scholarly journals Assay of Antioxidant Capacity and Phenolic Compounds in some Romanian and Cypriot Wine

2018 ◽  
Vol 46 (1) ◽  
pp. 240-246 ◽  
Author(s):  
Camelia E. LUCHIAN ◽  
Cintia L. COLIBABA ◽  
Maria CODREANU ◽  
Ştefan TUDOSE-SANDU-VILLE ◽  
Marius NICULAUA ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Health ◽  
High Performance ◽  
Relevant Information ◽  
Red Wines ◽  
Health It ◽  
Material Degradation ◽  
Liquid Chromatograph ◽  
Dpph Method

Free radicals have an important role in food and in chemical material degradation, contributing to the occurrence of many human health problems, but the antioxidants can considerably delay or prevent the oxidation of easily oxidable substrates. The present research aimed to assess the antioxidant activity, expressed by the presence of polyphenols, flavonols, flavones, anthocyanidins and flavanols, in several Romanian and Cypriot wines. The wine phenolics content was analysed by high-performance liquid chromatograph (HPLC) Shimadzu equipped with two chromatographic columns. Higher concentrations were registered in all red wines. The antioxidant activity quantification was carried out by the DPPH method, a simple and cheap approach based on the absorbance decrease determination of the DPPH radical (2,2-diphenyl- 1- picrylhydrazyl) in the presence of antioxidants. The highest antioxidant activity for white wines was determined at ‘Spouriko’ for Cypriot wine from 2013 (EC 50 = 1/38) while for Romanian wines, the highest value was found in a ‘Tămâioasă românească’ (EC50 = 1/58) and for red wines at ‘Maratheftiko’ wine from 2012 (EC50 = 1/680) and in ‘Fetească Neagră’ wine from 2014 (EC50 =1/590). This study provides relevant information to consumers and industry alike regarding the beneficial role wine plays for human health. It also can act as a baseline for choosing a certain product, according to its sanogenic potential.

The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

2018 ◽  
Vol 69 (3) ◽  
pp. 627-631 ◽  
Author(s):  
Viorica Ohriac (Popa) ◽  
Diana Cimpoesu ◽  
Adrian Florin Spac ◽  
Paul Nedelea ◽  
Voichita Lazureanu ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Emotional Experience ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Liquid Chromatograph ◽  
Mobile Phase Flow Rate ◽  
Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

2006 ◽  
Vol 830 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Lata Kaphalia ◽  
Bhupendra S. Kaphalia ◽  
Santosh Kumar ◽  
Mary F. Kanz ◽  
Mary Treinen-Moslen
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatograph ◽  
Rat Serum ◽  
Liquid Chromatograph

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

Determination of uric acid in grain using HPLC

2021 ◽  
pp. 74-77
Author(s):  
Артём Валерьевич Яицких ◽  
Геннадий Алексеевич Закладной ◽  
Дмитрий Сергеевич Степаненко
Keyword(s):  
Uric Acid ◽  
Extraction Method ◽  
High Performance ◽  
Reversed Phase ◽  
Calibration Graph ◽  
Improved Method ◽  
Liquid Chromatograph ◽  
Yield Time ◽  
Grain Contamination

В данной статье показана возможность улучшения определения мочевой кислоты методом высокоэффективной жидкостной хроматографии (ВЭЖХ), с помощью увеличения растворимости ее в 1 %-ном растворе ацетата натрия, повышения удержания мочевой кислоты и тем самым изменения времени выхода, что позволяет повысить точность анализа. Проведено сравнение градуировочных растворов и опытных образцов зерна зараженных вредителями хлебных запасов. В процессе исследования был опробован метод для определения и идентификации мочевой кислоты как одного из загрязняющих зерно веществ с помощью ВЭЖХ в обращенной фазе. Экспериментально опробована и усовершенствована методика анализа мочевой кислоты в зерне с использованием жидкостного хроматографа «Стайер». Описаны оборудование и материалы для ВЭЖХ, условия хроматографического разделения и детектирования, построения калибровочного графика, экстракции, включая методику экстракции, сходимости результатов при экстракции и введении экстракта в хроматограф, а также порядок и расчет измерений. Экспериментально показано, что усовершенствованная методика с применением ВЭЖХ позволяет использовать ее для проведения дальнейших исследований зависимости содержания мочевой кислоты от величины загрязнения зерна насекомыми. This article shows the possibility of improving the determination of uric acid by high-performance liquid chromatography (HPLC), by increasing its solubility in a 1 % solution of sodium acetate, increasing the retention of uric acid, and thereby changing the yield time, which allow to improve the accuracy of the analysis, a comparison of calibration solutions and experimental grain samples of pest-infected bread stocks have been carried out. During the research course a method for the determination and identification of uric acid has been tested as one of the grain polluting substance using HPLC in the reversed phase. The method of uric acid in grain analysis using a liquid chromatograph «Stayer» has been experimentally tested and improved. The equipment and materials for HPLC, the conditions of chromatographic separation and detection, the construction of a calibration graph, extraction, including the extraction method, the convergence of the results during extraction and the introduction of the extract into the chromatograph, as well as the course and calculation of measurements have been described. It has been experimentally shown that the improved method with HPLC allows to use it for further research of the uric acid content dependence on the amount of grain contamination by insects.

Determination of 8-methoxypsoralen in suction-blister fluid and serum by liquid chromatography.

1980 ◽  
Vol 26 (13) ◽  
pp. 1825-1828 ◽  
Author(s):  
M J Herfst ◽  
P M Edelbroek ◽  
F A de Wolff
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Blister Fluid ◽  
Liquid Chromatograph ◽  
Suction Blister ◽  
Drug Concentrations ◽  
Suction Blister Fluid

Abstract A method is described for determination of 8-methoxypsoralen to 0.2 mL of suction-blister fluid or 1 mL of serum from psoriatic patients being treated with this drug. The drug is extracted from the biological matrix at pH 9.0 with a mixture of dichloromethane and light petroleum ether. 5-Methoxypsoralen is used as internal standard. Separation and quantitation are performed on a “high-performance” liquid chromatograph with use of an RP 18 reversed-phase column and detection at 245 nm. Accuracy and precision are good. Some benzodiazepines and their metabolites interfere. The lowest detectable concentration is 10 microgram/L, which means that the method is sufficiently sensitive to measure the drug concentrations in serum and suction-blister fluid for pharmacokinetic studies in patients being treated with a therapeutic dosage.

scholarly journals Determination of the Phenolic Profile and Antioxidant Activity of Leaves and Fruits of SpanishQuercus coccifera

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
L. Molina-García ◽  
R. Martínez-Expósito ◽  
M. L. Fernández-de Córdova ◽  
E. J. Llorent-Martínez
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Ion Trap ◽  
Food Samples ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Ion Trap Mass Spectrometer ◽  
High Antioxidant Activity ◽  
Cardioprotective Effects

In this work, we report the phytochemical composition and antioxidant activity of methanol extracts of leaves and fruits (acorns) ofQuercus coccifera(kermes oak). Forty-one compounds were characterized using high-performance liquid chromatography with electrospray multistage mass spectrometry (HPLC-ESI-MSn) with an ion trap mass spectrometer. A high percentage of the detected compounds were gallic acid derivatives, although some saccharides and flavonoids were also present. This phytochemical pattern is typical inQuercusspecies, which are rich in gallotannins. These compounds are partially responsible for the cardioprotective effects observed in different food samples containing them. We evaluated the antioxidant activity by ABTS and DPPH assays. In both cases, high antioxidant activity was observed, being higher in acorns than in leaves. The high antioxidant potential of the extracts, which is related to the high total phenolic content, indicates the potential benefit of the use of this species as a source of bioactive compounds.

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