scholarly journals IMPACT OF AMPK ACTIVATOR METFORMIN ON SPERM QUALITY

2018 ◽  
Vol 15 (4) ◽  
pp. 605-615
Author(s):  
Nguyen Thi Mong Diep ◽  
Elisabeth Blesbois
Keyword(s):  
Sperm Motility ◽  
Acrosome Reaction ◽  
Positive Impact ◽  
Sperm Quality ◽  
Fluorescent Intensity ◽  
Freeze Thaw ◽  
The Media ◽  
Cryopreserved Sperm ◽  
Amp Activated Protein Kinase

Semen cryopreservation allows crucial management of animal genetic diversity. However, the freeze-thaw process causes biochemical and physical alterations, impairing sperm energy-dependent functions. Currently, many chemicals are added to the media to enhance frozen-thawed sperm quality during artificial insemination. The aims of this study was to determine the effects of Metformin (Metf) on fresh chicken sperm motility and ability to perform acrosome reaction, and to evaluate Metf’s effects on the functions of cryopreserved sperm. Chicken semen was diluted and incubated at 35°C in media supplemented with or without different doses of 5’-AMP-Activated Protein Kinase (AMPK) activator, Metf (0,5 to 5 mM). We then looked for the concentration improving the most sperm quality to use it in the cryopreservation media used for chicken sperm. Our results show that 1 mM Metf is the concentration giving the best results regarding sperm quality. AMPKα phosphorylation, viability, acrosome reaction ability (AR), and various motility parameters, were negatively affected by the freeze-thaw process, and that Metf partially restored them. Sperm quality improved (mean increased by 23% for motility, by 10% for viability) as well as AMPKα phosphorylation (mean increased by 30%). Moreover, fluorescent intensity levels of phospho-AMPK were also stronger with Metf than in the control. These results show that the presence of Metf in fresh semen has a positive impact on the quality of sperm and helps reducing the gradual decline in sperm motility caused by cryopreservation by partially restoring several essential sperm functions, and thus leads to a better overall quality of cryopreserved sperm.

scholarly journals Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

2015 ◽  
Vol 75 (3) ◽  
pp. 662-669 ◽  
Author(s):  
EG Sanches ◽  
IR Oliveira ◽  
PCS Serralheiro ◽  
VR Cerqueira
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Equilibration Time ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ph Values ◽  
Cryopreserved Sperm ◽  
Time Density ◽  
Motility Rate

AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

Effect on sperm quality of different cryoprotectants in sperm of Chinchilla lanigera

Zygote ◽  
2012 ◽  
Vol 21 (4) ◽  
pp. 351-357 ◽  
Author(s):  
M.C. Gramajo-Bühler ◽  
F. Pucci-Alcaide ◽  
G. Sánchez-Toranzo
Keyword(s):  
Acrosome Reaction ◽  
Sperm Quality ◽  
Genetic Material ◽  
Membrane Integrity ◽  
Epididymal Sperm ◽  
Assisted Reproduction Techniques ◽  
Thawing Process ◽  
The Media ◽  
Freezing Storage

SummaryChinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen–thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material.

scholarly journals Effect of Centrifugation on Motility, Sperm Capacitation and Acrosome Reaction in Soy Bean and Avocado Seed Milk Extenders of Cryopreserved Goat Spermatozoa

2017 ◽  
Vol 50 (1) ◽  
pp. 13-18
Author(s):  
James Ola Daramola
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Acrosome Reaction ◽  
Sperm Quality ◽  
West African ◽  
West African Dwarf ◽  
Artificial Vagina ◽  
Avocado Seed

Abstract Removal of seminal plasma by centrifugation (0 centrifugation, 1 centrifugation, 2 centrifugations, 3 centrifugations) and preservation in two different tris-extenders viz., avocado seed milk (ASM) and soy bean milk (SBM) based extenders were studied for their ability to support motility, in vitro capacitation and acrosome reaction of spermatozoa obtained from West African Dwarf (WAD) goat bucks during cryopreservation. Semen samples collected with the aid of artificial vagina were centrifuged for one, two and three times. The centrifuged samples were diluted with the two tris-extenders each containing 20 mL of avocado seed milk and soybean milk and cryopreserved for 30 days. The results showed higher (P < 0.05) sperm motility (P < 0.05) with increased centrifugation times. Spermatozoa that were centrifuged had higher (P < 0.05) percentage of acrosome reaction and capacitation with increased centrifugation times compared to the control. Optimal improvement in these parameters was obtained with increased centrifugation times. The findings revealed that removal of seminal plasma by centrifugation improved sperm quality of WAD goat bucks during cryopreservation and optimum improvement was achieved consistently with 3 centrifugations.

scholarly journals Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

2017 ◽  
Vol 38 (5) ◽  
pp. 3069 ◽  
Author(s):  
Ana Carolina Rodrigues ◽  
Camila Montanari Ruiz ◽  
Carla Daniela Dan De Nardo ◽  
Gabriele Barros Mothé ◽  
Fabiano Martinez Rossi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Reproductive Efficiency ◽  
Omega 3 ◽  
Oral Supplementation ◽  
Animal Reproduction ◽  
Fresh Semen

For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1st-5th week) or pre-supplementation; M2 (6th-9th week) and M3 (10th-13th week) or during supplementation; and M4 (14th-17th week) or post-supplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen.

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

Sperm viability and morphology of two genetically diverse Merino lines

2000 ◽  
Vol 12 (6) ◽  
pp. 337 ◽  
Author(s):  
H. Lambrechts ◽  
F. E. van Niekerk ◽  
S. W. P. Cloete ◽  
W. A. Coetzer ◽  
G. van der Horst
Keyword(s):  
Adverse Effect ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Parameters ◽  
Sperm Viability ◽  
Base Population ◽  
Epididymal Sperm ◽  
High Line ◽  
Computer Aided

Microscopically evaluated sperm parameters, as well as computer-aided sperm motility analysis (CASMA), were used to assess sperm quality and the effect of cryopreservation on ram semen obtained from two genetically diverse Merino lines. These lines were divergently selected on maternal ranking values for multiple rearing ability from the same base population since 1986. Replacements in the high (+) line were preferentially the progeny of ewes rearing >1 lamb per joining. Progeny of ewes rearing <1 lamb per joining was preferred as replacements in the low (–) line. Sperm quality, as assessed by percentages of live, abnormal and acrosome-intact spermatozoa as well as by motility, was independent (P≤0.20) of line, time of sampling and their interaction in ejaculated samples obtained from the eight rams used as sires in 1995. Sperm quality of frozen–thawed samples was adversely affected (P≤0.01) by cryopreservation and thawing at 35˚C for 30 s relative to fresh ejaculated samples. No consistent differences between lines were found in epididymal sperm samples obtained from 12 slaughtered rams (6 from each line). The adverse effect (P≤0.05) of cryopreservation and thawing at 35˚C for 30 s on sperm viability and motility was also demonstrated for these samples.

scholarly journals PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating)

2016 ◽  
Vol 10 (2) ◽  
pp. 98-102
Author(s):  
Trinil Susilawati ◽  
Feri Eka Wahyudi ◽  
Inna Anggraeni ◽  
Nurul Isnaini ◽  
Muhammad Nur Ihsan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Serum ◽  
Sperm Motility ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Egg White ◽  
Cattle Blood

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.

scholarly journals ANALYSIS OF CRYOСONSERVED SPERM OF SIRES OF THE LEBEDINIAN BREED AND ORIGINAL BROWN CATTLE OF GERMANY

2019 ◽  
Vol 58 ◽  
pp. 95-101
Author(s):  
V. I. Ladyka ◽  
Y. I. Sklyarenko ◽  
Y. M. Pavlenko ◽  
O. V. Sherbak
Keyword(s):  
Sperm Motility ◽  
Sperm Production ◽  
Long Term Storage ◽  
Brown Cattle ◽  
Cryopreserved Sperm ◽  
Analysis System ◽  
Quantitative Indicators ◽  
Term Storage

In recent years, in Ukraine, as well as around the world, some local breeds have been on the verge of extinction, resulting in irretrievable loss of valuable genes, reduction of genetic diversity. In order to avoid these problems, it is necessary to work on the conservation of animal genetic resources. The creation of banks for long-term storage of biological material is one of the methods of preserving the gene pool of local, small and indigenous breeds of animals. Sperm quality is one of the main factors that determines the success of insemination of heifers and cows. Comprehensive analysis of sperm includes a large number of qualitative and quantitative indicators, the accuracy of which is influenced by a number of objective and subjective factors. Currently, microscopic analysis of sperm motility and survival is used in production conditions. Such approaches are easy to implement, but their results can be subjective. Research using a computer-based sperm fertility analysis system is becoming a priority today. With the help of CASA-Sperm Vision it is possible to carry out a morphological analysis already during the study of sperm motility. The purpose of our research was to analyze the quality of deep-frozen sperm of sires of Lebedinian breed and original brown cattle of Germany in order to attract such genetic material in the conservation of local Lebedinian breed cattle. Materials and methods. In the study used cryopreserved sperm doses of five sires of Lebedinian breed and three sires of the original brown cattle of Germany. Researches of qualitative, quantitative and dynamic characteristics of sperm of bulls were carried out at the laboratory of biotechnology of reproduction of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS and production laboratory "Ukrainian genetic company" using computer analysis of sperm motility Sperm Analysis System Version 12 IVOS. It was determined that the average dose of cryopreserved sperm of bulls of the Lebedinian breed was 0.25 ml, and of the sires of the original brown cattle of Germany -0.21 ml. It was found that the sires of the original brown cattle of Germany had the best indicators of total sperm motility by 21.7% compared to the sires of the Lebedinian breed (55.7%). They also noted a greater number of sperm with straight-forward movement of 16.5%. (40.2% of the Lebedinian breed sires). The average concentration of sperm in one milliliter was 10.5 times higher in animals of the Lebedinian breed (compared to the sires of the original brown cattle of Germany (331 million/ml). Comparing the indicators of the average speed of the sperm on the average trajectory (VAP), it should be noted that the minimum value was 85.3 microns/sec, the maximum value for this indicator – 141.7 microns/sec. The results of the VSL study showed that the average value of this indicator of sperm in the studied sperm doses of sires of the Lebedinian breed was 102 microns/sec, of the original brown cattle of Germany – 75. Obtained opposite to the direction coefficients of the correlation depending on the origin, between total sperm motility and average sperm speed for the average trajectory, the average speed of the sperm on the real trajectory, the average deviation of the sperm, the degree of straightness of the directed movement of sperm. Summary. 1. The complex of researches provided an objective analysis of qualitative and quantitative indicators of cryopreserved sperm production of the bulls of original brown cattle of Germany and the Lebedinian breed. It was found that the quality of the studied sperm doses met the requirements of "Instructions for Artificial Insemination of Cows and Heifers" (Order of the Ministry of Agrarian Policy of Ukraine dated August 1, 2001 № 230). 2. The comparative analysis of sperm production of the original brown cattle of Germany and the Lebedinian breed for its long-term storage was carried out. It is established that the sperm of sires of the original brown cattle of Germany exhibit higher dynamic characteristics of movement, while the sperm of sires of the Lebedinian breed were inferior to them in these indicators. 3. The possibility of insemination of females with cryopreserved sperm of the studied bulls whose sperm production has been stored for more than 30 years has been proved.

scholarly journals PERANCANGAN APLIKASI MOBILE CATALOG MODEL RAMBUT DENGAN MENERAPKAN ALGORITMA CROCHEMORE PERRIN

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Sri Hardiyanti
Keyword(s):  
Positive Impact ◽  
Quality Of Services ◽  
Modern Times ◽  
Changing Trends ◽  
New Models ◽  
Age Appropriate ◽  
The Media ◽  
The Right ◽  
Made In

In modern times, barbershop aka men's shaving places is the main choice of modern men to style their hair. The results showed that the popularity of Barbershop itself is due to the trend of the haircut, the classic hairdo. Classic hairstyle trends are also supported by the media which has a positive impact on popularity, hair fashion as a priority, knowledge about fashion is getting better, pomade trends. In general, customers want to get the best quality of services provided.Changes in customer haircuts or Barbershop customers are usually caused by changing trends or because they want to find new models that are more age-appropriate. The haircut catalog is a book that contains information on various haircut models that are made in the form of pictures and names of haircuts, where the names of haircuts are generally made in English.The problem that can occur in cutting hair is the lack of desires of the customer to the results of the hair stylist cut, it happens because the customer does not understand or do not remember the name of the desired haircut model. Crochemore Perrin's algorithm factored the right pattern so that pattern = right pattern left pattern. The matching phase of this algorithm consists of two parts, first matching the right pattern character from left to right, then matching the left pattern from right to left.Keywords: Barbershop, Android, Catalog, Crochemore Perrin

Evaluation of milt quality of Clarias anguillaris (Linnaeus, 1758) Broodstock fed varying inclusion levels of wild hibiscus Azanza garckeana pulp meal

2020 ◽  
Vol 34 (1) ◽  
pp. 23-31
Author(s):  
I.J. Ochokwu ◽  
M.O. Nwabunike ◽  
G.N. Udeh
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Control Diet ◽  
Feeding Trial ◽  
Inclusion Level ◽  
Fish Tank

Feeding trial was carried out to evaluate sperm quality of Clarias anguillaris broodstock fed varying inclusion levels of goron tula, Azanza garckeana pulp meal. The fish (mean weight 500g) were randomly distributed in concrete tanks (2 x 2 x 1.2m) at nine fish/tank in triplicates. Five isonitrogenous diets (40% CP) were formulated with the pulp meal incorporated at the rate of 0, 5, 10, 15, and 20% of the diet. Fish were fed at 3% of body weight for 56days. At the end of the feeding trial, results showed that dietary A. garckeana significantly (p<0.05) improved the milt quality of C. anguillaris. The broodfish fed 20% A. garckeana inclusion level had the highest (p<0.05) milt volume, sperm motility duration, testes lobe lengths, and whole sperm count while the least values of these variables were observed in group fed with the control diet (0% inclusion level). The findings conclusively revealed that increase in the level of A. garckeana pulp meal in the diet resulted into increase in sperm quality of C. anguillaris. Thus, the pulp meal can be freely utilized in catfish production in terms of sperm quality for mass fingerling production. Keywords: A. garckeana, Clarias anguillaris, sperm count, motility duration, milt volume.

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