scholarly journals PHYTOCHEMICAL PROFILE AND GASTROPROTECTIVE ACTIVITY OF EUGENIA MATTOSII FRUITS

2018 ◽  
Vol 55 (2) ◽  
pp. 138-141 ◽  
Author(s):  
Luana dos SANTOS ◽  
Adriana CAMPOS ◽  
Valdir CECHINEL FILHO ◽  
Luciane Angela Nottar NESELLO
Keyword(s):  
Therapeutic Potential ◽  
Conventional Medicine ◽  
Gastrointestinal Diseases ◽  
Experimental Models ◽  
Phytochemical Analysis ◽  
Gastroprotective Activity ◽  
Lesion Area ◽  
Acute Ulcer ◽  
Ulcer Model ◽  
Phytochemical Profile

ABSTRACT BACKGROUND: Extracts obtained from plants and fruits provide a relatively safe and practical alternative for the conventional medicine of gastrointestinal diseases. The specie Eugenia mattosii, popularly known in Brazil as “cerejinha”, belongs to Myrtaceae family. Species of this family present pharmacological properties, and can be used in the treatment of gastrointestinal disorders. OBJECTIVE: The aim of this study was to determine the phytochemical profile and evaluate the gastroprotective activity of Eugenia mattosii fruits. METHODS: Phytochemical analysis was carried out by thin layer chromatography and gastroprotective assays were performed using two experimental models: acute ulcer model induced by ethanol/HCl and acute ulcer model induced by non-steroidal anti-inflammatory drug (indomethacin). Total lesion area (mm2) and relative lesion area (%) were determined. RESULTS: The results of the phytochemical analysis indicated that the bark and pulp and seeds of E. mattosii present phenolic compounds, terpenes and/or steroids. In gastric ulcer model induced by ethanol was evidenced significant reduction of damaged areas for doses of 50 and 250 mg/ kg of seeds methanol extract, while in the indomethacin-induced ulcer model, all parts of the fruit presented defense capability of the gastric mucosa by reducing lesions at doses of 50, 125 and 250 mg/kg. CONCLUSION: The results demonstrate that the specie E. mattosii has bioactive compounds that provide gastroprotective activity, presenting possible therapeutic potential.

scholarly journals SCREENING OF WILD FRUIT TREES WITH GASTROPROTECTIVE ACTIVITY IN DIFFERENT EXPERIMENTAL MODELS

2017 ◽  
Vol 54 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Luciane Angela Nottar NESELLO ◽  
Adriana CAMPOS ◽  
Roseane Leandra da ROSA ◽  
Sérgio Faloni de ANDRADE ◽  
Valdir CECHINEL FILHO
Keyword(s):  
Gastric Ulcer ◽  
Fruit Trees ◽  
Experimental Models ◽  
Gastrointestinal Disorders ◽  
Gastroprotective Activity ◽  
Lesion Area ◽  
Wild Fruit ◽  
Ulcer Model ◽  
Different Parts

ABSTRACT BACKGROUND Given the increase of people with gastrointestinal disorders, the search for alternative treatments with fewer side effects is vital, as well as the demand for food or plants that can help protect the stomach. OBJECTIVE The aim of this study was to evaluate the gastroprotective action of the extracts of wild fruit trees of Myrcianthes pungens (guabiju); Inga vera Willd. (ingá-banana) and Marlierea tomentosa Cambess. (guarapuruna) in in vivo pharmacological models. METHODS The different parts of the fruits were separately subjected to a process of extraction by methanol. Two experimental pharmacological models were conducted in mice; the gastric ulcer model induced by non-steroidal anti-inflammatory (indomethacin), and the gastric ulcer model induced by ethanol/HCl, which allowed us to evaluate the gastroprotective activity of the extracts at a dose of 250 mg/kg. Subsequently, the total lesion area (mm2) and relative lesion area (%) were determined. RESULTS The results showed significant gastroprotective activity against the aggressive agents used - ethanol and indomethacin - for all the extracts tested. CONCLUSION It is assumed that the fruits have bioactive compounds such as antioxidant substances that act on the prostaglandin levels, protecting them from the damage caused by ethanol and indomethacin. These results prompt further studies to isolate and identify the active properties.

scholarly journals Antioxidant and gastroprotective effects of the ethyl acetate extract from Stemodia maritima L. in ethanol-induced gastric ulcer model

2021 ◽  
Vol 10 (15) ◽  
pp. e195101522548
Author(s):  
Rayane Siqueira de Sousa ◽  
Jéssica de Andrade Gomes Silva ◽  
Elizabeth Fernanda de Oliveira Borba ◽  
Katharina Rodrigues de Lima Porto Ramos ◽  
Camila Joyce Alves da Silva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gastric Ulcer ◽  
Acute Toxicity ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Control Group ◽  
Gastroprotective Activity ◽  
Ulcer Model ◽  
Phytochemical Profile

Stemodia maritima L., is a shrub of the Plantaginaceae family, with some biological activities already described, such as: larvicide, antimicrobial, and anti-inflammatory. Thus, the objective of this work was to evaluate the antioxidant, and gastroprotective activities of the ethyl acetate extract from S. maritima. The phytochemical profile was investigated through the quantification of total phenolic compounds, flavonoids, and CCD analysis. The toxicity of the extract was performed through cell viability using L929 line cell, and acute toxicity by the OECD Guide 423. The antioxidant activity was analyzed by the methods of reduction of the ferric ion (FRAP), total antioxidant activity (TAA), and the gastroprotective activity by the absolute ethanol-induced gastric ulcer model, with analysis of NO, MDA, GSH and MPO levels in the stomach tissues. In the phytochemical profile it was possible to identify the presence of flavonoids, triterpenes, steroids, mono, and sesquiterpenes. The extract was not cytotoxic against L929 lineage, maintaining cell viability above 70% at the doses tested, and in acute toxicity it did not show physiological changes indicative of toxicity compared to the control group. The extract presented antioxidant activity of 157.3 ± 9.7 mg equivalent of Trolox/g of extract in the FRAP method, and 50.0 ± 1.1 % by TAA. The ethyl acetate extract of S. maritima, at the doses tested, reduced the ulcerative lesion index compared to the injured control group, increased the levels of NO and GSH, and was able to decrease the concentrations of MDA and MPO, enhancing their gastroprotective activity.

scholarly journals Study of the Gastroprotective Effect of Extracts and Semipurified Fractions ofChresta martiiDC. and Identification of Its Principal Compounds

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
E. S. Franco ◽  
M. E. B. Mélo ◽  
B. J. A. Jatobá ◽  
A. L. B. D. Santana ◽  
A. A. R. Silva ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Gastrointestinal Diseases ◽  
Gastric Ulcers ◽  
Resonance Spectroscopy ◽  
Gastroprotective Activity ◽  
H Nmr ◽  
Aerial Parts ◽  
Organic Extracts ◽  
Amorphous Compounds ◽  
Ulcer Model

Chresta martii(Asteraceae) is a species widely used by the population of the Xingu region of Sergipe, Brazil, in the form of a decoction (aerial parts) for the treatment of gastrointestinal diseases. The study aims to assess the gastroprotective activity of organic extracts and semipurified fractions and identify the principal compounds present inC. martiiresponsible for such activity. The organic extracts (cyclohexane: ECCm, ethyl acetate: EACm, and ethanol: EECm) were obtained from the dried aerial parts (500 g) ofC. martii. For evaluation of the gastroprotective activity of extracts (50, 100, or 200 mg/kg; p.o.), maleSwiss Webstermice (25–30 g) were used which had gastric ulcers induced by indomethacin (40 mg/kg, s.c.) or ethanol (0.2 mL/animal; p.o.). Among the extracts evaluated, EACm exhibited significant (P<0.05) gastroprotective activity in the models used. The fractionation of EACm was performed in a silica gel column 60 eluted with the following compounds: [chloroform—F1 yield (10%)], [chloroform/ethyl acetate (1/1)—F2 yield (6%)], [ethyl acetate—F3 yield (8%)], and [ethyl/methanol acetate (1/1)—F4 yield (5%)]. Of the fractions described above, the F1 (25 mg/kg; p.o.) had greater gastroprotective activity (P<0.05) than that displayed by ranitidine (80 mg/kg; p.o.) in the ethanol-induced ulcer model. The refractionation of F1 produced 23 subfractions and from these two yellow amorphous compounds were obtained by recrystallization, Rf: 0.46 and 0.31 (ethyl acetate : chloroform 5 : 5). The compounds isolated were characterized by nuclear magnetic resonance spectroscopy (1H-NMR and13C-NMR) and identified as flavones: chrysoeriol (yield: 0.43%) and 3′,4′-dimethoxyluteolin (yield: 0.58%).Conclusion. Flavone 3′,4′-dimethoxyluteolin is the principal compound present in the speciesC. martiiand is probably responsible for gastroprotective activity observed in this species.

scholarly journals Phytochemical Analysis and Gastroprotective Activity of the Root Bark from Maytenus robusta

2016 ◽  
Vol 11 (5) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Danyela Francine Benvenutti ◽  
Franco Delle Monache ◽  
Valdir Cechinel Filho ◽  
Sérgio Faloni de Andrade ◽  
Rivaldo Niero
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Pharmacological Effects ◽  
Gastroprotective Activity ◽  
Lesion Area ◽  
Pure Compounds ◽  
Promising Source ◽  
Lesion Index

The present work evaluated the chemical composition and antiulcerogenic potential of the crude extract, fractions and pure compounds isolated of roots barks from Maytenus robusta Reiss, using different pharmacological models in mice. 3,12-Dioxofriedelane (1) and 11-hydroxylup-20 (29)-en-3-one (2) were isolated from the n-hexane fraction, and mayteine (3) and 3,7-dioxofriedelane (4) from the dichloromethane fraction. The crude extract (50, 259, 500 mg/kg), all the fractions (250 mg/kg) and cimetidine (100 mg/kg) significantly reduced the lesion index, total lesion area, and percentage of lesions, in comparison with the control group ( p< 0.05), by ethanol and NSAID-induced ulcer models. All the isolated compounds also presented significant pharmacological effects at 30 mg/kg. These results show that the root bark of M. robusta may be a promising source of molecules with applicability in the treatment of gastric disorders.

scholarly journals Phytochemical Profile and Investigation of the Spasmolytic Activity of Hydroalcoholic Extract of Syzygium cumini (L.) Skeels Seeds

2020 ◽  
pp. 27-38
Author(s):  
F. S. Monteiro ◽  
A. F. S. Carvalho ◽  
R. M. Ribeiro ◽  
A. C. R. Borges ◽  
M. O. R. Borges
Keyword(s):  
Saline Solution ◽  
Therapeutic Potential ◽  
Smooth Muscles ◽  
Phytochemical Analysis ◽  
Spasmolytic Activity ◽  
Tonic Component ◽  
Hydroalcoholic Extract ◽  
Phytochemical Profile ◽  
Value Effect ◽  
Stabilization Period

Aims: Perform the phytochemical analysis and investigate the spasmolytic activity of the hydroalcoholic extract obtained from S. cumini seeds (EHS-SC). Study Design: Qualitative phytochemical analysis and test of the EHS-SC on isolated smooth muscles (aorta, trachea, jejunum and uterus) of rat, to value effect relaxant and/or inhibitor. Place and Duration of Study: Pharmacognosy Laboratory II (Pharmacy course) and Pharmacology Research and Post-Graduate Laboratory (Department of Physiological Sciences) of the Federal University of Maranhão, between January 2017 and July 2018. Methodology: EHS-SC was submitted to phytochemical analysis and changes in color, fluorescence and absence or presence of precipitate were observed. The smooth muscle segments were suspended (tension of 1 g) in glass vats containing specific saline solution, at an appropriate temperature and after stabilization period, was stimulated by a suitable contractile agent to observe the effect of EHS-SC in the phasic and/or tonic component. Results: EHS-SC showed the majority presence of phenols, steroids, alkaloids and flavonoids (flavones, xanthones, flavonols) and was more potent in inhibiting phasic contractions induced by 10-6 M carbachol (CCh) in isolated rat jejunum (Emax: 83.5 ± 6.7%; n = 3). In addition, the EHS-SC (81.0; 243.0 and 729 µg/mL) antagonized the CCh effect (n = 4), increasing the EC50 (6.5 ± 1.3 x 10-7 M) of the CCh to 8.5 ± 1.1; 18.5 ± 3.4 and 40.5 ± 7.4 x 10-7 M and reducing the Emax (100%) of the CCh to 82.9 ± 10.5; 67.6 ± 6.0 and 10.1 ± 8.3%. Conclusion: Spasmolytic activity may be combined with antimicrobial and antidiarrheal activity according to literature data, where they show that the seeds have the same secondary metabolites, signaling the therapeutic potential for the treatment of colic and/or diarrhea.

scholarly journals GT-Repeat Polymorphism in the HO-1 Gene Promoter Is Associated with Risk of Liver Cancer: A Follow-Up Study from Arseniasis-Endemic Areas in Taiwan

2021 ◽  
Vol 10 (7) ◽  
pp. 1489
Author(s):  
Meei-Maan Wu ◽  
Fang-I Hsieh ◽  
Ling-I Hsu ◽  
Te-Chang Lee ◽  
Hung-Yi Chiou ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Risk ◽  
Cox Regression ◽  
Therapeutic Potential ◽  
Gene Promoter ◽  
Experimental Models ◽  
Heme Oxygenase 1 ◽  
Cox Regression Analysis ◽  
S Alleles

The induction of heme oxygenase-1 (HO-1) has been shown to have therapeutic potential in experimental models of hepatitis and liver fibrosis, which are closely related to liver cancer. In humans, HO-1 induction is transcriptionally modulated by the length of a GT-repeat [(GT)n] in the promoter region. We aimed to investigate the effect of HO-1 (GT)n variants on liver cancer in a human population. We determined the HO-1 genotype in 1153 study subjects and examined their association with liver cancer risk during a 15.9-year follow-up. Allelic polymorphisms were classified as short [S, <27 (GT)n] or long [L, ≥27 (GT)n]. Newly developed cancer cases were identified through linkage to the National Cancer Registry of Taiwan. Multivariate Cox regression analysis was used to evaluate the effect of the HO-1 (GT)n variants. Alpha-fetoprotein (AFP) and cirrhosis history were also examined. The S/S genotype was found to be significantly associated with liver cancer risk, compared to the L/S and L/L genotypes. The S/S genotype group also had a higher percentage of subjects with abnormal AFP levels than other groups. There were significant percentages of cirrhosis among groups who carried S-alleles. Our findings indicate that short (GT)n variants in the HO-1 gene may confer susceptibility to rather than protection from liver cirrhosis/cancer.

scholarly journals A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Soudeh Moghadasi ◽  
Marischa Elveny ◽  
Heshu Sulaiman Rahman ◽  
Wanich Suksatan ◽  
Abduladheem Turki Jalil ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Ethical Issues ◽  
Therapeutic Potential ◽  
Kidney Diseases ◽  
Experimental Models ◽  
Live Cells ◽  
Target Cells ◽  
Intercellular Interaction ◽  
Micro Rnas

AbstractRecently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs are not related to their differentiation potentials, but rather are exerted through the release of soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, and owning targeting capability and organotropism, exosomes are considered nanocarriers for their possible use in diagnosis and therapy. Exosomes convey functional molecules such as long non-coding RNAs (lncRNAs) and micro-RNAs (miRNAs), proteins (e.g., chemokine and cytokine), and lipids from MSCs to the target cells. They participate in intercellular interaction procedures and enable the repair of damaged or diseased tissues and organs. Findings have evidenced that exosomes alone are liable for the beneficial influences of MSCs in a myriad of experimental models, suggesting that MSC- exosomes can be utilized to establish a novel cell-free therapeutic strategy for the treatment of varied human disorders, encompassing myocardial infarction (MI), CNS-related disorders, musculoskeletal disorders (e.g. arthritis), kidney diseases, liver diseases, lung diseases, as well as cutaneous wounds. Importantly, compared with MSCs, MSC- exosomes serve more steady entities and reduced safety risks concerning the injection of live cells, such as microvasculature occlusion risk. In the current review, we will discuss the therapeutic potential of MSC- exosomes as an innovative approach in the context of regenerative medicine and highlight the recent knowledge on MSC- exosomes in translational medicine, focusing on in vivo researches.

scholarly journals Therapeutic Features and Updated Clinical Trials of Mesenchymal Stem Cell (MSC)-Derived Exosomes

2021 ◽  
Vol 10 (4) ◽  
pp. 711
Author(s):  
Byung-Chul Lee ◽  
Insung Kang ◽  
Kyung-Rok Yu
Keyword(s):  
Clinical Trials ◽  
Therapeutic Agent ◽  
Therapeutic Potential ◽  
Therapeutic Option ◽  
Treatment Options ◽  
Genetic Material ◽  
Experimental Models ◽  
Attractive Alternative ◽  
Cell Based Therapy ◽  
Cellular Rejection

Identification of the immunomodulatory and regenerative properties of mesenchymal stem cells (MSCs) have made them an attractive alternative therapeutic option for diseases with no effective treatment options. Numerous clinical trials have followed; however, issues such as infusional toxicity and cellular rejection have been reported. To address these problems associated with cell-based therapy, MSC exosome therapy was developed and has shown promising clinical outcomes. MSC exosomes are nanosized vesicles secreted from MSCs and represent a non-cellular therapeutic agent. MSC exosomes retain therapeutic features of the cells from which they originated including genetic material, lipids, and proteins. Similar to MSCs, exosomes can induce cell differentiation, immunoregulation, angiogenesis, and tumor suppression. MSC exosomes have therefore been employed in several experimental models and clinical studies. Here, we review the therapeutic potential of MSC-derived exosomes and summarize currently ongoing clinical trials according to disease type. In addition, we propose several functional enhancement strategies for the effective clinical application of MSC exosome therapy.

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