scholarly journals Egg-lying behavior of Anisopteromalus calandrae (Howard), an Ectoparasitoid of Lasioderma serricorne (Fabricius)

2017 ◽  
Vol 39 (4) ◽  
pp. 416-420
Author(s):  
Nguyen Thi Oanh ◽  
Tran Ngoc Lan ◽  
Truong Xuan Lam
Keyword(s):  
Larval Instar ◽  
Egg Laying ◽  
Host Larva ◽  
Lasioderma Serricorne ◽  
Maximum Lifespan ◽  
Laboratory Conditions ◽  
Anisopteromalus Calandrae ◽  
Aqua Feed

This paper describes the egg-laying behavior and reproduction capacity of Anisopteromalus calandrae in laboratory conditions. Anisopteromalus calandrae lay eggs only on the Lasioderma serricorne larvae hiden inside of grains or nests. The maximum lifespan of A. calandrae females was 32 days when they were kept with of aqua feed flour plus honey (30%) and infested with larvae of Lasioderma serricorne. One female laid 71.13 ± 4.24 eggs. The eggs were laid mostly (81.95%) during the first half of the lifespan, with the peak of 7.40 eggs/day on the 9th day. The females usually lay one egg/host larva, but sometimes they lay 2-5 eggs/host larva. They prefer to lay eggs on the 4th larval instar. Citation: Nguyen Thi Oanh, Tran Ngoc Lan, Truong Xuan Lam, 2017. Egg-lying behavior of Anisopteromalus calandrae (Howard), an ectoparasitoid of Lasioderma serricorne (Fabricius). Tap chi Sinh hoc, 39(4): 416-420. DOI: 10.15625/0866-7160/v39n4.10935.* Corresponding author: [email protected] 17 August 2017, accepted 20 November 2017

Reproduction in Aplysia californica: correlations between egg laying in vivo and egg release in vitro

1980 ◽  
Vol 58 (11) ◽  
pp. 2163-2166 ◽  
Author(s):  
F. Edward Dudek ◽  
Amd Bonnie Soutar ◽  
Stephen S. Tobe
Keyword(s):  
Aplysia Californica ◽  
Egg Laying ◽  
Previous Episode ◽  
Laboratory Conditions ◽  
Release In Vitro

Aspects of egg laying by isolated Aplysia californica and egg release from ovotestis fragments were compared under laboratory conditions. The volume of eggs laid per episode increased as a function of time since the previous episode of egg laying. Egg output in vivo and egg release in vitro were maximal in autumn and minimal in spring, but a factor in the parietovisceral ganglion evoked egg release from ovotestis fragments throughout the year. These data are consistent with previous studies which have suggested that the effects of season and egg-laying history on egg laying involve substantial changes in the ovotestis.

scholarly journals The efficiency of Paecilomyce farinosus as biocontrol to larve stage on second ad sixith instar larve of Trogoderma granarium (Everts)

2013 ◽  
Vol 10 (4) ◽  
pp. 1096-1101
Author(s):  
Baghdad Science Journal
Keyword(s):  
Larval Instar ◽  
The Other ◽  
Biocontrol Agents ◽  
Larval Instars ◽  
Paecilomyces Farinosus ◽  
Trogoderma Granarium ◽  
Laboratory Conditions ◽  
Other Hand ◽  
Laboratory Results ◽  
Treatment Concentration

The present study was conducted to determine the pathogenicity of Paecilomyces farinosus as biocontrol agents against Tragoderma granarium (khapra) under laboratory conditions with three concentrations ( 2.8 x 108 , 2.8 x 106 ,2.8 x104 spores/ ml). The laboratory results revealed that fungi showed it's higher pathogencity to larve stage on 2nd instar and 6th instar , although their capability differs according to the fungi concentrations this fungi caused higher mortality of 2nd larval instar 70% and 60% ,55% when it was used at concentrations 2 .8 x 106 ,2.8 x104 spores/ ml respectively. On the other hand on 6th larval instars 55% , 35% , 30% at concentrations ( 2.8 x 108 , 2.8 x 106 ,2.8 x104 spores/ ml) respectively, and the highest rate of distortions in the adult insects from the 2nd instar treatment than the adult insects from the 6th instar treatment concentration of this fungi.

scholarly journals Biology of Anicla infecta (Ochsenheimer, 1816) (Lepidoptera, Noctuidae, Noctuinae), under laboratory conditions

2001 ◽  
Vol 61 (4) ◽  
pp. 661-666 ◽  
Author(s):  
J. A. TESTON ◽  
A. SPECHT ◽  
E. CORSEUIL
Keyword(s):  
Relative Humidity ◽  
Standard Error ◽  
Lolium Multiflorum ◽  
Economic Importance ◽  
Egg Laying ◽  
Adult Longevity ◽  
Environmental Chamber ◽  
Laboratory Conditions ◽  
The Mean ◽  
Lepidoptera Noctuidae

Larvae of Anicla infecta (Ochsenheimer, 1816) (Noctuidae) feed upon many grasses and may be harmful to cereals and fodder of economic importance. This study was developed aiming to contribute to knowledge of the biology of this species. The rearing was done in an environmental chamber with the following settings: temperature of 25 ± 1ºC; relative humidity of 70% <FONT FACE=Symbol>±</FONT> 10%, and photoperiod of L14: D10. The larvae fed on ryegrass, Lolium multiflorum Lam. The results express the mean and standard error for the length of every stage in days. For each stage we observed the following time of development: egg 3.2 <FONT FACE=Symbol>±</FONT> 0.09; larvae 18.7 <FONT FACE=Symbol>±</FONT> 0.07; pre-pupae 3.3 <FONT FACE=Symbol>±</FONT> 0.04; pupae 12.6 <FONT FACE=Symbol>±</FONT> 0.14; and adult longevity was 12.1 <FONT FACE=Symbol>±</FONT> 1.03. Also the pre-egg-laying period was 4.4 <FONT FACE=Symbol>±</FONT> 0.59; the egg-laying period was 8.1 <FONT FACE=Symbol>±</FONT> 0.84; and the post-egg-laying period was 0.3 <FONT FACE=Symbol>±</FONT> 0.14. The mean number of egg-laying cycles per female was 6.7 <FONT FACE=Symbol>±</FONT> 0.73; that of eggs per cycle was 77.5 <FONT FACE=Symbol>±</FONT> 4.37; and total eggs per female was 521.4 <FONT FACE=Symbol>±</FONT> 47.36.

scholarly journals Rearing of Maize Stem Borer, Chilo partellus (Lepidoptera: Crambidae) under Laboratory Conditions

2021 ◽  
Vol 2 (3) ◽  
pp. 22-26
Author(s):  
Muhammad Ijaz ◽  
Keyword(s):  
Chilo Partellus ◽  
Insect Pests ◽  
Agricultural Research ◽  
Larval Instar ◽  
Stem Borer ◽  
Raw Material ◽  
Research Centre ◽  
Maize Production ◽  
Laboratory Conditions ◽  
The Mean

Maize is an important cereal crop grown for food, fodder and raw material throughout the world. It is rick source of proteins, carbohydrates and vitamins. Maize production is decreasing day by day due to attacked of more than 250 insect species and mites. Among insect pests, maize stem borer, Chilo partellus is most destructive pest for maize production. The rearing of maize stem borer, C. partellus was conducted under laboratory conditions in National Agricultural research Centre (NARC) Islamabad. The complete metamorphosis (egg, larva, pupa and adult) was recorded in C. partellus. The mean value of egg was 4.67 ± 1.09 days. There were six larval instar of C. partellus and total larval period was 18-48 days. The growth period of first, second, third, fourth, fifth and sixth larval instar was 3-6, 2-6, 3-7, 3-8, 3-9 and 5-11 days respectively on maize leaves. The mean average duration of pupa was 9.00±2.25 days. The female was long lived than male. The total developmental period from egg to adult was 30-65 days.

scholarly journals Distribution, bioecological peculiarities of staphylinids (Coleoptera, Staphylinidae) in livestock biocenoses of forest-steppe and steppe Ukraine

2020 ◽  
Vol 28 (1) ◽  
pp. 24-28
Author(s):  
A. P. Palyi ◽  
A. N. Makshei ◽  
O. I. Kasianenko ◽  
R. V. Petrov ◽  
L. I. Faly ◽  
...  
Keyword(s):  
Species Diversity ◽  
Species Composition ◽  
Temperature Regime ◽  
Egg Laying ◽  
Forest Steppe ◽  
Large Species ◽  
Larval Stages ◽  
Laboratory Conditions ◽  
Feeding Specialization ◽  
Food Consumed

As a result of research in the territory of livestock farms, 103 species of predatory Coleoptera were found, including Staphylinidae accounting for 51.4%, Histeridae – 27.3%, Carabidae – 21.3%. A total of 39 species of the Staphylinidae family were identified, belonging to 5 subfamilies: Oxytelinae, Steninae, Staphylininae, Tachyporinae, Aleocharinae. Species composition of staphylinids varied in relation to the types of animal rearing premises. In cowsheds and calf pens species diversity was higher (35 species). To study the peculiarities of feeding in laboratory conditions, we monitored 9 species of Staphylinidae: Philonthus addendus Sharp, Ph. cruentatus (Gmel.), Ph. rectangulus Sharp, Ph. varians (Payk.), Ph. spinipes Sharp, Ph. nitidus F., Creophilus maxillosus (L.), Ontholestes murinus (L.), Oxytelus sp. Feeding specialization of imagoes and larvae of different ages was studied. We determined that the mass of food consumed per day is higher than the weight of the beetles. Large species of staphylinids prefer feeding on average-aged larvae of flies and can eat puparia. Peculiarities of the development of coprophilous staphylinids were studied on the example of Ph. spinipes Sharp. During 24 h a female laid 1–3 eggs (in +28°С). Duration of the egg phase depends on the temperature regime (in +20 °С – 4–5, +28 °С – 3–4 days). Cessation of egg laying was observed with decrease in the temperature to +14 °С. The development takes part in three larval stages. Duration of the larva phase at +28 °С was 8–10 days, at +20 °С – 13–14 days. The first moulting was observed on the 2–3th days (in +28 °С) after the larvae emerged from the eggs. At +28 °С the pupa phase lasted 8–10 days. Decrease in temperature prolonged the rate of the development. At +24 °С it practically did not change (9–10 days), at +18 °С – increased to 13–15 days.

scholarly journals Evaluation of Five Medicinal Plant Extracts on Aphis craccivora (Hemiptera: Aphididae) and Its Predator, Chrysoperla carnea (Neuroptera: Chrysopidae) under Laboratory Conditions

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 398 ◽  
Author(s):  
Samy M. Sayed ◽  
Saqer S. Alotaibi ◽  
Nevien Gaber ◽  
Sayed-Ashraf Elarrnaouty
Keyword(s):  
Hplc Analysis ◽  
Larval Instar ◽  
Aphis Craccivora ◽  
Production Costs ◽  
Botanical Insecticides ◽  
Chrysoperla Carnea ◽  
Lc50 Value ◽  
Laboratory Conditions ◽  
Methanolic Extracts ◽  
Medicinal Plant Extracts

Botanical insecticides that degrade rapidly are safer than persistent synthetic chemical insecticides, less harmful to the environment, decrease production costs and are not likely to cause insecticide resistance among pests. This study aimed to evaluate the effect of five different botanical extracts on the bean aphid, Aphis craccivora and the 2nd larval instar of the green lacewing, Chrysoperla carnea under laboratory conditions. Also, the flavonoids in the methanolic extracts of these tested plants were detected using HPLC analysis. The data from the HPLC analysis indicated that the tested plants differed in their flavonoid components. The total flavonoids were 869.4, 1125.6, 721.4, 1667.8 and 2025.9 mg/kg in Psiadia penninervia, Salvia officinalis, Ochradenus baccatus, Pulicaria crispa and Euryops arabicus, respectively. Moreover, there were many variations among these plants in the amount of each compound. The lethal concentration (LC50) value of P. penninervia extract on aphids was the lowest among all of the plants (128.546 µg/mL) followed by O. baccatus (626.461 µg/mL). Also, the LC50 value of P. penninervia extract on the 2nd larval instar of C. carnea (232.095 µg/mL) was significantly lower than those of all other four plant species extracts, while the other four plants did not show significant differences among them according to relative median potency analyses. Accordingly, O. baccatus extract had a strong effect on aphids and was safest for the predator. This finding suggests that O. baccatus could be exploited and further developed as an effective plant extract-based insecticide to be utilized in integrated pest management (IPM) programs against A. craccivora.

Reproductive traits ofMonochamus galloprovincialis(Coleoptera: Cerambycidae) under laboratory conditions

2006 ◽  
Vol 96 (3) ◽  
pp. 289-294 ◽  
Author(s):  
P. Naves ◽  
E. de Sousa ◽  
J.A. Quartau
Keyword(s):  
Secondary Forest ◽  
Reproductive Potential ◽  
Principal Component ◽  
Reproductive Traits ◽  
Bursaphelenchus Xylophilus ◽  
Egg Laying ◽  
Large Individual ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Laboratory Conditions

AbstractThe pine sawyerMonochamus galloprovincialis(Olivier) is the vector of the introduced pine wood nematodeBursaphelenchus xylophilus(Steiner & Bührer) Nickle in Portugal, and until recently was considered a secondary forest insect. Under laboratory conditions, a study of biological and reproductive traits of 37 insect pairs was conducted. The longevity of both sexes was similar, being 61.2±6.5 days for males and 64.0±6.3 days for females (mean±SE). Sixteen small-sized insects (22% of the population) died within 20 days and before starting to reproduce. The sexual maturation period (without egg laying) was 20.4±0.7 days (mean±SE), while the oviposition period lasted 54.0±4.2 days (mean±SE). The oviposition rate increased very quickly during the first weeks of life, peaking to almost two eggs per day during days 30–44, and gradually dropping in the following weeks. The females laid an average of 67.0±5.96 (mean±SE) eggs through their lives. The hatch rate was 92.6±1.0%; (mean±SE). There were large individual variations in longevity and fecundity parameters, and principal component analysis based on 16 morphological and biological parameters separated the breeding insects into four distinct groups. Almost half of the reproducing beetles were large-sized insects, with high longevity and fecundity traits. Overall, the reproductive potential of the Portuguese population ofM. galloprovincialisseems to be smaller than that described for otherMonochamusvectors of the pine wood nematode both in North America and Japan.

scholarly journals Effectiveness of Bacillus thuringiensis Berliner var. kurstaki on the grape berry moth Lobesia botrana Den. and Shiff. (Lepidoptera, Tortricidae) under field and laboratory conditions in Crete

2017 ◽  
Vol 4 ◽  
pp. 31 ◽  
Author(s):  
Ν.Ε. Roditakis
Keyword(s):  
Bacillus Thuringiensis ◽  
Lobesia Botrana ◽  
Grape Berry ◽  
Egg Laying ◽  
Field Conditions ◽  
Egg Hatching ◽  
Laboratory Conditions ◽  
And Control ◽  
Trap Catches ◽  
Field And Laboratory Conditions

The effectiveness of Bacillus thuringiensis Berliner var. kurstaki (Dipel Abbot Lab.) against the grape berry moth (Lobesia botrana Den. & Shiff.) was evaluated under laboratory and field conditions. Under laboratory conditions (24±0.5°C, 55±5% r.h., 2,000 Lux, and 16 hours light daily) B. thuringiensis was compared to triflumuron and methomyl on egg hatching and shallow entries per grape berry. B. thuringiensis had no effect on egg hatching while methomyl and triflumuron had ovicidal action. Shallow entries per grape berry were 0.9-1, 0, 0 and 4.55 for B. thuringiensis, triflumuron, methomyl and control, respectively. Under field conditions, using as criteria for the timing of sprays: a. pheromone and food trap catches and b. visual counting of egg laying and a threshold of 20-30 eggs/100 grapes, two applications of B. thuringiensis at Kastelli Pediados in 1981 and four at Peza in 1982 were made both at the 2nd and 3rd flights. Its effectiveness was 96-100% and 92% at Kastelli Pediados and 73-75% at Peza.

scholarly journals Fertility and timing of the embryonic development of Cameraria ohridella Deschka & Dimic, 1986 (Lepidoptera: Gracillariidae) in laboratory conditions at different temperatures

2019 ◽  
pp. 27-28
Author(s):  
M. Bashchenko ◽  
V. Chayka
Keyword(s):  
Survival Rate ◽  
Environmental Factors ◽  
Embryonic Development ◽  
Experimental Studies ◽  
Biological Data ◽  
The State ◽  
Egg Laying ◽  
Effect Of Temperature ◽  
Moth Population ◽  
Laboratory Conditions

Goal. The effect of temperature on fertility and the timing of the embryonic development of the chestnut moth under laboratory conditions was studied. Methods. Laboratory. Selected pupae of the chestnut moth were placed in thermostats (at a temperature of 15, 20, 25, 30). Then, 5 pairs of pupae of different sexes were placed in glass cages in which the paper was pre-inserted, as a substrate for egg laying. Experiments were performed in four versions — 10 replications each. Cages with each variant were placed in polythermostats at temperatures of 15, 20, 25 and 30°С. After the butterflies flew out, the paper was taken out of the cage regularly (every two days) and under a microscope of MBS-9 (eyepiece 0.6 magnification 2), the number of laid eggs and caterpillars that hatch were counted. The results were processed statistically by standard methods for biological data. Results. It was determined that at a temperature of 15°C 50% of caterpillars were released from 40 eggs. At 25°C, out of 187 laid chestnut moth eggs, 65% of caterpillars were released. At 20°C, butterflies laid aside 83 instances. 54% of the caterpillars were released. At 30°C, the female chestnut moth was set aside on 17 instances eggs whose embryonic development has not occurred. It was revealed that at 15 and 30°С females are the least active, thus, optimal temperatures for keeping the chestnut moth in laboratory conditions are in the range of 20—25°С. Under these conditions, butterflies mate and lay eggs, the survival rate of which reaches 54—65%. The technique makes it possible in the laboratory to investigate the influence of environmental factors on the state of the chestnut moth population. Findings. It was determined that in laboratory conditions the optimum temperature for the deposition of chestnut moth eggs is 25°C. Butterflies favor the green substrate. Embryonic development lasts 6.1 ± 0.12 days, the survival rate of the populations is 25%. The method of obtaining chestnut moth eggs allows for in-depth experimental studies of the influence of environmental factors on the state of the chestnut moth population.

scholarly journals Effect of a Physical Barrier on Adult Emergence and Egg Survival Associated with the Fungus Gnat, Bradysia sp. nr. coprophila (Diptera: Sciaridae), under Laboratory Conditions

HortScience ◽  
2014 ◽  
Vol 49 (7) ◽  
pp. 905-910 ◽  
Author(s):  
Amy L. Raudenbush ◽  
Raymond A. Cloyd ◽  
Erik R. Echegaray
Keyword(s):  
Biological Control Agent ◽  
Adult Emergence ◽  
Control Agent ◽  
Egg Laying ◽  
Egg Survival ◽  
Recycled Glass ◽  
Laboratory Conditions ◽  
Rove Beetle ◽  
Fungus Gnat ◽  
Series Of Experiments

This study was conducted to assess the direct and indirect effects of Growstones™ aggregates, which are made from recycled glass, on fungus gnat, Bradysia sp. nr. coprophila (Diptera: Sciaridae), adult emergence, female egg-laying capacity, and egg survival. A series of experiments were performed under laboratory conditions to evaluate the effect of different sizes (2.0 to 10.0 mm) of Growstones™ aggregates, layer thicknesses (0.63 to 3.18 cm), and the use of the biological control agent, the rove beetle, Dalotia coriaria, along with different thicknesses (1.27 and 3.18 cm) of small Growstones™ aggregates on fungus gnat adult emergence. For each experiment, Growstones™ aggregates were applied to the surface of the growing medium in 473-mL polypropylene deli containers. This study demonstrated that the thickest (3.18 cm) layer of small (2.0 mm) Growstones™ aggregates significantly reduced or delayed the emergence of fungus gnat adults. In addition, the thickest layer of small Growstones™ aggregates may have indirectly affected egg survival. However, the use of Growstones™ along with rove beetle adults did not significantly reduce fungus gnat adult emergence.

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