scholarly journals Cucumber mosaic virus resistance and reproductive biology of brazilian melon accessions

2019 ◽  
Vol 41 (5) ◽  
Author(s):  
Nadia Carolina Sanabria-Verón ◽  
Cláusio Antônio Ferreira de Melo ◽  
Jadergudson Pereira ◽  
Glauber Henrique de Sousa Nunes ◽  
Ohana Luiza Santos de Oliveira ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Reproductive Biology ◽  
Pollen Viability ◽  
Breeding Population ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Reproductive Characteristics ◽  
Quantitative Expression ◽  
Cmv Resistance

Abstract In melon (Cucumis melo) the resistance to Cucumber Mosaic Virus (CMV) and the knowledge about reproductive characteristics are important for selection of genitors for breeding population. So, the objective was to investigate if different accessions of melon from a Brazilian germplasm bank have variability in resistance to CMV (FNY strain) and polymorphism in resistance loci markers, as well as whether there is genetic diversity on resistance and floral morphology descriptors and reproductive biology, aiming at genetic improvement. The symptoms induced by CMV were evaluated in 52 melon genotypes which were genotyped with three markers of single nucleotide polymorphisms related to CMV resistance. A sample of 24 genotypes was characterized for floral and reproductive descriptors. The significant variation in resistance levels allows classifying the accessions in five groups. The polymorphisms detected by the markers were independently distributed in these groups, consistent with the oligogenic quantitative expression of the melon’s resistance. There were significant differences between accessions in floral and reproductive descriptors. Through the Ward-MLM multivariate strategy, these accesses were distributed into seven groups with distinct reproductive characteristics, including variability in pollen viability. Therefore, there are Brazilian accessions of melon that have resistance to CMV, as well as variability in reproductive characteristics.

scholarly journals Construction of a High-density Genetic Linkage Map and QTL Analysis of Resistance to Cucumber Mosaic Virus in Luffa cylindrica (L.) Roem. Based on Specific Length Amplified Fragment Sequencing

2019 ◽  
Author(s):  
Lina Lou ◽  
Zhe Liu ◽  
Xiaohong Liu ◽  
Xiaojun Su
Keyword(s):  
Linkage Map ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Average Distance ◽  
High Density ◽  
Chromosome 1 ◽  
Pcr Analysis ◽  
Nucleotide Polymorphisms ◽  
Luffa Cylindrica ◽  
Cmv Resistance

Abstract Background: Luffa cylindrica L. is an economically important vegetable crop that is consumed globally. Cucumber mosaic virus (CMV) is an important virus affecting Luffa spp. No specific high-density maps have been constructed owing to a lack of efficient markers. Furthermore, no genes or quantitative trait loci (QTLs) are reportedly responsible for CMV resistance in Luffa spp. The development of next-generation sequencing has enabled discovery of single nucleotide polymorphisms and high-throughput genotyping of large populations. Results: A total of 271.01 Mb pair-end reads were generated. The average sequencing depth was 86.19× in both maternal and parental lines, and 14.57× in each F 2 individual. When filtering low-depth specific locus amplified fragment (SLAF) tags, 100,077 high-quality SLAFs were detected, and 7,405 of them were polymorphic. Finally, 3,701 of the polymorphic markers were selected for genetic map construction, and 13 linkage groups were generated. The map spanned 1,518.56 cM with an average distance of 0.41 cM between adjacent markers. Our results also revealed that CMV resistance was regulated by QTLs. Based on the newly constructed high-density map, two loci located on chromosome 1 (100.072 ~ 100.457 cM) and 4 (42.475 ~ 44.398 cM) were identified to regulate CMV resistance in L. cylindrica . A gag-polypeptide of LTR copia-type retrotransposon was predicted as the candidate gene responsible for CMV resistance in L. cylindrica . Conclusions: A high-density linkage map of L. cylindrica was constructed using SLAF. QTL mapping based on CMV disease phenotypes of F 2 led to the identification of two QTL on chromosome 1 and 4, respectively. Kompetitive allele-specific PCR analysis of 60 F 2 individuals, which gave rise to F 2:3 individuals, was carried out. We found that the QTL on chromosome 1 was associated with CMV resistance. Mapping of CMV QTL combined with the transcriptomic sequence alignment identified a gag-polypeptide of LTR copia-type retrotransposon as the most likely causal gene.

scholarly journals Construction of a High-density Genetic Linkage Map and QTL Analysis of Resistance to Cucumber Mosaic Virus in Luffa cylindrica (L.) Roem. Based on Specific Length Amplified Fragment Sequencing

2019 ◽  
Author(s):  
Lina Lou ◽  
Zhe Liu ◽  
Xiaohong Liu ◽  
Xiaojun Su
Keyword(s):  
Linkage Map ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Average Distance ◽  
High Density ◽  
Chromosome 1 ◽  
Pcr Analysis ◽  
Nucleotide Polymorphisms ◽  
Luffa Cylindrica ◽  
Cmv Resistance

Abstract Background: Luffa cylindrica L. is an economically important vegetable crop that is consumed globally. Cucumber mosaic virus (CMV) is an important virus affecting Luffa spp. No specific high-density maps have been constructed owing to a lack of efficient markers. Furthermore, no genes or quantitative trait loci (QTLs) are reportedly responsible for CMV resistance in Luffa spp. The development of next-generation sequencing has enabled discovery of single nucleotide polymorphisms and high-throughput genotyping of large populations. Results: A total of 271.01 Mb pair-end reads were generated. The average sequencing depth was 86.19× in both maternal and parental lines, and 14.57× in each F 2 individual. When filtering low-depth specific locus amplified fragment (SLAF) tags, 100,077 high-quality SLAFs were detected, and 7,405 of them were polymorphic. Finally, 3,701 of the polymorphic markers were selected for genetic map construction, and 13 linkage groups were generated. The map spanned 1,518.56 cM with an average distance of 0.41 cM between adjacent markers. Our results also revealed that CMV resistance was regulated by QTLs. Based on the newly constructed high-density map, two loci located on chromosome 1 (100.072 ~ 100.457 cM) and 4 (42.475 ~ 44.398 cM) were identified to regulate CMV resistance in L. cylindrica . A gag-polypeptide of LTR copia-type retrotransposon was predicted as the candidate gene responsible for CMV resistance in L. cylindrica . Conclusions: A high-density linkage map of L. cylindrica was constructed using SLAF. QTL mapping based on CMV disease phenotypes of F 2 led to the identification of two QTL on chromosome 1 and 4, respectively. Kompetitive allele-specific PCR analysis of 60 F 2 individuals, which gave rise to F 2:3 individuals, was carried out. We found that the QTL on chromosome 1 was associated with CMV resistance. Mapping of CMV QTL combined with the transcriptomic sequence alignment identified a gag-polypeptide of LTR copia-type retrotransposon as the most likely causal gene.

scholarly journals Breeding Snap Beans for Cucumber Mosaic Virus (CMV) Resistance

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 869C-869 ◽  
Author(s):  
Phillip Griffiths*
Keyword(s):  
Common Bean ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Core Collection ◽  
Interspecific Crosses ◽  
Dry Bean ◽  
Snap Bean ◽  
The Core ◽  
Foliar Symptoms ◽  
Cmv Resistance

Cucumber mosaic virus (CMV) is an aphid-transmitted virus that infects snap bean growing regions in New York State and Wisconsin. The core collection of common bean accessions (Phaseolus vulgaris), the complete collection of scarlet runner bean accessions (Phaseolus coccineus) and snap/dry bean cultivars were screened for resistance to CMV. Although variation in foliar symptom expression was observed, no resistance was observed in 93 snap bean and16 dry bean cultivars tested, and only one of the 406 accessions from the core collection (PI 309881) was symptomless. PI 309881 did not have common bean characteristics, and was later identified as a tepary bean (Phaseolus acutifolius) accession based on morphology and PCR-RFLP of chloroplast DNA. Screening of 260 P. coccineus accessions was inaccurate when a visual rating of foliar symptoms was used. It was necessary to determine infection using ELISA and test plant screening with grey zuccini. Using this approach it was determined that 80 P. coccineus accessions were susceptible to CMV; however, the remaining accessions provided possible sources for transfer of CMV resistance to snap bean. Crosses of P. coccineus accessions were made to breeding line 5-593 and backcrossed to 5-593 and snap bean cultivar `Hystyle'. PI 309881 was crossed with ICA Pijao in order to develop interspecific hybrids. Populations were developed from the interspecific crosses/backcrosses and evaluated for CMV resistance using ELISA and visual ratings of foliar symptoms.

scholarly journals Pepper Crop Improvement Against Cucumber Mosaic Virus (CMV): A Review

2020 ◽  
Vol 11 ◽  
Author(s):  
Ning Li ◽  
Chuying Yu ◽  
Yanxu Yin ◽  
Shenghua Gao ◽  
Fei Wang ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Cultural Practices ◽  
Crop Improvement ◽  
Resistance Breeding ◽  
Control Measures ◽  
Sources Of Resistance ◽  
Breeding Lines ◽  
Novel Technologies ◽  
Cmv Resistance

Cucumber mosaic virus (CMV) is a prevalent virus affecting the quality and yield of pepper, resulting in yield losses of greater than 80% during severe local epidemics. Cultural practices and the heavy use of agrochemicals are the most common control measures for CMV. Sources of resistance provide a practical reference and a basis for breeding for CMV resistance. Genetic factors underlying CMV resistance have been studied and advanced breeding lines and cultivars with improved resistance have been developed by traditional breeding methods. Additionally, QTLs or genes for CMV resistance have been identified and can be utilized for marker-assisted resistance breeding. This review focuses on status and prospect of CMV against different virus strains, host resistance, and its applied genetics. With the advent of novel technologies, more useful markers and precise approaches can facilitate the progress for improving CMV resistance in Capsicum.

scholarly journals Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Lara Pretorius ◽  
Richard L. Moyle ◽  
Jessica Dalton-Morgan ◽  
Nasser Hussein ◽  
Peer M. Schenk
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Sanger Sequencing ◽  
Turnip Mosaic Virus ◽  
Australian Isolate ◽  
Nucleotide Polymorphisms ◽  
Mechanical Inoculation ◽  
Rna Seq ◽  
Single Nucleotide ◽  
Before And After

The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10.

scholarly journals Phenotypic and Marker-assisted Breeding of Capsicum for Cucumber Mosaic Virus Resistance

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 595c-595 ◽  
Author(s):  
R.C. Grube ◽  
Y. Zhang ◽  
B. Huang ◽  
M.M. Kyle
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Adult Plant ◽  
Sources Of Resistance ◽  
Seedling Resistance ◽  
Disease Symptoms ◽  
Screening Protocol ◽  
Cmv Resistance ◽  
Selection For ◽  
Different Sources

Resistance to cucumber mosaic virus (CMV) in Capsicum from two sources is being transferred into three commercial types (bell, jalapeno, and Anaheim) using a backcross breeding scheme. We have optimized our CMV seedling screening protocol, which involves multiple inoculations beginning at the cotyledon stage with a severe CMV serogroup I isolate. Both sources of resistance, C. annuum `French Perennial' and a C. frutescens accession (BG2814-6), exhibit oligogenic recessive inheritance and share some but not all resistance alleles. Selection for type in the BCF1 generation had no effect on the frequency of resistant individuals in the BCF2 generation. We have determined that it is necessary to self-pollinate every other backcross generation to screen for resistance. Occasionally disease symptoms appear in adult plants that were initially resistant to multiple inoculations at the seedling stage, and we are investigating the correlation between seedling resistance and adult plant resistance. We are also exploring the extent to which the different sources of resistance behave differently as a function of genetic background. Additionally, we are mapping quantitative trait loci (QTLs) for CMV resistance in pepper with the goal of converting RFLP and/or RAPD markers into PCR-based markers to facilitate molecular marker-assisted selection for CMV resistance.

scholarly journals RFLP TAGGING OF CUCUMBER MOSAIC VIRUS RESISTANCE GENES IN PEPPER VIA DISTRIBUTIONAL EXTREME ANALYSIS

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 914E-914
Author(s):  
Yiping Zhang ◽  
Vince Lackney ◽  
Molly Kyle
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Resistance Breeding ◽  
Linkage Maps ◽  
Recessive Resistance ◽  
Breeding Programs ◽  
Hybridization Probes ◽  
Cmv Resistance ◽  
Genomic Cdna

We report the detection of molecular markers linked to polygenic recessive resistance to cucumber mosaic virus (CMV) in pepper using distributional extreme analysis. A total of 132 mapped tomato genomic, cDNA, and pepper genomic clones from different linkage groups in two existing pepper maps, especially from the ones showing tentative CMVR QTLs, were selected as hybridization probes for Southern blots, in which DNA from the 12 most resistant and 15 most susceptible individuals from a large segregating F2 were probed. Several clones appeared to cosegregate with CMV resistance phenotype. Further analysis is being done to place these markers on existing molecular linkage maps. The precise locations of resistance genes can be defined by examining additional markers within the region. The availability of closely linked DNA markers may facilitate marker-aided selection in pepper CMV resistance breeding programs.

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