat mottling in plant introduction 68671. This is the first report associating P1, CP, and the DAG motif with seed transmission of a potyvirus and suggests that HC-Pro interactions with CP are important for multiple functions in the virus infection cycle.

scholarly journals The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis

2021 ◽  
Author(s):  
Meral Yılmaz ◽  
Ömür Baysal ◽  
Ragıp Soner Silme
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Seed Quality ◽  
Seed Transmission ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Clavibacter Michiganensis ◽  
Origanum Vulgare ◽  
Ligand Interaction ◽  
Clavibacter Michiganensis Subsp

The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential oil (EO) against Clavibacter michiganensis subsp. michiganensis (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande) coated with different doses of EO derived from O. vulgare were inoculated with Cmm (1.8 × 10<sup>8</sup> CFU/mL). O. vulgare's EO showed a remarkable inhibition effect on the Cmm growth. The EO effect against Cmm was determined based on the parameters, such as the inhibition zone and bacterial population in a seed. The GC-MS analysis of EO showed that carvacrol is the major component (at 74.05%), which may inhibit the bacterial growth. Later, we have expanded our studies to determine the inhibitory effect of the EO's mode of action on the pathogenic bacteria with a molecular docking analysis based on the molecular protein-ligand interaction. The results showed that carvacrol has a strong interaction with the bacterial expansin protein (PDB 4JJO) of Cmm and the qPCR analyses confirmed the effect of the O. vulgare treatment against Cmm. This original approach has the prominent potential to prevent seed transmission of Cmm for seed quality in the world, suggesting a method for paving the way for Cmm disease management.

Further studies on Pea seed-borne mosaic virus in cool-season crop legumes: responses to infection and seed quality defects

2008 ◽  
Vol 59 (12) ◽  
pp. 1130 ◽  
Author(s):  
B. A. Coutts ◽  
R. T. Prince ◽  
R. A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Seed Coat ◽  
Faba Bean ◽  
Seed Quality ◽  
Broad Bean ◽  
Seed Transmission ◽  
Field Pea ◽  
Cool Season ◽  
Moderately Resistant ◽  
Pea Seed

Field and glasshouse experiments (3 of each) were done during 2003–06 to determine the responses of a range of genotypes belonging to 13 species of cool-season crop legumes to infection with Pea seed-borne mosaic virus (PSbMV). Seed quality defects were determined and genotypes of some species were also tested for seed transmission of the virus. In field experiments, of 39 genotypes of field pea (Pisum sativum) evaluated, 15 were ranked as highly susceptible, 10 susceptible, 9 moderately resistant, and 5 resistant, while all 7 lupin species (Lupinus spp.) tested were resistant. In glasshouse sap and graft inoculations with PSbMV to genotypes not found infected in the field and 2 additional lupin species, no virus was detected in any of the 9 lupin species or in 5 field pea genotypes tested. Thus, the lupins all appeared to be non-hosts and the 5 field pea genotypes had resistance to the 2 PSbMV isolates used to inoculate them. All 14 genotypes of faba bean (Vicia faba) evaluated in the field were ranked highly susceptible, while 12 out of 16 lentil (Lens culinaris) genotypes were ranked as highly susceptible and 4 as susceptible. Chickpea (Cicer arietinum) genotypes were moderately resistant (50) or susceptible (7). Once infected, plant sensitivities (symptom severities) ranged from low in some field pea and most lentil genotypes to high in most faba bean genotypes. Chickpea genotypes all were ranked as moderately sensitive. Seed lots harvested from PSbMV-infected plants of field pea, faba bean, and chickpea all showed severe seed quality defects, but lentil was usually less affected. The predominant seed symptoms were necrotic rings and line markings on the seed coat, malformation, reduced size, and splitting. Kabuli chickpea types also showed darkening of the seed coat. Seed transmission of PSbMV was detected in faba bean (0.2%) and field pea (5–30%). When PSbMV infection foci were introduced into plots of lentil cv. Nugget, the virus spread to the lentil plants and decreased shoot dry weight by 23%, seed yield by 96%, and individual seed weight by 58%. Seed transmission of PSbMV (6%) was detected in seed from the infected lentil plants. In a survey for possible viral seed symptoms, all seed lots of kabuli chickpea (5) and field pea (70), and 10 of 18 of faba bean were affected, but none of the 23 of lentil. When seedlings from 16 faba bean and 7 field pea seed lots were tested for 3 viruses, neither Broad bean stain virus nor Broad bean true mosaic virus was detected, but PSbMV was found in 5 field pea seed lots at incidences of <1–14%. PSbMV was detected in commercial field pea seed stocks of cvv. Kaspa (33) and Parafield (12) at incidences of 0.5–47% and 0.3–30%, respectively. The implications of these findings in terms of genotype susceptiblility and sensitivity to PSbMV infection and their importance for the management of PSbMV in legume crops are discussed.

scholarly journals Wet Seed Treatment with Peroxyacetic Acid for the Control of Bacterial Fruit Blotch and Other Seedborne Diseases of Watermelon

Plant Disease ◽  
2003 ◽  
Vol 87 (12) ◽  
pp. 1495-1499 ◽  
Author(s):  
D. L. Hopkins ◽  
C. M. Thompson ◽  
J. Hilgren ◽  
B. Lovic
Keyword(s):  
Seed Treatment ◽  
Seed Quality ◽  
Treatment Protocol ◽  
Seed Transmission ◽  
Effective Control ◽  
Peroxyacetic Acid ◽  
Didymella Bryoniae ◽  
Bacterial Fruit Blotch ◽  
Low Concentrations ◽  
Triploid Watermelon

Prevention of seed transmission of Acidovorax avenae subsp. citrulli into the transplant house or field is the most effective control of bacterial fruit blotch of watermelon currently available. Peroxyacetic acid was evaluated as a disinfectant that might efficaciously eradicate A. avenae subsp. citrulli from contaminated seed and also be efficacious against other seed-transmitted diseases of watermelon. Peroxyacetic acid at low concentrations eliminated A. avenae subsp. citrulli, Fusarium oxysporum, and Didymella bryoniae from microbial suspensions. Treatments of seed contaminated with A. avenae subsp. citrulli and D. bryoniae with peroxyacetic acid at 1,600 μg/ml and higher for 30 min were effective in preventing seed transmission of bacterial fruit blotch and gummy stem blight. Hydrochloric acid treatments at 10,000 μg/ml, while effective in eliminating seed transmission to watermelon seedlings, can adversely affect seed germination, especially with triploid seed. Efficacious dosages of peroxyacetic acid can be applied safely to freshly harvested triploid watermelon seed without concerns for reduction in seed quality. A most effective wet seed treatment protocol involved a 30-min treatment with peroxyacetic acid at 1,600 μg/ml followed by seed drying at low humidity in a 40°C drying oven for 48 h.

↵​Urdbean Leaf Crinkle Disease- Assessment of Seed Transmissibility and Its Effect on Yield and Seed Quality in Urdbean [Vigna mungo (L.) Hepper]

2021 ◽  
Author(s):  
T. Priyanga ◽  
T.K.S. Latha ◽  
T. Ramya teja ◽  
G. Karthikeyan ◽  
K. Prabakar
Keyword(s):  
Yield Loss ◽  
Seed Quality ◽  
Seed Transmission ◽  
Quality Parameters ◽  
Vigna Mungo ◽  
Stress Factors ◽  
Disease Assessment ◽  
Seed Transmissibility ◽  
Effect On Yield ◽  
Biotic Stress Factors

Background: Urdbean leaf crinkle disease (ULCD) is one of the most devastating biotic stress factors which is responsible for drastic yield loss in urdbean [Vigna mungo (L.) Hepper]. The etiology of ULCD is still unknown and there are conflicting reports on its transmission through seed, insect vectors, grafting and infected sap. Methods: Per cent seed transmission of ULCD had been studied during kharif, rabi and summer during the year 2019-2020 under natural and insect proof conditions. The effect on yield and seed quality parameters due to ULCD infection was studied. Urdbean cultivar VBN 8 was used in the present study. Result: The per cent seed transmission of ULCD was recorded ranging from 52.88 to 71.64% under natural conditions. In the insect proof glasshouse condition, the seed transmission was 83.62%. All the yield and seed quality parameters were adversely affected upon ULCD infection. The reduction in yield was recorded as 64.36% over the uninfected healthy plants. In ULCD infected seeds, the germination was reduced (84%) as compared to healthy seeds (92%).

MANAGEMENT OF NEW CULTIVARS FOR DRYLAND

1983 ◽  
pp. 61-73 ◽  
Author(s):  
J.A. Lancashire ◽  
J.L. Brock
Keyword(s):  
Tall Fescue ◽  
Seed Quality ◽  
Technical Information ◽  
Grazing Management ◽  
Grass Species ◽  
Hill Country ◽  
Seasonally Dry ◽  
Prairie Grass ◽  
Management Procedures ◽  
New Cultivars

Some characteristics of seed quality, establishment rates, performance in mixtures and response to grazing management of 5 new pasture plants with potential in dryland are described. On a dry hill country site in the Wairarapa, the contribution of the sown grasses established in separate plots with clovers under rotational grazing was 'Grasslands Wana' cocksfoot 65%; 'Grasslands Maru' phalaris 23%; 'Grasslands Matua' prairie grass 22%; and 'Grasslands Roa' tall fescue 13% after 2 years. The other main grass species was resident perennial ryegrass which established from buried seed (ca. 240 plants/m*) and had a major impact on the establishment and growth of the sown grasses. On a seasonally dry Manawatu flat land soil 3 grazing managementsviz. set stocked all year (S); rotational all year (R); and combination (Cl (set stocked from lambing to drafting and rotational for the remainder of the year) were applied to mixtures of the new cultivars (except that 'Grasslands Apanui' cocksfoot replaced Wana) with ryegrass and white clover stocked at 20 sheep/ha. After 3 years the contribution of the new cultivars was negligible under S and ryegrass was dominant. The R pastures became cocksfoot dominant and Matua (in winter) and chicory (in summer) contributed more than in the S system. The C system produced the most evenly balanced species contribution with only Roa remaining at (5%. A sub-trial with cocksfoot cultivars demonstrated that Wana maintained better production and tiller density ~ll,000/m2 ) than Apanui (1000/m' ) under set stocking IS). Although some of the new cultivars will require specialised management procedures to fulfil their potential in dryland, the increasing and widespread use of Matua prairie grass in farming suggests that these techniques can be adopted in commercial agriculture provided good technical information is available in a management package when the cultivar is released. Keywords: Dryland, grazing management, mixtures, Matua prairie grass, Wana cocksfoot, Roa tall fescue, Maru phalaris, Chicory

Yield and Seed quality of barley (Hordeum vulgare L.) as affected by variety, nitrogen level and harvesting time

2014 ◽  
Vol 2 (2) ◽  
pp. 93-100
Author(s):  
Shahnaj Yesmina ◽  
Moushumi Akhtarb ◽  
Belal Hossain
Keyword(s):  
Grain Yield ◽  
Seed Weight ◽  
Seed Quality ◽  
Quality Parameters ◽  
Nitrogen Level ◽  
Yield Attributes ◽  
Harvesting Time ◽  
Nitrogen Levels ◽  
Vigour Index

The experiment was conducted to find out the effect of variety, nitrogen level and harvesting time on yield and seed quality of barley. The treatments used in the experiment consisted of two varieties viz. BARI Barley 4 and BARI Barley 5, three harvesting time viz. 35, 40 and 45 Days after Anthesis (DAA) and nitrogen levels viz. 0, 70, 85 and 100 kg N ha-1 . The experiment was laid out in a spilt- spilt-plot design with three replications assigning the variety to the main plot, harvesting time to the sub-plots and nitrogen level to the sub-sub plots. Variety had significant effects on the all yield attributes except fertile seeds spike-1 . Seed quality parameters viz. normal seeds spike-1 , deformed seeds spike-1 , germination (%) and vigour index were statistically significant. The variety BARI Barley 5 produced higher grain yield and seed quality than BARI Barley 4. Grain yield from BARI Barley 5 and BARI Barley 4 were 4.59 t ha-1 and 4.24 t ha-1 , respectively. Significantly, the highest 1000-seed weight (46.90 g) was produced by BARI Barley 5 than (37.90 g) BARI Barley 4. The result revealed that harvesting time had significant effect on yield and yield attributes and seed quality parameters. Seed yield was highest (4.65 t ha-1 ) when the crop harvested at 40 DAA and it was increased linearly from 35 DAA. Maximum quality seed and 1000-seed weight (43.20 g) was obtained when the crop harvested at 40 DAA. All the yields, yield attributes and seed quality parameters were significantly influenced by nitrogen levels. The highest grain yield (5.14 t ha-1 ) was obtained when BARI Barley 5 variety was fertilized by 100 kg N ha-1 and the lowest (3.14 t ha-1 ) was obtained from control treatments. Normal seeds spike-1 , vigour index, germination (%) were better at 85 kg N ha-1 in variety of BARI Barley 5 than BARI Barley 4. So it can be concluded that BARI Barley 5 showed better result when fertilized with 100 kg N ha-1 and harvested at 40 DAA for getting maximum yield and 85 kg N ha-1 and harvested at 40 DAA for getting better quality seed.

Цифровая морфометрия разнокачественности семян овощных культур

2018 ◽  
Author(s):  
F.B. Musaev ◽  
N.S. Priyatkin ◽  
M.V. Arkhipov ◽  
P.A. Shchukina ◽  
A.F. Bukharov ◽  
...  
Keyword(s):  
Seed Quality ◽  
Image Analysis System ◽  
Flatbed Scanner ◽  
The Future ◽  
Scanned Images ◽  
Computer Morphometry ◽  
Analysis System ◽  
Matrix Heterogeneity ◽  
Relationship Of ◽  
Selection Of

Приведено описание разработанной авторами методики цифровой компьютерной морфометрии семян овощных культур на основе системы анализа изображений, состоящей из планшетного сканера и программного обеспечения для автоматических измерений. В основу метода положено представление о разнокачественности семян, обусловленной генетической неоднородностью самих семенных растений, используемых в промышленном семеноводстве. Физические свойства семян (их форма и линейные размеры) – основные параметры при определении их качества. Цифровые изображения семян получены при помощи планшетного сканера HP Sсanjet 200 на базе Агрофизического НИИ с использованием серийного программного обеспечения «Argus-BIO», производства ООО «АргусСофт» (г. Санкт-Петербург). Метод состоит из подбора контрастной подложки (фона) для сканирования семян с минимальными теневыми эффектами, калибровку программного обеспечения для привязки к истинным размерным величинам, подбор параметров измерений и автоматическое распознавание цифровых сканированных изображений семян. Представлены экспериментальные данные по морфометрии экологически разнокачественных семян фасоли овощной, матрикально разнокачественных семян укропа, пастернака и лука Кристофа. Семена укропа и пастернака, собранные из разных порядков ветвления семенного растения, значительно различались по величине линейных параметров. Наиболее показательный линейный параметр семян – площадь проекции. Предложенная авторами методика цифровой морфометрии, уже использована на практике и в перспективе может быть задействована в исследованиях экологической и матрикальной разнокачественности семян овощных культур. Так, она прошла апробацию на разнокачественных семенах пяти сортов фасоли овощной (Настена, Магура, Миробела, Морена, Бажена) полученных в пяти контрастных эколого-географических условиях среды (Москва, Белгород, Ставрополь, Омск, Горки) в 2011–2012 годах. В дальнейшем методика может быть использована для улучшения качества цифровых изображений семян, изучения разнокачественности семян в том числе и для совершенствования контроля за селекционным процессом. Кроме того, она применима для изучения взаимосвязи совокупности морфометрических характеристик семян и их посевных качеств.The description of the method of digital computer morphometry of vegetable seeds developed by the authors on the basis of the image analysis system consisting of a flatbed scanner and software for automatic measurements is given. The method is based on the idea of seed quality, due to the genetic heterogeneity of the seed plants used in industrial seed production. Physical properties of seeds (their shape and linear dimensions) are the main parameters in determining their quality. Digital image of the seed obtained using the flatbed scanner, HP Sсanjet 200 on the basis of the Agrophysical research Institute with serial software “Argus-BIO”, produced by LLC “Argussoft” (Saint-Petersburg). The method consists of selection of a contrast substrate (background) for scanning seeds with minimal shadow effects, calibration of software for binding to true size values, selection of measurement parameters and automatic recognition of digital scanned images of seeds. Experimental data on the morphometry of ecologically different-quality seeds of vegetable beans, matrix seeds of dill, Pasternak and Christoph onion are presented. Seeds of dill and parsnip, collected from different orders of branching of the seed plant, significantly differed in size of linear parameters. The most revealing linear parameter seed – area projection. The method of digital morphometry proposed by the authors has already been used in practice and in the future can be used in studies of ecological and matrix heterogeneity of vegetable seeds. So, it was tested on different quality seeds of five varieties of vegetable beans (Nastena, Magura, Mirobelа, Morena, Bazhenf) obtained in five contrasting environmental and geographical conditions (Moscow, Belgorod, Stavropol, Omsk, Gorki) in 2011-2012. In the future, the technique can be used to improve the quality of digital images of seeds, study of seed diversity, including to improve the control of the breeding process. In addition, it is applicable to study the relationship of the set of morphometric characteristics of seeds and their sowing qualities.

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