scholarly journals Immobilizing Alcalase® Enzyme onto Magnetic Nanoparticles

2014 ◽  
Vol 24 (3S1) ◽  
pp. 121-126
Author(s):  
Cao Xuan Huu ◽  
Nguyen Bao Linh ◽  
Nguyen Thi Hoang Yen ◽  
Dang Duc Long
Keyword(s):  
Magnetic Nanoparticles ◽  
Alkaline Protease ◽  
Immobilized Enzyme ◽  
Biological Molecules ◽  
Covalent Attachment ◽  
Covalent Bonds ◽  
External Magnet ◽  
Superparamagnetic Property ◽  
Ft Ir ◽  
Reaction Media

In recent years, magnetic nanoparticles (MNPs) have been applied to numerous biological systems. The nanoparticles are particularly useful in separating biological molecules due to its low price, scalable ability and very little interference. Here, MNPs, which can efficiently separate biocatalysts from reaction media by external magnet, was used to immobilize an alkaline protease (Alcalase®). Covalent attachment of the enzyme to MNPs began with the functionalization of the MNPs' surface with amines (APTES). Then, glutaraldehyde was introduced to link the MNP surface amines with enzyme surface amine residues, typically lysine. Successful covalent bonds were checked by FT-IR. Our results showed the attached enzyme did not affect superparamagnetic property of MNPs, therefore the MNPs-attached enzyme was easily recovered after the reaction. The immobilized enzyme maintained its activities after 10 times of recycle uses.

scholarly journals Chitosan-Collagen Coated Magnetic Nanoparticles for Lipase Immobilization – New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

2016 ◽  
Author(s):  
Marta Ziegler-Borowska ◽  
Dorota Chelminiak-Dudkiewicz ◽  
Tomasz Siódmiak ◽  
Adam Sikora ◽  
Katarzyna Wegrzynowska-Drzymalska ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Immobilized Enzyme ◽  
Candida Rugosa ◽  
Squaric Acid ◽  
Lipase Immobilization ◽  
Polymer Shell ◽  
Tem Analysis ◽  
Coated Nanoparticles ◽  
New Type ◽  
Ft Ir

The synthesis of new collagen, chitosan and chitosan-collagen coated magnetic nanoparticles have been done. Two types of cross-linkers for polymer shell stabilization were used: glutaraldehyde (Gla) as a standard cross-linker and new one – squaric acid (SqA). Structure and morphology of prepared nanoparticles were characterized by ATR-FT IR, XRD and TEM analysis. The immobilization of lipase from Candida rugosa was performed on the nanoparticles surface. The amount of immobilized enzyme was quantified by the Bradford method. All of lipase-biopolymers coated nanoparticles were characterized with good activity recovery. A little hyperactivation of lipase immobilized on nanoparticles with SqA was observed. All of prepared lipase-immobilized nanoparticles were characterized with very good reusability.

scholarly journals Immobilization of Phospholipase D on Silica-Coated Magnetic Nanoparticles for the Synthesis of Functional Phosphatidylserine

Catalysts ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 361 ◽  
Author(s):  
Qingqing Han ◽  
Haiyang Zhang ◽  
Jianan Sun ◽  
Zhen Liu ◽  
Wen-can Huang ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Magnetic Nanoparticles ◽  
Phospholipase D ◽  
Physical Adsorption ◽  
Catalytic Reactions ◽  
Covalent Attachment ◽  
Synthesis Process ◽  
Transmission Electron ◽  
Ft Ir ◽  
Magnetic Nanoparticles Fe3o4

In this study, silica-coated magnetic nanoparticles (Fe3O4/SiO2) were synthesized and applied in the immobilization of phospholipase D (PLDa2) via physical adsorption and covalent attachment. The immobilized PLDa2 was applied in the synthesis of functional phosphatidylserine (PS) through a transphophatidylation reaction. The synthesis process and characterizations of the carriers were examined by scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier-transform infrared spectroscopy (FT-IR). The optimum immobilization conditions were evaluated, and the thermal and pH stability of immobilized and free PLDa2 were measured and compared. The tolerance to high temperature of immobilized PLDa2 increased remarkably by 10°C. Furthermore, the catalytic activity of the immobilized PLDa2 remained at 40% after eight recycles, which revealed that silica-coated magnetic nanoparticles have potential application for immobilization and catalytic reactions in a biphasic system.

Magnetic Nanoparticles Supported Carbene and Amine Based Metal Complexes in Catalysis

2016 ◽  
Vol 42 ◽  
pp. 112-135 ◽  
Author(s):  
Siddappa A. Patil ◽  
Shivaputra A. Patil ◽  
Renukadevi Patil
Keyword(s):  
Catalytic Activity ◽  
Magnetic Nanoparticles ◽  
Metal Complexes ◽  
Review Article ◽  
Organic Reactions ◽  
Research Topics ◽  
External Magnet ◽  
Design And Synthesis ◽  
Catalytic Applications ◽  
Reaction Media

Catalysis is one of the hottest research topics in chemistry. In recent years, metal complexes attracted great interest as catalysts towards various types of organic reactions. However, these catalysts, in most cases, suffer from the deficits during their recovery, recycling and the difficulty in separation of catalysts from the products. Therefore, the design and synthesis of recoverable and recyclable catalyst is very important aspect in catalysis. The aim of this review article is to highlight the speedy growth in the synthesis and catalytic applications of magnetic nanoparticles (Fe3O4, MNPs) supported N-heterocyclic carbene (NHC) and amine based metal complexes in various organic reactions. Furthermore, these catalysts can be easily separated from the reaction media with the external magnet and reused various times without a substantial loss of catalytic activity.

scholarly journals Immobilization of bromelain on cobalt-iron magnetic nanoparticles (CoFe2O4) for casein hydrolysis

2020 ◽  
Vol 49 (1) ◽  
pp. 3-10
Author(s):  
José Mauricio García Colmenares ◽  
Julia Constanza Reyes Cuellar
Keyword(s):  
Catalytic Activity ◽  
Magnetic Nanoparticles ◽  
Immobilized Enzyme ◽  
Covalent Bonds ◽  
Magnetite Nanoparticle ◽  
Casein Hydrolysis ◽  
Transmission Electron ◽  
Cobalt Iron ◽  
Menten Constant ◽  
Thermal Stability Of

By means of recycling an enzyme, bromelain was used in casein hydrolysis facilitated by a nanobiocatalyst consisting of bromelain, CoFe2O4 magnetic nanoparticles, chitosan, and glutaraldehyde. Bromelain was immobilized on the chitosan cobalt-magnetite nanoparticle surface via covalent bonds to form the nanobiocatalyst. Immobilized bromelain showed 77% immobilization binding, retaining 85 ± 2% of the initial catalytic activity. Nanoparticles and immobilized bromelain were characterized using UV-Vis and IR spectroscopies, X-ray, square wave voltammetry (SWV), cyclic voltammetry (CV), vibrating-sample magnetization (VSM), and transmission electron microscope (TEM). The Michaelis-Menten constant (KM) and VMAX of the free and immobilized enzyme were calculated: KM = 2.1 ± 0.18 mM and 1.8 mM, respectively and VMAX = 6.08 x 10-2 ± 2.1 x 10-2 U/min and 6.46 ± 0.91 U/min, respectively. The thermal stability of the free enzyme was higher than the immobilized enzyme: 95-98% and 83-87%, respectively. An optimum pH of 6 and a temperature of 20 °C were determined in both cases. Immobilized bromelain maintained 50% of the initial catalytic activity after the fifth use. The immobilized bromelain proved to be effective and reusable for casein hydrolysis. As novel contribution the characterization by VOC and CV was carried out.

scholarly journals Post-process optimization of 3D printed poly(lactic-co-glycolic acid) dental implant scaffold for enhanced structure and mechanical properties: effects of sonication duration and power

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
R. N. V. C. Virinthorn ◽  
M. Chandrasekaran ◽  
K. Wang ◽  
K. L. Goh
Keyword(s):  
Structural Damage ◽  
Glycolic Acid ◽  
Solvent Mixture ◽  
Ultrasonic Waves ◽  
Poly Vinyl Alcohol ◽  
Covalent Bonds ◽  
Process Stage ◽  
Post Process ◽  
3D Printed ◽  
Ft Ir

AbstractWe described a technique of a post-process stage to partially remove the poly(vinyl alcohol) (PVA) binder in Poly(lactic-co-glycolic acid) (PLGA) dental scaffolds. The scaffolds were exposed to ultrasonic waves while immersed in an ethanol/acetone solvent mixture that possessed both polar and nonpolar properties. A factorial experiment was conducted in which the scaffolds were treated to three levels of sonication power (pW): 0, 20% (22 W) and 40% (44 W), and soaking duration (t): 5, 15, and 30 min. The treated scaffolds were characterized by FT-IR, optical microscopy, and mechanical (compressive) testing. FT-IR revealed that the amount of PVA decreased with increasing pW and t. Two-way ANOVA revealed that increasing pW and t, respectively, resulted in increasing scaffold surface area to volume (SVR). Sonication and solvent caused structural damage (i.e., unevenness) on the scaffold surface, but the damage was minimal at 20% pW and 30 min. The optimal values of pW and t resulting in enhanced fracture strength, strain and toughness were 20% and 30 min, respectively, which corroborated the findings of minimal structural damage. However, sonication had no significant effects on the scaffold stiffness. Mechanistic analysis of the effects of sonication predicted that the ultrasonic energy absorbed by the scaffold was sufficient to disrupt the van Der Waals bonds between the PVA and PLGA but not high enough to disrupt the covalent bonds within the PLGA. This technique is promising as it can partially remove the PVA from the scaffold, and mitigate problematic issues down the line, such as thermal degradation during sterilization, and undue delay/variability in biodegradation.

Magnetic Nanoparticles Derivatized with Dual Functional Groups for Environmental Applications

2010 ◽  
Vol 148-149 ◽  
pp. 949-952
Author(s):  
Hai Bo He ◽  
Qing Zhong Guo
Keyword(s):  
Magnetic Nanoparticles ◽  
Environmental Remediation ◽  
Infrared Spectrometry ◽  
X Ray Diffraction ◽  
Water Contact ◽  
Dual Functional ◽  
New Material ◽  
Ft Ir ◽  
Contact Angle Analysis ◽  
Effective Sorbent

In this study, the magnetic nanoparticles derivatized with dual functional moieties of dodecyl and mercapto were prepared, which characterized by X-ray diffraction (XRD), scanning electron microscope(SEM), surface area and pore size determination, fourier transform infrared spectrometry (FT-IR) and water contact angle analysis. The new material was proved to be an effective sorbent for environmental remediation.

scholarly journals Application of Magnetic Nanoparticles with Structure of Core-shell, as Bactericidal

2018 ◽  
Vol 2 (1) ◽  
pp. 01-04
Author(s):  
Mansour Binandeh
Keyword(s):  
Magnetic Nanoparticles ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Correlation Method ◽  
Core Shell ◽  
Ft Ir ◽  
Chemical Correlation ◽  
Ir Analysis ◽  
The Magnetic Field

Initially, magnetic nanoparticles (MNP) Fe3O4 are synthesized by a chemical correlation method and its core / shell structure is detected using SEM, FT-IR analysis. The purpose of this production was to use the nanoparticle performance level in the absorption of antibiotics, namely, ampicillin (amp). Absorption sampling was analyzed by UV-Vis spectrophotometer and the results indicate that the absorbance of the ampere increases to 85%. The bond between these two is electrostatic bonding, which was confirmed by EDX analysis. Ultimately, this compound was used for the antibacterial process. In this case, the MNP-amp compound was added in a natural amount of 20 μl a bacterial culture pattern overnight (In-vitro). The results showed that 95% of the bacteria were killed (confirmation of antibacterial properties of MNP). Therefore, it can be transmitted intentionally by controlling the magnetic field into living cells for the destruction of pathogenic bacteria.

Magnetic Nanoparticles Supported Acidic Ionic Liquid as a Highly Active and Reusable Catalyst for Esterification

2016 ◽  
Vol 852 ◽  
pp. 485-488 ◽  
Author(s):  
Qiang Zhang ◽  
Xin Zhao ◽  
Xue Hua Zhu ◽  
Ji Hang Li
Keyword(s):  
Ionic Liquid ◽  
Catalytic Activity ◽  
Magnetic Nanoparticles ◽  
Catalytic Performance ◽  
Significant Loss ◽  
Reusable Catalyst ◽  
Hydrogen Sulfate ◽  
External Magnet ◽  
Acidic Ionic Liquid ◽  
Highly Active

A magnetic nanoparticles supported dual acidic ionic liquid catalyst was prepared via anchoring 3-sulfobutyl-1-(3-propyltriethoxysilane) imidazolium hydrogen sulfate onto the surface of silica-coated Fe3O4 nanoparticles. And this novel supported acidic ionic liquid catalyst showed good catalytic performance in esterification. More importantly, the catalyst could be easily recovered by an external magnet and reused six times without significant loss of catalytic activity.

scholarly journals Pharmaceutical Applications of Iron-Oxide Magnetic Nanoparticles

2019 ◽  
Vol 5 (3) ◽  
pp. 50 ◽  
Author(s):  
Marcos Luciano Bruschi ◽  
Lucas de Alcântara Sica de Toledo
Keyword(s):  
Drug Delivery ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Covalent Attachment ◽  
Conceptual Information ◽  
Target Drug Delivery ◽  
Advantages And Disadvantages ◽  
Target Drug ◽  
Pharmaceutical Applications ◽  
Iron Oxide Magnetic Nanoparticles

Advances of nanotechnology led to the development of nanoparticulate systems with many advantages due to their unique physicochemical properties. The use of iron-oxide magnetic nanoparticles (IOMNPs) in pharmaceutical areas increased in the last few decades. This article reviews the conceptual information about iron oxides, magnetic nanoparticles, methods of IOMNP synthesis, properties useful for pharmaceutical applications, advantages and disadvantages, strategies for nanoparticle assemblies, and uses in the production of drug delivery, hyperthermia, theranostics, photodynamic therapy, and as an antimicrobial. The encapsulation, coating, or dispersion of IOMNPs with biocompatible material(s) can avoid the aggregation, biodegradation, and alterations from the original state and also enable entrapping the bioactive agent on the particle via adsorption or covalent attachment. IOMNPs show great potential for target drug delivery, improving the therapy as a consequence of a higher drug effect using lower concentrations, thus reducing side effects and toxicity. Different methodologies allow IOMNP synthesis, resulting in different structures, sizes, dispersions, and surface modifications. These advantages support their utilization in pharmaceutical applications, and getting suitable drug release control on the target tissues could be beneficial in several clinical situations, such as infections, inflammations, and cancer. However, more toxicological clinical investigations about IOMNPs are necessary.

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