scholarly journals Study on the genetic diversity of wax trees (Rhus succedanea L.) in Northern mountainous region of Vietnam by SSR and SCoT markers

2021 ◽  
Vol 19 (3) ◽  
pp. 471-480
Author(s):  
Nguyen Hong Chien ◽  
Nguyen Thi Kim Linh ◽  
Trin Thi Kim My ◽  
Nguyen Xuan Truong ◽  
Nguyen Van Chung ◽  
...  
Keyword(s):  
Genetic Similarity ◽  
Geographical Origin ◽  
Molecular Level ◽  
Tree Breeding ◽  
Similarity Coefficient ◽  
Breeding Program ◽  
High Yield ◽  
High Similarity ◽  
Tree Breeding Program ◽  
High Level

Wax tree has been cultivated to get lacquer, a valuable source of material that is necessary for many industries and handicrafts. Evaluating not only phenotype but also genotype is essential in order to increase the efficiency of new breeding program. In this study, the genetic relationship at the molecular level of 90 wax trees collected in three regions, i.e., Tam Nong, Thanh Son (Phu Tho) and Chiem Hoa (Tuyen Quang), was analyzed by SSR and ScoT markers. The results revealed a significant diversity among the individuals, with similarity coefficient from 0.41 to 0.98. The sample group collected in Chiem Hoa was significantly different from that in Tam Nong. Most of samples which had a high level of genetic similarity, from 0.95 to 0.98, were pairs of samples at the same geographical origin. The high similarity degree of these samples is likely due to the practice of selecting seeds from high-yield wax tree to be kept for seeds. These results provide the necessary information for new wax tree breeding program.

scholarly journals EVALUATION OF GENETIC DIVERSITY IN CACAO COLLECTED FROM KOLAKA, SOUTHEAST SULAWESI, USING SSR MARKERS

2016 ◽  
Vol 16 (2) ◽  
pp. 71
Author(s):  
Rubiyo Rubiyo ◽  
Nur Kholilatul Izzah ◽  
Indah Sulistiyorini ◽  
Cici Tresniawati
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Similarity Coefficient ◽  
Breeding Program ◽  
New Variety ◽  
Average Value ◽  
The Third ◽  
Ctab Method

Kolaka, which is located in Southeast Sulawesi, has long been known as one of cacao production centers in Indonesia. Therefore, many different cacao germplasms can be found in this region. The study aimed to evaluate genetic diversity and relationships of 12 cacao genotypes collected from Kolaka. Genomic DNA was extracted by using a modified CTAB method. Meanwhile, genetic diversity was analyzed based on 16 SSR markers, which then separated by 6% non-denaturing polyacryl-amide gel electrophoresis. The result showed that all of those markers, 14 markers exhibited polymorphism and subsequently used for data analysis using NTSYS and PowerMarker program. About 70 different alleles were generated from 12 cacao genotypes analyzed with an average of 5 alleles per locus. Average value of polymorphism information content (PIC) resulted in this study was 0.59. The cluster analysis using UPGMA method based on the genetic similarity coefficient revealed that all cacao genotypes were separated into three major groups. The first group consisted of five cacao genotypes, the second one held four cacao genotypes, whereas the third group contained three genotypes. This result indicates that three genotypes that clustered separately from the others could be used as a good clonal candidate for cacao breeding program. The information resulted from this present study would be useful for future cacao breeding program, especially in efforts to release a new variety.

scholarly journals EVALUATION OF GENETIC DIVERSITY IN CACAO COLLECTED FROM KOLAKA, SOUTHEAST SULAWESI, USING SSR MARKERS

2016 ◽  
Vol 16 (2) ◽  
pp. 71
Author(s):  
Rubiyo Rubiyo ◽  
Nur Kholilatul Izzah ◽  
Indah Sulistiyorini ◽  
Cici Tresniawati
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Similarity Coefficient ◽  
Breeding Program ◽  
New Variety ◽  
Average Value ◽  
The Third ◽  
Ctab Method

Kolaka, which is located in Southeast Sulawesi, has long been known as one of cacao production centers in Indonesia. Therefore, many different cacao germplasms can be found in this region. The study aimed to evaluate genetic diversity and relationships of 12 cacao genotypes collected from Kolaka. Genomic DNA was extracted by using a modified CTAB method. Meanwhile, genetic diversity was analyzed based on 16 SSR markers, which then separated by 6% non-denaturing polyacryl-amide gel electrophoresis. The result showed that all of those markers, 14 markers exhibited polymorphism and subsequently used for data analysis using NTSYS and PowerMarker program. About 70 different alleles were generated from 12 cacao genotypes analyzed with an average of 5 alleles per locus. Average value of polymorphism information content (PIC) resulted in this study was 0.59. The cluster analysis using UPGMA method based on the genetic similarity coefficient revealed that all cacao genotypes were separated into three major groups. The first group consisted of five cacao genotypes, the second one held four cacao genotypes, whereas the third group contained three genotypes. This result indicates that three genotypes that clustered separately from the others could be used as a good clonal candidate for cacao breeding program. The information resulted from this present study would be useful for future cacao breeding program, especially in efforts to release a new variety.

scholarly journals A TREE-BREEDING PROGRAM FOR CANADA

1951 ◽  
Vol 27 (2) ◽  
pp. 164-170 ◽  
Author(s):  
I. C. M. Place
Keyword(s):  
Tree Breeding ◽  
Breeding Program ◽  
Tree Breeding Program

scholarly journals 333 Evaluation of New Elms from China for Stress Tolerance

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 500D-500
Author(s):  
A.M. Shirazi ◽  
G.H. Ware
Keyword(s):  
Stress Tolerance ◽  
Heat Tolerance ◽  
Cold Hardiness ◽  
Urban Landscape ◽  
Tree Breeding ◽  
Breeding Program ◽  
Dutch Elm Disease ◽  
Leaf Beetles ◽  
Tree Breeding Program ◽  
And Performance

The high resistance of Chinese elms to Dutch elm disease and elm leaf beetles makes them excellent trees for the urban landscape. There are many new Chinese elms being developed through the tree breeding program at The Morton Arboretum. Many new elms are already on the market or will be available soon from nurseries. There is little known about the stress tolerance, e.g., cold, heat, drought, etc., of new elms from China. The objectives of this study were 1) to determine the midwinter cold hardiness of new Chinese elms and 2) to determine the leaf heat tolerance and stem cold hardiness of new elms from the nursery. The stem cold hardiness of nine new elms from China was evaluated in Feb. 1998 and 1999 from the elm breeding program at The Morton Arboretum by using an artificial freezing test. The LT50 (the temperature at which 50% of the tissues were killed) of the most to least hardy genuses for Feb. 1998 were Ulmus macrocarpa (> -36 °C), U. wilsoniana # 673 (-34 °C), U. parvifolia R-89-120 (-34 °C), U. wilsoniana # 669 (-34 °C), U. wilsoniana # 997 (-33.8 °C), U. szechuanica (-30 °C), U. gaussenii R-94-85D (-30.7 °C), U. bergmanniana var. lasiophylla R-9422SD (-27.7 °C), and U. castaneifolia #R-9411-11-SD (-25.9 °C). Four new elms from Schmidt Nursery, Boring, Ore., were also evaluated for leaf heat tolerance in August and cold hardiness in Dec. 1998 and Feb. 1999. The LT50 of cold hardiness of stem tissues of cultivars in December were U. parvifolia `Emer 1' P.P. 7551, Athena® elm (-23.5 °C), U. parvifolia `Emer 1' P.P. 7552 Allee® (-26.5 °C), U. Accolade™ (-33.5 °C), and U. Danada Charm™ elm (-31 °C). The LT50 of the most to least heat-tolerant cultivars were U. parvifolia `Emer 1' P.P. 7551, Athena® elm (53.8 °C), U. parvifolia `Emer 1' P.P. 7552 Allee® (52.1 °C), U. Accolade™ (50.8 °C), and U. Danada Charm™ elm (50.6 °C). Growth, dormancy development, spring budbreak, and performance of these cultivars will be compared.

scholarly journals Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus

2012 ◽  
Vol 48 (Special Issue) ◽  
pp. S43-S48 ◽  
Author(s):  
J. Polák ◽  
J. Kumar ◽  
B. Krška ◽  
M. Ravelonandro
Keyword(s):  
Tree Breeding ◽  
Fruit Tree ◽  
Eating Quality ◽  
Plum Pox Virus ◽  
Infection Pressure ◽  
Public Sector Research ◽  
The Usa ◽  
Chlorotic Leaf ◽  
High Level ◽  
And Control

Commercialisation of Biotech/GM (Biotech) crops started in 1995. Not only field crops, but also horticultural transgenic crops are under development and are beginning to be commercialised. Genetic engineering has the potential to revolutionise fruit tree breeding. The development of transgenic fruit cultivars is in progress. Over the past 20 years an international public sector research team has collaborated in the development of HoneySweet plum which is highly resistant to Plum pox virus (PPV) the most devastating disease of plums and other stone fruits. HoneySweet was deregulated in the USA in 2010. HoneySweet (aka C5) has been evaluated for eleven years (2002–2012) in a regulated field trial in the CzechRepublic for the resistance to PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV), all of them being serious diseases of plum. Even under the high and permanent infection pressure produced through grafting, PPV has only been detected in HoneySweet trees in several leaves and fruits situated close to the point of inoculum grafting. The lack of infection spread in HoneySweet demonstrates its high level of PPV resistance. Co-infections of PPV with PDV and/or ACLSV had practically no influence on the quantity and quality of HoneySweet fruit which are large, sweet, and of a high eating quality. In many respects, they are superior to the fruits of the well-known cultivar Stanley. Many fruit growers and fruit tree nurseries in the CzechRepublic are supportive of the deregulation of HoneySweet plum to help improve the plum production and control the spread of PPV.

scholarly journals Multi-curie production of gallium-68 on a biomedical cyclotron and automated radiolabelling of PSMA-11 and DOTATATE

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Helge Thisgaard ◽  
Joel Kumlin ◽  
Niels Langkjær ◽  
Jansen Chua ◽  
Brian Hook ◽  
...  
Keyword(s):  
Radiochemical Purity ◽  
High Yield ◽  
Clinical Use ◽  
Automated Production ◽  
Automated Method ◽  
Gallium 68 ◽  
High Yields ◽  
High Level ◽  
Mev Protons ◽  
Very High

Abstract Background With increasing clinical demand for gallium-68, commercial germanium-68/gallium-68 ([68Ge]Ge/[68Ga]Ga) generators are incapable of supplying sufficient amounts of the short-lived daughter isotope. In this study, we demonstrate a high-yield, automated method for producing multi-Curie levels of [68Ga]GaCl3 from solid zinc-68 targets and subsequent labelling to produce clinical-grade [68Ga]Ga-PSMA-11 and [68Ga]Ga-DOTATATE. Results Enriched zinc-68 targets were irradiated at up to 80 µA with 13 MeV protons for 120 min; repeatedly producing up to 194 GBq (5.24 Ci) of purified gallium-68 in the form of [68Ga]GaCl3 at the end of purification (EOP) from an expected > 370 GBq (> 10 Ci) at end of bombardment. A fully automated dissolution/separation process was completed in 35 min. Isolated product was analysed according to the Ph. Eur. monograph for accelerator produced [68Ga]GaCl3 and found to comply with all specifications. In every instance, the radiochemical purity exceeded 99.9% and importantly, the radionuclidic purity was sufficient to allow for a shelf-life of up to 7 h based on this metric alone. Fully automated production of up to 72.2 GBq [68Ga]Ga-PSMA-11 was performed, providing a product with high radiochemical purity (> 98.2%) and very high apparent molar activities of up to 722 MBq/nmol. Further, manual radiolabelling of up to 3.2 GBq DOTATATE was performed in high yields (> 95%) and with apparent molar activities (9–25 MBq/nmol) sufficient for clinical use. Conclusions We have developed a high-yielding, automated method for the production of very high amounts of [68Ga]GaCl3, sufficient to supply proximal radiopharmacies. The reported method led to record-high purified gallium-68 activities (194 GBq at end of purification) and subsequent labelling of PSMA-11 and DOTATATE. The process was highly automated from irradiation through to formulation of the product, and as such comprised a high level of radiation protection. The quality control results obtained for both [68Ga]GaCl3 for radiolabelling and [68Ga]Ga-PSMA-11 are promising for clinical use.

708 A NEW DIAGNOSTIC PARADIGM FOR LARYNGOPHARYNGEAL REFLUX DISEASE: CORRELATION OF IMPEDANCE-PH MONITORING AND DIGITAL REFLUX SCINTIGRAPHY RESULTS

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Jin-soo Park ◽  
Oleksandr Khoma ◽  
Hans Van Der Wall ◽  
Gregory Falk
Keyword(s):  
Laryngopharyngeal Reflux ◽  
Ph Monitoring ◽  
Acid Reflux ◽  
High Rate ◽  
High Yield ◽  
Time Activity ◽  
Proximal Esophagus ◽  
Laryngopharyngeal Reflux Disease ◽  
High Level ◽  
No Gold Standard

Abstract   No gold-standard investigation exists for laryngopharyngeal reflux (LPR). Multichannel intraluminal impedance (MII)-pH testing has uncertain utility in LPR. Meanwhile, reflux scintigraphy allows immediate and delayed visualisation of tracer reflux in the esophagus, pharynx, and lungs. The present study aimed to correlate MII-pH and scintigraphic reflux results in patients with primary LPR. Methods Consecutive patients with LPR underwent MII-pH and scintigraphic reflux studies. Abnormal values for MII-pH results were defined from existing literature. MII-pH and scintigraphic data were correlated. Results 105 patients with LPR (31 males (29.5%), median age 60 years (range: 20–87)) were studied. Scintigraphic reflux was seen in the pharynx in 94 (90.4%), and in the proximal esophagus in 94 (90.4%). Delayed scintigraphic contamination of the pharynx was seen in 101 patients (96.2%) and in the lungs of 56 patients (53.3%). Abnormal reflux was seen in the distal esophagus in 12.4%, proximal esophagus in 25.7%, and in the pharynx in 82.9%. Patients with poor scintigraphic clearance had higher Demeester scores (p = 0.043), more proximal reflux episodes (p = 0.046), more distal acid reflux episodes (p = 0.023), and longer bolus clearance times (p = 0.002). Conclusion Reflux scintigraphy has a high yield in LPR patients. Scintigraphic time-activity curves correlated with validated MII-pH results. A high rate of pulmonary microaspiration was found in LPR patients. This study demonstrated a high level of pharyngeal contamination by scintigraphy and MII-pH, which supports the use of digital reflux scintigraphy in diagnosing LPR.

scholarly journals Morphological and Molecular Characterization of Major Quince Cultivars from Turkey

2016 ◽  
Vol 44 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Hasan PINAR ◽  
Suat KAYMAK ◽  
Serif OZONGUN ◽  
Aydın UZUN ◽  
Mustafa UNLU ◽  
...  
Keyword(s):  
Genetic Similarity ◽  
Fruit Weight ◽  
Asia Minor ◽  
Diversity Analysis ◽  
Cydonia Oblonga ◽  
Fruit Characteristics ◽  
Rosaceae Family ◽  
High Level ◽  
Morphological And Molecular Characterization

Quince (Cydonia oblonga Mill.) belongs to the Rosaceae family is native to south-eastern Europa and Asia Minor. It is generally used for table consumption and processed into jam, jelly and marmalade. It is also used as dwarfing rootstock for pear cultivars. In the present study, fruit characteristics and genetic diversity of 17 quince cultivars from Turkey were investigated. For fruit characteristics, 'Bardacik' had the highest fruit weight. The highest SSC/Acidity ratio were obtained from 'Osmancik'. There was high level of variation in fruit characteristics among cultivars. In molecular analysis, totally 133 bands were obtained from 23 sequence-related amplified polymorphism (SRAP) primer combinations and 67 of them were polymorphic. Genetic similarity of 17 cultivars was between 0.78 and 1.00. Two cultivars ('Sekergevrek' and '32S04') were found to be more distinct from the other cultivars genetically. This study showed that there was low level of genetic variation most of quince cultivars grown in Turkey. SRAP markers firstly used in quince with this study indicating that it can be used for characterization and diversity analysis of quince.

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