Analysis of seed vigor and germination of Toona ciliata M. Roem. var. australis

Abstract: Seed analysis tests should be performed under standard conditions for each species, so that the results can be reproducible anywhere. Seeds of the forest species Toona ciliata var. australis have been commercialized in Brazil despite no standard conditions for their analysis have been published. The objective was to determine basic information about temperature and photoperiod for the germination test and the imbibition time and volume of water for electrical conductivity. In the germination test the seeds were incubated at 15, 20, 25 and 30 °C with 0, 12 and 16 h of light. The electrical conductivity was evaluated up to 108 h after immersion of the seeds in 50 and 100 mL of deionized water. The conditions of 25 °C and 16 h of light provided higher amount of normal seedlings. At 15 °C all seeds remained hard regardless the photoperiod used, however, it became absent when light was added at higher temperatures. The electrical conductivity test should be performed with a volume of 50 mL and evaluated after 24 h of imbibition. It also showed a stronger correlation between normal seedlings, being a promising vigor evaluation test for this species.

Download Full-text

Assessment of Seed Vigor Tests for Crambe abyssinica

Journal of Agricultural Science ◽

10.5539/jas.v10n12p527 ◽

2018 ◽

Vol 10 (12) ◽

pp. 527

Author(s):

Tássia Fernanda Santos Neri Soares ◽

Bruno Antônio Lemos de Freitas ◽

José Geraldo de Araújo Ferreira Filho ◽

Denise Cunha Fernandes dos Santos Dias ◽

Camila Andrade Fialho ◽

...

Keyword(s):

Electrical Conductivity ◽

Water Stress ◽

Stress Test ◽

Primary Root ◽

Cold Test ◽

Seed Vigor ◽

Stress Tests ◽

Germination Test ◽

Crambe Abyssinica ◽

Seedling Length

Vigor tests are important mainly to identify differences associated to lots performance in field conditions. The objective of this study was to define the most appropriate tests to evaluate the crambe (Crambe abyssinica) seeds vigor. Seven seed lots of crambe were subjected to the standard germination (on the first and last count), germination speed index (GSI), primary root protrusion speed index (PRPSI), seedling length dry and weight seedling, water stress test using PEG 6000 at -0.2 MPa, electrical conductivity and cold test without soil. Crambe lots studied had germination percentages varying from 54 to 80.5%. The GSI, on the first count of water stress and electrical conductivity test ranked lots between two vigor classes The PRPSI, seedling length, seedling dry weight and last count of water stress tests were less sensitive to evaluate vigor for crambe seed. The cold test without soil and the first count of standard germination test were capable to dishtinguish lots more efficiently than the standard germination test on last count by ranking four of the seven seed lots studied.

Download Full-text

Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

Download Full-text

Evaluation of the seedvigor of Tournefortia paniculata Cham. post-digestive tract of the ruby-crowned tanager Tachyphonus coronatus (Vieillot, 1822)

Ciência e Natura ◽

10.5902/2179460x36737 ◽

2019 ◽

Vol 41 ◽

pp. 15

Author(s):

Sérgio Thode Filho ◽

Heider Alves Franco ◽

Emanuele Nunes de Lima Figueiredo Jorge ◽

Mariana De Souza Oliveira ◽

Jhon Lenon Genovez de Oliveira ◽

...

Keyword(s):

Electrical Conductivity ◽

Digestive Tract ◽

Digestive System ◽

Forest Species ◽

Ecological Process ◽

Germination Test ◽

Individual Level ◽

Physiological Quality ◽

The Individual

Endozoochory (dispersion with passage through the digestive system of animals) is one of the most important processes. It is included in the ecological process known as dispersive mutualism, benefit the species involved. The most used method to evaluate the physiological quality of seeds is carried out based on the traditional germination test. However, electrical conductivity tests with seeds of forest species are still scarce, especially those developed with the individual method, which provide greater fidelity to the results so that through this one can define the test by the mass methods. The current work aimed to investigate the vigor, from the electrical conductivity of the seeds of Tournefortia paniculata Cham. after passage through the digestive tract of a ruby-crowned tanager Tachyphonus coronatus (Vieillot, 1822). An expedition was conducted in a fragmented area of Atlantic Forest in process of reforestation in the campus of the IFRJ. After the capture of the passerine with mist nets, proceeded the identification of seeds shed in its feces. The experimental design was completely randomized with one treatments with five replicates. The results allowed concluded that the seed of the T. paniculata Cham. after digestive tract of the ruby-crowned tanager presented high electrical conductivity in three of its replicates at the individual level.

Download Full-text

ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

Revista Caatinga ◽

10.1590/1983-21252021v34n406rc ◽

2021 ◽

Vol 34 (4) ◽

pp. 791-798

Author(s):

RAFAEL MARANI BARBOSA ◽

MATHEUS ANDRÉ DE JESUS ◽

RAFAELA ALVES PEREIRA ◽

GEDEON ALMEIDA GOMES JUNIOR

Keyword(s):

Electrical Conductivity ◽

Efficient Method ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Rice Seed ◽

Seed Sample ◽

Red Rice ◽

Germination Test ◽

Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

Download Full-text

Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

Download Full-text

SUBSTRATES AND TEMPERATURES FOR THE GERMINATION OF SEEDS OF Senegalia tenuifolia (L.) BRITTON & ROSE

Revista Caatinga ◽

10.1590/1983-21252016v29n113rc ◽

2016 ◽

Vol 29 (1) ◽

pp. 113-118 ◽

Cited By ~ 4

Author(s):

ALCIMONE MARIA SILVA ARAÚJO2 ◽

LIZ CAROLINA DA SILVA LAGOS CORTES ASSIS2 ◽

NARJARA WALESSA NOGUEIRA ◽

RÔMULO MAGNO OLIVEIRA DE FREITAS ◽

SALVADOR BARROS TORRES

Keyword(s):

Significant Interaction ◽

Dry Mass ◽

Shoot Length ◽

Forest Species ◽

Germination Test ◽

Tukey Test ◽

Seed Analysis ◽

Germination Speed ◽

Two Factors ◽

Different Temperatures

ABSTRACT: The Rules for Seed Analysis and the Instructions for Seed Analysis of Forest Species have no recommendations for conducting the S. tenuifolia germination test. Therefore, the aim of the present study was to evaluate different temperatures and substrates to perform the germination test of S. tenuifolia seeds. The experimental design was completely randomized in a factorial 4 × 3 design and consisted of four substrates (paper roll; on paper; on sand and on vermiculite) and three temperatures (25, 30 and 35 °C), with four replicates of 25 seeds. The percentage of normal seedlings, the germination speed index, the shoot length, root length and dry mass of seedlings were evaluated. The Tukey test was used at 5% probability. There was a significant interaction between the temperatures and substrates tested for all variables, indicating that there is at least one ideal combination of the two factors that can increase the germination of seeds. The germination and vigor of S. tenuifolia seeds are influenced by the temperature and by the substrate used in the germination test. The combination of the paper roll substrates with a temperature of 25 and 30 °C was suitable for the germination of S. tenuifolia seeds.

Download Full-text

Electrical conductivity of the seed soaking solution and soybean seedling emergence

Scientia Agricola ◽

10.1590/s0103-90162004000200007 ◽

2004 ◽

Vol 61 (2) ◽

pp. 164-168 ◽

Cited By ~ 27

Author(s):

Roberval Daiton Vieira ◽

Angelo Scappa Neto ◽

Sonia Regina Mudrovitsch de Bittencourt ◽

Maristela Panobianco

Keyword(s):

Electrical Conductivity ◽

Water Content ◽

Seedling Emergence ◽

Soybean Seed ◽

Accelerated Aging ◽

Seed Vigor ◽

Soybean Seedling ◽

Seed Soaking ◽

Laboratory Conditions ◽

Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

Download Full-text

Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000100006 ◽

2013 ◽

Vol 35 (1) ◽

pp. 42-50 ◽

Cited By ~ 1

Author(s):

Fábio Mielezrski ◽

Julio Marcos-Filho

Keyword(s):

Electrical Conductivity ◽

Growth Habit ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Storage ◽

Seed Vigor ◽

Seedling Length ◽

Pea Seeds ◽

Cold Chamber ◽

Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

Download Full-text

ELECTRICAL CONDUCTIVITY AND QUALITY OF DIFFERENT UMBEL ORDER CARROT SEEDS

Revista Brasileira de Agropecuária Sustentável ◽

10.21206/rbas.v9i04.8079 ◽

2020 ◽

Vol 9 (04) ◽

pp. 75-81

Author(s):

Luana de Souza Marinke ◽

Hugo César Rodrigues Moreira Catão ◽

Gabrielly Fernanda Francisco ◽

Ítala Menegon Castilho ◽

Letícia Rodrigues de Oliveira ◽

...

Keyword(s):

Electrical Conductivity ◽

Germination Rate ◽

Statistical Design ◽

Germination Test ◽

Aerial Portion ◽

Initial Characterization ◽

Different Temperatures ◽

Physiological Quality ◽

Final Score

Carrot seeds exhibit irregular vigor due to the wide blooming period of the crop. Results from germination tests are insufficient to evaluate the physiological potential. Thus, vigor tests are used in combination with germination tests. The objective of this work was to evaluate the quality of carrot seeds from different umbel order and the electrical conductivity under different temperatures and imbibing periods. Carrot seeds from cultivar Brasilia were used to perform the experiments. Content of water, the first and the final score of the germination test, index of germination rate, and length of roots and of the aerial portion were determined in the initial characterization. The electrical conductivity test was completed with replicates containing 50 seeds, in 25 mL deionized water at 20, 25 and 30oC. Evaluations were performed with 2, 4, 6, 8, 12 and 24 hours of imbibing period. The experiment was accomplished under a completely random statistical design. The quality of carrot seeds varied according to the umbel order. Seeds of secondary umbels showed better physiological quality. The electrical conductivity test is efficient to evaluate the vigor of carrot seeds with 8 hours of imbibing period at 25°C.

Download Full-text

Presence of glyphosate can harm the germination of bean seeds treated with biostimulant

Bioscience Journal ◽

10.14393/bj-v36n1a2020-42441 ◽

2020 ◽

Vol 36 (1) ◽

Author(s):

Jorgiani de Ávila ◽

Fernanda Brito Cardoso ◽

Sebastião Ferreira de Lima ◽

Gustavo Ribeiro Barzotto ◽

Mayara Santana Zanella

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Cold Test ◽

Seed Vigor ◽

Common Beans ◽

Accelerated Aging Test ◽

Root And Shoot Length ◽

Aging Test ◽

Early Seedling ◽

Different Doses

It is possible to cultivate common beans for a third harvest in one agricultural year due to varietal characteristics. For calendar adequacy, performing desiccation and planting often occur almost simultaneously. Germination performance of many plant species can improve with biostimulant use on seeds, however the interaction with herbicide residual molecules is unknown. The hypothesis is that seeds treated with a biostimulant in soil with glyphosate residues can eliminate the advantage of the biostimulant or increase the damage caused by the herbicide. The aim of this study was to evaluate the effect of different glyphosate reduce doses and the interaction with biostimulant on bean seed germination and vigor. The experiment was conducted using a completely randomized 2 x 5 factorial block designing factorial 2 x 5, corresponding to the presence and absence of biostimulant and five different doses of glyphosate reduce rates, with four repetitions. The conducted evaluations were first count germination, germination test, accelerated aging, cold test, root and shoot length, root and shoot dry matter and electrical conductivity. It can be concluded that the biostimulant treatment on bean seeds increased germination, seed vigor and early seedling growth, but glyphosate presence reduced those advantages, increasing electrical conductivity. However, the herbicide presence provided higher germination on the accelerated aging test.

Download Full-text