Characterization of proteinuria in treated and untreated dogs naturally infected with Leishmania sp.

ABSTRACT: In the search for an early biomarker of renal injury, this study aimed to determine the urinary protein profile of dogs with leishmaniasis without treatment and treated as determined by Brazilian legislation. The identification of proteinuria, its classification and the circumstances in which it takes place instigated this study. For this, 30 dogs from an outpatient clinic at a Veterinary Hospital in Belo Horizonte were evaluated. All animals underwent clinical and laboratory tests, which included renal biomarkers. The proteins were characterized using the SDS-page electrophoresis technique, and thus, a urinary protein profile was developed comparing patients considered clinically healthy with dogs infected with leishmaniasis that were under treatment and with untreated infected dogs. The results showed that the hematological and biochemical parameters showed similar behavior between the groups of healthy dogs and dogs with leishmaniasis treated, however a very heterogeneous pattern of urinary proteins can be observed and differed between healthy animals and animals with leishmaniasis, as well as between treated and untreated animals. The results suggest that the classification of proteinuria can be a tool that helps in the staging of animals infected with L. infantum and can differentiate them as to the severity of existing kidney injuries.

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Morphology, Protein Profile and the Level of Infestation of Bedbugs (Hempitera: Cimicidae) in the Halls of Residence of a Higher Institution in Lagos.

NIGERIAN ANNALS OF PURE AND APPLIED SCIENCES ◽

10.46912/napas.70 ◽

2019 ◽

Vol 1 ◽

pp. 96-104

Author(s):

K A Kemabonta ◽

M O Ajiboye

Keyword(s):

Staining Method ◽

Profile Analysis ◽

Protein Profile ◽

Polyacrylamide Gel Electrophoresis ◽

Morphological Characterization ◽

Sds Page ◽

Higher Institution ◽

Cimex Hemipterus ◽

Very High

Morphology, protein profile and the level of Bedbug infestations were carried out at a University in Lagos, Nigeria after an outcry of high infestation by the students in halls of residence. Three bedbugswere each collected from nine halls of residence for morphological characterization of adults and to carry out protein profile analysis usingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie staining method. Level of infestation of Bedbugs was carried out in two female halls A and Busing blood smeared on the walls. All bedbugs found were Cimex hemipterus. 29.8% of the rooms in Hall Bhad no bedbug infestation, 23.9%, 20.2%, 15.4% and 10.6% had low, average, high and very high levels of bedbug infestation respectively while in A hall, it was 21.4%, 30.5%, 24.4%, 14.5% and 9.2% respectively. In A hall, 4.6%, 16.8% and 0.8% had one, two and three out of the four mattresses in the room infested with bedbugs respectively while 56.5% had all the four mattresses infested with bedbugs. In B hall, 1.6%, 16.0% and 0% had one, two and three mattresses infested with bedbugs respectively while the remaining 52.7% had all the four mattresses in the room infested with bedbugs. There was a significant relationship between the level of infestation and the number of mattresses infested in each hall (P < 0.01).The protein profile analysis of bedbugs did not show the protein bands clearly because of the low soluble protein content of Cimex hemipterus and the detection limit of Coomassie stain.

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Characterization of urinary protein profile in regular kratom (Mitragyna speciosa korth.) users in Malaysia

Journal of Addictive Diseases ◽

10.1080/10550887.2021.1981122 ◽

2021 ◽

pp. 1-12

Author(s):

Rana Khudhair Jasim ◽

Zurina Hassan ◽

Darshan Singh ◽

Edward Boyer ◽

Lay-Harn Gam

Keyword(s):

Protein Profile ◽

Urinary Protein ◽

Mitragyna Speciosa

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Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period

Biochemical Journal ◽

10.1042/bj2690265 ◽

1990 ◽

Vol 269 (1) ◽

pp. 265-268 ◽

Cited By ~ 80

Author(s):

P Nyirkos ◽

E E Golds

Keyword(s):

Synovial Cell ◽

Lung Fibroblasts ◽

Synovial Cells ◽

Terminal Sequence ◽

Reverse Phase ◽

Cell Monolayers ◽

Human Synovial Cell ◽

Sds Page

By SDS/PAGE analysis we have observed that human synovial cell monolayers secrete a prominent 39 kDa protein which could not be detected in skin and lung fibroblasts. This protein was purified to homogeneity by heparin-Sepharose chromatography and reverse-phase h.p.l.c. The N-terminal sequence was found to be almost identical to that of a recently described bovine protein detected in the mammary secretions during the involutionary phase of the lactational cycle. Characterization of this 39 kDa protein may provide a useful marker for classification of connective tissue cells.

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Characterization of enterotoxin producing Clostridium perfringens isolated from foods of animal origin

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.9167 ◽

2017 ◽

Cited By ~ 1

Author(s):

Shahnaz Haque ◽

Rajeev Kumar Sharma ◽

Nagendra Nath Barman ◽

Laishram Babita Devi ◽

Luit Moni Barkalita ◽

...

Keyword(s):

Clostridium Perfringens ◽

Protein Profile ◽

Meat Products ◽

Type A ◽

Animal Origin ◽

Cytopathic Effects ◽

Vero Cell Line ◽

Sds Page ◽

Type C

Screening of 105 samples comprising of milk, meat and their products for isolation of Clostridium perfringens revealed a total of 39 (37.14%) samples to be positive for C. perfringens, yielding an equal number of isolates. Among the total isolates, 11 (20.0%) belonged to milk and milk products, of which 10 were isolated from raw cow’s milk. The remaining isolate was from paneer sample. Similarly, 28 (56.0%) C. perfringens isolates were recovered from meat and meat products. Majority (37) of the isolates belonged to toxin type A while two isolates recovered each from raw beef and chicken meats were of toxin type C. Among the 39 isolates of C. perfringens, only 5(12.82%) isolates could reveal the presence of cpe (enterotoxin) gene. The protein profile (SDS-PAGE) of both the crude and partially purified enterotoxins prepared from cpe positive C. perfringens isolates (type A and C) revealed a very little difference. Both the partially purified enterotoxins of type A and C of C. perfringens displayed almost similar type of cytopathic effects (CPE) and death of cells in Vero cell line.

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Characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v49.i4.p213-216 ◽

2016 ◽

Vol 49 (4) ◽

pp. 213

Author(s):

Deby Kania Tri Putri ◽

Indah Listiana Kriswandini ◽

Muhammad Luthfi

Keyword(s):

Molecular Weight ◽

Dental Caries ◽

Streptococcus Mutans ◽

Profile Analysis ◽

Protein Profile ◽

Tooth Surface ◽

Molecular Receptors ◽

Streptococcus Sanguis ◽

Sds Page

Background: Dental caries is a major problem in oral cavity. If dental caries causes cavity, the structure of dental hard tissue will not be reversible because of damage in the structure of the hard tissue. The early pathogenesis mechanism of dental caries is an adhesion interaction between cariogenic Streptococcus mutans microorganisms and tooth surface pellicles. The attachment involves a specific molecular component interaction between the bacterial complement molecules and the surface of the host. Streptococcus sanguis as a dominant ecology at the beginning of bacterial plaque aggregation will colonize the tooth surface earlier than S. mutans. The surface of bacterial cells can express some adesin. The bacteria also can express receptors for adhesins of other bacteria. Specific receptors for adhesions of S. Mutans bacteria are not only found in the pellicles, but also present in pioneer bacteria, such as S. sanguis. Adhesion between those bacteria is called as coagregation. Purpose: This study aimed to analyze the characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules. Method: This study used a sonication method for protein isolation of S. mutans and S. sanguis bacterial biofilms, as well as electrophoresis method using 12 % SDS-PAGE gel and Western Blot analysis. Result: Results of the protein profile analysis of S. mutans biofilms using 12% SDS-PAGE showed that there were 17 bands, each of which molecular weights was 212, 140, 81, 65, 61, 48, 45, 44, 40, 39, 33 , 25, 23, 19, 17, 12, and 11 kDa. On the other hand, results of the protein profile analysis of S. sanguis biofilms using 12% SDS-PAGE showed that there were 15 bands, each of which molecular weight was 130, 85, 65, 61, 48, 46, 40, 37, 29, 25, 23, 21, 17, 15, and 12 kDa. And, results of the analysis of S. sanguis receptor molecules using Western blot showed that there were three bands, each of which molecular weight was 130, 85, and 40 kDa. Conclusion: S. sanguis bacteria have specific receptor molecules for S. mutans bacteria with the molecular weight of 130, 85, and 40 kDa.

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442 PB 247 BIOCHEMICAL CHARACTERIZATION OF CULTIVATED OPUNTIA SPECIES

HortScience ◽

10.21273/hortsci.29.5.494e ◽

1994 ◽

Vol 29 (5) ◽

pp. 494e-494

Author(s):

J.O. Kuti ◽

C.M. Galloway

Keyword(s):

Biochemical Characterization ◽

Protein Profile ◽

Seed Proteins ◽

Spectrophotometric Analysis ◽

Protein Profiles ◽

Genetic Studies ◽

Banding Patterns ◽

Molecular Weights ◽

Sds Page

The use of protein profiles and isozyme banding patterns as genetic markers in cultivated Opuntia species was investigated using SDS-PAGE and spectrophotometric analysis of seeds and stem (cladode) tissues. Twenty morphologically different entries belonging to six Opuntia species were analyzed for total protein profile and three enzyme systems (superoxide dismustase [SOD], phosphoglucomutase [PGM] and UDPG ppase). Seed proteins, mostly low molecular weights, were 3-fold that of cladode proteins. Marked differences in protein molecular weight were found among the entries. PGM activity, found only in the cladode tissues, differred among the entries. No UDPG ppase activity was found in either seeds or cladode tissues. Within the entries surveyed, identical SOD banding patterns were observed indicating some degree of similarity among the species. The preliminary results suggest that isozyme and protein profiles can be used as markers in genetic studies of cultivated Opuntia species.

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Characterization of Venoms of Deinagkistrodon acutus and Bungarus multicinctus Using Proteomics and Peptidomics

Current Proteomics ◽

10.2174/1570164617666191121112319 ◽

2020 ◽

Vol 17 (3) ◽

pp. 241-254

Author(s):

Yaqiong Zhang ◽

Zhiping Jia ◽

Yunyang Liu ◽

Xinwen Zhou ◽

Yi Kong

Keyword(s):

Snake Venom ◽

Protein Families ◽

Traditional Medicines ◽

Phospholipases A2 ◽

Inhibitory Peptides ◽

Sds Page ◽

Deinagkistrodon Acutus ◽

Venom Proteins ◽

Proteins And Peptides

Background: Deinagkistrodon acutus (D. acutus) and Bungarus multicinctus (B. multicinctus) as traditional medicines have been used for hundreds of years in China. The venoms of these two species have strong toxicity on the victims. Objective: The objective of this study is to reveal the profile of venom proteins and peptides of D. acutus and B. multicinctus. Method: Ultrafiltration, SDS-PAGE coupled with in-gel tryptic digestion and Liquid Chromatography- Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS/MS) were used to characterize proteins and peptides of venoms of D. acutus and B. multicinctus. Results: In the D. acutus venom, 67 proteins (16 protein families) were identified, and snake venom metalloproteinases (SVMPs, 38.0%) and snake venom C-type lectins (snaclecs, 36.7%) were dominated proteins. In the B. multicinctus venom, 47 proteins (15 protein families) were identified, and three-finger toxins (3FTxs, 36.3%) and Kunitz-type Serine Protease Inhibitors (KSPIs, 32.8%) were major components. In addition, both venoms contained small amounts of other proteins, such as Snake Venom Serine Proteinases (SVSPs), Phospholipases A2 (PLA2s), Cysteine-Rich Secreted Proteins (CRISPs), 5'nucleotidases (5'NUCs), Phospholipases B (PLBs), Phosphodiesterases (PDEs), Phospholipase A2 Inhibitors (PLIs), Dipeptidyl Peptidases IV (DPP IVs), L-amino Acid Oxidases (LAAOs) and Angiotensin-Converting Enzymes (ACEs). Each venom also had its unique proteins, Nerve Growth Factors (NGFs) and Hyaluronidases (HYs) in D. acutus, and Cobra Venom Factors (CVFs) in B. multicinctus. In the peptidomics, 1543 and 250 peptides were identified in the venoms of D. acutus and B. multicinctus, respectively. Some peptides showed high similarity with neuropeptides, ACE inhibitory peptides, Bradykinin- Potentiating Peptides (BPPs), LAAOs and movement related peptides. Conclusion: Characterization of venom proteins and peptides of D. acutus and B. multicinctus will be helpful for the treatment of envenomation and drug discovery.

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Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19980434 ◽

1998 ◽

Vol 63 (3) ◽

pp. 434-440 ◽

Cited By ~ 2

Author(s):

Irena Hulová ◽

Jana Barthová ◽

Helena Ryšlavá ◽

Václav Kašička

Keyword(s):

Concanavalin A ◽

Reversed Phase ◽

Amino Acid Sequences ◽

Gel Chromatography ◽

Affinity Ligand ◽

Sds Page ◽

Terminal Amino ◽

Α And Β Subunits

Glycoproteins that have affinity to Concanavalin A were isolated from the acetone-dried pituitaries of common carp (Cyprinus carpio L.). Two fractions of glycoproteins were separated using gel chromatography on Superdex 75HR. The fraction with lower molecular weight (30 000) corresponding to the carp gonadotropin cGtH II was composed of two subunits as determined using SDS-PAGE. This protein fraction was further divided into four components using reversed-phase HPLC. Two fractions were pure α and β subunits of cGtH II as follows from immunodetection and from determination of N-terminal amino acid sequences. The other two were a mixture of α and β subunits as was also revealed by N-terminal analysis. Capillary electrophoresis was also used for characterization of isolated glycoproteins.

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Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Insects ◽

10.3390/insects12070640 ◽

2021 ◽

Vol 12 (7) ◽

pp. 640

Author(s):

Natalia R. Moyetta ◽

Fabián O. Ramos ◽

Jimena Leyria ◽

Lilián E. Canavoso ◽

Leonardo L. Fruttero

Keyword(s):

Cell Biology ◽

Cell Types ◽

Transmission Electron ◽

Ultrastructural Characterization ◽

Current Classification ◽

Contrast Microscopy ◽

First Time ◽

Controversial Aspect

Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh preparations, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.

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Semi-automated regional classification of the style of activity of slow rock-slope deformations using PS InSAR and SqueeSAR velocity data

Landslides ◽

10.1007/s10346-021-01654-0 ◽

2021 ◽

Author(s):

Chiara Crippa ◽

Elena Valbuzzi ◽

Paolo Frattini ◽

Giovanni B. Crosta ◽

Margherita C. Spreafico ◽

...

Keyword(s):

Rock Slope ◽

Progressive Failure ◽

Principal Component ◽

Cost Effective ◽

Displacement Rate ◽

Machine Learning Classification ◽

Management Perspective ◽

Alpine Environments

AbstractLarge slow rock-slope deformations, including deep-seated gravitational slope deformations and large landslides, are widespread in alpine environments. They develop over thousands of years by progressive failure, resulting in slow movements that impact infrastructures and can eventually evolve into catastrophic rockslides. A robust characterization of their style of activity is thus required in a risk management perspective. We combine an original inventory of slow rock-slope deformations with different PS-InSAR and SqueeSAR datasets to develop a novel, semi-automated approach to characterize and classify 208 slow rock-slope deformations in Lombardia (Italian Central Alps) based on their displacement rate, kinematics, heterogeneity and morphometric expression. Through a peak analysis of displacement rate distributions, we characterize the segmentation of mapped landslides and highlight the occurrence of nested sectors with differential activity and displacement rates. Combining 2D decomposition of InSAR velocity vectors and machine learning classification, we develop an automatic approach to characterize the kinematics of each landslide. Then, we sequentially combine principal component and K-medoids cluster analyses to identify groups of slow rock-slope deformations with consistent styles of activity. Our methodology is readily applicable to different landslide datasets and provides an objective and cost-effective support to land planning and the prioritization of local-scale studies aimed at granting safety and infrastructure integrity.

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