scholarly journals Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

2017 ◽  
Vol 45 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Andrei CONEAC ◽  
Meda Sandra ORASAN ◽  
Daniel Corneliu LEUCUTA ◽  
Nicoleta DECEA ◽  
Miuta FILIP ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Experimental Inflammation ◽  
Group Ii

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

Increased total antioxidant capacity in renal tissue of female BWF1 mice infected with Plasmodium chabaudi

2017 ◽  
Author(s):  
Mostafa A. Abdel- Maksoud ◽  
Fathy A. Abdel- Ghaffar ◽  
Azza El- Amir ◽  
Gamal Badr ◽  
Saleh Al- Quraishy
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Renal Tissue ◽  
Hepatic Tissue ◽  
Control Group ◽  
Plasmodium Chabaudi ◽  
Group Iii ◽  
Group I ◽  
Total Antioxidant ◽  
Urine Biochemistry

We have investigated the effect of malaria infection on the urine biochemistry and the renal and hepatic levels of total antioxidant capacity (TAC) in female BWF1 lupus mice. A total of 30 female BWF1 lupus mice were divided into three groups as follows: group (I) control group; group (II) lupus mice infected with live Plasmodium. chabaudi; group (III) lupus mice infected with irradiated P. chabaudi. Mice were killed at day 14 post-infection and plasma samples were collected. live P. chabaudi infection was associated with decreased level of glucose in urine (glucosuria), increased urinary level of both ketones (ketonuria) and blood (hematuria). Additionally, live P. chabaudi infection was associated with increased level of TAC in the renal tissue with decreased level of TAC in the hepatic tissue of infected mice. In conclusion, P. chabaudi infection has a direct effect on the urine biochemistry and the renal and hepatic levels of TAC in BWF1 mice.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Blood Levels of Oxidant/Antioxidant Parameters in Rats Infected withToxoplasma gondii

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Somayeh Bahrami ◽  
Ali Shahriari ◽  
Mehdi Tavalla ◽  
Somayeh Azadmanesh ◽  
Hossein Hamidinejat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Parasitic Infection ◽  
Chronic Phase ◽  
Antioxidant Defense System ◽  
Female Rats ◽  
Control Group ◽  
Blood Levels ◽  
Total Antioxidant

Toxoplasmosis is a common parasitic infection in the world. Since increased free radicals and oxidative stress are reported in many parasitic diseases the purpose of the present study was to evaluate the oxidative stress in acute and chronic toxoplasmosis. RH strains ofToxoplasmatachyzoites were used in the present study. Twenty-five female rats were infected with the parasite while 25 other rats were as the control group that received normal saline. Zero-, 5-, 7-, 10-, and 45-day postinfection (DPI) blood samples were taken. Some parameters related to oxidant and antioxidants such as antioxidant enzymes, malondialdehyde, and total antioxidant capacity were measured. On day 7 after infection, GPX activity and GSH level were significantly increased and in the mentioned day the amount of total antioxidant capacity was significantly reduced. In other cases, there were no significant differences between the groups in different days. Overall, based on the results it seems that, on day 7 after infection, in infected rats responses to oxidative stress were triggered and led to decrease of total antioxidant capacity. Furthermore, glutathione was increased to cope with stress. It seems that probably antioxidant defense system entered the infection to the chronic phase and changed the parasites stage.

scholarly journals Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Akheruz Zaman Ahmed ◽  
Shakta Mani Satyam ◽  
Prakashchandra Shetty ◽  
Melanie Rose D’Souza
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Methyl Gallate ◽  
Group Iii ◽  
Group I ◽  
Female Wistar Rats ◽  
Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p  < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p  < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

scholarly journals The Association between Diabetic Retinopathy and Levels of Ischemia-Modified Albumin, Total Thiol, Total Antioxidant Capacity, and Total Oxidative Stress in Serum and Aqueous Humor

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kadir Kirboga ◽  
Ayse V. Ozec ◽  
Mustafa Kosker ◽  
Ayhan Dursun ◽  
Mustafa I. Toker ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Antioxidant Capacity ◽  
Aqueous Humor ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Total Thiol ◽  
Median Serum ◽  
Total Antioxidant ◽  
Total Oxidative Stress

Purpose. To investigate the oxidant and antioxidant status of patients with type 2 diabetes mellitus and nonproliferative diabetic retinopathy (DRP).Methods. Forty-four patients who had cataract surgery were enrolled in the study. We included 22 patients with DRP in one group and 22 patients in the control group. Samples of aqueous humor and serum were taken from all patients. Serum and aqueous ischemia-modified albumin (IMA), total thiol, total antioxidant capacity (TAC), and total oxidative stress (TOS) levels were compared in two groups.Results. Median serum IMA levels were 44.80 absorbance units in the DRP group and 40.15 absorbance units in the control group (P=0.031). Median serum total thiol levels in the DRP group were significantly less than those in the control group (3051.13 and 3910.12, resp.,P=0.004). Mean TOS levels in the serum were 2.93 ± 0.19 in the DRP group and 2.61 ± 0.26 in the control group (P=0.039). The differences in mean total thiol, TAC, and TOS levels in the aqueous humor and mean TAC levels in the serum were not statistically significant.Conclusion. IMA, total thiol, and TOS levels in the serum might be useful markers in monitoring the risk of DRP development.

scholarly journals Placental Oxidative Stress in the pathogenesis of Hypertensive Disorders of Pregnancy

2021 ◽  
Vol 1 (2) ◽  
pp. 39-49
Author(s):  
Martin Awe Akilla
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Control Group ◽  
Hypertensive Disorders Of Pregnancy ◽  
Oxidative Stress Index ◽  
Hypertensive Disorders ◽  
Total Antioxidant ◽  
Placental Oxidative Stress

Background: Hypertensive disorders of pregnancy are a major complication of pregnancies and can lead to fetal growth retardation, premature delivery and maternal morbidity and mortality. The study aimed at assessing the potential role of the placenta in the pathogenesis of hypertensive disorders of pregnancy. Methods: This study was a case-control study conducted at the Upper East Regional Hospital, Ghana from September, 2016 to March 2017. Twenty (20) pregnant women with hypertensive disorders of pregnancy (i.e., Pregnancy induced hypertension, preeclampsia and eclampsia) as cases and 30 normotensive pregnancies as controls, were included in the study. The placenta was excised after delivery, homogenized and assayed for malondialdehyde, catalase, total peroxide, oxidative stress index, total antioxidant capacity and placental lipid profile. Results: The ages of the two groups were similar, with malondialdehyde (p = 0.001) and Oxidative Stress Index (p < 0.001) being significantly higher in the hypertensive group compared to the control group whereas Total Antioxidant Capacity (p < 0.001) and Catalase (p = 0.011) were significantly higher in the control group compared to the hypertensive group. The proportion of normal, term and livebirth deliveries were significantly higher among controls compared to the hypertensive disorders of pregnancy group. Among the estimated oxidative stress markers, total antioxidant capacity turned out to be the best predictor of the hypertensive disorders of pregnancy. Conclusion: Our findings suggest oxidative stress in women with hypertensive disorders of pregnancy and that placental oxidative stress could be the driving factor for the pathogenesis and severity of these hypertensive disorders of pregnancy.

scholarly journals Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders

2020 ◽  
Author(s):  
Neda Omidpanah ◽  
Saba Ebrahimi ◽  
Asad Vaisi Raygani ◽  
Hadi Mozafari ◽  
Mansour Rezaei
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Temporomandibular Disorders ◽  
Catalase Activity ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
Healthy Controls ◽  
Oxidative Stress Biomarkers ◽  
Reducing Ability ◽  
Total Antioxidant

Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls. Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30.7±13.2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29.16±11.2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), catalase activity, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test.  Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0.001). But there were no significant differences in catalase (P=0.49) and total antioxidant capacity (P=0.22) of TMD patients and healthy controls. Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.

Evaluation and correlation of oxidative stress and haemato-biochemical observations in horses with natural patent and latent trypanosomosis in Punjab state of India

2018 ◽  
Vol 63 (4) ◽  
pp. 733-743 ◽  
Author(s):  
Rahul Parashar ◽  
Lachhman Das Singla ◽  
Mayank Gupta ◽  
Suresh Kumar Sharma
Keyword(s):  
Oxidative Stress ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Latent Group ◽  
Biochemical Indices ◽  
And Oxidative Stress

Abstract The haemato-biochemical indices and oxidative stress markers in horses naturally infected with Trypanosoma evansi were evaluated by analyzing the level of these parameters between T. evansi infected (microscopically positive patent group and PCR positive latent group) and infection free horses. To compare the hemato-biochemical indices and oxidative stress indicators, horses were divided into three categories based on diagnostic test employed and positive results obtained. These included Romanowsky stained slide positive group (Group I; n = 6), PCR positive group (group II; n = 28) and negative control group (group III, n = 30), revealing parasitologically positive patent, molecular positive latent and disease free status of horses. A significant reductions in total erythrocytes count (TEC, P = 0.01), haemoglobin (Hb, P = 0.01) and packed cell volume (PCV, P = 0.04) was noticed both in group I and group II while significant neutrophilia and lymphocytopenia was observed in group I when compared to negative control group. Substantial increase in creatinine (CRTN, P = 0.032) and gamma glutamyl transferase (GGT, P = 0.012) in group I while significant decrease in glucose (GLU, P = 0.04) and iron (Fe, P = 0.01) were noticed in both group I and group II in comparison to group III. A significant difference in lipid peroxides (LPO, P = 0.01) with highest level in patent group I (15.33 ± 0.53) followed by PCR positive latent group (14.09 ± 1.66) indicates higher lipid peroxidation in erythrocytes and oxidative stress in decreasing order when compared with infection free control horses (9.83 ± 0.97). Catalase (CAT, P = 0.01) was significantly lower in parasitological (0.82 ± 0.14) and molecular positive cases (1.27 ± 0.35) in comparison to control group (3.43 ± 0.96). The levels of superoxide dismutase (SOD, P = 0.01), reduced glutathione (GSH, P = 0.01) and ferric reducing antioxidant power (FRAP, P = 0.01) were significantly lower in parasito-molecular positive cases as compared to infection free control horses. An inverse correlation of RBC count with LPO and GSH and a direct correlation with catalase, SOD and FRAP was revealed. Overall, the observed substantial decreases in the oxidative parameters like catalase CAT, SOD, GSH and FRAP activities with remarkably elevated levels of LPO indicate high exposure of erythrocytes to oxidative damage in T.evansi infected horses.

scholarly journals Investigation of Protective Effects of Quercetin on Oxidative Stress Induced by Vinblastine in Bone Marrow of Rats

2020 ◽  
Vol 27 (2) ◽  
pp. 194-200
Author(s):  
Hamed Aramjoo ◽  
Habibollah Ebrahim Zadeh ◽  
Mina Hemmati
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Single Dose ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Therapeutic Potential ◽  
Blood Analysis ◽  
Control Group ◽  
Chemotherapy Drugs ◽  
Total Antioxidant

Background: Chemotherapy drugs such as vinblastine cause oxidative stress in the bone marrow resulting changes in blood cell production and anemia. In this study, the antioxidant and therapeutic potential of quercetin was evaluated. Methods: Twenty-one male Wistar rats were divided into three groups; The Control group received a daily dose of normal saline, group 2 received a single dose of 2 mg/kg b.w. vinblastine intraperitoneally (i.p.) on the first day of study, and group 3 received a single dose of vinblastine (2mg/kg b.w. i.p.) along with quercetin (20 mg/kg b.w. i.p.) for 14 days. To evaluate oxidative stress in bone marrow; malondialdehyde (MDA), Total Antioxidant Capacity (TAC) and Pro-Oxidant/Antioxidant Balance (PAB) were also measured using specified methods. Results: The blood analysis showed that the mean level of RBC, Hemoglobin, and Hematocritwere significantly higher in the vinblastine group compared to the control group. Treatment with quercetin could elevate them into the normal range. Administration of vinblastine elevated the levels of bone marrow MDA and PAB significantly (p<0.05) compared to the control group but had no effect on total antioxidant capacity. The use of quercetin with vinblastine showed a decrease in the levels of bone marrow MDA and PAB compared to the vinblastine group alone. Conclusion: The findings of this study showed that quercetin at a dose of 20 mg/kg could improve the anemia induced by vinblastine chemotherapy, and it can also be useful in improving vinblastine-induced lipotoxicity.

Evaluation of total oxidative status and total antioxidant capacity in patients with endemic fluorosis

2011 ◽  
Vol 29 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Ercan Varol ◽  
Atilla Icli ◽  
Fatih Aksoy ◽  
Hasan Aydin Bas ◽  
Recep Sutcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Stress Index ◽  
Control Group ◽  
Healthy Controls ◽  
Endemic Fluorosis ◽  
Total Antioxidant ◽  
Total Oxidative Status

The objective of the present study was to determine the plasma total oxidative status (TOS) and total antioxidant capacity (TAC) in patients with endemic fluorosis. A total of 79 (35 males and 44 females; mean age 44.0 ± 11.9 years) patients with endemic fluorosis and 55 (23 males and 32 females; mean age 48.3 ± 8.5 years) age-, sex- and body mass index-matched healthy controls were included in this study. The urine fluoride levels and plasma TOS and TAC levels were measured. The urine fluoride levels of fluorosis patients were significantly higher than control subjects as expected (1.91 ± 0.15 vs. 0.49 ± 0.13 mg/L, respectively; p < 0.001). TOS was significantly higher in fluorosis group than in control group (17.55 ± 3.82 vs. 15.06 ± 4.31 μmol H2O2 Eq/L, respectively; p = 0.001). TAC was significantly lower in fluorosis group than in control group (1.60 ± 0.36 vs. 1.82 ± 0.51 mmol Trolox Eq/L, respectively; p = 0.004). Oxidative stress index (OSI) was significantly higher in fluorosis group than in control group (11.5 ± 3.8 vs. 8.8 ± 3.7, respectively; p < 0.001). Correlation analysis in all the groups indicated that TAC was negatively correlated with urine fluoride ( r = −0.25, p = 0.003), TOS was positively correlated with urine fluoride ( r = 0.34, p < 0.001) and OSI was positively correlated with urine fluoride ( r = 0.36, p < 0.001). The results of our study demonstrate that oxidative stress plays an important role in the pathogenesis of the endemic fluorosis.

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