scholarly journals Parameters based on X-ray images to assess the physical and physiological quality of Leucaena leucocephala seeds

2018 ◽  
Vol 42 (6) ◽  
pp. 643-652 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Joyce de Oliveira Araújo ◽  
Manuel Jesús Zavala León ◽  
Laércio Junio da Silva ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Leucaena Leucocephala ◽  
High Performance ◽  
Principal Component ◽  
Automated Analysis ◽  
Radiographic Images ◽  
X Ray ◽  
Physiological Quality ◽  
Non Destructive ◽  
Do So

ABSTRACT Non-destructive and high performance analyses are highly desirable and important for assessing the quality of forest seeds. The aim of this study was to relate parameters obtained from semi-automated analysis of radiographs of Leucaena leucocephala seeds to their physiological potential by means of multivariate analysis. To do so, seeds from five lots collected from parent trees from the region of Viçosa, MG, Brazil, were used. The study was carried out through analysis of radiographic images of seeds, from which the percentage of damaged seeds (predation and fungi), and measurements of area, perimeter, circularity, relative density, and integrated density of the seeds were obtained. After the X-ray test, the seeds were tested for germination in order to assess variables related to seed physiological quality. Multivariate statistics were applied to the data generated, with use of principal component analysis (PCA). X-ray testing allowed visualization of details of the internal structure of seeds and differences regarding density of seed tissues. Semi-automated analysis of radiographic images of Leucaena leucocephala seeds provides information on seed physical characteristics and generates parameters related to seed physiological quality in a simple, fast, and inexpensive manner.

scholarly journals RELATIONSHIP BETWEEN INTERNAL MORPHOLOGY AND PHYSIOLOGICAL QUALITY OF Leucaena leucocephala SEEDS USING IMAGE ANALYSIS

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
André Dantas de Medeiros ◽  
Manuel Jesús Zavala-León ◽  
Joyce de Oliveira Araújo ◽  
Márcio Dias Pereira ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
...  
Keyword(s):  
Leucaena Leucocephala ◽  
Radiographic Images ◽  
X Ray ◽  
Internal Morphology ◽  
Vigor Index ◽  
Measurement Tools ◽  
Physiological Quality ◽  
Non Destructive ◽  
Automated Tool

ABSTRACT The use of new methodological approaches and inclusion of non-destructive evaluations in determining the quality of forest seeds has proven to be of great importance for the development of forest production. The aim of this study was to evaluate the effectiveness of the use of a semi-automated tool for measurement of digital images obtained by the X-ray test in seed lots of Leucaena leucocephala and their relationship to seed physiological quality. Seeds were collected from five mother trees in Viçosa, MG. The study was performed through analysis of radiographic images of the seeds. It was determined the percentage of damaged seeds (predation and fungi) and measured: the area, perimeter, circularity, relative density, and integrated density of the seeds. After the X-ray test, a germination test was performed on the seeds, from which ten variables related to seed physiological quality were extracted, including a vigor index. The use of semi-automated measurement tools associated with the X-ray test was effective for evaluating the internal morphology of Leucaena leucocephala seeds, and the variables determined are related to seed physiological quality.

scholarly journals Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

2021 ◽  
Vol 43 ◽  
Author(s):  
Alessandra da Silva Ribeiro ◽  
Tássia Fernanda Santos Neri ◽  
André Dantas de Medeiros ◽  
Carla do Carmo Milagres ◽  
Laércio Junio da Silva
Keyword(s):  
Seed Quality ◽  
Automated Analysis ◽  
Physical Parameters ◽  
Morphometric Characteristics ◽  
Radiographic Images ◽  
X Ray ◽  
Tissue Integrity ◽  
Seed Embryo ◽  
Physiological Quality

Abstract: Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.

Image Analysis in the Evaluation of the Physical and Physiological Quality of Jiló (Solanum gilo) Seeds during Development

2019 ◽  
pp. 1-10
Author(s):  
Marcus Vinicius Prado Alves ◽  
Édila Vilela De Resende Von Pinho ◽  
Gustavo Costa Prado Alves ◽  
Maria Laene Moreira de Carvalho ◽  
Heloisa Oliveira dos Santos
Keyword(s):  
Image Analysis ◽  
Radiographic Images ◽  
X Ray ◽  
Physiological Quality ◽  
Non Destructive ◽  
Days After Anthesis ◽  
Free Area

Currently, the X-ray image analysis of seeds has proved effective for the evaluation of the physical and physiological quality of the seeds, besides being a non-destructive method. The objective of this work was to evaluate the internal free area of jiló seeds (Solanum gilo) through the analysis of radiographic images and to relate the results with the germination of seeds in different stages of maturation. The seeds were harvested at 35, 40, 45 days after anthesis and 45 days after anthesis with 7 days at rest (45 DAAr). Seeds with free internal area ≤ 10% produced normal seedlings whereas seeds with an average free area above 10% produced abnormal or non-germinated seedlings. Seeds of fruits harvested at 45 DAA and seven days at rest showed a smaller internal free area and a formation of 100% of normal seedlings. The analysis of the radiographic images of jiló seeds allows the measurement of the free internal areas, as well as the determination of the relation between these and the germination.

scholarly journals Physical and physiological quality of Jatropha curcas L. seeds at different maturity stages using image analysis

2020 ◽  
Vol 18 (3) ◽  
pp. e0206
Author(s):  
Daniel T. Pinheiro ◽  
André D. Medeiros ◽  
Manuel J. Zavala-León ◽  
Denise C. F. S. Dias ◽  
Laércio J. Da Silva
Keyword(s):  
Image Analysis ◽  
Jatropha Curcas ◽  
Physical Characteristics ◽  
Radiographic Images ◽  
Seed Filling ◽  
X Ray ◽  
Tissue Integrity ◽  
Maturity Stages ◽  
Physiological Quality

Aim of study: To assess the potential of automated X-ray image analysis to evaluate the physical characteristics of Jatropha curcas seeds, and to relate the parameters obtained with the physiological quality of the seeds harvested at different maturity stages.Area of study: Experimental area of Agronomy Department, Federal University of Viçosa (UFV), Brazil.Material and methods: The fruits were harvested from 20 plants, based on the external skin color (green, yellow, brownish-yellow and brown). The study was performed by automated and visual analysis of radiographic images of the seeds, in which measurements of tissue integrity, density and seed filling were performed. Seed dry matter, germination and seedling growth were also analysed.Main results: Variables obtained through automated analysis of radiographic images correlated significantly with all physiological variables (r > 0.9), as well as visual image evaluations (r > 0.75). The seeds extracted from green fruits presented lower tissue integrity and lower physiological quality. Radiographic analysis was efficient for monitoring J. curcas seed quality at different maturity stages. Morpho-anatomical parameters obtained from X-ray analysis were highly correlated with seed physiological attributes.Research highlights: It is important to develop and improve methodologies based on lower-cost techniques, such as X-ray analysis. In this context, we verified that X-ray images can be used for monitoring J. curcas seed filling and maturation. Radiographic images of seeds can be analyzed automatically with ImageJ software. Internal morphology and physical characteristics of seeds have relationship with their physiological quality.

Monitoring the Maturation of Crambe Seeds Using X-ray Image Analysis

2019 ◽  
pp. 1-7
Author(s):  
Bruno Antonio Lemos De Freitas ◽  
Tássia Fernanda Santos Neri Soares ◽  
André Dantas De Medeiros ◽  
José Geraldo De Araújo Ferreira Filho ◽  
Kamylla Calzolari Ferreira ◽  
...  
Keyword(s):  
Agricultural Sector ◽  
Accelerated Aging ◽  
Automated Analysis ◽  
Morphometric Parameters ◽  
X Ray ◽  
Maturation Stages ◽  
Physiological Quality ◽  
Components Analysis ◽  
Seed Area

The application of modern and efficient techniques to access seed physical and physiological information has contributed to new advances in the agricultural sector. The objective of this study was to evaluate morphometric parameters obtained from the X-Ray analysis using crambe seeds harvested at different maturation stages and to relate them to seed physiological potential. Seeds harvested at different maturation stages were evaluated using X-Ray test, germination, first germination count, germination speed index, root protrusion velocity and accelerated aging. The Principal Components Analysis was applied to data collected. The results demonstrated that the X-Ray analysis allowed to visualize internal morphology of crambe seeds. Differences between seeds harvested at different maturation levels were observed. Correlations between physical variables were verified, such as seed area with germination (r = 0.96). The use of X-Ray analysis combined with semi-automated analysis is efficient to differentiate crambe seeds in different maturation stages. The morphometric parameters are related to the physiological quality of crambe seeds.

scholarly journals X-ray imaging and digital processing application in non-destructive assessing of melon seed quality

2020 ◽  
Vol 42 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Maycon Silva Martins ◽  
Laércio Junio da Silva ◽  
Márcio Dias Pereira ◽  
Manuel Jesús Zavala León ◽  
...  
Keyword(s):  
Large Scale ◽  
Seed Quality ◽  
Automated Analysis ◽  
Digital Processing ◽  
Radiographic Images ◽  
Processing Application ◽  
X Ray ◽  
Digital Radiographs ◽  
Melon Seeds ◽  
Non Destructive

Abstract: Non-destructive and high throughput methods have been developed for seed quality evaluation. The aim of this study was to relate parameters obtained from the free and automated analysis of digital radiographs of hybrid melons’ seeds to their seeds’ physiological potential. Seeds of three hybrid melon (Cucumis melo L.) cultivars from commercial lot samples were used. Radiographic images of the seeds were obtained, from which area, perimeter, circularity, relative density, integrated density and seed filling measurements were generated by means of a macro (PhenoXray) developed for ImageJ® software. After the X-ray test, seed samples were submitted to the germination test, from which variables related to the physiological quality of the seeds were obtained. Variability between lots was observed for both physical and physiological characteristics. Results showed that the use of the PhenoXray macro allows large-scale phenotyping of seed radiographs in a simple, fast, consistent and completely free way. The methodology is efficient in obtaining morphometric and tissue integrity data of melon seeds and the generated parameters are closely related to physiological attributes of seed quality.

scholarly journals ASSESSMENT OF THE PHYSICAL AND PHYSIOLOGICAL QUALITY OF Piptadenia gonoacantha SEEDS (MART.) J. F. MACBR. USING IMAGE ANALYSIS

2020 ◽  
Vol 44 ◽  
Author(s):  
Daniel Teixeira Pinheiro ◽  
Nayara Pereira Capobiango ◽  
André Dantas de Medeiros ◽  
Karine Fernandes Caiafa ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
...  
Keyword(s):  
Image Analysis ◽  
Visual Analysis ◽  
New Technologies ◽  
Automated Analysis ◽  
Quality Analysis ◽  
Native Forest ◽  
X Ray ◽  
Tissue Density ◽  
Physiological Quality

ABSTRACT The assessment of the quality of native forest species seeds is extremely important and, in this sense, it is necessary to integrate new technologies that can complement the quality analysis traditionally used. This study aimed to evaluate the physical and physiological quality of Piptadenia gonoacantha seeds using image analysis techniques and to relate aspects of tissue integrity with physiological attributes. Seeds from 10 lots were collected and submitted to automatic and visual analyses of radiographic images, with the determination of parameters related to tissue density and seed filling. After the X-ray test, the seeds were subjected to physiological analyses of germination and vigor. It was observed that the lots that presented higher values of tissue density, filling, and solidity, presented higher germination and vigor. It was also evident that the damage present in the seeds was causally related their physiological condition. Therefore, the results showed that the use of the X-ray technique in seeds combined with the automated analysis of the radiographs is efficient in the evaluation of the internal morphology of P. gonoacantha seeds. The physical integrity of the seed tissues is directly related to the physiological quality and allows the classification of different lots as to vigor. Visual analysis of digital radiographs is adequate to identify malformation in P. gonoacantha seeds.

scholarly journals Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Min Wei ◽  
Yangling Tuo ◽  
Ye Zhang ◽  
Qi Deng ◽  
Cuiying Shi ◽  
...  
Keyword(s):  
Evaluation System ◽  
High Performance ◽  
Comprehensive Evaluation ◽  
Principal Component ◽  
Dry Weight ◽  
Quantification Analysis ◽  
Development And Utilization ◽  
Array Detection ◽  
Accurate Quantification

The purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, filter out the key compounds, and evaluate the quality of 26 batches of tender leaves and flower spikes of Lithocarpus polystachyus Rehd. (LP) from ten areas in China. The result showed that the HPLC-DAD method had excellent performance for accurate quantification analysis. S3 (tender leaf from Lushan, Sichuan) had the highest contents for six measured chemicals with trilobatin content of up to 27.82% in dry weight. S22 (flower spike from Liangping, Chongqing) had the highest content of phloridzin (up to 7.28%). All samples were divided into three types based on spatial distribution using principal component analysis. The result showed that the tender leaves and flower spikes from the same areas had many similar properties, and there were significant differences between the samples from different regions. Furthermore, phloridzin and trilobatin were identified as chemical markers for quality evaluation of two parts with different tender leaves and flower spikes of LP from geographical areas by orthogonal partial least squares discrimination analysis. These results will be helpful to establish an effective and comprehensive evaluation system of the development and utilization of LP resources.

Application of Visible-Near Infrared Spectra Technique Based on to the Non-Destructive Measurement of the Salmon Quality

2014 ◽  
Vol 926-930 ◽  
pp. 961-964
Author(s):  
Jiao Jiao Yin
Keyword(s):  
Infrared Spectra ◽  
Near Infrared ◽  
Principal Component ◽  
Support Vector ◽  
Analysis Method ◽  
Linear Discriminant ◽  
Near Infrared Spectra ◽  
Destructive Measurement ◽  
Non Destructive

Because the reflectivity of astaxanthin vary in different bands (mainly 400nm-600nm), so we use the visible-near infrared spectra technique to irradiate the salmon. Because in daily life, people grade the salmon flesh with a color card. In this paper, we first use principal component analysis to reduce the dimensionality of the spectral data of salmon, then use linear discriminant analysis method, least squares support vector machine classification method to distinguish the flesh quality. The correct classification rates are 60%and73.3%. The results show that we can use visible – near infrared spectra to distinguish the quality of the salmon which doesn’t be dissected.

scholarly journals Seed quality analysis of Senna siamea Lam. using image analysis techniques

2020 ◽  
Vol 42 ◽  
Author(s):  
Jackson Araújo da Silva ◽  
André Dantas de Medeiros ◽  
Márcio Dias Pereira ◽  
Amanda Karoliny Fernandes Ramos ◽  
Laércio Junio da Silva
Keyword(s):  
Image Analysis ◽  
Seed Quality ◽  
Quality Analysis ◽  
Radiographic Images ◽  
Senna Siamea ◽  
Analysis Techniques ◽  
Physiological Quality ◽  
Image Analysis Techniques ◽  
Forest Seeds

Abstract: The inclusion of image analysis techniques for the accurate and rapid assessment of the quality of forest seeds is highly desirable. The use of digital radiographic images of seeds and the analysis of seedling images, still little used in determining the quality of forest seeds, are among the available imaging techniques. This study aimed to assess the feasibility of using the analysis of images of seeds and seedlings in the assessment of the physical and physiological quality of Senna siamea seeds. Radiographic images of seeds were obtained from five seed lots, allowing generating morphological and tissue integrity descriptors. These seeds were then subjected to germination and seedling growth tests, which allowed obtaining variables related to physiological quality. The generated seedlings were scanned and analyzed using the software ImageJ. The data were analyzed using analysis of variance, correlation, and principal component analysis. The results showed differences between seed lots in terms of physiological quality and physical integrity of internal tissues. Significant correlations were observed between the variables obtained with the radiographic analysis and seed physiological characterization tests. The use of techniques to analyze seed radiographs and seedling images allows access to information on the physical and physiological integrity of S. siamea seeds.

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