scholarly journals Phenotypic and Biochemical Characterization of a New Genotype of Satureja montana (L.)

Author(s):  
Costel VÎNĂTORU ◽  
Elena BARCANU ◽  
Ovidia Loredana DOBRE-AGAPIE ◽  
Ion GHERASE ◽  
Bianca TĂNASE ◽  
...  

Satureja Montana L. is an underrated, but very valuable species with multiple uses appreciated especially for its anti-inflammatory, antibacterial, antifungal and antioxidant properties. At VRDS Buzau was obtained a new genotype of winter savory with high content in volatile oils, minerals and with a yield potential of over 4.802 kg/plant after five years of cultivation. The content in volatile oils was determined using gas chromatography and the main component of the volatile oils were: thymol (83.04%), carvacrol (4.46%), o-cymene (4.21%) and other 13 components, in smaller percentages. The plant is perennial and can be grown for over 20 years.

2017 ◽  
Vol 5 (1) ◽  
pp. 77 ◽  
Author(s):  
Mongi Melki ◽  
Abir Gsouri ◽  
Mariem Bouhadida ◽  
Hnya Chograni ◽  
Mohsen Rezgui

Five Tunisian varieties of Kabuli chickpea were characterized based on agro morphological, molecular and biochemical parameters to investigate their genetic variability and yield potential. Randomized complete block design field trials were carried out in the upper semi-arid region of Kef in Tunisia during the 2013-2014 seasons. Data analysis showed significant differences between genotypes for several parameters. The results indicated that these genotypes could be set into two different groups. The first group composed of Bochra and Chetoui genotypes. Kasseb, Neyer and Beja1 were in the second group. Genotypes in each group were closely related to each other according to their common morphological characters such as pod number, one hundred seeds weight and yield.  Chetoui and Kasseb varieties are later in comparison to other varieties. Genetic diversity was studied using simple sequence repeat (SSR) markers. Four loci (TA64, TA71, TA96, TA194) were multiallelic. Whereas while two loci (TA72, GAA47) were monomorphic. Polymorphism analysis showed a phylogeny related to genotypes differentiation according to their relatives, origin and several morphological characters. Bochra variety had high amino acids content followed by Chetoui variety. All the varieties were deficient in sulfur amino acids. Chickpeas protein contents were variable and high ranging from 18% to 25%.


2022 ◽  
Vol 370 ◽  
pp. 131012
Author(s):  
Carolina Otero ◽  
Sebastián Miranda-Rojas ◽  
Felipe M. Llancalahuén ◽  
Juan A. Fuentes ◽  
Cristian Atala ◽  
...  

2009 ◽  
Vol 5 (2) ◽  
pp. 678-684 ◽  
Author(s):  
Mohammad Hadi Meshkatalsadat ◽  
Khadijeh Rabiei ◽  
Yaghub Shabaninejad

In this study, essential oils from different organs of Satureja bachtiarica Bunge were obtained by hydro-distillation. Quality and quantity of chemical composition of essential oils were determined by capillary gas chromatography and using gas-chromatography and mass spectrometric detection. The numbers of compounds were identified in the essential oils of leaves; stem and aerial parts were 45, 39 and 35, respectively. Also, carvacrol was the main component found in essential oils from leaves (39.3%), stem (39.4%) and aerial parts (67.88%) of S. bachtiarica in flowering stage. In addition, results showed that there were some different in compositions of the essential oils of different parts of S. bachtiarica and there are some minor components in each oil that are not present in the others parts. In addition chemical analysis of essential oils obtained from leaves and stem of S. bachtiarica were rich in oxygenated monoterepens (55.64% and 67.53%) while oxygenated monoterepen (77.22%) were the main class of compounds in the essential oils from aerial parts of S. bachtiarica. Results of this study showed that the essential oils from different organs of S. bachtiarica have a potential to be used as a new carvacrol source in drug and food industries.


2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Anna Végh ◽  
Tímea Bencsik ◽  
Péter Molnár ◽  
Andrea Böszörményi ◽  
Éva Lemberkovics ◽  
...  

The aims of our research include the phytochemical characterization of the essential oils extracted from different lavender species ( Lavandula vera L., L. intermedia L., L. pyrenaica DC., and L. stoechas subsp. stoechas) that are important from therapeutic and economic aspects, as well as the optimization of the tube dilution method for microbiological investigation of the effect of essential oils on the selected Pseudomonas strain. The chemical and percentage compositions of the essential oils were analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The main component of the essential oils in the majority of lavender species was linalool, while L-fencone was identified in the largest amount in L. stoechas subsp. stoechas. Tube dilution is one of the most frequently used methods for microbiological investigation. Its advantages are that it is easy to perform and provides information about minimum bactericidal concentration (MBC). Because of the lipophilic character of essential oils, the tube dilution method should be optimized for the detection of antibacterial activity of these plant extracts, and, therefore, emulsions containing 0.2% polysorbate 80 were prepared from the samples. In each of the eight investigated essential oils an inhibitory effect was detected, and MBC values were in the range 12.5-50 μL/mL in seven cases.


2021 ◽  
Author(s):  
Diana Loreta Paun ◽  
Alexandra Mirica

Pheochromocytomas and paragangliomas are rare neuroendocrine tumors, with genetic background in about 40% of cases, involving more than 30 susceptibility genes. The susceptibility genes can be divided into three main molecular clusters: pseudohypoxic, kinase signaling, and Wnt signaling. Biochemical characterization of these particular tumors should be integrated into the diagnostic algorithm because it can help apply personalized medicine principles and targeted therapy. These tumors can present with very different genotype-phenotype correlations, and their characterization can help the clinical practitioner make optimal clinical management decisions and prioritize genetic testing. This chapter summarizes the most important aspects of genetics and clinical characteristics, together with new genotype-phenotype correlation data.


2020 ◽  
Vol 2 (1) ◽  
pp. 35
Author(s):  
Carlo Camerlingo ◽  
Fabrizia D’Apuzzo ◽  
Marcella Cammarota ◽  
Sonia Errico ◽  
Marianna Portaccio ◽  
...  

Human periodontal ligament (PDL) is a membrane-like connective tissue interposed between the tooth root and the alveolar bone, the main component of which is represented by collagen fibers. During the early stage of application of orthodontic forces, different changes occur in PDL. For this reason, its characterization with conventional and non-conventional techniques can be extremely interesting. We investigated samples of PDL of orthodontic patients, aged between 13 and 21 years, using different experimental techniques. Morphological characterization of PDL samples was carried out by using a scanning electron microscope. Fourier-Transform Infrared (μ-FT-IR) and Raman (μ-RS) microspectroscopies were used for biochemical characterization of PDL samples. A biochemical characterization of PDL tissues with clear evidence of contributions from collagen, lipid and other protein was obtained. The analysis of Amide I and Amide III components was also performed, giving an indication of the protein secondary structure.


Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.


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