scholarly journals Phytobiotic Activity of Piper Auritum and Ocimum Basilicum on Avian E. Coli

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
E Aguilar-Urquizo ◽  
MF Itza-Ortiz ◽  
JR Sangines-Garcia ◽  
AT Pineiro-Vázquez ◽  
A Reyes-Ramirez ◽  
...  
Keyword(s):  
Ocimum Basilicum ◽  
E Coli ◽  
Piper Auritum

scholarly journals The effect of essential oil of basil (Ocimum basilicum L.) on UV-induced mutagenesis in Escherichia coli and Saccharomyces cerevisiae

2008 ◽  
Vol 60 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Jasna Stanojevic ◽  
Tanja Beric ◽  
Biljana Opacic ◽  
Branka Vukovic-Gacic ◽  
Draga Simic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Ocimum Basilicum ◽  
Major Constituent ◽  
Spontaneous Mutagenesis ◽  
E Coli ◽  
Antimutagenic Effect ◽  
Nucleotide Excision ◽  
Induced Mutagenesis

The antimutagenic potential of essential oil (EO) of basil (Ocimum basilicum L.) and its major constituent linalool were studied with the E. coli K12 and S.cerevisiae D7 assays. In the E. coli assay, EO and linalool inhibited UV-induced mutagenesis in a repair-proficient strain, but had no effect on spontaneous mutagenesis in repair-proficient, nucleotide excision repair-deficient, and mismatch-deficient strains. By testing participation of different mechanisms involved in antimutagenesis, it was concluded that the antimutagenic effect against UV-induced mutagenesis involved decrease of protein synthesis and cell proliferation which led to increased efficiency of nucleotide excision repair. An antimutagenic effect of basil derivatives in S. cerevisiae was not detected.

scholarly journals Atividade antimicrobiana de óleos essenciais em bactérias patogênicas de origem alimentar

2012 ◽  
Vol 14 (1) ◽  
pp. 57-67 ◽  
Author(s):  
C. Valeriano ◽  
R.H. Piccoli ◽  
M.G. Cardoso ◽  
E. Alves
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Enterica ◽  
Ocimum Basilicum ◽  
Mentha Piperita ◽  
Enterobacter Sakazakii ◽  
Cymbopogon Citratus ◽  
E Coli ◽  
Origanum Majorana

Objetivou-se identificar e quantificar os constituintes e avaliar a atividade antimicrobiana dos óleos essenciais de Mentha piperita, Cymbopogon citratus, Ocimum basilicum e Origanum majorana contra cepas de Escherichia coli enteropatogênica, Salmonella enterica Enteritidis, Listeria monocytogenes e Enterobacter sakazaki. A obtenção dos óleos essenciais foi realizada a partir de folhas secas, empregando-se a técnica de hidrodestilação e utilizando-se a aparelho de Clevenger modificado. A atividade antibacteriana dos óleos essenciais foi determinada pelo método de difusão em ágar. Observou-se que os óleos essenciais inibiram o crescimento bacteriano, mas a efetividade foi variada. Entre os óleos essenciais testados, M. piperita apresentou maior atividade antibacteriana para E. coli, (8.106 UA mL-1) quando comparada as demais bactérias, atividade moderada para Salmonella enterica Enteritidis e Enterobacter sakazakii (1.706 e 3.200 UA mL-1 respectivamente) e baixa atividade para Listeria monocytogenes (106,67 UA mL-1). Já óleo essencial de Cymbopogon citratus apresentou maior atividade antimicrobiana frente a E. coli (9.386 UA mL-1) e atividade moderada frente a Enterobacter sakazakii, Salmonella enterica Enteritidis e Listeria monocytogenes (2.773 UA mL-1 para ambas). Ocimum basilicum apresentou maior atividade antibacteriana frente E. coli e Enterobacter sakazakii (6.826 e 8.106 UA mL-1 respectivamente), moderada atividade frente a Salmonella enterica Enteritidis (1.600 UA mL-1) e não apresentou atividade frente a Listeria monocytogenes.Origanum majorana também foi testado neste estudo e apresentou maior atividade antimicrobiana frente E. coli (5.973 UA mL-1), atividade moderada para Salmonella enterica Enteritidis e Enterobacter sakazakii (1.706 e 2.346 UA mL-1 , respectivamente) e não apresentou atividade para Listeria monocytogenes.

The composition and antibacterial activity of essential oils in three Ocimum species growing in Romania

2013 ◽  
Vol 8 (6) ◽  
pp. 600-608 ◽  
Author(s):  
Marius Stefan ◽  
Maria Zamfirache ◽  
Claudia Padurariu ◽  
Elena Trută ◽  
Irina Gostin
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Glandular Hair ◽  
Ocimum Basilicum ◽  
Glandular Hairs ◽  
E Coli ◽  
Hair Morphology ◽  
Ocimum Species ◽  
Germacrene D ◽  
Main Components

AbstractIn this study the glandular hair morphology, chemical composition and antimicrobial activity of the essential oils from three Ocimum species have been investigated (Ocimum basilicum L. var. Genovese, O. gratissimum and O. tenuiflorum). The indumentum shows little variation among the investigated species with both glandular and non-glandular hairs presents. Glandular hairs on the three species are peltate and capitate (with various cell numbers in the stalk and gland). The samples of essential oils obtained from the plant aerial organs by hydrodistillation have been analyzed by GC-MS. Linalool (65.38%, 74.22%, 38.60%), eugenol (5.26%, 3.47%, 10.20%) and tau-cadinol (8.18%, 3.47%, 10.20%) appear as the main components in Ocimum basilicum L. var. Genovese, O. gratissimum and O. tenuiflorum. The oils also contain lower levels of α-bergamotene, 1,8-cineole, germacrene D, β-ocimene, α-caryophyllene, camphor, and α-guaiene. All essential oils showed antibacterial activity against Staphylococcus aureus and Escherichia coli depending on their concentration. Ocimum basilicum L. var. Genovese oil produced the strongest antibacterial effect on S. aureus and E. coli.

scholarly journals ANGKA PALING MUNGKIN DAN DETEKSI COLIFORM PADA SAMPEL LALAPAN DAUN KEMANGI (Ocimum bacilicum) DI KOTA PONTIANAK

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ita Purnama Sari ◽  
Rahmawati Rahmawati ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Nutritional Value ◽  
Ocimum Basilicum ◽  
Coliform Bacteria ◽  
Processed Food ◽  
E Coli ◽  
Bacterial Genus ◽  
Maximum Limit ◽  
Food Consumed

Ocimum basilicum is one type of vegetables that can be consumed without having to be processed. Food consumed must have high nutritional value, clean and free of microbes. Coliform is one of the bacteria that can cause disease in humans. This study aims to determine the value of MPN on samples Ocimum basilicum leaves from warung lamongan in Pontianak and determine the existence of coliform bacteria on Ocimum basilicum leaf samples from warung lamongan in Pontianak. Detection of coliform bacteria using the MPN method. Detection of E. coli was carried out using EMBA media. The results showed that all samples had interval values between 210 MPN/g to > 1100 MPN/g. It showed that all samples excced the threshold determined by BPOM (2016), which was the maximum limit of coliform bacteria contamination in ready-toconsume vegetables was < 3 APM/g. The results of the identification of the bacterial genus coliform grouphad the members of genera Escherichia and Citrobacter

scholarly journals Antibacterial Potential of Essential Oils Against Ground Beef Isolates

2017 ◽  
Vol 24 (2) ◽  
pp. 161
Author(s):  
Lisiane Martins Volcão ◽  
Juliana de Lima Marques ◽  
Lucas Moreira dos Santos ◽  
Suzane Olachea Allend ◽  
Kamila da Cunha Furtado ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ground Beef ◽  
Ocimum Basilicum ◽  
Bacterial Activity ◽  
Cymbopogon Citratus ◽  
Salmonella Spp ◽  
Cinnamomum Zeylanicum ◽  
E Coli ◽  
Antibacterial Potential ◽  
Coagulase Positive Staphylococcus

The objective of this study was to evaluate the antibacterial activity of different EOs against Escherichia coli, Salmonella spp., and coagulase-positive staphylococcus isolated from ground beef. The EOs had values varying from 0.78μl/mL to 100μl/mL, and the essential oil of Citrus lemon did not present any bacterial activity against the studied strains. For E. coli, the essential oil of Cinnamomum zeylanicum presented the best inhibitory activity (MIC = 0.78μl/mL to 1.56μl/mL). In Salmonella spp., the EOs of Cymbopogon citratus (MIC = 12.5μl/mL and 25.0μl/mL), C. zeylanicum (MIC = 25.0 µL/mL) and Ocimum basilicum (MIC = 6.25μl/mL, 12.5μl/mL and 50.0μl/mL) exhibited similar results. The EOs of C. zeylanicum and Eugenia caryophyllata demonstrated the lowest average values of study against coagulase-positive Staphylococcus (MIC = 3.12μl/mL, 6.25μl/mL and 12.5μl/mL). With the data obtained in the study, it can be observed the potential of EOs in the control of pathogenic food microorganisms, as well as validate future researches on the proprieties and active compounds of these products, besides the toxicity of these compounds and the possible alterations that can be caused on the food.

scholarly journals Harinas de hojas de plantas aromáticas como fitoterapéuticos en pollos de engorda

2010 ◽  
Vol 45 (3) ◽  
pp. 294-298
Author(s):  
Pedro Enrique Lara y Lara ◽  
Mateo Fabian Itzá Ortiz ◽  
Edgar Aguilar Urquiso ◽  
José Roberto Sanginés García
Keyword(s):  
Ocimum Basilicum ◽  
Origanum Vulgare ◽  
Piper Auritum

El objetivo del presente trabajo fue evaluar el comportamiento productivo y el rendimiento de la canal en pollos de engorda, con el uso de harina de hojas de plantas aromáticas como aditivo fitoterapéutico al 0,07% en la dieta. Fueron utilizados 280 pollitos machos de la estirpe Ross 308, de 1 a 42 días de edad, distribuidos de forma aleatoria en un diseño completamente al azar, en cuatro tratamientos y siete repeticiones con 10 pollos cada una. Las combinaciones fueron en proporción 50:50 de harina de Origanum vulgare y Piper auritum (OHS), O. vulgare y Ocimum basilicum (OA), O. basilicum y P. auritum (HSA), y un testigo con flavomicina al 4%. El grupo testigo obtuvo el mayor peso corporal (2.385 g), consumo de alimento (204 g por ave por día) y mortalidad acumulada (21,87%), al final de la prueba. No hubo diferencias con la combinación de OA (2.198 g) y HSA (2.023 g) en peso corporal y consumo de alimento. La combinación OA registró la menor (1,96) y OHS la mayor (2,44) conversión de alimento. No se encontraron diferencias en el rendimiento de la canal. La combinación al 50% de O. vulgare y O. basilicum, incluida al 0,07% en la dieta para pollos de engorda, es una alternativa como promotora del crecimiento y no altera el rendimiento de la canal.

scholarly journals Atividade antibacteriana do óleo essencial do manjericão frente a sorogrupos de Escherichia coli enteropatogênica isolados de alfaces

2010 ◽  
Vol 40 (8) ◽  
pp. 1791-1796 ◽  
Author(s):  
André Gustavo Lima de Almeida Martins ◽  
Adenilde Ribeiro Nascimento ◽  
João Elias Mouchrek Filho ◽  
Nestor Everton Mendes Filho ◽  
Antonio Gouveia Souza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactuca Sativa ◽  
Ocimum Basilicum ◽  
E Coli

Considerando-se a crescente participação dos vegetais na transmissão de microrganismos, incluindo as Escherichia coli enteropatogênicas multirresistentes às drogas convencionais, a busca por novos compostos com propriedades antimicrobianas a partir de fontes naturais, tais como os óleos essenciais, tem se intensificado nos últimos anos. Esta pesquisa objetivou avaliar a atividade antibacteriana do óleo essencial do manjericão (Ocimum basilicum Linn.) frente a sorogrupos de Escherichia coli enteropatogênicas (EPEC) isolados de alfaces (Lactuca sativa), utilizando-se o Método de Difusão em Disco (MDD). Os resultados evidenciaram que todas as cepas de E. coli EPEC testadas apresentaram sensibilidade à ação do óleo essencial, sugerindo que este possa ser uma fonte de compostos com potencial terapêutico no combate a bactérias patogênicas.

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Ribosome Structural Organization

1974 ◽  
Vol 32 ◽  
pp. 332-333
Author(s):  
James A. Lake
Keyword(s):  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Small Subunit ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
Specific Antibodies ◽  
X Ray ◽  
E Coli ◽  
Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

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