The Effect of Broccoli Extract in Arsenic-Induced Experimental Poisoning on the Hematological, Biochemical, and Electrophoretic Parameters of the Liver and Kidney of Rats

Heavy metals such as arsenic contribute to environmental pollution that can lead to systemic effects in various body organs. Some medicinal plants such as broccoli have been shown to reduce the harmful effects of these heavy metals. The main aim of the present study is to evaluate the effects of broccoli extract on liver and kidney toxicity, considering hematological and biochemical changes. The experimental study was performed in 28 days on 32 male Wistar rats classified into four groups: the control group (C), a group receiving 5 mg/kg oral arsenic (AS), a group receiving 300 mg/kg broccoli (B), and a group receiving arsenic and broccoli combination (AS + B). Finally, blood samples were taken to evaluate the hematological and biochemical parameters of the liver and kidney, as well as serum proteins’ concentration. Liver and kidney tissue were fixed and stained by H&E and used for histopathological diagnosis. The results demonstrated a significant decrease in white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb) in the AS group compared to other groups. However, in the B group, a significant increase in RBC and WBC was observed compared to the AS and C groups ( P < 0.05). Moreover, RBC and WBC levels increased significantly in the AS + B group compared to the AS group ( P = 0.046). However, in the AS group, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine levels increased, while total protein, albumin, and globulin decreased. This can be a result of liver and kidney damage, which was observed in the AS group. Furthermore, the increase in the concentration of albumin and globulin in the AS + B group was higher than that in the AS group. Infiltration of inflammatory cells and necrosis of the liver and kidney tissue in the pathological evaluation of the AS group were significantly higher than other groups. There was an increase in superoxide dismutases (SOD), glutathione peroxidase (GPx), and total antioxidant capacity (TAC); however, a decrease in malondialdehyde (MDA) concentration was seen in the AS + B group compared to the AS group. It seems that broccoli is highly effective at reducing liver and kidney damage and improving the hematological and biochemical factors in arsenic poisoning conditions.

Rutin Protects from Destruction by Interrupting the Pathways Playing a Role in the Possible Damage Mechanism of Sodium Valproate in the Liver and Kidney Tissues of Rats

Background: The present study investigated the effects of rutin (RUT), which has various biological and pharmacological properties, on liver and kidney damage caused by histone deacetylase inhibitor valproic acid (VLP), which is used in the treatment of many psychiatric disorders.Methods and Results: In the study, 50 or 100 mg/kg RUT treatment was administered 30 minutes after 500 mg/kg VLP was given to rats for 14 days. Then, some pathways that may be involved in the damage mechanism of VLP in liver and kidney tissues were investigated using biochemical, RT-PCR and Western blotting techniques. The results show that the levels of MDA induced by VLP in liver and kidney tissues decreased after RUT treatment, and the levels of SOD, CAT, GPx and GSH suppressed by VLP increased after RUT administration. It was observed that ER stress induced by oxidative stress was alleviated by suppressing the expressions of ATF-6, PERK, IRE1 and GRP78 after RUT treatment. It was observed that the expressions of NF-kB, TNF-a, IL-6, JAK2 and STAT3 in the inflammatory pathway increased after VLP administration, while RUT treatment decreased the levels of these markers. It is also among the data obtained that the levels of markers that play a role in the regulation of apoptosis (Bax, Bcl-2, kaspaz-3, pERK, pJNK) or autophagy (Beclin-1, LC3A, LC3B) approach the control group after RUT treatment.Conclusions: Taken together, it was determined that RUT treatment protected against liver and kidney damage by attenuating VLP-induced oxidative stress, ER stress, inflammation, apoptosis and autophagy.

Persistent Purine Metabolic Abnormality Induces the Aggravation of Visceral Inflammation and Intestinal Microbiota Dysbiosis in Magang Goose

Gout is a disease involving abnormal purine metabolism that is widespread in mammals and birds. Goose is especially susceptible for gout in early stage. However, a few studies investigated the ontogenetic pattern of goslings with purine metabolic abnormality. Our studies were conducted to investigate whether persistent purine metabolic abnormality would lead to aggravation of visceral inflammation and intestinal microbiota dysbiosis in goose. A total of 132 1-day-old Magang geese were randomly divided into six replicates and fed a high-calcium and protein meal-based diet from 1 to 28 days. The experiment lasted for 28 days. Liver and kidney damages were observed in 14- and 28-day-old Magang geese, and liver inflammation increased with increasing age. In 28-day-old Magang geese, serum CAT and liver GSH-Px activity were significantly reduced. Furthermore, jejunum intestinal barrier was impaired and the abundance of Bacteroides was significantly reduced at the genus level. Collectively, the high-calcium and high-protein (HCP) meal-based diet caused liver and kidney damage in 28-day-old Magang geese, leading to hyperuricemia and gout symptoms, and the intestinal barrier is impaired and the intestinal flora is disrupted.

Investigation of Effects of Silymarin in 5-Fluorouracil Hepatotoxicity and Nephrotoxicity in Mice

Hepatotoxicity and nephrotoxicity are common side effects of 5-Fluorouracil (5-FU). The present study aimed to investigate the effects of Silymarin (SLY) on 5-FU induced hepatotoxicity and nephrotoxicity in mice. In our study, 10 mice in each group were randomly divided into four groups as the control group, 5-FU, SLY50+5-FU, and SLY100+5-FU group. SLY50+5-FU and SLY100+5-FU groups were administered at a dose of 50 and 100 mg/kg for seven days, respectively. 5-FU was administered at a dose of 400 mg/kg intraperitoneally on the fourth day. After the applications, the mice were decapitated under anesthesia. The liver and kidney functions which urea, creatinine, AST, ALT, and total bilirubin levels were analyzed in serum. In liver and renal tissues, MDA and GSH levels, SOD, CAT, and GR activity were determined. Also, histopathological and immunohistochemical changes were examined in liver and kidney sections. Urea, creatinine, ALT, AST, and total bilirubin levels increased 5-FU group according to control and prevented to this increases the especially high dose of SLY. 5-FU also causes histopathological and immunohistochemical changes such as degeneration, necrosis, hyperemia, DNA damage, and IL-6 increase in kidney and liver tissue. High doses of SLY prevented these changes caused by 5-FU. As a result of this study, it was determined that SLY has hepatoprotective and nephroprotective effects on 5-FU-induced liver and kidney damage in mice.

Comparative Effect of Ginger (Zingiber officinale) Supplement on Hepato-renal Damages Induced by Acetaminophen Toxicity in Wistar Rats

Background: Acetaminophen toxicity is one of the widespread causes of both planned and unplanned health related issues in the world today. It is a familiar cause of liver and kidney damage particularly in adolescents and children. Aims: With the growing focus in the mitigating and therapeutic roles of herbal drugs in toxicity studies, it is perceptible to investigate the comparative, prophylactic and curative effect of the ginger supplement on acetaminophen-induced liver and kidney damage using wistar rats as model. Methodology: Study Design: Twenty (20) adult male wistar rats weighing185 g-220 g was obtained from animal house of the department of Pharmacology University of Port Harcourt were divided into four groups having five rats each. Methodology: Twenty adult wistar rats were divided into four (4) groups of five animals each and treated orally as follows: Group A (normal control) received distilled water (7days), group B received 1000mg/kg acetaminophen (APAP) (2 days). Group C Ginger (GINE+APAP) received ginger supplements 500mg/kg respectively one hour before administration of APAP for 7 days, whereas group D received APAP (2 days) followed by 500mg/kg Ginger for 5 days (APAP+GINE). At the end of the experiment, animals from different groups were anaesthesized, blood samples collected, liver and kidney tissues dissected and subjected to different biochemical, antioxidants, and histopathological test. Statistical: Analysis was done using One Way Anova and Tukey’s Post-hoc Test. Results: APAP caused significant (P<0.05) decrease in creatinine with significant (P<0.01) increase and decrease in liver enzymes and hepatic catalase levels respectively in relation to normal control. Treatment of rats with the ginger supplements attenuated the elevated liver and kidney biochemistry, histopathological alterations by APAP treatment. Conclusively: The extracts demonstrated therapeutic ability and the herbal drugs may be more effective when use prophylactically than curatively. Further research work is needed to validate the finding.

Cardiac Arrests after Eating Raw Grass Carp Gall Bladders: A Case Report

BACKGROUND: Cases of poisoning by eating raw fish gallbladder occur mainly in Asia. These cases have been reported mainly with acute liver and kidney damage. We found that the ingestion of carp gallbladder also caused myocardial damage and lead to frequent cardiac arrest in patients with subclinical long QT interval syndrome. Patient concerns: We report a 57-year-old woman with an unexplained Long QT syndrome history who developed arrhythmia sudden death two times following ingestion of raw fish gall bladder.Fish bile poisoning, toxic hepatitis, acute renal failure, cardiac arrest, postoperative cardiopulmonary resuscitation, long QT syndrome were diagnosed. As in this case, myocardial injury caused by fish bile poisoning further leads to cardiac arrest related to LQTS.The patient underwent continued renal replacement therapy (CRRT) and other supportive treatments to manage symptoms, promote detoxing, protect liver and myocardium in our hospital. Then she completed a cardiac pacemaker placement in local hospital.Her liver enzymes profile returned to normal after 18 days, and kidney injury index returned to normal after more than one month. On follow up after three months, her renal as well as the liver parameters were found normal and she never attack malignant arrhythmia again.Conclusion: Eating fish bile can lead to MODS, not merely to liver and kidney damage. Some of the LQTS patients usually have no clinical symptoms. Clinicians should be aware of changes in low-risk LQTS patients when something special happens to them.

IDENTIFIKASI BAHAN PEWARNA NAPHTHOL YELLOW S (C10H6N2NaO8S+) DALAM SEDIAAN PERONA MATA SECARA KROMATOGRAFI LAPIS TIPIS (KLT)

Naphtol yellow S is a synthetic dye used for coloring batik. In cosmetics, naphtol yellow S is abused for eye shadow coloring to get more attractive colours, even though the use of naphtol yellow S can cause negative effects such as eye and respiratory tract irritation, dangerous if swallowed or inhaled will cause blood to become abnormal and followed liver and kidney damage. This study aims to identify naphtol yellow S in eye shadow. The coloring agent analysis was determined by Thin Layer Chromatography (TLC). Based on the result, there were no naphtol yellow S stains found in the sample. It can be concluded that the eye shadow samples used did not contain naphtol yellow S. Keywords: Naphtol Yellow S, Cosmetics, Eye Shadow, TLC