scholarly journals Biodegradation of Basic Yellow Auramine- O Dye using Staphylococcus sp. Isolated from Textile Industry Effluent

2021 ◽  
Vol 14 (4) ◽  
pp. 1571-1576
Author(s):  
Anil R. Shet
Keyword(s):  
Carbon Source ◽  
Textile Industry ◽  
Dye Decolorization ◽  
Inoculum Size ◽  
Synthetic Dyes ◽  
Bacterial Isolates ◽  
Gram Staining ◽  
Initial Ph ◽  
Auramine O ◽  
Industry Effluent

Due to the increased use of synthetic dyes in various industries, there is an increased disposal of wastewater containing harmful dyes. These, in turn, have affected plants, animals, and humans. The physical and chemical methods of dye decolorization have failed to degrade the synthetic dyes in industrial effluents completely. The microbial decolorization is better due to its versatility, dynamic metabolism, and potential machinery of enzymes. This study aimed to degrade basic yellow dye auramine O by bacteria isolated from textile industry effluent. In this regard, five bacterial strains were isolated and screened from a soil sample taken from textile industry effluent. The initial physical and biochemical characterization of the bacterial isolates 1 and 2 indicated catalase test-positive, starch test-negative, motility agar test-negative, gram staining test-positive, and morphology-bacillus. The bacterial isolates 3, 4, and 5 indicated oxidase test-negative, urease test-positive, gram staining test-negative, and morphology-staphylococcus. All the isolates were further subjected to a screening test, where isolate 5 showed maximum dye decolorization of 98.9% in 96 h. The biodegradation of dye was optimized for different values of initial pH (4-10), inoculum size (2% -10%), initial dye concentration (50 mgL-1 to400 mgL-1), carbon source (glucose, fructose, xylose, starch and lactose) and nitrogen source (peptone, ammonium sulphate, yeast extract, ammonium nitrate and urea). Maximum dye decolorization was observed for initial dye concentration of 200 mgL-1, initial pH of 6, inoculum size of 10%, yeast extract as nitrogen source, and glucose as carbon source. Therefore, dye degradation by bacteria can be used as a potential method for auramine O dye treatment.

scholarly journals Aspergillus sp. For Indigosol Blue and Remazol Brilliant Blue R Decolorization

2020 ◽  
Vol 2 (3) ◽  
pp. 435
Author(s):  
Fitria Ayudi Ulfimaturahmah ◽  
Ratna Stia Dewi ◽  
Ajeng Arum Sari
Keyword(s):  
Textile Industry ◽  
Environmental Problem ◽  
Ph Value ◽  
Dye Decolorization ◽  
Brilliant Blue ◽  
Blue Dye ◽  
Synthetic Dyes ◽  
Remazol Brilliant Blue R ◽  
Living Organisms ◽  
Aspergillus Sp

Synthetic dyes are artificial dyes manufactured by Industry and commonly used for the textile industry. These dyes had potentially caused an environmental problem. Many types of dyes are recalcitrant and have toxic properties for living organisms. It can be removed by decolorization method, especially a biological decolorization by fungi. Fungi were chosen due to the ability to degrade toxic components. Aspergillus sp. is the fungi which commonly used for dye decolorization. It might be caused that Aspergillus sp. is one type of fungi lived in the textile waste and expected not to die in the dye decolorization treatment. The purpose of this research was to investigate the ability of the mycelia pellets of Aspergillus sp to decolorized Indigosol Blue dye and Remazol Brilliant Blue R (RBBR) dye. This research showed that mycelial pellets of Aspergillus sp. had high activity of decolorization of Indigosol Blue dye up to 85.37% and RBBR dye up to 80.21% and caused low pH value after 24 hour incubation time compared to the control solution.

scholarly journals Bio-degradation of Azo Dye Acid Orange-10 by a New Isolate of Bacillus subtilis Isolated from Soil Sample around Textile Industry in South Karnataka

2021 ◽  
Vol 17 (4) ◽  
pp. 707-716
Author(s):  
Hema Gunti ◽  
Vyshali Venkatappa Maruthiramaih ◽  
Tippeswamy Boreddy Shivanandappa
Keyword(s):  
Bacillus Subtilis ◽  
Textile Industry ◽  
Azo Dye ◽  
Industrial Effluent ◽  
Inoculum Size ◽  
Soil Samples ◽  
Synthetic Dyes ◽  
Acid Orange ◽  
Acid Orange 10 ◽  
Static Conditions

Untreated effluents from the textile industry affect aquatic life irreversibly. Synthetic dyes not only change the color of water resources but also make them hazardous.The main objective of the study was to evaluate the decolorizing potential of a new isolate of Bacillus subtilis from soil samples contaminated with industrial effluent in and around textile industrial area in South Karnataka. This isolate of Bacillus subtilis has high decolorizing potential and took only 24 hrs for complete decolorization of acid orange-10 azo dye at 200ppm. Different parameters like temperature, pH, aeration, dye concentration and inoculum size were optimized for complete decolorization of Acid orange-10 azo dye by this isolate of Bacillus subtilis. The dye was completely decolorized at 400C within 24 hrs and it was capable of decolorizing 700 ppm dye in 72 hrs. Optimum pH was found to be 8.5 and maximum decolorization was achieved under static conditions. As the inoculum size increased, the time taken for complete decolorization of Acid orange-10 dye was decreased from 36 hrs at 1% to 16 hrs at 10% of inoculum size. The new isolate decolorizes 100 ppm of dye completely (i.e.100%) within 12hrs of incubation. The time taken for the complete decolorization increased with increase in the concentration of Acid orange-10 azo dye. In conclusion, the new isolate of Bacillus subtilis from soil samples contaminated with textile industrial effluent was found to be a potential candidate for decolorization of Acid orange-10 azo dye in textile effluents.

scholarly journals Evaluation of biobutanol production by Clostridium beijerinckii NRRL B-592 using sweet sorghum as carbon source

2015 ◽  
Vol 45 (9) ◽  
pp. 1707-1712 ◽  
Author(s):  
Luiz Jardel Visioli ◽  
Eliana Albornoz Alves ◽  
Aline Trindade ◽  
Raquel Cristine Kuhn ◽  
Marcio Schwaab ◽  
...  
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Sweet Sorghum ◽  
Low Cost ◽  
Inoculum Size ◽  
Clostridium Beijerinckii ◽  
Initial Inoculum ◽  
Sweet Sorghum Juice ◽  
Operational Variables ◽  
Initial Ph

<p>In this research it was evaluated the production of biobutanol by<bold> Clostridium beijerinckii</bold>NRRL B-592 using sweet sorghum juice as carbon source. Operational variables, like pH and initial inoculum size, as well as supplementation of industrial media with yeast extract and tryptone, were evaluated. The maximum butanol obtained was 2.12g kg<sup>-1</sup> using 12.5% of inoculum size, 0.05g 100mL<sup>-1</sup> of tryptone and 0.1g 100mL<sup>-1</sup> of yeast extract and initial pH of 5.5. The main contribution of this research was to show a systematic procedure for development of a low cost industrial media for biobutanol production from sweet sorghum.</p>

scholarly journals Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441

2017 ◽  
Vol 75 (7) ◽  
pp. 1572-1583 ◽  
Author(s):  
Ganta Upendar ◽  
Susmita Dutta ◽  
Pinaki Bhattacharya ◽  
Abhishek Dutta
Keyword(s):  
Methylene Blue ◽  
Bacillus Subtilis ◽  
Textile Industry ◽  
Immobilized Cells ◽  
Fixed Bed ◽  
Inoculum Size ◽  
Blue Dye ◽  
Influent Concentration ◽  
Operating Variables ◽  
Industry Effluent

Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20–60 mg/L), inoculum size (4–8%) and temperature (25–35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10–30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.

Production of L-asparaginase from Natural Substrates by Aspergillus terreus MTCC 1782: Optimization of Carbon Source and Operating Conditions

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Baskar Gurunathan ◽  
Renganathan Sahadevan
Keyword(s):  
Carbon Source ◽  
Aspergillus Terreus ◽  
Inoculum Size ◽  
Soya Bean ◽  
Operating Conditions ◽  
Agitation Rate ◽  
Groundnut Oil ◽  
Initial Ph ◽  
Bean Meal ◽  
Soya Bean Meal

In the present work, the effect of different carbon sources, namely glucose, sucrose, maltose, fructose and lactose, was studied for extracellular L-asparaginase production by Aspergillus terreus MTCC 1782 in submerged fermentation. The best carbon source and operating conditions such as initial pH, inoculum size, temperature and agitation rate were optimized. Glucose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing L-proline as substrate. Sucrose was found to be the best carbon source for L-asparaginase production using modified Czapek-Dox media containing groundnut oil cake flour as substrate. Glucose was found to be the best carbon source for maximum L-asparaginase production using modified Czapek-Dox media containing soya bean meal flour as substrate. The soya bean meal flour was found to be the best natural substrate for maximum L-asparaginase activity of 35.3 IU/mL using 0.6% glucose as carbon source at the optimal culture conditions of initial pH 6, inoculum size 2%, temperature 35°C and agitation rate 160 rpm.

scholarly journals Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019 ◽  
Vol 19 (2) ◽  
pp. 470 ◽  
Author(s):  
Siti Nor Amira Rosli ◽  
Rohaida Che Man ◽  
Nasratun Masngut
Keyword(s):  
Carbon Source ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Xylanase Production ◽  
Initial Ph

Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

scholarly journals Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325

2020 ◽  
Author(s):  
Shristi Ram ◽  
Sushma Rani Tirkey ◽  
Madhava Anil Kumar ◽  
Sandhya Mishra
Keyword(s):  
Carbon Source ◽  
Process Parameters ◽  
Regression Coefficient ◽  
Inoculum Size ◽  
Agitation Speed ◽  
Harvest Time ◽  
Bacterial Isolates ◽  
Human Diet ◽  
Ccd Matrix ◽  
Central Composite

Abstract The present study aims to screen potential bacterial isolates for the production of a prophylactic agent zeaxanthin; which is considered to be an inevitable inclusion in human diet. A freshwater bacterium Arthrobacter gandavensis was isolated from Bor Talav, Bhavnagar and screened as a promising producer of zeaxanthin. The chemo-metric tools were employed to optimize the influencing factors such as pH, temperature, inoculum size, agitation speed, carbon source and harvest time on its yield. Thereafter, 6 parameters were narrowed down to 3 factors and were optimized using the central composite design (CCD) matrix. Maximum zeaxanthin (1.51 mg/g) was derived when A. gandavensis was grown under pH 6.0, 1.5% (w/v) glucose and 10% (v/v) inoculum size. A high regression coefficient ( R 2 =0.92) of the developed model indicated the accurateness with the tested parameters. To the best of our knowledge, this is the first report on tailoring of process parameters using chemo-metric optimization for escalating the zeaxanthin production by A. gandavensis.

scholarly journals Decolorization of Synthetic Dyes Using Bacteria Isolated from Textile Industry Effluent

2012 ◽  
Vol 4 (3) ◽  
pp. 129-136 ◽  
Author(s):  
K.R. Mahbub ◽  
A. Mohammad ◽  
M.M. Ahmed ◽  
Salma Begum
Keyword(s):  
Textile Industry ◽  
Synthetic Dyes ◽  
Industry Effluent

scholarly journals Isolation of Fungi from A Textile Industry Effluent and the Screening of Their Potential to Degrade Industrial Dyes

2021 ◽  
Vol 7 (10) ◽  
pp. 805
Author(s):  
Juvenal Juárez-Hernández ◽  
Dalia Castillo-Hernández ◽  
Cristhian Pérez-Parada ◽  
Soley Nava-Galicia ◽  
Jaime Alioscha Cuervo-Parra ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Textile Industry ◽  
Enzyme Production ◽  
Agar Plate ◽  
Ligninolytic Enzymes ◽  
Industrial Effluents ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
Fungal Strains ◽  
Industry Effluent

Six fungal strains were isolated from the textile industry effluent in which they naturally occur. Subsequently, the fungal strains were identified and characterized in order to establish their potential decolorizing effect on textile industry effluents. The strains of interest were selected based on their capacity to decolorize azo, indigo, and anthraquinone dyes. Three of the strains were identified as Emmia latemarginata (MAP03, MAP04, and MAP05) and the other three as Mucor circinelloides (MAP01, MAP02, and MAP06), while the efficiency of their decolorization of the dyes was determined on agar plate and in liquid fermentation. All the strains co-metabolized the dyes of interest, generating different levels of dye decolorization. Plate screening for lignin-degrading enzymes showed that the MAP03, MAP04, and MAP05 strains were positive for laccase and the MAP01, MAP02, and MAP06 strains for tyrosinase, while all strains were positive for peroxidase. Based on its decolorization capacity, the Emmia latemarginata (MAP03) strain was selected for the further characterization of its growth kinetics and ligninolytic enzyme production in submerged fermentation under both enzyme induction conditions, involving the addition of Acetyl yellow G (AYG) dye or wheat straw extract, and no-induction condition. The induction conditions promoted a clear inductive effect in all of the ligninolytic enzymes analyzed. The highest level of induced enzyme production was observed with the AYG dye fermentation, corresponding to versatile peroxidase (VP), manganese peroxidase (MnP), and lignin peroxidase (LiP). The present study can be considered the first analysis of the ligninolytic enzyme system of Emmia latemarginata in submerged fermentation under different conditions. Depending on the results of further research, the fungal strains analyzed in the present research may be candidates for further biotechnological research on the decontamination of industrial effluents.

scholarly journals The Sustainable Production of a Novel Laccase from Wheat Bran by Bordetella sp. JWO16: Toward a Total Environment

Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 677
Author(s):  
John Onolame Unuofin
Keyword(s):  
Wheat Bran ◽  
Contaminated Soils ◽  
Residual Activity ◽  
Dye Decolorization ◽  
Fermentation Medium ◽  
Biochemical Properties ◽  
Synthetic Dyes ◽  
High Concentrations ◽  
Capital Intensive ◽  
Agroindustrial Residue

Laccase is increasingly adopted in diverse industrial and environmental applications, due to its readily accessible requirements for efficient catalytic synthesis and biotransformation of chemicals. However, it is perceived that its industrial production might incur some unfavorable overhead, which leads to expensive market products, and the corresponding negative environmental feedback, due to the use of capital-intensive and precarious chemicals. To this end, this study was designed to evaluate the performance indicators of the valorization of wheat bran by a novel Jb1b laccase and its subsequent application in waste minimization and water management, on a laboratory scale. Optimal Jb1b laccase was produced in submerged fermentation medium containing wheat bran, an agroindustrial residue, through response surface methodology (RSM) algorithm, and was applied in dye decolorization and denim bioscouring, respectively. Results showed that the resultant enzyme manifested unique biochemical properties, such as enhanced tolerance at certain physicochemical conditions, with a residual activity of at least ca. 76%. Furthermore, phenomenally high concentrations of synthetic dyes (0.2% w v−1) were decolorized over 56 h, and a 6 h mediator-supported simultaneous denim bleaching and decolorization of wash effluent was observed. The sustainability of the production and application processes were inferred from the reusability of the fermentation sludge as a potential biofertilizer, with subsequent prospects for the biostimulation and bioaugmentation of contaminated soils, whereas the decolorized water could be adopted for other uses, amongst which horticulture and forestry are typical examples. These phenomena therefore authenticate the favorable environmental feedbacks and overhead realized in this present study.

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