scholarly journals Effects of copper nanoparticles on the morphological and physiological changes in chicken

2019 ◽  
Vol 41 (2) ◽  
Author(s):  
Nguyen Thi Xuan ◽  
Phi Thi Thu Trang ◽  
Le Thi Thu Hien
Keyword(s):  
Copper Nanoparticles ◽  
Serum Levels ◽  
Feed Additive ◽  
Control Group ◽  
Cu Content ◽  
Wide Range ◽  
Body Weights ◽  
Liver And Kidney ◽  
Kidney Functions ◽  
Poultry Farming

Copper nanoparticles (nCu) have a wide range of applications in many different fields of life. In poultry farming, nCu is well-known as a feed additive to stimulate the development, a good antimicrobial agent and a suppressor of diarrhea. The aim of this study is to perform the analysis of biosafety of nCu. In this study, chickens were treated with drinking water containing 2 mg/L or 1000 mg/L nCu. After 5 weeks of treatment, the survival rate, dry body weight, Cu content in serum and liver tissues of chickens were examined. Liver and kidney functions were also determined by measuring serum levels of ALT, AST, and creatinine. As a result, chickens treated with drinking containing 2 mg/L nCu had no changes in liver and kidney functions as well as their body weights. However, treatment of chickens with 1000 mg/L nCu caused accumulationof Cu in the serum and liver and increase of the serum levels of ALT and AST compared with control group. Apparently they sick and did not develop properly. In conclusion, nCu concentration at 2 mg/L may be recommended for the agricultural, feed and medicine formulations to meet biological safety.ALT, AST, creatinine, nCu, chicken.

scholarly journals Evaluation of Some Biochemical Parameters and Hormones In Patients with Acute Myeloid Leukemia in Iraq

2021 ◽  
pp. 1460-1466
Author(s):  
Hind Mahmood Jumaah ◽  
Jabbar H. Yenzeel ◽  
Mohammad G. Mehdi
Keyword(s):  
Acute Myeloid Leukemia ◽  
Liver Function ◽  
Kidney Function ◽  
Myeloid Leukemia ◽  
Biochemical Parameters ◽  
Serum Levels ◽  
Newly Diagnosed ◽  
Liver And Kidney ◽  
Kidney Functions ◽  
Acute Myeloid

 The effect of myeloid leukemia,  especially cute myeloid leukemia (AML), has been widely noticed on the parameters of liver and kidney functions and the levels of certain hormones. This study aimed to evaluate a number of biochemical parameters of liver and kidney functions and hormones in Iraqi subjects with newly diagnosed acute myeloid leukemia. Eighty newly diagnosed AML adult patients (40 males and 40 females) and forty healthy individuals (20 males and 20 females) with an age range of 16-75 years were involved in this study during their attendance at the Hematology Department of Baghdad Teaching Hospital/ Medical city in Baghdad province from March 2019 to February 2020. Blood samples were collected from all subjects for the determination of serum levels of the parameters of liver function parameters., kidney function , lactate dehydrogenase (LDH), and Erythropoietin (EPO). The results showed that the serum levels of liver function parameters (alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) had highly significant increases (p< 0.01)  in AML patients (85.87±2.49 , 53.93±1.76, 150.87±7.04 U/L, respectively) as compared  to the control (30.58 ±2.04, 22.89 ±0.97, 75.51 ±2.12 U/L, respectively ). Also, the level of kidney function parameters (blood urea, creatinine and uric acid) showed highly significant increases (p< 0.01) in AML patients (58.82 ±1.49, 1.831 ±0.05, 8.34 ±0.15 mg/dl, respectively) as compared to the control (31.10 ±1.03, 0.850 ±0.02, 4.81 ±0.14 mg/dl, respectively). In addition, the level of LDH showed a highly significant increase (p< 0.01) in the patients with AML (657.72 ±80.76 U/L) as compared to the control (166.05 ±6.15 U/L). Moreover, the level of EPO showed a highly significant increase (p<0.01) in the patients with AML (11763.80 ±329.46 pg/ml ) as compared  to the control (316.94 ±34.42 pg/ml).

scholarly journals Modulatory Effect of Dietary Copper Nanoparticles and Vitamin C Supplementations on Growth Performance, Hematological and Immune Parameters, Oxidative Status, Histology, and Disease Resistance Against Yersinia Ruckeri in Rainbow Trout (Oncorhynchus Mykiss)

2021 ◽  
Author(s):  
Mojtaba Delavari ◽  
Ahmad Gharaei ◽  
Javad Mirdar Harijani ◽  
Aida Davari ◽  
Abolhasan Rastiannasab
Keyword(s):  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Growth Performance ◽  
Copper Nanoparticles ◽  
Control Diet ◽  
Feed Additive ◽  
Control Group ◽  
Yersinia Ruckeri ◽  
Dietary Copper

Abstract The present study aimed to evaluate the effects of dietary copper nanoparticles (Cu-NPs) and vitamin C (VC) supplementations on rainbow trout (Oncorhynchus mykiss) juveniles. Six trial diets were supplemented with Cu-NPs and VC including 0/0 (T1, control diet), 0/250 (T2), 0/500 (T3), 2/250 (T4), 2/500 (T5), and 2/0 (T6) mg Cu-NPs/VC per kg diet. After the feeding trial for 60 days, the fish were challenged with Yersinia ruckeri and the survival rate was calculated for 15 days. Based on the data analysis, weight gain (WG), feed conversion ratio (FCR), specific growth rate (SGR), protein efficiency ratio (PER), lysozyme, alternative complement activity (ACH50), catalase (CAT), glutathione peroxidase (GPX), hematocrit (Hct) and mean corpuscular volume (MCV) were significantly (p < 0.05) affected by the Cu-NPs factor. Meanwhile, VC was a significant factor for hemoglobin (Hb) and superoxide dismutase (SOD) (p < 0.05). The results showed that the Cu-NPs and/or VC-supplemented diets improved WG, FCR, SGR, PER, lysozyme, ACH50, SOD, CAT, GPX, Hb, Hct, and MCV when compared with the control group (p < 0.05). The expressions of TNF-α, IL-1ß, IL-10, SOD, CAT, and GPX genes were significantly (p < 0.05) decreased in the fish fed on T3, T4, and T5 diets versus the control. In addition, the dietary Cu-NPs and VC supplementations significantly enhanced resistance against pathogens and led to the control of infection in rainbow trout. In conclusion, Cu-NPs and VC administered as feed additive at 2/250–500 mg/kg elevated the growth performance, antioxidant capacity, and health of rainbow trout.

scholarly journals COMPARATIVE STUDY OF PATHOGENICITY BETWEEN ASPERGILLUS FUMIGATUS AND PENICILLIUM CHRYSOGENUM IN MICE

2020 ◽  
Vol 51 (Special) ◽  
Author(s):  
S. N. Yassein
Keyword(s):  
Aspergillus Fumigatus ◽  
Penicillium Chrysogenum ◽  
Control Group ◽  
Histopathological Changes ◽  
Active Components ◽  
Internal Organs ◽  
Opportunistic Fungi ◽  
Liver And Kidney ◽  
Kidney Functions ◽  
Intraperitoneal Inoculation

This study was conducted to compare between two types of opportunistic fungi (Aspergillus fumigatus and Penicillium chrysogenum) in concerning their pathogenicity after intraperitoneal inoculation of mice. A total of twenty four male albino mice were used in this study which divided equally into 3 groups, The first and second groups were inoculated with 0.2ml of 1x 107spores/ml of A. fumigatus and P. chrysogenum  intraperitonially respectively, while the third group was inoculated with normal saline which served as control group. All animals were monitored for 2 weeks after infection. The blood samples were collected by heart puncture after 18 days post infection to isolate of serum that used for biochemical analysis of liver and kidney functions. After that, all animals were sacrificed. Some internal organs of infected groups (liver, kidney, intestine, heart, spleen and lung) were taken to study the histopathological changes. It was found that there was severe histopathological changes in studied organs of infected mice particularly liver, kidney, spleen and intestine which corresponding with significant variation (p<0.01) in enzyme activities of liver and kidney like (Alanine Aminotransferase (ALT), Urea and Creatinine). Also, It was found that P. chrysogenum had more impact on these enzymes (15.65 ± 0.78, 135.23 ± 8.75 and 0.928 ± 0.02 respectively) than A. fumigatus (21.70 ± 1.04, 57.91 ± 5.99 and 0.587 ± 0.03 respectively). Therefore, the present study indicated that fungi present in the environment can induce severe inflammation reach to tissue damage in most vital internal organs So, further studies should be performed to determine the specific virulence factors and active components, which are responsible for pathogenesis of A. fumigatus and P. chrysogenum in spite of the fact that P. chrysogenum can produce antibiotic.

scholarly journals Chrysin protects mice liver and kidney from methandienone-induced oxi-dative ‎stress, inflammation a multi-biomarker approach

2019 ◽  
Vol 10 (2) ◽  
pp. 1081-1088
Author(s):  
Marwah Y. Falih ◽  
Abbas A. Mohammed ◽  
Ghassan M. Sulaiman
Keyword(s):  
Combined Treatment ◽  
Serum Levels ◽  
Hepatic Function ◽  
Oxidation Activity ◽  
Biochemical Effects ◽  
Liver And Kidney ◽  
Anti Oxidant ◽  
Mice Liver ◽  
Kidney Functions ◽  
Renal Function Tests

Methandienone is a medication that has wide uses by competitors and young people in ‎gyms ‎for fitness. Chrysin (CR) is a characteristic natural flavonoid that has to incorporate ‎being a ‎cancer prevention agent, anti-inflammatory and anti-oxidant agent. Mus Musculus ‎mice ‎were therefore used in the current work to test the effects of methandienone on ‎vital ‎physiological functions as expressed by the liver, renal function tests and oxidation ‎activity. ‎The reverse effects of chrysin were also tested for the treatment of methandienone in ‎order ‎to determine the possibility of lowering levels of side effects. The results showed ‎clear ‎changes in biochemical effects on liver and kidney functions as a result of treatment ‎with ‎methandienone were also evident; The treated mice showed a significant elevation in ‎the ‎concentrations of serum levels of the hepatic function enzymes (ALP, AST, ALT) ‎and ‎kidney functions parameters (urea and creatinine).‎‏ ‏Combined treatment of mice ‎with ‎‎(methandienone + 25 mg/kg chrysin) caused a significant reduction in urea level ‎and ‎activities of ALP, ALT and AST (p<0.05) as compared to treatment with ‎methandienone ‎alone. Treatment with (methandienone + 50 mg/kg chrysin) also resulted in ‎a significant ‎decrease in the renal level and ALP and AST activities as compared to methandienone- ‎treated ‎mice. The present study concludes that methandienone causes damages to the liver ‎and the ‎kidney, as indicated by elevated levels of their functional enzymes. This effect ‎indicates ‎it increases oxidative stress in these organs, possibly due to elevated production of ‎free ‎radicals.

scholarly journals Effect of the Brown Coal-Based Feed Additive on Poultry Productivity Indicators

2021 ◽  
Author(s):  
Boris Khorin ◽  
Alexandra Danilova ◽  
Natalia Labutina ◽  
Denis Yurin ◽  
Natalia Yurina
Keyword(s):  
Brown Coal ◽  
Laying Hens ◽  
Laying Hen ◽  
Feed Additives ◽  
Egg Laying ◽  
Feed Additive ◽  
Control Group ◽  
Wide Range ◽  
Productivity Indicators ◽  
The Cost

This study examined the effect of feed additives based on brown coal on the productivity indicators of laying hens. The Relict A® organo-mineral feed additive for livestock and poultry is a multi-component, gummatized product with a wide range of biological activity, accelerating the growth and development of the animal. Relict A® is an environmentally friendly supplement that does not contain hormones or synthetic compounds. The control group was fed complete feed without additives. The second, third and fourth groups were fed feed containing 0.04%, 0.06% and 0.10% of the studied additives, respectively. The amount of feed for each group of laying hens was strictly normalized in accordance with their age, according to the recommendations for this cross. The use of the Relict A® feed additive in the laying hens’ rations led to an increase in the average number of eggs per laying hen, a decrease in the cost of feed for producing 1 dozen eggs, and a reduction in the time when the poultry reached the peak egg laying intensity. Feeding the additive to laying hens reduced the cost of producing 1 dozen eggs in the experimental groups, and also increased the profitability of chicken eggs in comparison with the control. The best dose was to use 0.06% of Relict A® by weight of the feed. A 26.6% increase in laying hen egg production was found. Keywords: feed additives, laying hens, egg productivity, brown coal

PRODUCTION OF FEED ADDITIVES FOR ANIMAL HUSBANDRY FROM BROWN COALS ON A MODULAR PRODUCTION LINE (ON THE EXAMPLE OF LLC “BOGRADSKIY GOK”)

2020 ◽  
Vol 4 (41) ◽  
pp. 137-143
Author(s):  
ELENA BEZRUK ◽  
◽  
ANDREY SHCHAPOV ◽  
IGOR RUCH’EV ◽  
KONSTANTIN SOROKIN ◽  
...  
Keyword(s):  
Brown Coal ◽  
Production Line ◽  
Feed Additives ◽  
Research Purpose ◽  
Feed Additive ◽  
Control Group ◽  
Wide Range ◽  
Modular Production ◽  
The Republic ◽  
Meat Productivity

The article presents the composition of known feed additives. The combination of components used in the feed additive can solve the problem of vitamin and energy deficiency, normalize metabolism, remove toxic products accumulated in the gastrointestinal tract, increase the resistance of young animals and adult animals, and avoid losses of dairy and meat productivity. Common disadvantages of feed additives are their multicomponent composition, high labor intensity and duration of production. (Research purpose) The research purpose is in developing the technologies for the production of feed additives with a wide range of biological activity based on the processing of oxidized brown coal on a modular production line. (Materials and methods) The article presents the study of the efficiency of a modular processing line to provide flexibility in the technology of processing brown coal to obtain feed additives. The article presents the practical results of checking the quality of the obtained feed additive on the parameters and operating modes of individual units and aggregates. (Results and discussion) Authors developed a technology for the production of feed additives for cattle. The proposed feed additive was tested in Andreevskoe LLC of the Republic of Khakassia on a dairy herd of cattle, where two groups of animals of 20 heads each were formed: a control group and an experimental group. Authors tested the addition at the end of the winter stall period. (Conclusions) The article presents the influence of the “Humates of Khakassia” feed additive on the dairy and meat productivity of cattle on the example of LLC «Andreevskoe». Authors optimized the feeding standards and the composition of feed additives for dairy cattle in the Republic of Khakassia. The article presents developed recommendations for the use of feed additives in the stable period.

Livestock indicators of broiler chickens rearing when use in feeding of sweet chestnut wood extract

2021 ◽  
pp. 3-12
Author(s):  
N. P. Buryakov ◽  
A. S. Zaikina ◽  
M. A. Buryakova ◽  
M. Shaaban ◽  
A. Yu. Zagarin
Keyword(s):  
Broiler Chickens ◽  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additive ◽  
Control Group ◽  
Sweet Chestnut ◽  
Daily Gain ◽  
Experimental Group ◽  
Chestnut Wood ◽  
Poultry Farming

The intensifi cation of modern industrial poultry farming as the most progressive and dynamically developing branch of agriculture aimed at raising productivity and increasing production volumes, inevitably leads to numerous problems related to poultry health. One of the most common ways to solve this problem is to use antibacterial drugs. The results of scientifi c and economic experiment on the use of the phytobiotic additive “Farmatan VSO (Butitan)” in feeding broiler chickens of cross Cobb-500 have been provided in the article. The inclusion in the poultry diet of the phytobiotic “Farmatan VSO (Butitan)” in the amount for compound feed Start 800 g/t, Growth and Finish – 400 g/t leads to increase in the live weight of broilers by 3,0 % compared to the control group. At the same time the average daily gain of live weight in the 4th experimental group was 73 g, which was by 2,8 % more than in the control group. The use of phytobiotic additives in the feeding of broiler chickens contributes to the highest livability of poultry (98,1 %). It has been found that the best indicators for feed expenditures have been noted in the 3rd and 4th experimental groups 1,47 and 1,48 kg, which were 3,3 and 2,6 % lower than in the control group. Thus, the authors have found that the highest live weight, average daily gain, livability and lowest expenditures of feed per unit of production diff ered in broiler chickens of 4th experimental group where in the compound feed antibiotic Flavomycin has been replaced on phytobiotic feed additive “Farmatan VSO (Butican)” in the compound feed Start 800 g/t, Growth and Finish- 400 g/t. Production testing of the use of “Farmatan VSO (Butitan)” has shown the high eff ectiveness of phytobiotics as a mean of replacing the feed antibiotic and improving the livestock indicators of broiler chickens rearing.

Investigation of Effects of Silymarin in 5-Fluorouracil Hepatotoxicity and Nephrotoxicity in Mice

2021 ◽  
Author(s):  
Emin Sengul ◽  
Volkan Gelen ◽  
Serkan Yildirim ◽  
Esra Senturk ◽  
Yusuf Dag ◽  
...  
Keyword(s):  
Dna Damage ◽  
Side Effects ◽  
Total Bilirubin ◽  
Control Group ◽  
High Dose ◽  
Liver And Kidney ◽  
Group 5 ◽  
High Doses ◽  
Kidney Functions ◽  
Liver And Kidney Damage

Abstract Hepatotoxicity and nephrotoxicity are common side effects of 5-Fluorouracil (5-FU). The present study aimed to investigate the effects of Silymarin (SLY) on 5-FU induced hepatotoxicity and nephrotoxicity in mice. In our study, 10 mice in each group were randomly divided into four groups as the control group, 5-FU, SLY50+5-FU, and SLY100+5-FU group. SLY50+5-FU and SLY100+5-FU groups were administered at a dose of 50 and 100 mg/kg for seven days, respectively. 5-FU was administered at a dose of 400 mg/kg intraperitoneally on the fourth day. After the applications, the mice were decapitated under anesthesia. The liver and kidney functions which urea, creatinine, AST, ALT, and total bilirubin levels were analyzed in serum. In liver and renal tissues, MDA and GSH levels, SOD, CAT, and GR activity were determined. Also, histopathological and immunohistochemical changes were examined in liver and kidney sections. Urea, creatinine, ALT, AST, and total bilirubin levels increased 5-FU group according to control and prevented to this increases the especially high dose of SLY. 5-FU also causes histopathological and immunohistochemical changes such as degeneration, necrosis, hyperemia, DNA damage, and IL-6 increase in kidney and liver tissue. High doses of SLY prevented these changes caused by 5-FU. As a result of this study, it was determined that SLY has hepatoprotective and nephroprotective effects on 5-FU-induced liver and kidney damage in mice.

Biodistribution and Toxicity Study of Titanium Dioxide Nanoparticles of Different Sizes after Intravenous Injection in Mice

2014 ◽  
Vol 998-999 ◽  
pp. 196-199
Author(s):  
Jie Fang ◽  
Lu Lu Yuan ◽  
Chen Jie Yao ◽  
Yan Li Wang ◽  
Ming Hong Wu
Keyword(s):  
Titanium Dioxide ◽  
Biochemical Parameters ◽  
Titanium Dioxide Nanoparticles ◽  
Control Group ◽  
Quantitative Distribution ◽  
Blood Biochemical ◽  
Long Time ◽  
Liver And Kidney ◽  
Kidney Functions

Titanium dioxide nanoparticles (TiO2-NPs) are widely used in many fields. The bio-distribution and toxicology of different sizes (25 nm and 50 nm) of TiO2-NPsin vivois evaluated in this paper. The results of quantitative distributionin vivoshow that it is difficult for TiO2-NPs to excretion clearly from tissues and organs. At the endpoint of experiment of different groups, there are still many TiO2-NPs located in the main organs such as spleen, liver and lung after 120 days. Blood biochemical parameters keep unchanged compared with control group. However, pathological results show that lesions in spleen, liver and lung where TiO2-NPs particles are mainly accumulated and retained are getting serious with the time prolonging. The results indicate that TiO2-NPs are low toxic to mice but may have some potential effects on liver and kidney functions after exposure long time. The fundamental importance information gained from such a study will give the guidance on the application of TiO2-NPs.

scholarly journals Biochemical alterations in Horses Infected with Theileria equi

2018 ◽  
Vol 14 (2) ◽  
Author(s):  
T. M. Vidhyalakshmi ◽  
S. K. Raval ◽  
P. V. Parikh ◽  
P. V. Patel
Keyword(s):  
Serum Levels ◽  
Significant Elevation ◽  
Serum Samples ◽  
Theileria Equi ◽  
Healthy Group ◽  
Biochemical Alterations ◽  
Liver And Kidney ◽  
Severity Of The Disease ◽  
Kidney Functions ◽  
G Ratio

Equine theileriosis, an OIE listed disease caused by Theileria equi is an economically important haemoprotozoan disease of horses in tropical and subtropical parts of the world. The aim of the present study was to monitor the biochemical alterations in order to find out the severity of the disease. Standard assay kits were used to study the biochemical parameters of horses positive for theileriosis in comparison with healthy ones. Out of 75 serum samples, 25 samples were collected from horses positive for theileriosis and 50 from healthy horses. The serum levels of GPT (32.00±2.30 U/L), GOT (350.84±27.87 U/L), GGT (20.95±0.92 U/L), LDH (823.74±102.93 U/L), total protein (8.11±0.37 g/dl), globulin (3.84±0.40 g/dl), BUN (46.26±5.27 mg/dl) and creatinine (1.33±0.18 mg/dl) were found to be significantly increased (p less than 0.01) in infected horses compared to the healthy group. Non- significant elevation (p greater than 0.05) in total bilirubin (1.08±0.14 mg/dl), unconjugated bilirubin (0.57±0.11 mg/dl) and glucose (102.57±30.41 mg/dl) as well as non-significant reduction (p>0.05) in conjugated bilirubin (0.42±0.04 mg/dl), albumin (4.26±0.24 g/ dl) and A/G ratio (1.55±0.30) were also observed in infected group. There was no significant alteration (p>0.05) in the level of serum calcium (12.32±0.36 mg/dl) and phosphorus (4.53±0.33 mg/dl), as compared to the healthy group. Significant elevation of GOT, GPT, GGT, BUN and creatinine revealed the involvement of liver and kidney functions in T. equi infected horses.

Sign in / Sign up

Export Citation Format

Share Document