scholarly journals Role of conventional and molecular techniques in soybean yield and quality improvement: A critical review

2021 ◽  
Vol 49 (4) ◽  
pp. 12555
Author(s):  
Gai YUHONG ◽  
Adnan RASHEED ◽  
Zhao ZHOU ◽  
John J. GARDINER ◽  
Muhammad ILYAS ◽  
...  

The soybean is one of the most significant legume crops around the globe and serves as a source of dietary components for humans and animals. It has a higher percentage of protein compared to any other crop. Soybean yield and quality have been affected by many environmental factors.  The genetic mechanism of yield and quality is still not clearly understood. Hence there is still a need to investigate the major potent factors to shed light on the mechanism behind yield and quality traits in soybean. Recently, a lot of significant work, including novel QTL, genes, and CRISPR-based genome editing in soybeans, has been done, which opened new doors of hope. The current review has presented detailed work done previously. We have also discussed the role of different breeding techniques in the conventional way of soybean improvement. The genetic factors regulating yield, quality, and disease resistance could be further cloned and transferred into elite cultivars to attain higher output in the current situation of changing environment. The integrated use of several techniques, like CRISPR/Cas9, next-generation sequencing, omics approaches, would be a fruitful way to improve soybean yield and quality. Besides this, hybridization, mass selection, pure line selection, backcross breeding, and pedigree selection should be adopted to develop novel soybean cultivars. This review concluded that soybean yield and quality improvement could be enhanced by exploring its genetic mechanism using several molecular and conventional methods.  

2021 ◽  
Vol 49 (4) ◽  
pp. 12605
Author(s):  
Radu E. SESTRAS

The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 4, Volume 49, 2021 represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about: Cotton versus climate change: the case of Greek cotton production; Agronomic and genetic approaches for enhancing tolerance to heat stress in rice; Insights on solanaceous resistance against tomato leafminer (Tuta absoluta), with emphasis on chemical compounds useful in integrated pest management; Role of conventional and molecular techniques in soybean yield and quality improvement - A critical review; Assessment of cold stress tolerance in maize through quantitative trait locus, genome-wide association study and transcriptome analysis; Beneficial microorganisms enhance the growth of basil (Ocimum basilicum L.) under greenhouse conditions; Genome-wide identification and expression profiling of duplicated flavonoid 3'-hydroxylase gene family in Carthamus tinctorius L.; Comparative analysis of genetic diversity in Norway spruce (Picea abies) clonal seed orchards and seed stands, etc.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Selene Rubiola ◽  
Tiziana Civera ◽  
Felice Panebianco ◽  
Davide Vercellino ◽  
Francesco Chiesa

Abstract Background Cattle are intermediate hosts of six Sarcocystis species, among which Sarcocystis hominis and Sarcocystis heydorni can infect humans through the consumption of raw or undercooked meat. In addition to the zoonotic potential, there is increasing interest in these protozoa because of the evidence supporting the role of Sarcocystis spp. in the occurrence of bovine eosinophilic myositis (BEM), a specific inflammatory myopathy which leads to carcass condemnation and considerable economic losses. Actually, all the prevalence studies carried out on cattle in Italy have been based on either morphological or 18S rDNA-based molecular techniques, most likely leading to misidentification of closely related species. Therefore, there is a strong need for new data on the prevalence of the different Sarcocystis spp. in cattle in Italy and their association with bovine eosinophilic myositis. Methods To reach our aim, individual striated muscle samples from BEM condemned carcasses (N = 54) and diaphragm muscle samples from randomly sampled carcasses (N = 59) were obtained from Northwest Italy slaughterhouses. Genomic DNA was extracted and analyzed by multiplex-PCR targeting 18S rDNA and cox1 genes. PCR products amplified using the genus-specific primer set in absence of the specific fragment for S. hirsuta, S. cruzi, S. hominis or S. bovifelis were sequenced to achieve species identification. Results Sarcocystis DNA was detected in 67.8% of the samples from slaughter cattle and in 90.7% of the samples from BEM condemned carcasses. S. cruzi was identified as the most prevalent species in slaughter cattle (61%), followed by S. bovifelis (10.2%), S. hominis (8.5%) and S. hirsuta (1.7%). Notably, among the different Sarcocystis spp. detected, the presence of S. bovifelis and S. hominis was significantly higher in samples isolated from BEM condemned carcasses (46.3% and 40.7% respectively), while there was no statistically significant difference between the presence of S. cruzi or S. hirsuta in BEM condemned carcasses (42.6% and 1.8%, respectively) and randomly sampled carcasses. Furthermore, DNA sequence analysis revealed the presence of a putative new species in two carcasses. Conclusions Our study contributes to updating the data on the prevalence of the different Sarcocystis spp. in cattle in Italy, highlighting the presence of three Sarcocystis spp., S. cruzi, S. hominis and S. bovifelis, in BEM lesions and allowing us to speculate on the possible role of S. hominis and S. bovifelis as the major sarcosporidian species involved in bovine eosinophilic myositis. Graphic Abstract


Land ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 200
Author(s):  
Felicia Chețan ◽  
Cornel Chețan ◽  
Ileana Bogdan ◽  
Adrian Ioan Pop ◽  
Paula Ioana Moraru ◽  
...  

The regional agroecological conditions, specific to the Transylvanian Plain, are favorable to soybean crops, but microclimate changes related to global warming have imposed the need for agrotechnical adaptive measures in order to maintain the level of soybean yield. In this study, we consider the effect of two soil tillage systems, the seeding rate, as well as the fertilizer dosage and time of application on the yield and quality of soybean crops. A multifactorial experiment was carried out through the A × B × C × D − R: 3 × 2 × 3 × 3 − 2 formula, where A represents the year (a1, 2017; a2, 2018; and a3, 2019); B represents the soil tillage system (b1, conventional tillage with mouldboard plough; b2, reduced tillage with chisel cultivator); C represents the fertilizer variants (c1, unfertilized; c2, one single rate of fertilization: 40 kg ha−1 of nitrogen + 40 kg ha−1 of phosphorus; and c3, two rates of fertilization: 40 kg ha−1 of nitrogen + 40 kg ha−1 of phosphorus (at sowing) + 46 kg ha−1 of nitrogen at V3 stage); D represents the seeding rate (1 = 45 germinating grains (gg) m−2; d2 = 55 gg m−2; and d3 = 65 gg m−2); and R represents the replicates (r1 = the first and r2 = the second). Tillage had no effect, the climate specific of the years and fertilization affected the yield and the quality parameters. Regarding the soybean yield, it reacted favorably to a higher seeding rate (55–65 gg m−2) and two rates of fertilization. The qualitative characteristics of soybeans are affected by the fertilization rates applied to the crop, which influence the protein and fiber content in the soybean grains. Higher values of protein content were recorded with a reduced tillage system, i.e., 38.90 g kg−1 DM in the variant with one single rate of fertilization at a seeding rate of 45 gg per m−2 and 38.72 g kg−1 DM in the variant with two fertilizations at a seeding rate of 65 gg m−2.


2021 ◽  
Vol 69 (2) ◽  
pp. 484-499
Author(s):  
Helen Traill

The question of what community comes to mean has taken on increasing significance in sociological debates and beyond, as an increasingly politicised term and the focus of new theorisations. In this context, it is increasingly necessary to ask what is meant when community is invoked. Building on recent work that positions community as a practice and an ever-present facet of human sociality, this article argues that it is necessary to consider the powerful work that community as an idea does in shaping everyday communal practices, through designating collective space and creating behavioural expectations. To do so, the article draws on participant observation and interviews from a community gardening site in Glasgow that was part of a broader research project investigating the everyday life of communality within growing spaces. This demonstrates the successes but also the difficulties of carving out communal space, and the work done by community organisations to enact it. The article draws on contemporary community theory, but also on ideas from Davina Cooper about the role of ideation in social life. It argues for a conceptual approach to communality that does not situate it as a social form or seek it in everyday practice, but instead considers the vacillation between the ideation and practices of community: illustrated here in a designated community place. In so doing, this approach calls into focus the frictions and boundaries produced in that process, and questions the limits of organisational inclusivity.


Author(s):  
Tony Badrick ◽  
Mohamed Saleem ◽  
Wesley Wong

Background Reporting critical results in a timely manner is a crucial role of clinical laboratories. Traditionally, these results were reported using the phone or fax system. However, there are now other modes of communication for this reporting. Quality improvement in any organization is driven by detection of errors and benchmarking against peers. In the case of critical result reporting, there are few current widely used Benchmarking schemes. Methods The Roche Clinical Chemistry Benchmarking Survey in 2019 added questions about critical result reporting including the mode of communication and turnaround time key performance index. This survey includes over 1100 laboratories from 20 countries. Results The survey revealed a range of communication strategies with phone calls still the commonest followed by email. The key performance index for most laboratories was less than 10 min. Conclusion Benchmarking can provide key information for quality improvement activities, particularly pre- and postanalytical.


2019 ◽  
Vol 22 (4) ◽  
pp. 744-752
Author(s):  
Sisira Dharmasri Jayasekara ◽  
Iroshini Abeysekara

Purpose The purpose of this paper is to discuss the role of digital forensics in an evolving environment of cyber laws giving attention to Bay of Bengal Initiative for Multi-Sectoral Technical and Economic Cooperation (BIMSTEC) countries, comprising Bangladesh, India, Myanmar, Sri Lanka, Thailand, Nepal and Bhutan, in a dynamic global context. Design/methodology/approach This study uses a case study approach to discuss the digital forensics and cyber laws of BIMSTEC countries. The objective of the study was expected to be achieved by referring to decided cases in different jurisdictions. Cyber laws of BIMSTEC countries were studied for the purpose of this study. Findings The analysis revealed that BIMSTEC countries are required to amend legislation to support the growth of information technology. Most of the legislation are 10-15 years old and have not been amended to resolve issues on cyber jurisdictions. Research limitations/implications This study was limited to the members of the BIMSTEC. Originality/value This paper is an original work done by the authors who have discussed the issues of conducting investigations with respect to digital crimes in a rapidly changing environment of information technology and deficient legal frameworks.


2016 ◽  
Vol 31 (4) ◽  
pp. 367-372 ◽  
Author(s):  
Lianne Jeffs ◽  
Alyssa Indar ◽  
Britt Harvey ◽  
Julie McShane ◽  
Sue Bookey-Bassett ◽  
...  
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