scholarly journals Evaluation of biobutanol production by Clostridium beijerinckii NRRL B-592 using sweet sorghum as carbon source

2015 ◽  
Vol 45 (9) ◽  
pp. 1707-1712 ◽  
Author(s):  
Luiz Jardel Visioli ◽  
Eliana Albornoz Alves ◽  
Aline Trindade ◽  
Raquel Cristine Kuhn ◽  
Marcio Schwaab ◽  
...  
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Sweet Sorghum ◽  
Low Cost ◽  
Inoculum Size ◽  
Clostridium Beijerinckii ◽  
Initial Inoculum ◽  
Sweet Sorghum Juice ◽  
Operational Variables ◽  
Initial Ph

<p>In this research it was evaluated the production of biobutanol by<bold> Clostridium beijerinckii</bold>NRRL B-592 using sweet sorghum juice as carbon source. Operational variables, like pH and initial inoculum size, as well as supplementation of industrial media with yeast extract and tryptone, were evaluated. The maximum butanol obtained was 2.12g kg<sup>-1</sup> using 12.5% of inoculum size, 0.05g 100mL<sup>-1</sup> of tryptone and 0.1g 100mL<sup>-1</sup> of yeast extract and initial pH of 5.5. The main contribution of this research was to show a systematic procedure for development of a low cost industrial media for biobutanol production from sweet sorghum.</p>

Improvement of butanol production from sweet sorghum juice by Clostridium beijerinckii using an orthogonal array design

2016 ◽  
Vol 79 ◽  
pp. 287-294 ◽  
Author(s):  
Likit Sirisantimethakom ◽  
Lakkana Laopaiboon ◽  
Patthranit Sanchanda ◽  
Jetnipit Chatleudmongkol ◽  
Pattana Laopaiboon
Keyword(s):  
Orthogonal Array ◽  
Sweet Sorghum ◽  
Clostridium Beijerinckii ◽  
Butanol Production ◽  
Orthogonal Array Design ◽  
Array Design ◽  
Sweet Sorghum Juice

Validation of Fermentative Parameters for Efficient Succinate Production in Batch Operation by Actinobacillus succinogenes 130ZT

2012 ◽  
Vol 550-553 ◽  
pp. 1448-1454
Author(s):  
Apichai Sawisit ◽  
Supaluk Seesan ◽  
Sitha Chan ◽  
Sunthorn Kanchanatawee ◽  
Sirima Suvarnakuta Jantama ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Actinobacillus Succinogenes ◽  
Brewer’S Yeast ◽  
Succinate Production ◽  
Brewer's Yeast ◽  
Lactose Fermentation ◽  
Initial Ph ◽  
The Cost

Succinate is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present study, the effects of different carbon and nitrogen sources, initial pH of the growth medium (pH 4.5-9.0), and temperature (25-45°C) on the fermentative succinate production by Actinobacillus succinogenes 130ZT were investigated in 100 mL anaerobic bottles. The results revealed that the highest concentration of succinate at 6.28 g/L was produced from 10 g/L of glucose or lactose in the medium containing 5 g/L yeast extract at 24 h. However, a comparable concentration of succinate was also produced when the medium was supplemented with 5 g/L spent brewer’s yeast extract. Based on these results, the cost effectiveness of succinate production could be improved by the use of glucose or lactose fermentation supplemented with spent brewer’s yeast extract. Optimized initial pH at 8.0, temperature at 37 °C, and inoculum size at 6% (v/v) provided the best succinate production at the concentration of 6.37 g/L with a yield of 68.73%.

scholarly journals Improvement of Bioethanol Production from Sweet Sorghum Juice under Very High Gravity Fermentation: Effect of Nitrogen, Osmoprotectant, and Aeration

Energies ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3620 ◽  
Author(s):  
Niphaphat Phukoetphim ◽  
Pachaya Chan-u-tit ◽  
Pattana Laopaiboon ◽  
Lakkana Laopaiboon
Keyword(s):  
Ethanol Production ◽  
Yeast Extract ◽  
Sweet Sorghum ◽  
Control Experiment ◽  
Nutrient Concentrations ◽  
High Gravity ◽  
Very High Gravity ◽  
Sweet Sorghum Juice ◽  
Very High Gravity Fermentation ◽  
Very High

To improve ethanol production fermentation efficiency from sweet sorghum juice under a very high gravity (VHG, 280 g/L of total sugar) condition by Saccharomyces cerevisiae NP01, dried spent yeast (DSY), yeast extract, and glycine concentrations were optimized using an L9 (34) orthogonal array design. The results showed that the order of influence on the ethanol concentration (PE) was yeast extract > glycine > DSY. The optimal nutrient concentrations for ethanol production were determined as follows: yeast extract, 3; DSY, 4; and glycine, 5 g/L. When a verification experiment under the projected optimal conditions was done, the P, ethanol yield (Yp/s), and ethanol productivity (Qp) values were 120.1 g/L, 0.47, and 2.50 g/L·h, respectively. These values were similar to those of the positive control experiment with yeast extract supplementation at 9 g/L. The yeast viability under the optimal condition was higher than that of the control experiment. To improve sugar utilization and ethanol production, aeration at 2.5 vvm for 4 h was applied under the optimal nutrient supplementation. The P, Yp/s, and Qp values were significantly increased to 134.3 g/L, 0.50, and 2.80 g/L·h, respectively.

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Baskar Gurunathan ◽  
Renganathan Sahadevan
Keyword(s):  
Carbon Source ◽  
Aspergillus Terreus ◽  
Inoculum Size ◽  
Soya Bean ◽  
Operating Conditions ◽  
Agitation Rate ◽  
Groundnut Oil ◽  
Initial Ph ◽  
Bean Meal ◽  
Soya Bean Meal

In the present work, the effect of different carbon sources, namely glucose, sucrose, maltose, fructose and lactose, was studied for extracellular L-asparaginase production by Aspergillus terreus MTCC 1782 in submerged fermentation. The best carbon source and operating conditions such as initial pH, inoculum size, temperature and agitation rate were optimized. Glucose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing L-proline as substrate. Sucrose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing groundnut oil cake flour as substrate. Glucose was found to be the best carbon source for maximum L-asparaginase production using modified Czapek-Dox media containing soya bean meal flour as substrate. The soya bean meal flour was found to be the best natural substrate for maximum L-asparaginase activity of 35.3 IU/mL using 0.6% glucose as carbon source at the optimal culture conditions of initial pH 6, inoculum size 2%, temperature 35°C and agitation rate 160 rpm.

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

scholarly journals Statistical Optimization of Lactic Acid Production by Lactobacillus pentosus using Hemicellulosic Hydrolysate from Sugarcane Bagasse

2018 ◽  
Vol 29 (1) ◽  
pp. 41-51
Author(s):  
Daiana Wischral
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Acid Production ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Hemicellulose Hydrolysate ◽  
Anaerobic Conditions ◽  
Lactobacillus Pentosus

Lactic acid, traditionally obtained through fermentation processes, presents numerous applications in the chemical industry. Among these is the production of polymers, more specifically biodegradable polylactic acid (PLA). Development of processes that use low cost substrates, such as bioproduction of lactic acid, could improve the economic viability of bioprocesses. Thus, the present work reports investigation of hemicellulose hydrolysate from sugarcane bagasse as a sole carbon source for lactic acid production by Lactobacillus pentosus ATCC 8041. Initially, sugarcane bagasse was pretreated with acid in a solid:liquid ratio of 1:2.8 (1 g of bagasse: 2.8 mL of sulfuric acid solution 1 % v/v) and at a temperature of 121°C for 27 minutes. Then, concentration of both the hemicellulose hydrolysate and the yeast extract in MRS medium were optimized usingResponse Surface Methodology through software STATISTICA 6.0. Once the optimal conditions (40 % of hemicellulose hydrolysate and 5 g/L of yeast extract) were validated, fermentations were carried out in anaerobic conditions at 37°C and 120 rpm. After 48h, 19.17 g/L of lactic acid were produced, corresponding to a volumetric productivity of 0.40 g/L.h1. Findings of this work demonstrate that hemicellulose hydrolysate from sugarcane bagasse is a promising carbon source for lactic acid production.

scholarly journals Biodegradation of Basic Yellow Auramine- O Dye using Staphylococcus sp. Isolated from Textile Industry Effluent

2021 ◽  
Vol 14 (4) ◽  
pp. 1571-1576
Author(s):  
Anil R. Shet
Keyword(s):  
Carbon Source ◽  
Textile Industry ◽  
Dye Decolorization ◽  
Inoculum Size ◽  
Synthetic Dyes ◽  
Bacterial Isolates ◽  
Gram Staining ◽  
Initial Ph ◽  
Auramine O ◽  
Industry Effluent

Due to the increased use of synthetic dyes in various industries, there is an increased disposal of wastewater containing harmful dyes. These, in turn, have affected plants, animals, and humans. The physical and chemical methods of dye decolorization have failed to degrade the synthetic dyes in industrial effluents completely. The microbial decolorization is better due to its versatility, dynamic metabolism, and potential machinery of enzymes. This study aimed to degrade basic yellow dye auramine O by bacteria isolated from textile industry effluent. In this regard, five bacterial strains were isolated and screened from a soil sample taken from textile industry effluent. The initial physical and biochemical characterization of the bacterial isolates 1 and 2 indicated catalase test-positive, starch test-negative, motility agar test-negative, gram staining test-positive, and morphology-bacillus. The bacterial isolates 3, 4, and 5 indicated oxidase test-negative, urease test-positive, gram staining test-negative, and morphology-staphylococcus. All the isolates were further subjected to a screening test, where isolate 5 showed maximum dye decolorization of 98.9% in 96 h. The biodegradation of dye was optimized for different values of initial pH (4-10), inoculum size (2% -10%), initial dye concentration (50 mgL-1 to400 mgL-1), carbon source (glucose, fructose, xylose, starch and lactose) and nitrogen source (peptone, ammonium sulphate, yeast extract, ammonium nitrate and urea). Maximum dye decolorization was observed for initial dye concentration of 200 mgL-1, initial pH of 6, inoculum size of 10%, yeast extract as nitrogen source, and glucose as carbon source. Therefore, dye degradation by bacteria can be used as a potential method for auramine O dye treatment.

scholarly journals Novel Methods Using an Arthrobacter sp. to Create Anaerobic Conditions for Biobutanol Production from Sweet Sorghum Juice by Clostridium beijerinckii

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 178
Author(s):  
Chalida Daengbussadee ◽  
Lakkana Laopaiboon ◽  
Anuphon Kaewmaneewat ◽  
Likit Sirisantimethakom ◽  
Pattana Laopaiboon
Keyword(s):  
Sweet Sorghum ◽  
Large Scale ◽  
Clostridium Beijerinckii ◽  
Control Treatment ◽  
Strictly Aerobic ◽  
Strictly Anaerobic ◽  
Aerobic Bacterium ◽  
Butanol Production ◽  
Anaerobic Conditions ◽  
Sweet Sorghum Juice

Biobutanol can be produced by Clostridia via an acetone–butanol–ethanol (ABE) fermentation under strictly anaerobic conditions. Oxygen-free nitrogen (OFN) gas is typically used to create anaerobic conditions for ABE fermentations. However, this method is not appropriate for large-scale fermentations as it is quite costly. The aim of this work was to study the feasibility of butanol production from sweet sorghum juice (SSJ) by Clostridium beijerinckii TISTR 1461 using various methods to create anaerobic conditions, i.e., growth of a strictly aerobic bacterium, an Arthrobacter sp., under different conditions and a chemical method using sodium dithionite (SDTN) to consume residual oxygen. SSJ containing 60 g/L of total sugar supplemented with 1.27 g/L of (NH4)2SO4 was used as a substrate for butanol production. The results showed that 0.25 mM SDTN could create anaerobic conditions, but in this case, C.beijerinckii TISTR 1461 could produce butanol at a concentration (PB) of only 8.51 g/L with a butanol productivity (QB) of 0.10 g/L·h. Arthrobacter sp. BCC 72131 could also be used to create anaerobic conditions. Mixed cultures of C.beijerinckii TISTR 1461 and Arthrobacter sp. BCC 72131 created anaerobic conditions by inoculating the C.beijerinckii 4 h after Arthrobacter. This gave a PB of 10.39 g/L with a QB of 0.20 g/L·h. Comparing butanol production with the control treatment (using OFN gas to create anaerobic conditions, yielding a PB of 9.88 g/L and QB of 0.21 g/L·h) indicated that using Arthrobacter sp. BCC 72131 was an appropriate procedure for creating anaerobic conditions for high levels of butanol production by C. beijerinckii TISTR 1461 from a SSJ medium.

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