scholarly journals Effect of pollen viability and method of storage on seed set, seed yield and seed quality under in-vivo condition of sunflower

2016 ◽  
Vol 11 (2) ◽  
pp. 318-321
Author(s):  
V. K. DESHPANDE ◽  
PRIYA KIVADASANNAVAR
Keyword(s):  
Seed Yield ◽  
Seed Quality ◽  
Seed Set ◽  
Pollen Viability

Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

2021 ◽  
pp. 1-10
Author(s):  
Sourbh Kumar ◽  
Uttam Chandel ◽  
Satish Kumar Guleria
Keyword(s):  
Doubled Haploid ◽  
Seed Set ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Haploid Inducer ◽  
Haploid Production ◽  
Maize Genotypes ◽  
Different Doses

Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

scholarly journals Effect of Pollen Genotype, Temperature and Period of Storage on In Vitro Germinability and In Vivo Seed Set in Chrysanthemum—Preliminary Study

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2395
Author(s):  
Natalia Miler ◽  
Anita Wozny
Keyword(s):  
Seed Set ◽  
Pollen Viability ◽  
Chrysanthemum Morifolium ◽  
Pollen Storage ◽  
Cross Breeding ◽  
Pollen Donor ◽  
Pollen Germinability ◽  
Fresh Pollen

Among many challenges in chrysanthemum cross-breeding, the access to viable pollen for hybridization of cultivars distant in location and different in flowering time is required. Low pollen viability along with incompatibility are mainly responsible for low seed set in modern chrysanthemum cultivars. The aim of the study was to test various temperatures and periods of pollen storage of Chrysanthemum × morifolium in order to elaborate the method of chrysanthemum pollen preservation for cross-breeding purposes. In the first experiment, in vitro pollen germination of four cultivars was investigated following storage at 20 °C, 4 °C, −20 °C, and −80 °C, for one, four, and eight weeks. The second experiment focused on in vivo seed set after one week pollen treatment with 20 °C, 4 °C, −20 °C, and −80 °C (three pollen donor cultivars tested). Pollen in vitro germinability, as well as seed set efficiency, was generally low and cultivar dependent. Independent of the period of storage, stored pollen germinability was lower (5.30–6.63%) than fresh pollen (8.15%). Incubation of pollen in −80 °C significantly increased pollen germinability (9.80%), as well as seed set efficiency in comparison to control (19.28% and 10.21%, respectively) provided the cultivars are compatible. Among cultivars, the highest germinability of pollen was found in ‘Brda’ and ‘Donna’ (8.2% and 8.23%, respectively), while ‘Bydgoszczanka’ showed the lowest germinability (2.97%). There were also pollen genotype dependent effects in in vivo seed set efficiency, which was highest in ’Brda’ (17.57%) and much lower in ‘Jutrzenka’ and ‘Polka’ (1.34% and 0.39%, respectively), which contributed to the incompatibility of crossed cultivars rather than pollen viability.

scholarly journals 090 Pollen Viability in Inland Saltgrass

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 457A-457
Author(s):  
Scott Reid ◽  
Judy Harrington ◽  
Harrison Hughes
Keyword(s):  
Seed Set ◽  
Pollen Viability ◽  
Pollen Tubes ◽  
Aniline Blue ◽  
Golf Courses ◽  
Mine Spoils ◽  
Hand Pollination ◽  
Poor Seed

Distichlis spicata var. stricta (Torrey) Beetle is a native grass that tolerates salt, high pH, and some heavy metals. It has been proposed for use in several challenging environments, including mine spoils and salt-impacted areas of golf courses. But, its widespread use has been hindered by several factors, one of which is poor seed set. Because chromosome numbers are variable and some genotypes are aneuploids, there was concern that pollen viability in some genotypes was low. Pollen from several genotypes failed to germinate in vitro on four artificial media prepared with various levels of osmoticum. However, hand pollination in vivo resulted in profuse pollen germination for all genotypes tested. Germination on pollinated stigmas was observed at intervals beginning 2 h after pollination with a fluorescence microscope using aniline blue and acridine orange stains and in bright field using toluidine-O stain. Very young stigmas seemed unreceptive and, while pollen would germinate, the pollen tubes would not grow down through the style. On receptive stigmas, many pollen tubes grew down toward the egg and some reached it within 24 h. There was no evidence of impaired fertility. Aniline blue was the best method for observing pollen tube growth through the style, although toluidine-O was adequate for observing germination on the stigmatic surface.

Breeding for Seed Yield and Seed Quality in Smooth Bromegrass, Bromus inermis Leyss . 1

Crop Science ◽  
1970 ◽  
Vol 10 (5) ◽  
pp. 539-542 ◽  
Author(s):  
R. P. Knowles ◽  
D. A. Cooke ◽  
E. Buglass
Keyword(s):  
Seed Yield ◽  
Seed Quality ◽  
Bromus Inermis ◽  
Smooth Bromegrass

Spikelet fertility and heat shock transcription factor (Hsf) gene responses to heat stress in tolerant and susceptible rice (Oryza sativa L.) genotypes

2019 ◽  
Vol 157 (04) ◽  
pp. 283-299 ◽  
Author(s):  
C. Malumpong ◽  
S. Cheabu ◽  
C. Mongkolsiriwatana ◽  
W. Detpittayanan ◽  
A. Vanavichit
Keyword(s):  
Heat Stress ◽  
Pollen Germination ◽  
Seed Set ◽  
Oryza Sativa L ◽  
Flag Leaf ◽  
Pollen Viability ◽  
Spikelet Fertility ◽  
Anther Dehiscence ◽  
Spikelet Sterility ◽  
Plant Parts

AbstractThe reproductive stage of rice is the most sensitive to heat stress, which can lead to spikelet sterility. Thus, heat-tolerant and heat-susceptible genotypes were used to investigate their differences in terms of phenotypic responses and expression changes of Hsf genes at the pre-flowering stage under heat stress. Results clearly showed that panicles had the highest temperature compared with other plant parts under both natural and heated conditions. However, the temperatures of tolerant and susceptible genotypes were not significantly different. In terms of spikelet fertility, the tolerant lines M9962 and M7988 had high seed set because their anther dehiscence, pollen viability and pollen germination were only slightly affected. In contrast, the susceptible line Sinlek showed severe effects at all steps of fertilization, and the pollen viability of M7766 was slightly affected under heat stress but was more affected in terms of anther dehiscence and pollen germination. Both susceptible lines showed dramatically decreased seed set. In addition, the expression of six HsfA genes in the flag leaves and spikelets at the R2 stage of plants under heat stress showed different responses. Notably, expression of the HsfA2a gene was predominantly upregulated in the flag leaf and spikelets under heat stress in M9962. Therefore, it can be concluded that heat stress has severe effects on the stamen, and that different genotypes have different susceptibilities to heat stress.

scholarly journals Evaluation of Genotype, Environment, and Management Interactions on Fava Beans under Mediterranean Field Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1088
Author(s):  
Mohamed Houssemeddine Sellami ◽  
Antonella Lavini ◽  
Davide Calandrelli ◽  
Giuseppe De Mastro ◽  
Cataldo Pulvento
Keyword(s):  
Vicia Faba ◽  
Seed Yield ◽  
Yield Components ◽  
Seed Quality ◽  
Sandy Loam ◽  
Sowing Date ◽  
South Italy ◽  
Vicia Faba L ◽  
Fava Beans ◽  
Total Seed

Faba beans (Vicia faba L.), also known as fava beans, like other crops, are influenced by several factors: their genotype, environment, and management, as well as the interaction between these, have an important impact on seed yielding and seed quality traits. This study was conducted at three locations in South Italy between 2017 and 2019 to evaluate the sowing date effect on yield and yield components of three Vicia faba L., originating from cool climates. The results showed that seed yield (SY) and yield components declined with sowing delay. The crop’s environment (year × site) and management (sowing date) were found to explain 34.01% and 42.95% of the total seed yield variation, respectively. The data showed that the tested genotypes were positively influenced by the environment with sandy loam soil and early winter sowing date, resulting in either a greater number of SY and THS than in the other environment. The three faba bean genotypes showed tolerance to winter frost conditions in the two growing seasons.

Effect of varying seeding rates on hybrid and conventional summer rape performance in Manitoba

1992 ◽  
Vol 72 (3) ◽  
pp. 635-641 ◽  
Author(s):  
A. E. Van Deynze ◽  
P. B. E. McVetty ◽  
R. Scarth ◽  
S. R. Rimmer
Keyword(s):  
Protein Content ◽  
Chlorophyll Content ◽  
Seed Yield ◽  
Harvest Index ◽  
Dry Matter ◽  
Oil Content ◽  
Seed Quality ◽  
Seeding Rate ◽  
Seed Formation ◽  
Matter Production

To compare the effects of varying seeding rate on the agronomic performance, phenology and seed quality of hybrid and conventional summer rape cultivars, four hybrid and two conventional summer rape cultivars were seeded at 1.5, 3.0, 4.5, 6.0 and 9.0 kg ha−1 at two locations for 3 yr. The hybrid cultivars were, very importantly, 24% higher yielding and produced 50% more total dry matter than the conventional cultivars. The hybrid cultivars were, on average, 1.3% lower in seed oil content, 1.0% higher in seed protein content and equal in sum of oil and protein in the seed compared with the conventional cultivars. The hybrid cultivars were on average, 1.3 d later to 50% flowering and 1.1 d later to maturity than the average for the conventional cultivars, (i.e., equal or earlier to flowering and maturity than Regent). The hybrid cultivars were also 3.9% lower in harvest index and 1.3 ppm lower in chlorophyll content than the conventional cultivars. In spite of these differences, there were no significant cultivar-by-seeding-rate interactions, indicating that the hybrid and conventional cultivars responded similarly to varying seeding rate. Lodging, days to 50% flowering, days to maturity, harvest index, survival, oil content and protein content displayed significant linear responses to varying seeding rate. Stand at maturity, seed yield and total dry matter production displayed significant linear and quadratic responses to varying seeding rate. Varying seeding rate had no effect on seed formation period, the sum of oil and protein content, or chlorophyll content. A seeding rate of 6 kg ha−1 maximized seed yield for both hybrid and conventional summer rape cultivars.Key words: Brassica napus, canola, seed quality, agronomy, phenology

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