scholarly journals Physiological and isozyme alterations in lettuce seeds under different conditions and storage periods

2016 ◽  
Vol 38 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Hugo César Rodrigues Moreira Catão ◽  
Luiz Antonio Augusto Gomes ◽  
Renato Mendes Guimarães ◽  
Pedro Henrique Faria Fonseca ◽  
Franciele Caixeta ◽  
...  
Keyword(s):  
Room Temperature ◽  
Physiological Changes ◽  
Lettuce Seeds ◽  
Randomized Design ◽  
Grand Rapids ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Storage ◽  
Different Levels

Abstract: The objective of the study was to determine the physiological and isozyme alterations in lettuce seeds submitted to different conditions and storage periods. We used a completely randomized design with four replications in a factorial arrangement with six lettuce cultivars (Everglades, Babá de Verão, Elisa, Grand Rapids, Hortência and Salinas 88) and four storage periods (30, 60, 90 and 120 days), on three storage rooms (15, 25 and 35 ° C). The physiological quality was evaluated by first and final germination count, emergence, and germination and emergence speed indexes. The isoenzyme expression of catalase (CAT), esterase (EST), alcohol dehydrogenase (ADH) and malate dehydrogenase (MDH) were determined. The physiological quality of lettuce seeds was preserved when stored at 15 ° C room temperature. The high room temperatures associated with higher storage periods cause a reduction in physiological quality and alterations in enzymatic patterns of CAT, EST, ADH and MDH. For seeds stored at 15 ° C, there were no physiological changes. The lettuce cultivars, especially Everglades, have different levels of tolerance to storage and isoenzyme expressions.

scholarly journals The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽  
2021 ◽  
Vol 5 (S2) ◽  
pp. 113-119
Author(s):  
W. Swastike ◽  
E. Suryanto ◽  
Rusman ◽  
C. Hanim ◽  
Jamhari ◽  
...  
Keyword(s):  
Peroxide Value ◽  
Room Temperature ◽  
Thiobarbituric Acid ◽  
Quality Properties ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

scholarly journals Seed quality of rice cultivars stored in different environments

2014 ◽  
Vol 36 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Elizabeth Rosemeire Marques ◽  
Eduardo Fontes Araújo ◽  
Roberto Fontes Araújo ◽  
Sebastião Martins Filho ◽  
Plínio César Soares
Keyword(s):  
Seed Quality ◽  
Accelerated Aging ◽  
Storage Period ◽  
Rice Cultivars ◽  
Germination Test ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Storage

The objective of this study was to evaluate dormancy and physiological quality of seeds of rice cultivars during storage in different environments. After harvesting, the seeds of three rice cultivars (Seleta, Curinga and Relâmpago) were dried in the sun, to reach moisture content at around 13%. Then, they were packed in paper and stored in four environments: 5 ± 2 ºC / 70 ± 5% RH, 12 ± 2 ºC / 70 ± 5% RH, 18 ± 2 ºC / 65 ± 5% RH and in uncontrolled condition of temperature and relative humidity (natural). Physiological quality was evaluated at the beginning and at 3, 6, 9 and 12 months of storage by germination test, electrical conductivity, accelerated aging and sand emergence. The experiment was conducted in split plots in a completely randomized design with three replications. Environmental factor was applied in the plots, cultivars in the subplots and storage period in subplots. Cultivar Seleta showed higher dormancy, which was surpassed during storage regardless of environment conservation. In general, seeds stored in natural environment showed lower physiological quality. Only the seeds of cultivar Seleta, regardless of the environment, maintained germination above the minimum required for commercialization until six months of storage.

scholarly journals Seed quality response of Urochloa humidicola cv. Llanero to drying surfaces and environments1

2018 ◽  
Vol 48 (3) ◽  
pp. 306-315
Author(s):  
Alan Mario Zuffo ◽  
Joacir Mario Zuffo Júnior ◽  
Everton Vinicius Zambiazzi ◽  
Fábio Steiner
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Seed Storage ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Quality Response ◽  
And Storage

ABSTRACT The drying process and storage may interfere in the quality of creeping signalgrass seeds [Urochloa humidicola (Rendle.) Morrone & Zuloago, syn. Brachiaria humidicola (Rendle.) Schweick.]. This study aimed to evaluate the physical, physiological and sanitary quality of creeping signalgrass cv. Llanero seeds submitted to drying surfaces and environments and stored for two periods (1 and 8 months). The experiment was arranged in a completely randomized design, in a 4 × 2 × 2 + 2 factorial arrangement, with four drying surfaces (soil, asphalt, concrete and ceramic floor), two drying environments (seeds exposed to the sun or shade), two seed storage periods (1 and 8 months) and two additional treatments with drying in paper bags under laboratory conditions (control) and stored for 1 or 8 months, with four replicates. The water content, viability by the tetrazolium test, electrical conductivity, germination rate, germination rate index and seed sanitary quality were measured. Drying at full sun impaired the physiological quality of the seeds due to the high temperatures (46.8-51.0 ºC) of the drying surface; however, reduced the incidence of pathogens. The drying of seeds in paper bags under shade results in a higher physiological quality, but the sanitary quality is compromised. However, the pathogens present in the seeds did not inhibit their viability and vigor. The storage for eight months reduces the seed quality.

scholarly journals Germination temperatures affect the physiological quality of seeds of lettuce cultivars

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Fernando Araújo De Almeida ◽  
Renata Silva-Mann ◽  
Heloisa Oliveira Dos Santos ◽  
Rucyan Walace Pereira ◽  
Arie Fitzgerald Blank
Keyword(s):  
Pyruvate Decarboxylase ◽  
Breeding Programs ◽  
Lettuce Seeds ◽  
Randomized Design ◽  
Different Temperatures ◽  
Lettuce Breeding ◽  
Physiological Quality ◽  
Biochemical Quality ◽  
Completely Randomized Design

The objective of this study was to evaluate the physiological quality allied to biochemical quality of lettuce seeds by germination and enzymes expression at 20, 25, 30, 35, 40 and 42ºC. Germination speed index and percentage of germination were estimated. Isoenzyme expressions were assessed by alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), catalase (CAT), esterase (EST), pyruvate decarboxylase (PDC) and glutamate oxaloacetate transferase (GOT). The experiment consisted of a completely randomized design in a factorial scheme 4x6, with four cultivars and six different temperatures, with four replications. The highest germination and vigor were observed for cv. ‘Everglades’ at 35°C, which proved that this cultivar is thermotolerant. Catalase can be considered a genetic marker for the identification of thermotolerant lettuce cultivars. Cultivar ‘Everglades’ has potential to be used in lettuce breeding programs aimed at cultivars tolerant to high temperatures during germination.    

scholarly journals Physiological performance of Coccoloba gigantifolia seeds subjected to desiccation and storage

2021 ◽  
Vol 43 ◽  
Author(s):  
Sidney Alberto do Nascimento Ferreira ◽  
Daniel Felipe de Oliveira Gentil ◽  
Carlos Alberto Cid Ferreira
Keyword(s):  
Moisture Content ◽  
Marked Reduction ◽  
Control Treatment ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Storage ◽  
Storage Temperatures ◽  
Water Contents

Abstract: Coccoloba gigantifolia is recently described species, with an area of restricted occurrence and threatened with extinction, whose great ornamental potential is due to the size of its leaves and the architecture of the plant. The objective of this study was to evaluate the viability of its seeds under different water contents and storage temperatures, through two experiments. The first one used a completely randomized design (CRD) with six treatments (drying periods: 0, 1, 2, 3, 4 and 5 days) and four replications. The second experiment used a CRD in a 2 (storage temperatures: 8.0 ± 1.2 and 19.5 ± 0.7 °C) x 3 (storage periods: 1, 2 and 4 months) factorial scheme, in addition to a control treatment (without storage), with four replications. The seeds have a recalcitrant behavior, as they are sensitive to desiccation, with a marked reduction in viability with moisture content below 19.5%. Storage at temperatures of 8.0 and 19.5 °C did not maintain the physiological quality of the seeds, with 25.2% water, and the largest losses were found at a temperature of 8.0 oC.

scholarly journals Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Dry Matter ◽  
Folk Medicine ◽  
Germination Percentage ◽  
Climate Variations ◽  
Randomized Design ◽  
Mother Plants ◽  
Physiological Quality ◽  
Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

scholarly journals Temperatures and periods of drying delay and quality of corn seeds harvested on the ears

2019 ◽  
Vol 41 (3) ◽  
pp. 336-343
Author(s):  
Everson Reis Carvalho ◽  
Victor Moss Francischini ◽  
Suemar Alexandre Gonçalves Avelar ◽  
Júlia Camargos da Costa
Keyword(s):  
Processing Unit ◽  
High Moisture Content ◽  
High Moisture ◽  
Seed Processing ◽  
Randomized Design ◽  
Delay Times ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Corn Hybrid

Abstract: Seeds harvested on the ears have high moisture content. On that account, this study aimed at evaluating the loss of physiological quality of corn seeds harvested on the ears, as a function of different drying-delay times. Hybrid corn ears were harvested at 31% moisture and then had their drying postponed for 0, 12, 24 and 36 h, while subjected to temperatures of 30, 40, 50 and 60 °C. The physiological quality was evaluated after 0, 4, 8 and 12 months of storage. A completely randomized design was employed, in a 4 x 4 x 4 factorial scheme, with four replications. In addition, a study was performed in a seed-processing unit, reporting the average waiting time before drying and the temperatures of all loads of a corn hybrid received at the facility. The physiological quality was not affected by temperatures below 40 °C, considering 36 h of waiting before drying. At 50 ºC during the drying delay, the germination was impaired 36 h afterward, and the vigor was compromised after 24 h, with the damage effects intensifying as the storage advanced. At the temperature of 60 ºC, the deficits in germination and vigor occurred within the first hours of drying delay.

scholarly journals THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

2021 ◽  
Vol 5 (1) ◽  
pp. 11-17
Author(s):  
Febiasasti Trias Nugraheni ◽  
Erma Prihastanti ◽  
Endah Dwi Hastuti
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Storage Temperature ◽  
Germination Percentage ◽  
Plasma Radiation ◽  
Agricultural Commodity ◽  
Time Duration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Storage

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

scholarly journals Postharvest of ‘Tommy Atkins’ Mango Submitted to Coating of Chlorella sp.

2018 ◽  
Vol 10 (9) ◽  
pp. 225 ◽  
Author(s):  
Agda M. F. de Oliveira ◽  
Railene H. C. Rocha ◽  
Welligthon A. Guedes ◽  
George A. Dias ◽  
José F. de Lima ◽  
...  
Keyword(s):  
Food Safety ◽  
Fruit Quality ◽  
Room Temperature ◽  
Design Experiment ◽  
Consumer Health ◽  
Mango Fruit ◽  
Chlorella Sp ◽  
Randomized Design ◽  
Completely Randomized Design

The use of natural products as coatings to preserve the fruit quality during storage is an important step to maintain food safety for consumer health. The use of microalgae in coatings, therefore, may be promising in the preservation of mango. The present work had the objective to evaluate the effect of coatings based on Chlorella sp. on the postharvest preservation of ‘Tommy Atkins’ mango during storage at room temperature (23 °C). We carried out a completely randomized design experiment consisting of 0%, 1%, 2%, 3% and 4% of Chlorella sp., using 10 fruits per treatment (n = 10). Analyzing the L*, a* and, b* indices, in the peel and the pulp of the mango fruit, we observed a delay in the ripening with the increase of the biofilm concentration. The firmness of the pulp and maintenance of the organic acids of the fruits were higher in the treatments with a large amount of Chlorella sp. The use of biofilm with Chlorella sp. at 2% preserved the quality of ‘Tommy Atkins’ mango until ten days of storage, at 23 °C and 42% RH.

scholarly journals PHYSIOLOGICAL QUALITY OF Brachiaria brizantha SEEDS TREATED WITH FUNGICIDE AND INSECTICIDE

2018 ◽  
Vol 31 (3) ◽  
pp. 651-656
Author(s):  
ERIC FABIANO SERAGUZI ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
FERNANDA BRITO CARDOSO ◽  
ANA CARINA DA SILVA CÂNDIDO ◽  
CHARLINE ZARATIN ALVES
Keyword(s):  
Root Development ◽  
Dry Mass ◽  
Brachiaria Brizantha ◽  
Thiophanate Methyl ◽  
Physiological Characterization ◽  
Randomized Design ◽  
Phytotoxic Effect ◽  
Physiological Quality ◽  
Completely Randomized Design

ABSTRACT This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.

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