Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

Abstract The essential oil (EO) composition of leaves of Campomanesia aurea was analyzed by GC-MS. The plant was collected in April (EOA) and October (EOO) in São Francisco de Assis/RS. The essential oils were obtained by hydrodistillation in a modified Clevenger apparatus. The yields were 4.44% in April, and 6.15%, in October. Thirty-one compounds were identified in EOA, accounting for 96.87% of the total, and twenty-eight compounds were identified in EOO, accounting for 99.46% of the total. For EOA, the major identified monoterpene was p-cymene (8.33%) and the major identified sesquiterpene was α-cadinol (10.72%), while regarding EOO the major identified monoterpene was repeatedly Terpinolene (3.43%), whereas the major identified sesquiterpene was α-cadinol (12.79%). The antibacterial activity of the essential oil was evaluated against the three Listeria monocytogenes from the American Type Culture Collection (strains ATCC 7644, 19114 and 13932). The essential oil showed a MIC of 10 mg/mL against the strains ATCC 7644 and 19114, with bacteriostatic effect of 20 mg/mL and a MIC of 20 mg/mL for ATCC 13932. Regarding the antibiofilm activity, o EOO showed the best results with an inhibition of 90% in the sessile growth with an applied concentration of 4.0 mg/mL.

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Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x17751314 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1775131 ◽

Cited By ~ 7

Author(s):

Farhad Sharafati Chaleshtori ◽

Mohamad Saholi ◽

Reza Sharafati Chaleshtori

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Antioxidant Power ◽

Listeria Species ◽

Bunium Persicum

This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp. L monocytogenes was found to be resistant to ampicillin, amoxicillin/clavulanic acid, penicillin, vancomycin, and kanamycin. B persicum essential oil showed the greatest antioxidant activity (248.56 ± 1.09 µM Fe2+/g). The E globulus essential oil showed consistently strong antimicrobial activity against L monocytogenes and L grayi, while rose water showed no antimicrobial activity against any of the tested bacterial strains. The results showed that after adding the B persicum and E globulus essential oils to bacteria, the cell components’ release increased significantly.

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IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s5.t0084 ◽

2019 ◽

pp. 113-115

Author(s):

PURIT PATTANAPANIT ◽

SUNISA MITHONGLANG ◽

SUNITA MITHONGLANG ◽

SURACHAI TECHAOEI

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Aromatic Plants ◽

Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

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Chemical Composition and Antibacterial Activity of the Essential Oils of Blainvillea Rhomboidea (Asteraceae)

Natural Product Communications ◽

10.1177/1934578x0600100510 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100

Author(s):

Andreza Maria L. Pires ◽

Maria Rose Jane R. Albuquerque ◽

Edson P. Nunes ◽

Vânia M. M. Melo ◽

Edilberto R. Silveira ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Caryophyllene Oxide ◽

Bacterial Strains ◽

Aerial Parts ◽

Leaf Oil

The essential oils of Blainvellea rhomboidea (Asteraceae) were obtained by hydrodistillation and analyzed by GC/MS and GC/FID. Initially, the essential oil from the aerial parts was investigated. From the 18 identified components, 5-indanol (14.5%) followed by p-cymen-8-ol (10.1%), β-caryophyllene (9.6%), caryophyllene oxide (9.6%), limonene (8.6%), terpinolene (7.8%), and spathulenol (7.7%) were the major constituents. The oil was tested against seven bacterial strains and the results showed significant antimicrobial activity. As a consequence, the essential oils from leaves and from flowers were analyzed separately. The major constituents of the leaf oil were terpinolene (21.2%), β-caryophyllene (19.2%), spathulenol (9.1%), caryophyllene oxide (7.4%), and bicyclogermacrene (7.1%), while the oil of the flowers contained terpinolene (28.1%), 5-indanol (16.3%), p-cymen-8-ol (15.3%) and limonene (14.7%) as prevalent compounds. The oils were tested against the same bacterial strains and the flower oil was the more active. These results indicated that the components of the essential oil from flowers seem to be responsible for the activity.

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Garlic, Onion, and Cinnamon Essential Oil Anti-Biofilms’ Effect against Listeria monocytogenes

Foods ◽

10.3390/foods9050567 ◽

2020 ◽

Vol 9 (5) ◽

pp. 567 ◽

Cited By ~ 3

Author(s):

Mariem Somrani ◽

María-Carmen Inglés ◽

Hajer Debbabi ◽

Ferid Abidi ◽

Alfredo Palop

Keyword(s):

Listeria Monocytogenes ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Concentration ◽

Cell Attachment ◽

Antibiofilm Activity ◽

Initial Cell ◽

Food Industries ◽

Crystal Violet Assay ◽

Cinnamon Essential Oil

Biofilms represent a serious problem for food industries due to their persistence in processing surfaces, from which they can cause food spoilage or, even worse, lead to foodborne diseases. Microorganisms immersed in biofilms are more resistant to biocides. The search for natural effective alternatives for the prevention and the control of biofilms has increased lately. The aim of this research was to test the antibacterial and the anti-biofilm activities of cinnamon, onion, and garlic essential oils against Listeria monocytogenes. The methodology highlighted first the effect of these essential oils on L. monocytogenes using disc diffusion and minimum inhibitory concentration (MIC) methods and then on initial cell attachment and six hours preformed biofilms. The inhibition of biofilms was assessed by crystal violet assay. Sulfides were the most abundant compounds present in onion and garlic essential oils, while cinnamaldehyde was predominant in cinnamon essential oil. MIC values were of 0.025 mg mL−1 for onion essential oil and 0.100 mg mL−1 for cinnamon and garlic. Onion essential oil inhibited initial cell attachment by 77% at 0.5 of the MIC dose, while at MIC, cinnamon and garlic essential oils inhibited the initial microbial adhesion completely. All three essential oils completely inhibited initial cell attachment when applied at 2 MIC. On the contrary, preformed biofilms were more resistant, and the inhibition rate ranged from 33% to 78%. In summary, this investigation revealed that the essential oils of garlic, onion, and cinnamon show an effective antibiofilm activity against L. monocytogenes and are promising natural antimicrobial alternatives for food processing facilities.

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Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics

Natural Product Communications ◽

10.1177/1934578x1801300325 ◽

2018 ◽

Vol 13 (3) ◽

pp. 1934578X1801300 ◽

Cited By ~ 2

Author(s):

Marina Milenković ◽

Jelena Stošović ◽

Violeta Slavkovska

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Broth Microdilution ◽

Bacterial Strains ◽

Microdilution Method ◽

Broth Microdilution Method ◽

The Subject

The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone ( C. sylvatica), pulegone and menthone ( C. vardarensis), pulegone and piperitenone ( C. nepeta), pulegone, piperitenone, menthone and piperitone ( C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 μg/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI ≤ 0.5) to antagonistic (FICI ≥ 2) depending on the bacterial strain tested.

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CHEMICAL COMPOSITION, ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS AND LEAF EXTRACTS OF MENTHA PULEGIUM (L) AND GLYCYRRHIZA FOETIDA (DESF) AGAINST THE PHYTOPATHOGENIC BACTERIA

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.031.01.0497 ◽

2019 ◽

Vol 31 (1) ◽

pp. 47-54

Author(s):

Hanane Lougraimzi ◽

Aghraz Abdellah ◽

Bouaichi Abdelaaziz ◽

El J. Meryem ◽

Achbani E. Hassan

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Minimal Bactericidal Concentration ◽

Mentha Pulegium ◽

Leaf Extracts ◽

Phytopathogenic Bacteria

This research was conducted to evaluate the components of the Mentha pulegium (L) and Glycyrrhiza foetida (Desf) that were extracted by hydro distillation and analyzed using GC-MS methods. They are spontaneous plants widespread in Gharb of Morocco. The aim of the study is to investigate the antibacterial activity of the essential oils and leaf methanolic extracts of these two medicinal plants against five phytopathogenic bacteria. The evaluation of the antimicrobial activity of the two species by estimating the diameter of the inhibition zone has shown that the essential oil of M. pulegium exhibited a higher antimicrobial activity than G. foetida which varied according to the sensitivity of the phytopathogenic strains. The results obtained revealed different degrees of sensitivity toward methnolic extract. However, the methanolic extract and the essential oil of M. pulegium are more active based on the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The antimicrobial activities of leaf extracts from the two plants highlighted in this study could justify their therapeutic use. These results should be strongly recommended as an alternative to a chemical compound that still represents the problem of toxic residues.

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Chemical Composition and Antimicrobial Activity of Marsdenia Koi Tsiang Essential Oil

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.713-715.2614 ◽

2015 ◽

Vol 713-715 ◽

pp. 2614-2616 ◽

Cited By ~ 1

Author(s):

Yong Min ◽

Ze Feng Wang ◽

Ai Ping Fang ◽

Li Zhang ◽

Chun Xu

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils

The present study is the first investigation of the chemical composition and antimicrobial activitys of Marsdenia koi Tsiang essential oils. A total of forty-five compounds were identified, representing about 94.9% of the studied oil.The essential oil exhibited antibacterial activity against B.subtilis, S.aureus and S. albus with the MIC values of 50 μg/ml, 100μg/ml and 100μg/ml respectively.

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Mosquito Larvicidal Activity, Antimicrobial Activity, and Chemical Compositions of Essential Oils from Four Species of Myrtaceae from Central Vietnam

Plants ◽

10.3390/plants9040544 ◽

2020 ◽

Vol 9 (4) ◽

pp. 544 ◽

Cited By ~ 7

Author(s):

Nguyen Thi Giang An ◽

Le Thi Huong ◽

Prabodh Satyal ◽

Thieu Anh Tai ◽

Do Ngoc Dai ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Larvicidal Activity ◽

Antimicrobial Agents ◽

Chemical Compositions ◽

Mosquito Larvicidal Activity ◽

Good Antibacterial Activity ◽

Central Vietnam

Mosquitoes are important vectors of several diseases, and control of these insects is imperative for human health. Insecticides have proven useful in controlling mosquito populations, but insecticide resistance and environmental concerns are increasing. Additionally, emerging and re-emerging microbial infections are problematic. Essential oils have been shown to be promising mosquito larvicidal agents as well as antimicrobial agents. In this work, the essential oils from four species of Myrtaceae (Baeckea frutescens, Callistemon citrinus, Melaleuca leucadendra, and Syzygium nervosum) growing wild in central Vietnam have been obtained by hydrodistillation and analyzed by gas chromatographic techniques. The essential oils have been screened for mosquito larvicidal activity against Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus, and for antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. Callistemon citrinus fruit essential oil, rich in α-pinene (35.1%), 1,8-cineole (32.4%), limonene (8.2%), and α-terpineol (5.8%) showed good larvicidal activity with 24-h LC50 = 17.3 μg/mL against both Ae. aegypti and Cx. quinquefasciatus, and good antibacterial activity against E. faecalis (minimum inhibitory concentration (MIC) = 16 μg/mL) The 48-h larvicidal activities of M. leucadendra leaf essential oil, rich in α-eudesmol (17.6%), guaiol (10.9%), linalool (5.1%), (E)-caryophyllene (7.0%), and bulnesol (3.6%) were particularly notable, with LC50 of 1.4 and 1.8 μg/mL on Ae. aegypti and Cx. quinquefasciatus. Similarly, M. leucadendra bark essential oil, with α-eudesmol (24.1%) and guaiol (11.3%), showed good antibacterial activity against. E. faecalis. Both B. frutescens and C. citrinus leaf essential oils demonstrated anti-Candida activities with MIC values of 16 μg/mL. The results of this investigation suggest that essential oils derived from the Myrtaceae may serve as “green” alternatives for the control of mosquitoes and/or complementary antimicrobial agents.

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Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

Italian Journal of Food Safety ◽

10.4081/ijfs.2016.6176 ◽

2016 ◽

Vol 5 (4) ◽

Cited By ~ 4

Author(s):

Stefania M. Marotta ◽

Filippo Giarratana ◽

Alessio Parco ◽

Domenico Neri ◽

Graziella Ziino ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Listeria Monocytogenes ◽

Essential Oils ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Strong Activity ◽

Agar Disc ◽

Inhibition Experiment

Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight Listeria monocytogenes strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for L. monocytogenes strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P<0.01), rather than the BEO employed (P>0.01). In conclusion, we can consider BEO as a natural technological hurdle for Listeria monocytogenes in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

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