Instrument Design May Influence Bacterial Reduction During Root Canal Preparation

Abstract The aim of this study was to evaluate the bacterial reduction promoted by ProTaper Next and Twisted File by comparing to ProTaper Universal and manual technique. Sixty distobuccal root canals of maxillary molars sterilized with ethylene oxide were contaminated with Enterococcus faecalis broth culture. After incubation for 21 days, bacterial samples were collected and cultured on m-Enterococcus agar plates. The root canals were divided into 4 groups, according to the system used for instrumentation: ProTaper Next, Twisted File, ProTaper Universal, and crown down manual technique. Other 8 uncontaminated root canals were control asepsis. Bacterial samples were collected immediately and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by paired t-test and intergroup analysis by ANOVA and Tukey tests, all at 5% significance. All techniques significantly reduced the bacterial number in the root canal (p<0.05). ProTaper Next and Twisted File resulted in more bacterial reduction than ProTaper Universal and manual technique (p<0.05). ProTaper Next and Twisted File were similar (p>0.05). It can be concluded that ProTaper Next and Twisted File promote a higher bacterial reduction than Protaper Universal and manual technique.

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Manual and rotary instrumentation techniques for root canal preparation in primary molars

Dentistry 3000 ◽

10.5195/d3000.2014.19 ◽

2014 ◽

Vol 2 (1) ◽

Author(s):

Francinne M. Rosa ◽

Adriana Modesto ◽

Italo M. Faraco-Junior

Keyword(s):

Root Canal ◽

Pediatric Dentistry ◽

Primary Molars ◽

Second Primary ◽

Root Canal Preparation ◽

Time Saving ◽

Root Canals ◽

Maxillary Molars ◽

Mean Time ◽

Apical Displacement

Introduction: Although rotary instrumentation has been widely studied in permanent dentition, it is a rather new field of study concerning primary teeth. Purpose: We aimed to evaluate apical displacement and time needed for instrumentation of root canals of primary molars by manual and rotary techniques. Materials and Methods: Root canals of 144 extracted first and second primary maxillary molars were randomly divided into 2 groups: I- manual instrumentation (K-files); II- rotary instrumentation (K3 Rotary System®). The canals were radiographed with pathfinding files in place, prepared by both techniques, and instrumentation time was recorded. After preparation, root canals were radiographed again with pathfinding files in place. To analyze the degree of apical displacement, digital images were superimposed using the Adobe Photoshop® software. Results: Mean apical displacement (0.70 mm) in the manual instrumentation group was not statistically different from that in the rotary instrumentation group (0.79 mm). However, mean time for root canal preparation was significantly shorter using the rotary system (128.0 s) than using the manual system (174.0 s) (p<0.05). Conclusions: The use of rotary instrumentation in pediatric dentistry is feasible, offering time-saving advantages in root canal preparation.

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The ability of Reciproc instruments to reach full working length without glide path preparation: a clinical retrospective study

PeerJ ◽

10.7717/peerj.3583 ◽

2017 ◽

Vol 5 ◽

pp. e3583 ◽

Cited By ~ 9

Author(s):

Andreas Bartols ◽

Bernt-Peter Robra ◽

Winfried Walther

Keyword(s):

Retrospective Study ◽

Root Canal ◽

Primary Root ◽

Primary Treatment ◽

Endodontic Treatment ◽

Binary Logistic Regression Model ◽

Root Canals ◽

Maxillary Molars ◽

Glide Path ◽

Working Length

Background Reciproc instruments are the only contemporary root canal instruments where glide path preparation is no longer strictly demanded by the manufacturer. As the complete preparation of root canals is associated with success in endodontic treatment we wanted to assess the ability and find predictors for Reciproc instruments to reach full working length (RFWL) in root canals of maxillary molars in primary root canal treatment (1°RCTx) and retreatment (2°RCTx) cases. Methods This retrospective study evaluated 255 endodontic treatment cases of maxillary molars. 180 were 1°RCTx and 75 2°RCTx. All root canals were prepared with Reciproc instruments. The groups were compared and in a binary logistic regression model predictors for RFWL were evaluated. Results A total of 926 root canals were treated with Reciproc without glide path preparation. This was possible in 885 canals (95.6%). In 1°RCTx cases 625 of 649 (96.3%) canals were RFWL and in 2°RCTx cases 260 of 277 (93.9%). In second and third mesiobuccal canals (MB2/3) 90 out of 101 (89.1%) were RFWL with Reciproc in 1°RCTx and in the 2°RCTx treatment group 49 out of 51 cases (96.1%). In mesio-buccal (MB1) canals “2°RCTx” was identified as negative predictor for RFWL (OR 0.24 (CI [0.08–0.77])). In MB2/3 canals full working length was reached less often (OR 0.04 (CI [0.01–0.31])) if the tooth was constricted and more often if MB2/3 and MB1 canals were convergent (OR 4.60 (CI [1.07–19.61])). Discussion Using Reciproc instruments, the vast majority of root canals in primary treatment and retreatment cases can be prepared without glide path preparation.

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A short review on the root canal configuration of primary maxillary molars

Bioinformation ◽

10.6026/973206300161033 ◽

2020 ◽

Vol 16 (12) ◽

pp. 1033-1036

Author(s):

Deepa Gurunathan ◽

Keyword(s):

Root Canal ◽

Short Review ◽

Type I ◽

Cochrane Central Register ◽

Population Number ◽

Second Molar ◽

Root Canals ◽

Maxillary Molars ◽

Root Canal Morphology ◽

Canal Morphology

It is of interest to compile available information on the root canal morphology of primary maxillary molars from known literature. The literature resources used to collect data include Medline/PubMed, The Cochrane Central Register of Clinical Trials, SIGLE and Science Direct. Data consists of type of population, number of teeth per study, number of root canals, canal length and type of root canal configuration. We used data from a total of 13 studies (951 primary maxillary molars). Maxillary molars (1st and 2nd) are dominant for two roots variant. The first molar the mean root length ranges from 7.9mm – 8.1mm. The second molar ranges from 7.2mm-8.5mm. Type I (explain in a phrase) canal morphology is the common variant in both the molars. Data shows that Root Canal morphology shows variations with the diagnostic aid (example micro CT) used and in different ethnic populations.

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Force and vibration analysis in biomechanical preparation of root canals using reciprocating endodontic file system: In vitro study

Proceedings of the Institution of Mechanical Engineers Part H Journal of Engineering in Medicine ◽

10.1177/09544119211044236 ◽

2021 ◽

pp. 095441192110442

Author(s):

Vinod Singh Thakur ◽

Pavan Kumar Kankar ◽

Anand Parey ◽

Arpit Jain ◽

Prashant Kumar Jain

Keyword(s):

Root Canal ◽

Vibration Analysis ◽

File System ◽

In Vitro Study ◽

Root Canal Preparation ◽

Root Canals ◽

Improper Use ◽

Four Levels ◽

Root Canal Instrumentation

The shaping and cleaning of the root canal are very important in root canal treatment. The excessive force and vibration during biomechanical preparation of the root canal may result in failure of the endodontic file. In this study, force and vibration analysis was carried out during root canal preparation. The samples of human extracted (premolar) teeth were provided by the College of Dental Science and Hospital. Endodontic instruments for reciprocating motion, such as the WaveOne Gold file system, had been used for root canal preparation. Force and vibration signals were recorded by dynamometer and accelerometer, respectively. The acquired signals were denoised using the db4 (SWT denoising 1-D) wavelet. Four levels of decomposition were carried out for each signal. The signal denoising technique was used to remove unwanted noise from the acquired signal. FESEM analysis was used to visualize the levels of severity of endodontic files during the cleaning and shaping of the root canal. In most of the cases, the failure occurred due to the improper use of the root canal instrumentation. The optimum amount of force was used to avoid the file failure and provided the proper instrumentation. The curve fitting regression model was used to find the interdependency between force and vibration.

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Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis

Journal of Clinical Medicine ◽

10.3390/jcm9030854 ◽

2020 ◽

Vol 9 (3) ◽

pp. 854 ◽

Cited By ~ 7

Author(s):

Alexandre P. L. Carvalho ◽

Laura C. L. Nardello ◽

Fernanda S. Fernandes ◽

Fernanda P. Bruno ◽

Luiza R. Paz ◽

...

Keyword(s):

Calcium Hydroxide ◽

Root Canal ◽

Quantitative Polymerase Chain Reaction ◽

Apical Periodontitis ◽

Universal Primers ◽

Rrna Gene ◽

Root Canal Preparation ◽

Root Canals ◽

Mechanical Preparation ◽

Root Canal Disinfection

New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection.

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The impact of minimally invasive root canal preparation strategies on the ability to shape root canals of mandibular molars

International Endodontic Journal ◽

10.1111/iej.13384 ◽

2020 ◽

Vol 53 (12) ◽

pp. 1680-1688

Author(s):

C. O. Lima ◽

A. F. A. Barbosa ◽

C. M. Ferreira ◽

C. M. Augusto ◽

L. M. Sassone ◽

...

Keyword(s):

Minimally Invasive ◽

Root Canal ◽

Root Canal Preparation ◽

Root Canals ◽

Mandibular Molars ◽

The Impact

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Evaluation Algorithm of Root Canal Shape Based on Steklov Spectrum Analysis

Computational and Mathematical Methods in Medicine ◽

10.1155/2019/4830914 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16

Author(s):

Dongqing Wu ◽

Jian Gao ◽

Xiaoli Hu ◽

Zhengtao Xiao ◽

Zhuwei Huang ◽

...

Keyword(s):

Root Canal ◽

Evaluation Method ◽

Complete Removal ◽

Root Canal Preparation ◽

Root Canals ◽

Need To Evaluate ◽

Before And After ◽

Evaluation Algorithm ◽

Steklov Spectrum ◽

Volume Difference

In recent years, we have seen more and more interest in the field of medical images and shape comparison motivated by the latest advances in microcomputed tomography (μCT) acquisition, modelling, and visualization technologies. Usually, biologists need to evaluate the effect of different root canal preparation systems. Current root canal preparation evaluation methods are based on the volume difference, area difference, and transportation of two root canals before and after treatment. The purpose of root canal preparation is to minimize the volume difference and ensure the complete removal of the smear layer. Previous methods can reflect some general geometric differences, but they are not enough to evaluate the quality of root canal shape. To solve this problem, we proposed a novel root canal evaluation method based on spectrum and eigenfunctions of Steklov operators, which can be served as a better alternative to current methods in root canal preparation evaluation. Firstly, the ideal root canal model was simulated according to the root canal model before and after preparation. Secondly, the Steklov spectrum of the two models was calculated. Thirdly, based on the spectrum and the histogram of the Gaussian curvature on the surface, the weight of each eigenvalue was computed. Therefore, the Steklov spectrum distance (SSD), which measures shape difference between the root canals, was defined. Finally, the calculation method that quantifies the root canal preparation effect of root canals was obtained. Through experiments, our method manifested high robustness and accuracy compared with existing state-of-the-art approaches. It also demonstrates the significance of our algorithm’s advantages on a variety of challenging root canals through result comparison with counterpart methods.

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Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

Brazilian Dental Journal ◽

10.1590/0103-6440201300049 ◽

2014 ◽

Vol 25 (5) ◽

pp. 416-419 ◽

Cited By ~ 12

Author(s):

Marcos Rodolfo Bolfoni ◽

Marcelo dos Santos Ferla ◽

Otávio da Silva Sposito ◽

Luciano Giardino ◽

Rogério de Castilho Jacinto ◽

...

Keyword(s):

Antimicrobial Activity ◽

Sodium Hypochlorite ◽

Root Canal ◽

Root Canal Preparation ◽

Human Teeth ◽

Significance Level ◽

Root Canals ◽

Tukey Test ◽

Irrigation Solution ◽

Period Data

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

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