scholarly journals Peculiarities of Changing Bioregulators and Organic Acids in the Serum of Blood and Amniothic Fluid of Women with Spontaneous Preterm Labor

2018 ◽  
Vol 73 (6) ◽  
pp. 361-367 ◽  
Author(s):  
Irina I. Krukier ◽  
Valeriya V. Avrutskaya ◽  
Marina A. Levkovich ◽  
Elena V. Narezhnaya ◽  
Georgiy V. Smolyaninov ◽  
...  
Keyword(s):  
Organic Acids ◽  
Amniotic Fluid ◽  
Blood Serum ◽  
Premature Birth ◽  
Preterm Labor ◽  
Solid Phase ◽  
Biological Fluids ◽  
Prognostic Indicators ◽  
Control Group

Background: Premature birth is currently a serious problem for obstetrician-gynecologists. Most researcher consider this problem from the standpoint of metabolome disturbances.Aims: determination of the level of bioactive regulators and organic acids with physiological and complicated pregnancy, ending in timely and spontaneous preterm labor, in blood serum and amniotic fluid and revealing the presence of their relationship for the development of premature birth and the condition of newborns.Materials and methods: 72 women were examined, 40 of them with a physiological pregnancy (control group) and 32 with premature labor (the main group). All studies were carried out in the blood serum and amniotic fluid of women of these groups. The content of TGFβ was determined by the method of solid-phase enzyme immunoassay. Identification and quantitative determination of citric and succinic acids were carried out by the method of capillary electrophoresis.Results: TNFα production in the blood serum of women with preterm labor, decreased by 38%, and the level of citric and succinic acids by 33 and 30%, respectively, compared with the control group. In the amniotic fluid the level of TNFα was increased 2.5 times and TGFβ ― 2.2 times, citric acid ― 79%.Conclusions: The revealed imbalance in the level of cytokines and organic acids studied in that biological fluids can evidently affect the fetal condition, which can be considered as possible markers of perinatal injuries and prognostic indicators of the state of the newborn.

scholarly journals Endogenous Lactoferrin in the Assessment of Ferrokinetics in Children with Iron Deficiency States

2019 ◽  
pp. 44-50
Author(s):  
N. N. Klimkovich ◽  
G. P. Zubritskaya ◽  
E. I. Venskaya ◽  
A. G. Kutko ◽  
A. S. Skorobogatova ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Serum ◽  
Biological Fluids ◽  
Laboratory Data ◽  
Deficiency Anemia ◽  
Control Group ◽  
Healthy Children ◽  
Physiological Mechanisms ◽  
Latent Iron Deficiency

Objective: to study the concentration of endogenous lactoferrin in children with iron deficiency states before and during the treatment. Material and methods . The clinical and laboratory data of 31 patients with iron deficiency states (24 patients with iron deficiency anemia and 7 with latent iron deficiency) aged 0-17 (the median age was 9.7) and 7 healthy children (the median age was 12.4) who made up the control group have been analyzed. Hemogram and biochemical blood test were done according to the standard method. The concentration of endogenous LF in the blood serum was determined by the method of enzyme immunoassay using commercial test systems for the quantitative determination of human LF in the biological fluids (Elabscience) on the universal VICTOR2TM analyzer (Perkin Elmer, USA). Results . The study of ferrokinetics and LF concentration in the blood serum of the children with iron deficiency states has been performed. It has been shown that the children with iron deficiency reveal an increased LF level in the blood serum, which has variable values depending on age. The highest levels of the serum LF content are characteristic of patients under the age of three months, which is associated with the physiological mechanisms of the adaptation process of the hematopoietic system in newborns and infants. The LF concentration in the blood serum associated with ferrotherapy is increased compared to the corresponding indices before the treatment. Conclusion . From the results of the study its follows that the content of LF in the blood serum increases in iron deficiency and is associated with ferrotherapy. The obtained results will be used in further research aimed at the diagnosis of iron deficiency and prediction of the response to iron therapy.

The role of cytokines and organic acids production in the blood serum and amniotic fluid in spontaneous preterm labor

2021 ◽  
Vol 9_2021 ◽  
pp. 60-65
Author(s):  
Krukier I.I. Krukier ◽  
Levkovich M.A. Levkovich ◽  
Avrutskaya V.V Avrutskaya ◽  
Churyukina E.V. Churyukina ◽  
Nikashina A.A. Nikashina ◽  
...  
Keyword(s):  
Organic Acids ◽  
Amniotic Fluid ◽  
Blood Serum ◽  
Preterm Labor

scholarly journals The health of children born to mothers who have had a threat of abortion

2019 ◽  
Vol 64 (5) ◽  
pp. 27-32
Author(s):  
A. V. Smirnova ◽  
N. Yu. Borzova ◽  
N. Yu. Sotnikova ◽  
A. I. Malyshkina ◽  
N. V. Kharlamova
Keyword(s):  
At Risk ◽  
Blood Serum ◽  
Premature Birth ◽  
Preterm Labor ◽  
Ureaplasma Urealyticum ◽  
Venous Blood ◽  
Intrauterine Infection ◽  
Mycoplasma Hominis ◽  
Control Group ◽  
Premature Labor

The risk of preterm labor is one of the leading complications of gestation which determines the high level of perinatal morbidity and mortality. Receptor for advanced glycation end product RAGE is considered to be an important participant of congenital and adaptive immunity and it can regulate the production of pro-inflammatory cytokines and chemokines. TNFa is a leading pro-inflammatory cytokine, it is produced in response to endotoxin. TGFß1 is the main mediator providing anti-inflammatory effect.Purpose. To identify the features of the health status of newborns after the threat of termination of pregnancy in 22–27 weeks, the content of sRAGE and cytokines in women at risk of preterm labor depending on perinatal pathology in children.Materials and methods. We examined 120 pregnant women at 22–27 weeks of gestation, they were divided into 2 groups: the main group (80 women at risk of preterm labor) and the control group (40 women with uncomplicated pregnancy). We determined the content of sRAGE, TGFβ1 и TNFα cytokines, and antibodies to Chlamydia trachomatis, Mycoplasma hominis и Ureaplasma urealyticum, simple herpes virus, cytomegalovirus, the virus of Epstein–Barr in the venous blood serum by ELISA.Results. The threat of preterm labor at 22–27 weeks increases the risk of perinatal hypoxic lesions of the CNS in newborn 1,5 times (relative risk (RR) 1.43; 95% confidence interval – CI 1.14–1.78). A premature birth, after the threat of preterm labor at 22–27 weeks, increases the risk of perinatal damage to the CNS more than five times (RR 5.13; 95% CI 2.47–10,69), and the risk of intrauterine infection is increased four times (RR 4.08; 95% CI 2.54–6.54). Low content of sRAGE in patients with threat of premature labor at 22–27 weeks is a predictor of development of perinatal hypoxic lesions of the CNS.Conclusion. Low content of sRAGE in the blood serum of patients at risk of premature labor at 22–27 weeks is a predictor of development of perinatal hypoxic lesions of the CNS. A decreased concentration of TGFβ1 cytokine in the blood of women at risk of premature birth is associated with intrauterine infection in newborns.

scholarly journals Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2194
Author(s):  
Kamil Łuczykowski ◽  
Natalia Warmuzińska ◽  
Sylwia Operacz ◽  
Iga Stryjak ◽  
Joanna Bogusiewicz ◽  
...  
Keyword(s):  
Thin Film ◽  
Bladder Cancer ◽  
High Performance ◽  
Biomarker Discovery ◽  
Solid Phase ◽  
Biological Fluids ◽  
Reversed Phase ◽  
Control Group ◽  
Metabolic Evaluation ◽  
Metabolomic Profiling

Bladder cancer (BC) is a common malignancy of the urinary system and a leading cause of death worldwide. In this work, untargeted metabolomic profiling of biological fluids is presented as a non-invasive tool for bladder cancer biomarker discovery as a first step towards developing superior methods for detection, treatment, and prevention well as to further our current understanding of this disease. In this study, urine samples from 24 healthy volunteers and 24 BC patients were subjected to metabolomic profiling using high throughput solid-phase microextraction (SPME) in thin-film format and reversed-phase high-performance liquid chromatography coupled with a Q Exactive Focus Orbitrap mass spectrometer. The chemometric analysis enabled the selection of metabolites contributing to the observed separation of BC patients from the control group. Relevant differences were demonstrated for phenylalanine metabolism compounds, i.e., benzoic acid, hippuric acid, and 4-hydroxycinnamic acid. Furthermore, compounds involved in the metabolism of histidine, beta-alanine, and glycerophospholipids were also identified. Thin-film SPME can be efficiently used as an alternative approach to other traditional urine sample preparation methods, demonstrating the SPME technique as a simple and efficient tool for urinary metabolomics research. Moreover, this study’s results may support a better understanding of bladder cancer development and progression mechanisms.

MONITORING OF MALIGNANT GROWTH DYNAMICS BY STRUCTURAL ANALYSIS OF NON-CELLULAR TISSUE

2021 ◽  
pp. 89-96
Author(s):  
V.N. Shabalin ◽  
S.N. Shatokhina ◽  
M.G. Dedova
Keyword(s):  
Blood Serum ◽  
Laryngeal Cancer ◽  
Solid Phase ◽  
Biological Fluids ◽  
Treatment Options ◽  
Developmental Phase ◽  
Malignant Growth ◽  
Active Growth ◽  
Main Research ◽  
Degree Of Anisotropy

The authors examined the composition of biocrystalline structures (anisomorphones) of blood serum in patients with laryngeal cancer. Such structures are formed when blood serum becomes solid, i.e. during its marginal dehydration. The revealed anisomorphones represent three types of marker structures: a marker of a malignant tumor active growth (the aggregation of macroferolite and granular microspherolite with the same degree of anisotropy); a marker of a degenerative-dystrophic process (the aggregation of a macrospherolite with a low degree of anisotropy and microspherolite with a high degree of anisotropy); a marker of a malignant growth progression (a wavy microspherolite without aggregation). The aim of the study is to identify diagnostic markers of the malignant process activity in the solid phase structures of the blood serum in patients with laryngeal cancer and to assess their importance for choosing an effective therapy. Materials and Methods. Marginal dehydration of blood serum was used as the main research method. It is a part of the "Litos-system" diagnostic technology (Marketing authorization FS No. 155, of 2009). Results. It has been shown that the developmental phase of laryngeal cancer (active growth or degenerative-dystrophic process) is an important criterion for choosing treatment options. Surgical treatment is the most effective during the degenerative-dystrophic tumor process, while radiation therapy is preferable during the active phase of malignant growth. Key words: laryngeal cancer, blood serum, marginal dehydration of biological fluids, markers of tumor growth activity. Исследован состав биокристаллических структур (анизоморфонов) сыворотки крови больных раком гортани, которые формируются при переходе сыворотки крови в твердую фазу в процессе ее краевой дегидратации. Выявленные анизоморфоны представляют собой три вида маркерных структур: маркер активного роста злокачественной опухоли – агрегация макросферолита и зернистого микросферолита с одинаковой степенью анизотропии; маркер дегенеративно-дистрофического процесса – агрегация макросферолита с низкой степенью анизотропии и микросферолита с высокой степенью анизотропии; маркер прогрессии злокачественного роста – волнистый микросферолит вне агрегации. Цель – выявить диагностические маркеры активности злокачественного процесса в структурах твердой фазы сыворотки крови больных раком гортани и оценить их значение для выбора эффективного вида лечения. Материалы и методы. В качестве основного метода исследования использован метод краевой дегидратации сыворотки крови, являющийся разделом диагностической технологии «Литос-система» (Разрешение ФС № 155 от 2009 г. на применение в клинической практике). Результаты. Показано, что фаза развития рака гортани (активный рост или дегенеративно-дистрофический процесс) служит важным критерием выбора вида лечения: в фазу дегенеративно-дистрофического процесса опухоли наиболее благоприятный эффект дает хирургическое лечение, а в период активной фазы злокачественного роста – лучевая терапия. Ключевые слова: рак гортани, сыворотка крови, краевая дегидратация биологических жидкостей, маркеры фазы активности опухолевого роста.

Solid-phase radioimmunoassay for morphine, with use of an affinity-purified morphine antibody.

1978 ◽  
Vol 24 (2) ◽  
pp. 339-342 ◽  
Author(s):  
M Steiner ◽  
J L Spratt
Keyword(s):  
Affinity Chromatography ◽  
Correlation Coefficient ◽  
Solid Phase ◽  
Rapid Determination ◽  
Biological Fluids ◽  
Inhibitory Effect ◽  
Major Metabolite ◽  
Solid Phase Radioimmunoassay ◽  
Related Compounds

Abstract Morphine antibody purified by affinity chromatography was used to develop a solid-phase radioimmunoassay for morphine in polystyrene tubes. The tubes are coated with an appropriate concentration of the purified antibody, rinsed three times with buffered saline, and stored at -15 degrees C. Using tritiated dihydromorphine, we determined competitive morphine binding by difference when the radioactivity in the assay supernates was measured after incubation (1 h, 37 degrees C). Five standard curves, with use of serum equivalents of morphine ranging from 0 to 6 mug/liter, were linear and had a mean correlation coefficient of 0.98. Uncer conditions of the assay, levorphanol was comparable to morphine in its inhibitory effect on binding of labeled dihydromorphine, whereas dextrorphan was essentially inactive. Morphine-3-glucuronide, a major metabolite, is 55-fold less inhibitory in terms of its capacity to displace the radiolabel. We believe that the sensitivity of the technique, coupled with the simplicity of nonseparatory sampling, renders the system suitable for rapid determination of morphine and related compounds in biological fluids.

Simultaneous magnetic dispersive micro solid phase extraction of valsartan and atorvastatin using a CMC-coated Fe3O4 nanocomposite prior to HPLC-UV detection: multivariate optimization

2019 ◽  
Vol 43 (43) ◽  
pp. 16950-16959 ◽  
Author(s):  
Ali Azadi ◽  
Shahin Ahmadi
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Biological Fluids ◽  
Uv Detection ◽  
Phase Extraction ◽  
Multivariate Optimization ◽  
Uv Detector ◽  
Practical Procedure

In this study, a sensitive, rapid, accurate and practical procedure is established for determination of atorvastatin and valsartan from human biological fluids by dispersive micro solid phase extraction (D-μ-SPE) combined with HPLC-UV detector.

scholarly journals Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction

DYNA ◽  
2020 ◽  
Vol 87 (212) ◽  
pp. 26-30
Author(s):  
Laura Marcela Trujillo Vargas ◽  
Yesid Hernan Vélez Salazar ◽  
Margarita Enid Ramírez Carmona
Keyword(s):  
Oxalic Acid ◽  
Organic Acids ◽  
Solid Phase ◽  
Fermentation Broth ◽  
Preparation Technique ◽  
Liquid Chromatograph ◽  
Ion Exclusion ◽  
Validation Parameters ◽  
Fermentation Broths

This work describes a method for the simultaneous determination of organic acids in fungi fermentation broths. The sample preparation technique and the chromatographic conditions were optimized to enable the monitoring of the fermentation process. The determination of the citric and oxalic acid was done with a high resolution liquid chromatograph adapted to a detector with diode arrangement, an IC-Pak Ion-Exclusion 7μm (7.8 x 300 mm) column and a mobile phase of 0.001N H2SO4 with isocratic elusion. The validation parameters show efficiency, adequate linearity, and standard deviation values (%RSD) from 0.018% and 4.650%. the quantification limits (LDC) were 50.76 mg/L for citric acid and 20.18 mg/L for oxalic acid and the detection limits (LDD) were 0.6 mg/L for both acids. The method was applied in the analysis of organic acids in fermentation broths of Aspergillus niger.

Emission Spectrometric Determination of Trace Elements in Biological Fluids

1971 ◽  
Vol 25 (1) ◽  
pp. 53-56 ◽  
Author(s):  
William Niedermeier ◽  
James H. Griggs ◽  
Richard S. Johnson
Keyword(s):  
Experimental Data ◽  
Trace Elements ◽  
Sample Preparation ◽  
Blood Serum ◽  
Biological Fluids ◽  
Spectrometric Method ◽  
Direct Reading ◽  
Iron Aluminum ◽  
Dc Arc

An emission spectrometric method of analysis is described, in which trace quantities of copper, iron, aluminum, barium, manganese, nickel, cesium, tin, strontium, chromium, zinc, lead, molybdenum, and cadmium were determined in blood serum. The sample preparation, starting with 2.0 ml of blood serum, is discussed in detail. The source of excitation was a 10 A dc arc. Quantitation was achieved with a direct reading emission spectrometer. The metal concentration, in micrograms per 100 ml of blood serum, was calculated from the experimental data by means of a computer.

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