scholarly journals Azospirillum brasilense inoculation methods in corn and sorghum

Author(s):  
Agner de Freitas Andrade ◽  
Tiago Zoz ◽  
André Zoz ◽  
Carlos Eduardo da Silva Oliveira ◽  
Travis Wilson Witt

ABSTRACT The Azospirillum brasilense inoculation is usually carried out via seed. However, this bacterium presents passive penetration in the plant, what makes it possible to inoculate the crops using other methods. This study aimed to evaluate the influence of A. brasilense inoculation methods on the emergence and initial growth of corn and sorghum, under greenhouse conditions. A randomized block design, with four replications, in a 4 x 2 factorial scheme, was used. The first factor consisted of a control treatment (without inoculation) and three inoculation methods: via seed, via sowing furrow and via leaf spray. The second factor was composed by two hybrids of corn and two of sorghum. The application of A. brasilense to the seed can reduce the emergence percentage and the emergence speed index of corn seedlings, depending on the genotype, but its application does not influence the initial growth of the plants. The A. brasilense inoculation via seed and via sowing furrow can reduce the emergence and increase the mean emergence time of sorghum seedlings, depending on the genotype. The A. brasilense inoculation via seed results in an increase in the dry mass of the root system of sorghum plants.

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Edson de Oliveira Santos ◽  
Adriana Miranda de Santana Arauco ◽  
Bruno de Oliveira Dias ◽  
Emanuel França Araújo ◽  
Cácio Luiz Boechat ◽  
...  

The use of renewable materials as a source of nutrients, besides being a solution of interest for waste disposal, may also be an effective way to reduce the high costs of inputs needed for the production of forestry seedlings. The purpose of this study was to evaluate the growth of Anadenanthera colubrina (Vell. Brenan) (angico branco) cultivated in organic substrates. A randomized block design was adopted and the treatments were distributed in a 3 × 5 factorial scheme, with three organic residues: decomposed burity (moriche palm) stem, carnaúba straw and organic compost mixed with the soil in four percentages: 20%, 40%, 60% and 80% of the composition of substrate and a control treatment with soil only, without incorporating the organic residue, with six repetitions. Ninety days after emergence, the seedlings were collected to determine the growth parameters: stem diameter (SD), height (H), of the following indexes: H/D, leaf number (LN), total chlorophyll (T), leaf area (LA), dry mass of the aerial part (DMAP), dry mass of roots (DMR), total dry mass (TDM) H/DMAP: DMAP/DMR, and Dickson Quality Index (DQI). The treatments that contained 60% and 80% of organic compost presented the best responses. It is concluded that the production of angico branco seedlings cultivated with substrate with the addition of 80:20 (organic compost: soil) presented the best results and was technically feasible according to the characteristics and indexes analyzed.


Author(s):  
Jorge Antonio Chávez Vergara ◽  
Antonio Torres García ◽  
Edixon Agustín Espinoza Vera ◽  
Diego Efrén Zambrano Pazmiño

Morphophysiological response of the rice root (Oryza sativa L.) variety SFL 11 in the seedbed phase to the application of the native strain of Trichoderma sp. and leached from bovine vermicompost Ecuador ocupa el lugar 26 a nivel mundial en la producción de arroz con un consumo percápita de 48 kilogramos anuales. En el país, Manabí es la tercera provincia, con 8 710 hectáreas sembradas y rendimiento promedio de 5,58 tha-1. El sistema radicular constituye la primera línea de defensa de las plantas, indispensable para la productividad de los cultivos. En la rizosfera se encuentran microorganismos benéficos, uno de ellos es el hongo Trichoderma, cosmopolita en suelos gracias a su gran plasticidad ecológica y caracterizado, entre otras funciones, por su efecto estimulador en el crecimiento vegetal al colonizar raíces aumentando los pelos radiculares y la extensión en profundidad de enraizamiento. Por otra parte, los bioestimulantes a base de sustancias húmicas promocionan el crecimiento radicular. El experimento se realizó para evaluar si hay inducción a repuestas morfofisiológicas en las raíces por una cepa nativa de Trichoderma sp. y el lixiviado de vermicompost bovino en el arroz (Oryza sativa L.) variedad SFL 11 en fase de semillero. La investigación se condujo en diseño de bloques completamente al azar con 8 tratamientos, seis con Trichoderma sp., uno con lixiviado de vermicompost bovino y el testigo con agua y se realizó en la parroquia Charapotó del cantón Sucre en la provincia de Manabí. A los 25 días después de la siembra fue determinado a las raíces la longitud (cm), volumen (mL) y masa seca (g) a un total de 20 plantas de las cuatro réplicas del experimento y los resultados sometidos a análisis de varianza simple y las medias comparadas mediante prueba de Duncan al 5%. El análisis estadístico demostró efecto benéfico significativo de los bioestimulantes Trichoderma sp., y el lixiviado de vermicompost bovino en la longitud y el volumen radicular, mientras que la biomasa seca demostró diferencias numéricas en crecimiento de las plantas de arroz (Oryza sativa L.) variedad SFL 11 en fase de semillero en comparación al tratamiento testigo. Palabras clave: Microorganismos benéficos; Trichoderma; bioestimulantes; rizósfera. Abstract Ecuador occupies the 26th place worldwide in the production of rice with a per capita consumption of 48 kilograms per year. In the country, Manabí is the third province, with 8 710 hectares planted and an average of 5.58 tha-1. The root system constitutes the first line of defense of the plants, necessary for the productivity of the crops. In the rhizosphere are specifically functional microorganisms, one of them is the fungus Trichoderma, cosmopolitan in soils thanks to its great ecological plasticity and characterized, among other functions, by its stimulating effect on plant growth by colonizing roots, increasing the root hairs and extension in depth of rooting. On the other hand, biostimulants based on vermicompost leachate promotes root growth. The experiment was carried out to evaluate if there is induction to morphophysiological responses in the roots by a native strain of Trichoderma sp. and the leaching of bovine vermicompost in rice (Oryza sativa L) variety SFL 11 in the seedling stage. The investigation was conducted in completely randomized block design with 8 treatments, six with Trichoderma sp., one with leachate of bovine vermicompost and the control with water and was carried out in the Charapoto parish of the Sucre canton in the province of Manabí. At 25 days after sowing the length (cm), volume (mL) and dry mass (g) were determined to the roots at a total of 20 plants of the four replicates of the experiment and the results subjected to simple variance analysis and the means compared by 5% Duncan test. Statistical analysis showed a significant beneficial effect of Trichoderma sp. Biostimulants, and leaching of bovine vermicompost in root length and volume, while dry biomass showed numerical differences in the growth of rice plants (Oryza sativa L) variety SFL 11 in the seedbed compared to the control treatment. Keywords: Beneficial microorganisms; Trichoderma; biostimulants; rhizospher.


Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 01 ◽  
Author(s):  
Gustavo Ribeiro Barzotto ◽  
Sebastião Ferreira Lima ◽  
Osvaldir Feliciano Santos ◽  
Gabriel Luiz Piati ◽  
Carlos Roberto Wassolowski

A cevada constitui uma importante cultura no Brasil devido, principalmente, a utilização de seu grão na produção de cervejas. O objetivo deste trabalho foi avaliar os efeitos da adubação nitrogenada e da inoculação com Azospirillum brasilense em cevada. O experimento foi instalado em campo, utilizando o delineamento em blocos casualizados em esquema fatorial, sendo quatro doses de nitrogênio (0, 40, 80 e 120 kg ha-1) na presença ou ausência de inoculação de sementes com A. brasilense e três repetições. Foram avaliadas características de crescimento e massa seca de planta, componentes de produção, teor de N e produtividade de grãos. A maior produtividade de grãos foi alcançada com 66 kg ha-1 de N, sem a aplicação da bactéria. Concluiu-se que o uso de A. brasilense aumenta a produtividade de grãos de cevada na ausência da adubação nitrogenada, mas não suplanta o ganho de produtividade apenas com o uso do N em doses superiores. A severidade de B. sorokidiana foi favorecida com a aplicação de A. brasilense nas maiores doses de N. A inoculação com A. brasilense é uma alternativa viável para aumentar a produtividade de grãos de cevada, caso se opte pelo cultivo sem fertilização mineral nitrogenada.Palavra-chave: Hordeum vulgare, bactérias diazotróficas, malte. NITROGEN FERTILIZATION AND INOCULATION WITH AZOSPIRILLUM BRASILENSE IN BARLEY ABSTRACT:The barley is an important crop in Brazil, mainly due to the use of its grain in the production of beers. The objective of this work was to evaluate the effects of nitrogen fertilization and inoculation with Azospirillum brasilense on barley. The experiment was carried out in the field, using a randomized block design in a factorial scheme, with four nitrogen doses (0, 40, 80 and 120 kg ha-1) in the presence or absence of seed inoculation with A. brasilense and three replications. Growth characteristics and dry mass of the plant, production components, N content and grain yield were evaluated. The highest grain yield was achieved with 66 kg ha-1 of N, without application of the bacteria. It was concluded that the use of A. brasilense increases the yield of barley grains in the absence of nitrogen fertilization, but does not supplant the productivity gain only with the use of N in higher doses. The B. sorokidiana severity was favored by the application of A. brasilense in the highest N doses. The inoculation with A. brasilense is a viable alternative to increase the yield of barley grains, in case of cultivation without nitrogen fertilization.Keywords: Hordeum vulgare, diazotrophic bacteria, malt. DOI:


Author(s):  
Jaqueline de Araújo Barbosa ◽  
Ivonei Perego ◽  
Claudia Tochetto ◽  
Neumarcio Vilanova da Costa

A matocompetição limita o crescimento e o desenvolvimento da cultura do trigo. Porém, a inoculação com bactérias diazotróficas pode reduzir os efeitos da matocompetição. Este trabalho teve como objetivo avaliar a inoculação de Azospirillum brasilense em sementes de trigo, e seus efeitos nos estádios iniciais de desenvolvimento, sendo submetidas em convivência com plantas de azevém. Utilizou-se delineamento experimental em blocos casualizados, e esquema fatorial 2x3, com quatro repetições. O primeiro fator correspondeu a inoculação de sementes trigo com A. brasilense, e o segundo fator a três densidades populacionais de azevém (0, 25, 50). Avaliações foram realizadas nas plantas de trigo aos 18 e 34 dias após a emergência (DAE). Aos 18 DAE, na densidade de 50 plantas de azevém, as plantas de trigo inoculadas foram 30% maiores em altura. Houve redução no número de folhas, em que as plantas de trigo inoculadas apresentaram número de folhas 33% maior do que as não inoculadas, além de índice de SPAD 23% maior. Aos 34 DAE, na densidade de 50 plantas de azevém, houve aumento de 21% na altura das plantas de trigo, índice SPAD 13% maior, e área foliar 15% maior em relação às plantas não inoculadas. Apesar do aumento de 68% no acúmulo de massa seca, as plantas inoculadas não diferiram em biomassa em relação à testemunha, quando em matocompetição. Conclui-se que a inoculação com A. brasilense favorece a cultura do trigo em matocompetição até os 18 DAE, mas ao final de 34 DAE, não se observam efeitos satisfatórios. Palavras-chave: Diazotróficas. Lolium multiflorum. Matocompetição. Plantas Daninhas. AbstractThe weed competition limits the growth and development of the wheat crop. However, the inoculation with diazotrophic bacteria can reduce the effects of weed competition. The objective of this work was to evaluate the inoculation with Azospirillum brasilense in seeds, and its effects in the early stages of development, and subjected to  coexistence with plants of azevém.  A completely randomized block design, and factorial 2x3, with four replications were used. The first factor corresponded to wheat seed inoculation with A. brasilense, and the second factor to three population densities of azevém (0, 25, 50). Evaluations were performed in wheat plants at 18 and 34 days after emergence (DAE). At 18 DAE, the density of 50 plants of azevém, the wheat plants inoculated were 30% greater in height. There was a reduction in the number of leaves, in which the wheat plants inoculated presented a number of sheets 33% higher than those not inoculated, in addition to SPAD index 23% higher. At 34 DAE, the density of 50 plants of azevém, there was an increase of 21% in the height of wheat plants, SPAD index 13% higher leaf area, and 15% higher than the non-inoculated plants. Despite the increase of 68% in the accumulation of dry mass, the inoculated plants did not differ in biomass in relation to the control , when in weed competition.  It is concluded that inoculation with A. brasilense favors wheat cultivation in weed competition up to 18 DAE, but at the end of 34 DAE, no satisfactory effects are observed. Keywords: Diazotrophic. Lolium multiflorum. Matocompetition. Weeds.  


2021 ◽  
Vol 6 ◽  
pp. 1-9
Author(s):  
Roberto Takane ◽  
Cyro Fragoso Silva ◽  
Jessica Soares Pereira ◽  
Christian Morimasa Takemura ◽  
Talita Angélica ◽  
...  

The present work aimed to evaluate the influence of use, as well as to determine adequate doses in organic fertilization of the bokashi type in the cultivation of two basil (Ocimum basilicum L.) cultivars. Seeds from the collection of the Floriculture Laboratory of the Phytotechnics Department of the Federal University of Ceará were used. The treatments used were arranged in a randomized block design (RBD), arranged in a 2x5 factorial scheme, consisting of two cultivars of basil (Genovese and Anão) and five doses of Bokashi, namely: 0, 3, 6, 9 and 18 g.L-1. Plants that received no dose (0 g.L-1) served as a control treatment. Each treatment contained 5 repetitions, with the plot consisting of 7 pots, one plant per pot, the useful unit a pot with a basil seedling. From the transplant, applications of bokashi were started in doses of 0, 3, 6, 9, and 18 g.L-1 in both cultivars. Both applications were performed every 20 days after the first application of Bokashi. Fivety five days after transplantation in the final pot, the following variables were analyzed: plant height, stem diameter, fresh and dry mass of the aerial part and the root. The 9 g.L-1 dose of Bokashi showed the best results for the variables analyzed for both cultivars, in addition to being efficient when compared to the control, in which the vegetative growth was incipient.


2014 ◽  
Vol 18 (1) ◽  
pp. 85-89 ◽  
Author(s):  
Jorge L. da Paixão Filho ◽  
Giuliano Gabrielli ◽  
Bruno Coraucci Filho ◽  
Adriano L. Tonetti

One option for the increasing production of sewage sludge (SS) is its agricultural use in crops with high nutritional demand, such as the rose. Therefore, the aim of this work was to study the application of SS from primary stabilization pond in roses of the variety 'Carola'. For the experiment, the roses were planted in 20 L recipients using increasing doses of sludge (T2 - 12 Mg ha-1, T3 - 24 Mg ha-1 and T4 - 36 Mg ha-1), mineral fertilizer (T5) and control treatment (T1, without N) in a randomized block design. The evaluated parameters were: roots dry mass, aerial dry mass, total biomass, leaflets area and nitrogen concentration in the leaf. The SS application resulted in better development of the rose, increasing the roots and the aerial biomasses, the leaflets area, and nitrogen content in the leaves compared to the control (T1). There was no statistical difference between the use of mineral fertilizer (T5), and the SS under the highest dose (T4). With these benefits, the use in agriculture of sewage sludge derived from stabilization ponds proved to be an adequate option for its disposal.


2019 ◽  
Vol 32 (4) ◽  
pp. 986-994
Author(s):  
JANAINA BORGES DE AZEVEDO FRANÇA ◽  
FREDERICO ANTONIO LOUREIRO SOARES ◽  
NEI PEIXOTO ◽  
MUZA DO CARMO VIEIRA ◽  
ANDERSON DIAS VAZ DE SOUZA

ABSTRACT The use of cultivars appropriate to the soil and climate characteristics of a region provides farmers with security by facilitating credit for crop financing and improving product prices in local commerce. The objective of this work was to evaluate the agronomic performance of “Contender” and “Amarelo Japonês” green bean bush cultivars grown in the Cerrado in terms of water replenishment (RH) of 25, 50, 75, 100, and 125% of evaporation using the Piché evaporimeter. The experiment was carried out in the experimental area of the State University of Goiás, on the Ipameri Campus. The experimental design was a randomized block design. Sowing was done in May 2017, with treatments distributed in a split-plots scheme with water replenishment in plots and cultivar subplots with 3 replications. The analyzed variables were: height; stem diameter; leaf number; leaf area; number of inflorescences; number of flowers; fresh and dry mass of the plant; number, length, diameter, fresh and dry mass of the pods. The different water replenishment rates increased the mean plant height, stem diameter, number of pods per plant, number of bean seeds per pod, and number of bean seeds per plant in the cultivars “Amarelo Japonês” and “Contender.” Water replenishment of 125% produced higher development and better agronomic performance in the two cultivars than the other rates.


2019 ◽  
Vol 11 (5) ◽  
pp. 454
Author(s):  
Fábio Oseias dos Reis Silva ◽  
José Darlan Ramos ◽  
Ranieri Reis Laredo ◽  
Verônica Andrade dos Santos ◽  
Nathalia Vallery Tostes ◽  
...  

The fruit plant ‘Longan’ is considered as being native to Asia and is at present commercially explored in some regions. The objective of this work was to study the influence of seed size on seed emergence and initial seedling growth. The experiment was carried out using a randomized block design (DBC), with three treatments, namely, the seed sizes at seedling emergence according to their longitudinal diameter: small, medium and large. Each plot was composed of six seeds and five replications per treatment. The following characteristics were evaluated: emergence speed index, average time, percentage and relative frequency of emergence, leaf number, stem diameter, plant height, shoot dry mass and root dry mass. Seeds of medium and large size are those that presented satisfactory results for the characteristics related to seed emergence. The same ones stood out in relation to the small seeds for the variables percentage, average time, average speed and emergence speed index. Small seeds are not recommended for the production of ‘Longan’ seedlings, given the long period of time to emerge and the low probability of the seedlings establishing satisfactorily in the field. The medium and large seeds were the most promising for the variables number of leaves, stem diameter, plant height, dry shoot mass and dry root mass.Therefore, according to the conditions in which the present work was conducted, the use of medium and large seeds for the production of ‘Longan’ seedlings is suggested.


2020 ◽  
Vol 19 ◽  
pp. 17
Author(s):  
SUZETE FERNANDES LIMA ◽  
LEANDRO SPÍNDOLA PEREIRA ◽  
GUSTAVO SILVA DE OLIVEIRA ◽  
GUSTAVO DORNELAS DE SOUSA ◽  
PAULO CÉSAR TIMOSSI ◽  
...  

Evaluation was carried out for the interrelation between maize and Urochloa brizantha cv. Marandu and Urochloa ruziziensis, with and without application of glyphosate underdoses, and the effects of this management on weeds. For each forage species, an experiment was conducted in randomized block design, with subdivided plots. The main factor consisted of four cropping systems: intercropping of maize and U. brizantha, treated with 0, 50 and 100 g a.e. ha-1 of glyphosate, and maize monoculture; and for the intercropping of maize and U. ruziziensis, the doses of glyphosate applied were 25 and 50 g a.e. ha-1. The secondary factor consisted of five evaluation periods, with dry mass of maize and forage plants being measured on the day when the herbicide was applied, 15 days after the application, at full tasseling of maize, in hard farinaceous grain stage, and at maize harvest. Weed density and dry mass were also evaluated. The intercropping system reduced the density and dry mass production of the weed community, without affecting the maize yield components. Glyphosate suppressed the initial growth of U. brizantha and U. ruziziensis intercropped with maize, at the dose of 100 and 50 g a.e. ha-1, respectively, without compromising straw (residues) formation.


2012 ◽  
Vol 34 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Ana Cristina Vello Loyola Dantas ◽  
João Mariano de Oliveira Queiroz ◽  
Elvis Lima Vieira ◽  
Vanessa de Oliveira Almeida

Plant growth regulators and biostimulants have been used as an agronomic technique to optimize the production of seedlings in various crops. This study aimed to evaluate the influence of gibberellic acid and the biostimulant Stimulate® on the initial growth of tamarind (Tamarindus indica L.). The experiments were conducted in a nursery with 50% shading, in a randomized block design with five replications and five plants per plot. Thirty eight days after sowing, the leaves were sprayed seven times a day with 0.0 (control), 0.8, 1.6, 2.4 and 3.2 mL of gibberellic acid L-1 aqueous solution and with 0.0 (control), 6.0,12.0, 18.0, and 24.0 mL Stimulate® L-1 aqueous solution. Stem diameter (SD), plant height (PH), longest root length (LRL), shoot dry mass (SDM), root dry mass (RDM) and RDM:SDM ratio were evaluated ninety days after sowing. Variance and regression analysis showed that GA3 at 4% promoted plant growth (height), but had no significant effect on stem diameter, longest root length, shoot and root dry mass and the RDM:SDM ratio. On the other hand, all concentrations of Stimulate® significantly increased plant height and shoot and root dry mass of tamarind seedlings.


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