scholarly journals Evaluation of the effects of sugarcane molasses as a carbon source for Ankistrodesmus gracilis and Haematococcus pluvialis (Chlorophyceae)

2020 ◽  
Vol 80 (3) ◽  
pp. 594-600
Author(s):  
L. H. Sipaúba-Tavares ◽  
M. G. Tedesque ◽  
B. Scardoeli-Truzzi
Keyword(s):  
Carbon Source ◽  
Cell Density ◽  
Culture Medium ◽  
High Cell Density ◽  
Culture Media ◽  
Haematococcus Pluvialis ◽  
Sugarcane Molasses ◽  
Protein Levels ◽  
Microalgae Culture ◽  
Alternative Carbon Source

Abstract Two species of Chlorophyceae, Ankistrodesmus gracilis and Haemotococcus pluvialis, were used to compare and evaluate the effect of sugarcane molasses as a carbon source. Highest cell density in the two microalgae culture media was obtained in commercial culture media (CHU12 and WC). During exponential growth (day 1 to day 10), high cell density in H. pluvialis was detected for E. crassipes culture medium ranging between 0.4 x 105 cells mL-1 and 1.7 x 105 cells mL-1. Culture media were fundamental for growth under mixotrophic cultivation. Sugarcane molasses showed different results for the two microalgae with regard to growth performance, lipid and protein levels. Rates were high for H. pluvialis except lipid at the end of the experiment. In fact, A. gracilis presented almost double the lipid levels. Sugarcane molasses may be an alternative carbon source in laboratory conditions.

A role for rhamnolipid in biofilm dispersion

Biofilms ◽  
2004 ◽  
Vol 1 (2) ◽  
pp. 91-99 ◽  
Author(s):  
S. R. Schooling ◽  
U. K. Charaf ◽  
D. G. Allison ◽  
P. Gilbert
Keyword(s):  
Surface Tension ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Culture Medium ◽  
High Cell Density ◽  
Culture Media ◽  
Homoserine Lactone ◽  
Wild Type ◽  
In The Wild ◽  
Biofilm Dispersion

Biofilms are often considered as localized zones of high cell density. Quorum sensing provides a means for control of population processes and has been implicated in the regulation of biofilm activities. We present a role for quorum sensing in programmed detachment and dispersal processes. Biofilms of Pseudomonas aeruginosa PAO1 and its isogenic homoserine lactone (HSL) mutant P. aeruginosa PAO-JP2 were grown in batch culture on glass substrata; differences were found in the rate and extent of formation of biofilm. Climax communities were observed for PAO1 at 24 h. These were later accompanied by foaming, a drop in the surface tension of culture media and dispersal of the biofilm, after which no subsequent biofilm accretion occurred. PAO-JP2 cultures reformed biofilm post-detachment and did not foam. Prevention of biofilm reformation in the wild type was related to some component excreted into the culture medium. Rhamnolipid, a biosurfactant regulated by quorum sensing, was detected in PAO1 cultures. When rhamnolipid was added to freshly inoculated substrata, biofilm formation was inhibited. At 20 h, PAO1 biofilms were transferred to medium with added rhamnolipid: biofilm was relatively unaffected. Biofilm events were also studied in medium supplemented with N-butyryl-L-homoserine lactone, which is involved in the regulation of rhamnolipid synthesis. Both strains exhibited similar trends of rapid biofilm formation and dramatic changes in the rate and extent of biofilm accretion. In both cases, there was premature foaming, lowered surface tension and elevated rhamnolipid levels. A role for HSLs in maintenance of biofilm and events leading to dispersion of cells is proposed. This role would encompass dispersion but not necessarily detachment of cells from biofilm and supports a new function for rhamnolipid in pathogenesis, whereby rhamnolipid would promote the dissemination of cells from a nidus of infection.

Water Relations and Composition of Wheat Ears Grown in Liquid Culture: Effect of Carbon and Nitrogen

1983 ◽  
Vol 10 (1) ◽  
pp. 99 ◽  
Author(s):  
EWR Barlow ◽  
GR Donovan ◽  
JW Lee
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Sucrose Concentration ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Protein Nitrogen ◽  
Protein Levels ◽  
Grain Starch ◽  
Osmotic Potentials

Detached wheat ears were grown, under lighted controlled conditions, in liquid culture media containing different concentrations of sucrose and amino acid. Higher levels of sucrose in the culture medium resulted in decreased transpiration, with large accumulations of soluble sugar in the bracts. The sugar accumulations led to decreased total and osmotic potentials of the bracts, but turgor was maintained or increased. The concentration of sucrose or nitrogen in the culture medium had no significant effect on the water or osmotic potentials of the grain. Soluble sugar concentrations in the grain were not affected by increasing the sucrose concentration in the culture medium. Increasing the nitrogen level in the culture medium led to increased concentrations of total nitrogen and non- protein nitrogen in the grain. Higher protein levels in the grain resulted in increased water content but no significant changes in the water potential of the grain. The grain was the strongest nitrogen sink within the ear and, within the grain, starch and protein synthesis were independent of each other. Proline accumulated in the bracts and to a lesser extent in the rachis in response to the accumulation of soluble sugars and nitrogen rather than to the low osmotic or total water potentials. Little proline accumulated in the grain and this was presumed to be due to translocation from other parts of the ear rather than to in situ synthesis.

scholarly journals Kandungan Protein Spirulina platensis Pada Media Kultur Dengan Konsentrasi Nitrat (KNO3 ) Yang Berbeda

2018 ◽  
Vol 7 (2) ◽  
pp. 98
Author(s):  
Saniyatul Ulya ◽  
Sri Sedjati ◽  
Ervia Yudiati
Keyword(s):  
Protein Content ◽  
Spirulina Platensis ◽  
Culture Medium ◽  
Culture Media ◽  
Nitrate Concentration ◽  
Block Design ◽  
Quality Measurement ◽  
Daily Basis ◽  
Natural Food ◽  
Protein Levels

 Spirulina platensis merupakan mikroalga hijau biru yang mengandung nutrisi protein tinggi sehingga banyak digunakan sebagai pakan alami. Pertumbuhan dan kandungan protein mikroalga dipengaruhi oleh beberapa faktor, salah satunya adalah pemberian makronutrien pada media kultur mikroalga. Penelitian ini bertujuan untuk melihat pengaruh pertumbuhan dan kandungan protein pada mikroalga S. platensis dengan pemberian konsentrasi nitrat yang berbeda. Rancangan penelitian yang digunakan adalah rancangan acak blok dengan tiga kali pengulangan. Perlakuan konsentrasi nitrat berbeda yang diberikan adalah 50 ppm, 100 ppm, dan 150 ppm. Perhitungan kepadatan dan pengukuran parameter kualitas air dilakukan setiap hari. Pemanenan dilakukan pada hari ke – empat. Kadar prorein dianalisis dengan menggunakan metode Kjedahl. Hasil penelitian pertumbuhan S. platensis menunjukkan nilai kepadatan sel S. platensis tertinggi pada hari ke – empat berada pada perlakuan C dengan konsentrasi nitrat 150 ppm (169,58 . 103 sel/mm3). Hasil analisis ANOVA menunjukkan bahwa konsentrasi nitrat berpengaruh terhadap pertumbuhan S. platensis (p < 0,05) namun perbedaan konsentrasi nitrat tidak berpengaruh pada kadar protein (p ≥ 0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa konsentrasi nitrat yang ditambahkan pada media kultur S. platensis berpengaruh terhadap pertumbuhan S. platensis namun tidak berpengaruh pada kandungan proteinnya.  Protein Content of Spirulina platensis in Different Culture Media with Nitrate (KNO3) Concentration Spirulina platensis is green-blue microalgae that contain high protein nutrient and could be used as natural food. Growth and protein content of microalgae are influenced by several factors and one of those is giving macronutrient to microalgae’s culture medium. The purpose of this research is to compare the growth and protein content of the S. platensis with different nitrate consentrations.The research design used was a completely randomized block design with three repetitions. The different nitrate concentration treatments were 50 ppm, 100 ppm and 150 ppm. Determination of density and water quality measurement parameters was done on daily basis. Spirulina platensis was harvested done on fourth day of culture. Protein levels were analyzed by Kjedahl method. The result of the S. platensis growth that the highest density on day fourth in C treatment with 150 ppm nitrate consentration (169,58 . 103 sel/mm3). The result of ANOVA analysis show that the concentration of nitrate affected on S. platensis growth (p < 0,05) but the difference of nitrate concentration wasn’t affected in protein analysis (p ≥ 0,05). Based on the result of this research, it can be concluded that the concentration of nitrate added to the S. platensis culture medium effectively and improved the growth of S. platensis but had no effect on the protein content. 

scholarly journals Aquaculture biological waste as culture medium to cultivation of Ankistrodesmus gracilis (Reinsch) Korshikov

2017 ◽  
Vol 78 (3) ◽  
pp. 579-587 ◽  
Author(s):  
L. H. Sipaúba-Tavares ◽  
T. Florêncio ◽  
B. Scardoeli-Truzzi
Keyword(s):  
Chlorophyll A ◽  
Cell Density ◽  
Total Phosphorus ◽  
Biochemical Composition ◽  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Conventional Culture ◽  
Significant Difference ◽  
Biological Wastes

Abstract Current study investigated the effectiveness of different macrophytes as culture media for Ankistrodesmus gracilis in laboratory conditions. Significant difference (p < 0.05) was reported in cell density with regard to conventional culture medium (CHU12) and macrophytes culture media. Mean cell density in NPK, Eichhornia crassipes and E. azurea media was higher (p < 0.05) than in conventional culture medium. Chlorophyll-a was higher than 1 g.L-1, except in CHU12 (0.7 ± 0.4 g.L-1) and T. domingensis (0.8 ± 0.3 g.L-1) media. Nitrate decreased sharply as from the 7th-day of the experiment. Ammonium and total phosphorus were highest in culture media and ranged between 0.4 g.L-1 (P. cordata medium) and 1.7 g.L-1 (CHU12 medium) for ammonium, and between 0.8 g.L-1 (CHU12 medium) and 1.9 g.L-1 (T. domingensis medium) for total phosphorus. Results revealed inorganic fertilizer and macrophytes combined with vitamins may be effective as culture media and strongly supports the growth of Ankistrodesmus gracilis, since cell density and biochemical composition are similar to or higher than conventional culture medium (CHU12). Macrophyte is a tool for aquaculture since biological wastes may be used with nutrients to improve the cultivation of microalgae.

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