scholarly journals Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

2014 ◽  
Vol 42 (2) ◽  
pp. 431-439
Author(s):  
Erdogan E. HAKKI ◽  
Nurdan DOGRAR ◽  
Anamika PANDEY ◽  
Mohd. Kamran KHAN ◽  
Mehmet HAMURCU ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Durum Wheat ◽  
Molecular Data ◽  
Wheat Varieties ◽  
Elemental Analyses ◽  
Principle Coordinate Analysis ◽  
Elemental Characterization ◽  
Selection Of

Combination of elemental and protein studies along with molecular data using microsatellite markers may lead to the better and realistic determination of relatedness between the varieties and their populations. In this study, the extent of diversity among five Turkish durum wheat cultivars and their populations has been assessed using seven microsatellite markers and the elemental analysis together with the differences in their protein content.In molecular analysis, total 23 alleles have been obtained among all the genotypes with middling of 4.6 per primer. On employing UPGMA Dendrogram, Principle Coordinate Analysis (PCoA) and Winboot analyses, both inter and intra varietal polymorphic studies had shown similar clustering with minor differences. As a result of AMOVA performed, the extent of diversity was found to be higher among the genotypes (76%) in comparison to the variability within the genotypes (24%). In elemental analyses, ‘Selcuklu-97’ was found to be the most efficient variety with high content of several elements. Also, strong and positive correlation has been observed between magnesium- phosphorus, magnesium-sulphur and sulphur-sodium, while noteworthy negative correlation has been observed between sodium and zinc. The protein content of the genotypes was found in the range of 15.17-16.90%.The diversity revealed in durum genotypes can be employed in genetic expansion of the crop. These involved varieties may aid to avoid genetic attrition coming up from the landraces. The information provided can be utilized by breeders for appropriate selection of both, genetically and nutritionally efficient durum wheat varieties.

scholarly journals Genetic variability for coloured caryopses in common wheat varieties determined by microsatellite markers

2013 ◽  
Vol 49 (No. 3) ◽  
pp. 116-122 ◽  
Author(s):  
M. Musilová ◽  
V. Trojan ◽  
T. Vyhnánek ◽  
L. Havel
Keyword(s):  
Microsatellite Markers ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Breeding Programmes ◽  
New Gene ◽  
Yellow Coloration ◽  
Selection Of

Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. The caryopses of certain wheat genotypes contain antioxidants, i.e. anthocyanins or carotenoids, which cause purple, blue or yellow coloration. The first step before the introduction of these traits into individual wheat cultivars is the characterization of relationships and the possibility of new gene combinations. In this study, relationships among 24 genotypes with different types of caryopsis colour were investigated by means of microsatellite markers. Using 44 SSR (Simple Sequence Repeat) markers it was possible to detect a total of 184 alleles; on average, approximately 4 alleles were detected at a microsatellite locus. Using a set of 5 SSR markers (Xgwm636, Xbarc077, Xwmc262, Xgwm397 and Xwmc219) with PIC (polymorphic information content) values higher than 0.70, it was possible to differentiate among all the genotypes analysed. A dendrogram was created on the basis of all SSR markers, and showed that the genotypes were divided into two groups. Three, and one genotype with purple and blue caryopsis, respectively, belonged to one cluster, while the remaining twenty formed the second, greater cluster, which was subdivided into 2 sub-clusters: one of them involved genotypes with blue caryopses, and the other those with yellow and red caryopses. The genotype of tall wheatgrass (Thinopyrum ponticum), as a possible donor of genes responsible for blue caryopses, was also classified. These results can be used in wheat breeding programmes aimed at the selection of functional foodstuffs.

scholarly journals Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

2014 ◽  
Vol 42 (2) ◽  
Author(s):  
Erdogan E. HAKKI ◽  
Nurdan DOGRAR ◽  
Anamika PANDEY ◽  
Mohd. Kamran KHAN ◽  
Mehmet HAMURCU ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Wheat Varieties ◽  
Elemental Characterization

Selection of Dry Pea (Pisum Sativum) Varieties and their Performance by Comparison with Barley and Wheat in Mediterranean Environments

1994 ◽  
Vol 30 (4) ◽  
pp. 477-481
Author(s):  
A. Hadjicristodoulou
Keyword(s):  
Pisum Sativum ◽  
Durum Wheat ◽  
Protein Content ◽  
Arid Areas ◽  
Wheat Varieties ◽  
Mediterranean Countries ◽  
Supplementary Irrigation ◽  
Rainfed Conditions ◽  
Semi Arid ◽  
Selection Of

SUMMARYThe possibility of growing dry peas in the semi-arid areas of Mediterranean countries was assessed by comparing dry pea (Pisum sativum L.) varieties with the highest yielding barley and durum wheat varieties under rainfed conditions and with supplementary irrigation in Cyprus. The yield of barley cv. Kantara was greater than that of the best pea variety selected, PS 210713, especially under the driest conditions, but the differences between cereals and dry pea varieties were smaller when grown in areas where there was more rain or where supplementary irrigation was supplied. The protein content of the dry seeds and straw of the peas was higher than those of the cereals. It is concluded that selected varieties of dry peas could be grown in dry Mediterranean areas, particularly in rotation with barley or wheat.Pisum sativum L. para zonas mediterráneas

Molecular characterization of storage proteins for selected durum wheat varieties grown in different environments

2015 ◽  
Vol 61 ◽  
pp. 97-104 ◽  
Author(s):  
Olfa Daaloul Bouacha ◽  
Larbi Rhazi ◽  
Thierry Aussenac ◽  
Salah Rezgui ◽  
Sadok Nouaigui
Keyword(s):  
Durum Wheat ◽  
Molecular Characterization ◽  
Storage Proteins ◽  
Wheat Varieties

Genetic Diversity and Molecular Characterization of Brazilian Wheat Varieties Obtained by Microsatellite Markers

2015 ◽  
Vol 4 (3) ◽  
pp. 1-10
Author(s):  
André Silva ◽  
Marcelo Oliveira ◽  
Elisa Vieira ◽  
Volmir Marchioro ◽  
Francisco Franco ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Molecular Characterization ◽  
Wheat Varieties

Stray light and ghosts in catadioptric spectrometers: incorporating grating scatter measurements into simulations and ghost sensitivity into system design

2018 ◽  
Vol 7 (6) ◽  
pp. 377-386 ◽  
Author(s):  
Monika Kroneberger ◽  
Andreas Mezger ◽  
Stephanie Becker
Keyword(s):  
High Sensitivity ◽  
Stray Light ◽  
Multiple Diffraction ◽  
Optical Elements ◽  
Optical Instruments ◽  
Light Requirements ◽  
Measurement Results ◽  
Selection Of

Abstract The accurate simulation of stray light is essential for the verification of the contrast requirements in optical instruments. In a spectrometer, the scattering from reflective gratings is difficult to characterize while contributing significantly to the overall system stray light and reduction of the spectrometer contrast. In addition, the multiple diffraction orders create a ghost sensitive environment, which must be considered in the design of the instrument. In this article, we present an experimental setup for, and measurement results from, the characterization of the bidirectional scattering distribution function (BSDF) of a holographic grating for a spectrometer applied in a typical earth observation mission with demanding stray light requirements. We observed distinct stray light peaks out of the diffraction plane, which are called ‘satellites.’ The main challenges in the measurement of grating BSDFs arise from the near angle limit, the determination of the instrument signature and the selection of the appropriate sampling (2D or 3D). Following the grating characterization, the next step is to introduce these measured BSDFs into stray light simulation. We have done that by fitting appropriate functions to the measured BSDF and defining them in the optical analysis software ASAP as a user-defined BSDF. Ghost analysis is done at the spectrometer level as a sensitivity analysis of the tilts of the optical elements. Due to the ghosting of higher diffraction orders of the grating, a high sensitivity to the tilts of some of the optical elements can be seen.

scholarly journals Genetic Variability and Phylogenetic Relationship of Pakistani Snapmelon (Cucumis melo var. Momordica) by Using Microsatellite Markers

2016 ◽  
Vol 8 (7) ◽  
pp. 163
Author(s):  
Ghulam Rasool ◽  
Muhammad J. Jaskani ◽  
Amjad Ullakh ◽  
Rashid Ahmad
Keyword(s):  
Microsatellite Markers ◽  
Cucumis Melo ◽  
Genetic Relationships ◽  
Species Conservation ◽  
Genetic Erosion ◽  
Molecular Data ◽  
Data Matrix ◽  
Principle Coordinate Analysis ◽  
Wild Accessions ◽  
Relationship Of

<p>Among the major cucurbit vegetables, <em>Cucumis melo </em>has one of the highest polymorphic fruit types and botanical varieties. The aim of the present study was to evaluate the genetic diversity among different genotypes of Snapmelon (<em>Cucumis melo</em> var. Momordica) collected from all the four provinces of Pakistan. In this study, 18 microsatellite markers were tested on Snapmelon germplasm which yielded valuable information about the genetic relationships among 40 Snapmelon accessions. The mean PIC value of the markers ranged from 0.3706 to 0.8247. For establishing data matrix, an auto radiogram was visually scored for the presence (1) or absence (0) of polymorphic bands. Assessments of genetic relationship among the genotypes were done by cluster analysis, using POPgen software. The genetic analysis through principle coordinate analysis (PCA) and dendrogram showed that the wild accessions were distinguished from all domesticated accessions collected from various regions of the country. Genetic differentiation among the populations using molecular data indicated the importance of the study area for species conservation, genetic erosion estimation, and exploitation in breeding programs.</p>

scholarly journals A novel assay based on pre-equilibrium titration curves for the determination of enzyme inhibitor binding kinetics

2021 ◽  
Author(s):  
Bernard Noppen ◽  
Anouk Vanbelle ◽  
Alan W. Stitt ◽  
Marc Vanhove
Keyword(s):  
Enzyme Inhibitors ◽  
Enzyme Inhibitor ◽  
Peptide Inhibitors ◽  
Pharmacological Agents ◽  
Enzymatic Assays ◽  
Target Interaction ◽  
Titration Curves ◽  
Selection Of

AbstractSelection of pharmacological agents based on potency measurements performed at equilibrium fail to incorporate the kinetic aspects of the drug–target interaction. Here we describe a method for screening or characterization of enzyme inhibitors that allows the concomitant determination of the equilibrium inhibition constant in unison with rates of complex formation and dissociation. The assay is distinct from conventional enzymatic assays and is based on the analysis of inhibition curves recorded prior to full equilibration of the system. The methodology is illustrated using bicyclic peptide inhibitors of the serine protease plasma kallikrein.

scholarly journals Genetic and Morpho-Agronomic Characterization of Sicilian Tetraploid Wheat Germplasm

2021 ◽  
Author(s):  
Angelo Sicilia ◽  
Umberto Anastasi ◽  
Michele Bizzini ◽  
Stefania Montemagno ◽  
Carmelo Nicotra ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Agronomic Traits ◽  
Tetraploid Wheat ◽  
Germplasm Conservation ◽  
Principal Component ◽  
Wheat Breeding ◽  
Negative Consequences ◽  
Mountain Areas ◽  
Wheat Varieties

Abstract Cereal landraces are a very valuable resource in contemporary agriculture. A renewed focus for breeding purposes could ameliorate some negative consequences of modern agriculture and conventional breeding, such as the loss of genetic diversity. A strategy combining molecular genotyping and characterization of morpho-agronomic traits related to productivity is proposed to assess a group of tetraploid wheat landraces named “Bufala”, historically cultivated in the mountain areas of Sicily and used for the production of a traditional bread type locally appreciated. A total of 55 SSR molecular markers were used to detect pattern of diversity for 30 rivet and durum wheat genotypes. Furthermore, phenotyping was than conducted for 8 morpho-agronomic traits. Discriminant analysis of principal components (DAPC), STRUCTURE and phylogenetical analysis allowed to identify three groups, two of them genetically close and including both “Bufala” and “Bufala-related” rivet landraces. To the third group old and more recent durum wheat varieties, constituting the outgroup, were assigned. Clustering was confirmed by Principal Component Analysis (PCA). Finally, a correlation analysis showed as “Bufala” genotypes are characterized by lower ear density compared with the other studied genotypes, a major ear length and a later earing time. The generated knowledge about the levels of diversity and population structure could be an important contribution for parent selection in tetraploid wheat breeding programs in order to define a plant ideotype suitable for low-input and organic cropping systems, as well as for germplasm conservation and management.

Wavelet Based Separation for Synthetic Topographical Characterization of Surface Prepared by Abrasive Water Jet

2012 ◽  
Vol 229-231 ◽  
pp. 2648-2652 ◽  
Author(s):  
Song Lin Wu ◽  
J. Valicek
Keyword(s):  
Wavelet Decomposition ◽  
Water Jet ◽  
Abrasive Water Jet ◽  
Optical Instrument ◽  
Form Error ◽  
Decomposition Level ◽  
Wavelet Bases ◽  
Selection Of

To study the synthetic topographical characterization of surface created by abrasive water jet (AWJ) and carry out a detailed research for the process of surface creating, this paper researches the surface signals obtained both by contact profiler and a new developed optical instrument. Through wavelet decomposition and re-construction of surface signal, surface features, such as form error, waviness and roughness are separated rationally. Some fundamental issues, such as the selection of wavelet bases and the determination of wavelet decomposition level are discussed in detail.

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