Monitoring the Maturation of Crambe Seeds Using X-ray Image Analysis

2019 ◽  
pp. 1-7
Author(s):  
Bruno Antonio Lemos De Freitas ◽  
Tássia Fernanda Santos Neri Soares ◽  
André Dantas De Medeiros ◽  
José Geraldo De Araújo Ferreira Filho ◽  
Kamylla Calzolari Ferreira ◽  
...  
Keyword(s):  
Agricultural Sector ◽  
Accelerated Aging ◽  
Automated Analysis ◽  
Morphometric Parameters ◽  
X Ray ◽  
Maturation Stages ◽  
Physiological Quality ◽  
Components Analysis ◽  
Seed Area

The application of modern and efficient techniques to access seed physical and physiological information has contributed to new advances in the agricultural sector. The objective of this study was to evaluate morphometric parameters obtained from the X-Ray analysis using crambe seeds harvested at different maturation stages and to relate them to seed physiological potential. Seeds harvested at different maturation stages were evaluated using X-Ray test, germination, first germination count, germination speed index, root protrusion velocity and accelerated aging. The Principal Components Analysis was applied to data collected. The results demonstrated that the X-Ray analysis allowed to visualize internal morphology of crambe seeds. Differences between seeds harvested at different maturation levels were observed. Correlations between physical variables were verified, such as seed area with germination (r = 0.96). The use of X-Ray analysis combined with semi-automated analysis is efficient to differentiate crambe seeds in different maturation stages. The morphometric parameters are related to the physiological quality of crambe seeds.

scholarly journals Parameters based on X-ray images to assess the physical and physiological quality of Leucaena leucocephala seeds

2018 ◽  
Vol 42 (6) ◽  
pp. 643-652 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Joyce de Oliveira Araújo ◽  
Manuel Jesús Zavala León ◽  
Laércio Junio da Silva ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Leucaena Leucocephala ◽  
High Performance ◽  
Principal Component ◽  
Automated Analysis ◽  
Radiographic Images ◽  
X Ray ◽  
Physiological Quality ◽  
Non Destructive ◽  
Do So

ABSTRACT Non-destructive and high performance analyses are highly desirable and important for assessing the quality of forest seeds. The aim of this study was to relate parameters obtained from semi-automated analysis of radiographs of Leucaena leucocephala seeds to their physiological potential by means of multivariate analysis. To do so, seeds from five lots collected from parent trees from the region of Viçosa, MG, Brazil, were used. The study was carried out through analysis of radiographic images of seeds, from which the percentage of damaged seeds (predation and fungi), and measurements of area, perimeter, circularity, relative density, and integrated density of the seeds were obtained. After the X-ray test, the seeds were tested for germination in order to assess variables related to seed physiological quality. Multivariate statistics were applied to the data generated, with use of principal component analysis (PCA). X-ray testing allowed visualization of details of the internal structure of seeds and differences regarding density of seed tissues. Semi-automated analysis of radiographic images of Leucaena leucocephala seeds provides information on seed physical characteristics and generates parameters related to seed physiological quality in a simple, fast, and inexpensive manner.

scholarly journals Assessing the physiological quality of common bean seeds using the Vigor-S® system and its relation to the accelerated aging test

2019 ◽  
Vol 41 (2) ◽  
pp. 187-195 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Laércio Junio da Silva ◽  
Nayara Pereira Capobiango ◽  
Camila Andrade Fialho ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Common Bean ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Growth Index ◽  
Automated Analysis ◽  
Seed Vigor ◽  
Analysis Technique ◽  
Physiological Quality

Abstract: Automated analysis of seed vigor stands out by allowing greater accuracy, standardization, objectivity, and speed in evaluation of the physiological potential of seed lots. The objective of this study was to evaluate the efficiency of the Vigor-S® system in assessing the physiological quality of common bean seeds compared to the information provided by the traditional vigor tests recommended for this species. Four genotypes of common bean were used, each one represented by four seed lots. Characterization of the physiological potential of the lots was carried out by the following tests: germination, first count of germination, seedling emergence, accelerated aging, and electrical conductivity. The results of these tests were compared with the data obtained from the image analysis technique, specifically the Vigor-S® system, which was used to evaluate seedling growth at two, three, and four days after the beginning of the germination test. Shoot length, primary root length, and seedling length were measured, as well as the growth index, uniformity index, and vigor index were calculated. Computerized analysis of seedling images using the Vigor-S® software is a reliable alternative for evaluation the physiological potential of bean seeds, and it produces information similar to evaluations traditionally used for that purpose.

scholarly journals Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

2021 ◽  
Vol 43 ◽  
Author(s):  
Alessandra da Silva Ribeiro ◽  
Tássia Fernanda Santos Neri ◽  
André Dantas de Medeiros ◽  
Carla do Carmo Milagres ◽  
Laércio Junio da Silva
Keyword(s):  
Seed Quality ◽  
Automated Analysis ◽  
Physical Parameters ◽  
Morphometric Characteristics ◽  
Radiographic Images ◽  
X Ray ◽  
Tissue Integrity ◽  
Seed Embryo ◽  
Physiological Quality

Abstract: Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.

scholarly journals ASSESSMENT OF THE PHYSICAL AND PHYSIOLOGICAL QUALITY OF Piptadenia gonoacantha SEEDS (MART.) J. F. MACBR. USING IMAGE ANALYSIS

2020 ◽  
Vol 44 ◽  
Author(s):  
Daniel Teixeira Pinheiro ◽  
Nayara Pereira Capobiango ◽  
André Dantas de Medeiros ◽  
Karine Fernandes Caiafa ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
...  
Keyword(s):  
Image Analysis ◽  
Visual Analysis ◽  
New Technologies ◽  
Automated Analysis ◽  
Quality Analysis ◽  
Native Forest ◽  
X Ray ◽  
Tissue Density ◽  
Physiological Quality

ABSTRACT The assessment of the quality of native forest species seeds is extremely important and, in this sense, it is necessary to integrate new technologies that can complement the quality analysis traditionally used. This study aimed to evaluate the physical and physiological quality of Piptadenia gonoacantha seeds using image analysis techniques and to relate aspects of tissue integrity with physiological attributes. Seeds from 10 lots were collected and submitted to automatic and visual analyses of radiographic images, with the determination of parameters related to tissue density and seed filling. After the X-ray test, the seeds were subjected to physiological analyses of germination and vigor. It was observed that the lots that presented higher values of tissue density, filling, and solidity, presented higher germination and vigor. It was also evident that the damage present in the seeds was causally related their physiological condition. Therefore, the results showed that the use of the X-ray technique in seeds combined with the automated analysis of the radiographs is efficient in the evaluation of the internal morphology of P. gonoacantha seeds. The physical integrity of the seed tissues is directly related to the physiological quality and allows the classification of different lots as to vigor. Visual analysis of digital radiographs is adequate to identify malformation in P. gonoacantha seeds.

scholarly journals Genetic diversity for physiological quality of seeds from corn (Zea mays L.) intervarietal crossbreeds

2013 ◽  
Vol 35 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Rubens Onofre Nodari
Keyword(s):  
Genetic Diversity ◽  
Zea Mays L ◽  
Accelerated Aging ◽  
Genetic Contribution ◽  
Percentage Germination ◽  
Canonical Variables ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.

scholarly journals Compatibility of Azospirillum brasilense with fungicide and insecticide and its effects on the physiological quality of wheat seeds

2018 ◽  
Vol 39 (2) ◽  
pp. 855 ◽  
Author(s):  
Janete Denardi Munareto ◽  
Thomas Newton Martin ◽  
Tania Maria Müller ◽  
Ubirajara Russi Nunes ◽  
Guilherme Bergeijer da Rosa ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Seed Treatment ◽  
Accelerated Aging ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Pests And Diseases ◽  
Physiological Quality ◽  
Growth Promoting ◽  
Germination Vigor

Seed treatment is a practice that helps the initial establishment of the crop without the effects caused by pests and diseases. The association of diazotrophic bacteria with grasses has been used in the supply of nitrogen to plants; however, these microorganisms produce growth-promoting substances, which promote benefits in the growth and development of the crops. Thus, the objective of this study was to evaluate the compatibility of Azospirillum brasilense associated with the fungicide difenoconazole and the insecticide thiamethoxam by observing the effects on the quality of seed emergence of three wheat cultivars (Triticum aestivum L.). Three wheat cultivars, arranged in a 4 x 2 factorial system with four replicates, were tested. The treatments were the control; difenoconazole (Spectro® at a dose of 150 mL per 100 kg of seed); thiamethoxam (Cruiser® FS 350 at a dose of 200 mL per 100 kg seed) and difenoconazole + thiamethoxam, applied on wheat cultivars TBIO Mestre, TBIO Itaipu and TBIO Sinuelo. Bacteria from the Azospirillum genus were used in the inoculation. The wheat seed retains its quality when it checks the germination, vigor and independent accelerated aging, whether or not fungicide, insecticide and A. brasilense were used. The insecticide thiamethoxam increased the length of shoots and roots and provided compatibility with A. brasilense, and fungicide inhibited the length of shoots and roots and was antagonistic to the bacterium A. brasilense.

scholarly journals Lignification of the plant and seed quality of RR soybeans sprayed with herbicide glyphosate

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 286-292
Author(s):  
Cristiane Fortes Gris ◽  
Edila Vilela de Resende Von Pinho ◽  
Maria Laene de Moreira Carvalho ◽  
Rafael Parreira Diniz ◽  
Thaís de Andrade
Keyword(s):  
Seed Quality ◽  
Lignin Content ◽  
Block Design ◽  
Accelerated Aging ◽  
Soybean Seeds ◽  
Randomized Block Design ◽  
Physiological Quality ◽  
Hand Weeding ◽  
Herbicide Glyphosate

Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, however, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds. The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate. The assays were conducted both in greenhouse and field in the municipality of Lavras, MG, in the agricultural year 2007/08. The experiment was arranged in a splitplot design with four replicates, considering the treatments hand weeding and herbicide glyphosate as plots, and five RR soybean cultivars (BRS 245 RR, BRS 247 RR, Valiosa RR, Silvânia RR and Baliza RR) as splitplots. In the greenhouse, the cultivars tested were BRS 245 RR and Valiosa RR in a randomized block design with four replicates. The sprayings were carried out at stages V3, V7 and early R5 (3L/ha). The 1000 seed weight, mechanical injury, germination and germination velocity index, emergence velocity index, accelerated aging, electrical conductivity and water soaking seed test, lignin content in the seed coat, in the stem and legumes were determined. The spraying of glyphosate herbicide, in greenhouse and field, did not alter the physiological quality of seeds and the lignin contents in the plant.

scholarly journals Foliar application of silicon and the effect on wheat seed yield and quality

2013 ◽  
Vol 35 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Samantha Rigo Segalin ◽  
Caroline Huth ◽  
Thais D'Avila Rosa ◽  
Diógenes Barella Pahins ◽  
Liliane Marcia Mertz ◽  
...  
Keyword(s):  
Seed Yield ◽  
Foliar Application ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Wheat Cultivars ◽  
Wheat Seed ◽  
Germination Test ◽  
Yield And Quality ◽  
Physiological Quality

Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars.

scholarly journals PRODUCTION AND PHYSIOLOGICAL QUALITY OF SOYBEAN SEEDS IN ORTHIC QUARTZARENIC NEOSOIL OF THE CERRADO REGION

2015 ◽  
Vol 28 (4) ◽  
pp. 127-134
Author(s):  
CHARLINE ZARATIN ALVES ◽  
GUSTAVO MARTINS ZAQUEU ◽  
ERIC FABIANO SERAGUZI ◽  
AGUINALDO JOSÉ FREITAS LEAL ◽  
JOSUÉ BISPO DA SILVA
Keyword(s):  
Electrical Conductivity ◽  
Aerial Part ◽  
Water Distribution ◽  
Accelerated Aging ◽  
Dry Mass ◽  
Soybean Seeds ◽  
Scheme Design ◽  
Physiological Quality ◽  
Tetrazolium Test

ABSTRACT: The aim of this study was to evaluate the effect of the sowing periods in production, productivity component and physiological quality of seeds of three soybean cultivars (TMG133RR, P98Y70RR and NS7670RR) in Orthic Quartzarenic Neosoil in the cerrado region. The design used was a randomized block a factorial scheme design with four repetitions, and each plot with useful space consisted of three rows of four meters length, spaced at 0.45 meters. Field evaluations were the final stand, the height of the plants, height of the first pod insertion, the mass of 100 seeds and productivity. Already in the lab seeds were evaluated for germination and vigor (first germination count, emergency, emergence speed index, length and dry mass of the aerial part of the plant and roots, electrical conductivity, accelerated aging and tetrazolium test). It concludes that it is possible to use Orthic Quartzarenic Neosoils, located in the Cerrado region at altitudes higher than 600 m, in years and places with good water distribution in the spring-summer seasons, for grain and soybeans production, but as a first goal, the crop should be sown in the first 20 days of November otherwise it should take place in early December.

scholarly journals Effect of seed vigor and sowing densities on the yield and physiological potential of wheat seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli
Keyword(s):  
Seed Yield ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Crop ◽  
Seedling Length ◽  
Physiological Quality ◽  
Initial Seed ◽  
Wheat Seeds

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.

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